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Impact of PCL capsulation on the mesenchymal stem cell activity studied in vivo, one plate culture with complete culture medium for control group with and two encapsulated transplantation for experimental groups(complete culture medium or basic culture medium for cell suspended) installed,after package and transplantation,retrieving capsulated cells for cell count,MTT assay,CCK-8 assay Daily for 8days to determine cell activity,and analysis growth curve.

以完全培养液平皿法细胞培养为对照组，设2个实验组进行聚己内酯囊管化骨髓间充质干细胞移植细胞活性研究。两个实验组分别以完全培养液和基础培养液悬浮细胞后封装移植，囊管内细胞回收后以细胞计数、MTT法、CCK-8法测定细胞的活性，共8天，进行生长曲线分析。

Observation using transmission electron microscopy found the irreversible centripetal movement of chloroplasts in chlorotic plants, as a result occurred in three sequential events:(1) chloroplast positions changed from face position (with long axis of chloroplasts at the cell walls perpendicular to light ray) to profile position (with long axis chloroplasts near the cell walls parallel to light ray),(2) chloroplast shapes changed from long elliptical to circular,(3) chloroplasts were separated from the cell wall and moved to the center of the cell.

透射电镜观察发现失绿叶片中的叶绿体发生不可逆的向心运动，整个过程大致经历以下几个阶段：(1)叶绿体从叶绿体长轴垂直于光线转变至叶绿体长轴平行于光线；(2)叶绿体的形状从椭圆形转变为圆形；(3)叶绿体脱离细胞壁向细胞中心运动。

Methods: morphological change observation, live cell accounting and clone formation were used to investigate the inhibitory effects of Cirsium setosum MB on human chronic myloid leukemia K562 cell, hepatoma HepG2cell, cervical carcinoma Hela cell and gastric carcinoma BGC823 cell.

细胞形态观察、活细胞计