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Methods: The three lung cancer cell lines-A549(highly invasive human lung adenocarcinoma cell ), 95-D(highly metastatic human lung carcinoma cell), and SPC-A-1(lowly metastatic human lung adenocarcinoma cell) were cultured conventionally in vitro. When the cells covered the bottom of culture flask, we abstracted the protein with lysis solution containing RIPA and PMSF, and obtained RNA with RNeasy Mini Kit.

将三株肺癌细胞：高侵袭的肺腺癌细胞(A549)、高转移的肺癌细胞(95-D)、低转移的肺腺癌细胞(SPC-A-1)做常规细胞培养，待细胞长满瓶底后，用RIPA和PMSF组成的细胞裂解液提取细胞蛋白质，RNeasy Mini Ki提取RNA。

These 375 patients had a median age of 50.57±10.46(range,19-72) with 87.4%HBsAg positive and 4.3%anti-HCV antibody positive;The apparent peak incidence age was 40～60 years old,and the ratio of male to female was 10.7:1;The 3 and 5 year postoperational survival rate were 52%and 38%;The tumour numbers(p=0.000),tumor size(p=0.025),histological pattern (p=0.000),nuclear features(p=0.000),differentiation(p=0.001) and vascular invasion(p=0.000) were significantly correlated with prognosis.The postoperational survival time of thin trabeculae pattern,compact pattern and pseudoglandular pattern were significantly longer than that of thick trabeculae, scirrhous pattern,and solid patternp＜0.009the postoperational survival time of 1 and 2 grade based on nuclear features were significantly longer than that of 3 and 4 grades(p=0.000The small cell variant,osteoclast-like giant cell variant, and spindle cell variant were mainly composed of thick trabeculae pattern and solid pattern,which were significantly different from that of clear cell variant.

结果1。本组资料显示肝细胞癌发病年龄19～72岁，平均50.57±10.46岁，发病高峰年龄40～60岁，男女比例为10.7：1；HBsAg87.4%，anti-HCV抗体4.3%；术后3年生存率为52%，5年生存率为38%；肿瘤数目(p=0.000)、肿瘤大小(p=0.025)、组织学结构类型(p=0.000)、核分级(p=0.000)、分化程度(p=0.001)及血管浸润均(p=0.000)与预后明显相关；其中组织学结构类型中细梁状型生存时间与致密梁状型和腺样型无明显区别(p＞0.05)而明显高于粗梁状型、实性型和硬化型(p≤0.009)，硬化型生存时间与实性型之间无明显区(p＞0.05)而明显低于其余各型p≤0.006核分级1级与2级生存时间无明显区别(p＞0.05，核分级3级与4级生存时间无明显区别(p＞0.05)，而核分级3级生存时间明显低于2p=0.000小细胞型、巨细胞型和梭形细胞型主要由实性型和粗梁状型组织学结构类型构成，明显不同于透明细胞型(主要由细梁状型和粗梁状型构成(p≤1.006)。2。

The first split of microspore mother cell is observed after one week after beginning of temperature arised under high temperature but the split of secondary sporogenous cell doesn't be found. The split of secondary sporogenous cell is still found after one week but the first split of microspore mother cell is observed after 12d under low temperature. Formating speed of male gametophyte is affected by temperature.

在高温条件下，开始加温1周后即看到小孢子母细胞进行第一次分裂，未观察到次生造孢细胞的分裂；在低温条件下，1周后仍能看到次生造孢细胞的分裂，12d后才看到小孢子母细胞进行第一次分裂。

Under normal temperature, anthers of not fertility restoration stopped developing in the stage of archesporical cell; 1-2 comers of a few anther could differentiate and form archesporical cell, sporogenous cells and microspore mother cell, but microspore mother cell disintegrated on the first meiotic division.

常温下，未发生育性恢复的花药在孢原细胞期就停止发育；少数花药1~2个角隅处可分化形成孢原细胞，造孢细胞，花粉母细胞，但花粉母细胞在减数分裂的第一次分裂时解体。

Based on a lot of experiments, it shows the distinct error between classical theory curve which express the relation of cell diameter, cell density and foam tensile strength , tensibility tensile product and re-spring degree and experiment curve. It also can＇t explain the relation of hole degree , area of cell wall with above four mechanics parameter. But the sub-cluster scale theory was demonstrated successfully to explain the relation of cell structure and foam mechanics property on different mold temperature, mold pressure, mold period and different chemical blowing agent, blowing activator content.

通过大量的实验结果理论分析表明：以往的经典理论存在着很多不足之处，它不能有效地解释孔径、泡孔密度与抗拉强度、断裂伸长率、断裂功、及回弹率之间的理论曲线与实验曲线之间的偏差，也不能解释空隙度、泡孔壁厚与上面四者之间的关系，而群子标度理论则成功地论证了在不同模压温度、模压压力、模压时间及不同用量发泡剂、发泡促进剂条件下泡体力学性能与结构之间的相关性。

Methods Human prostate cancer PC-3 cell, breast cancer ER-75-30 cell and lung cancer A549 cell were cultured in RPMI medium 1640.Light microscope,transmissional electron microscope, flow cytometer and immunohistochemical method were used to observe apoptosis morphological changes of three kinds of tumor cell lines, time and dose effect relationship and to detect expression of bcl-2 and bax.

方法人前列腺癌 PC-3细胞株、乳腺癌 ER-75-30细胞株和非小细胞肺癌 A549细胞株经培养后，采用光镜、电镜、流式细胞仪和免疫组化方法研究 188Re诱导 3种肿瘤细胞凋亡的形态学变化、时间剂量效应关系、细胞周期依赖性以及凋亡相关基因 bcl-2和 bax在其中的表达情况。

In this thesis, we find that dlg is indispensible in the establishment of anterior-posterior and dorsal-ventral polarity of drosophila oocyte. Removal of Dlg function from the posterior follicle cells using the FLP/FRT system leads to disruption of oocyte skeleton reconstruction that is elicited by the failure of those posterior cells to differentiate normally in mid-oogenesis. We demonstrate that abnormity of Notch, JAK-STAT and EGFR signal pathway in dlg mutants contributes to this aberrant differentiation. dlg null mutant also blocks the normal differentiation of two groups of anterior follicle cell-stretched cell and centripetal cell, but not border cell, with a lower penetrance. However unlike the result in posterior follicle cells, Notch and JAK-STAT signaling are both undisrupted in all mutant anterior follicle cells, implying other fate determinants may be involved.

我们的研究发现，后端滤泡细胞中的Dlg在果蝇卵子发生中期卵母细胞前后轴和背腹轴建立过程中也是必须的，PFC中dlg完全缺失型突变引起PFC的分化异常，导致卵子发生中期卵母细胞骨架重组异常，Stau、Grk等极性决定蛋白定位错误。dlg突变阻碍了Notch、JAK-STAT、EGFR等调节PFC分化的信号通路的激活。dlg突变的PFC也没有获得前端滤泡细胞命运。dlg突变不影响前端滤泡细胞群中边界细胞的分化，但是在一定程度上影响伸展细胞和向心细胞的分化，并且这种影响不依赖于前端滤泡细胞Notch或JAK-STAT信号激活的异常。

A 1: Uninduced supernant of cell lysate by sonication; 2: Induced supernant of cell lysate by sonication; 3: Uninduced precipitate of cell lysate by sonication; 4: Induced inclusion body of cell lysate by sonication; 5: Purified ULBP4 protein; M: Low molecular marker proteins.

2.3 体外NKG2D结合实验和细胞因子分泌实验间接ELISA检测结果显示：原核表达复性后的ULBP4能与人NKG2D结合，而带His标签的无关蛋白则不能与之结合，说明复性后的ULBP4仍能维持正确的天然构像。

Results Using different concentrations of 5fluorouracil to deal with A549 cells, with the increase in drug concentration and treatment time,cell survival rate decreased significantly;and low concentration of 5fluorouracil induced the cell spread and increased vacuolization within the cytoplasm, acridine orange staining showed an increase in acidic vesicles,high concentration made the cell round and cell apoptosis.

结果不同浓度的5氟尿嘧啶处理A549细胞，随着药物浓度的增加和处理时间的延长，细胞存活率明显下降；且低浓度使细胞呈铺展状态，细胞质内空泡增多，吖啶橙染色显示酸性膜泡增多，高浓度使细胞变圆，出现凋亡状态。

The experiment showed:CQP can reduce lipid peroxid a injury of myocardial cell membrane,can inhibit PLA2 activity,CQP may improv e the cell membrane fluidity,may prevent the damage of cell membrane,reduce the impairment of myocardial cell.

而CQP可减轻心肌细胞膜的脂质过氧化损伤，抑制PLA 2活性，改善细胞膜流动性，保护心肌细胞膜，减轻心肌细胞损伤。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。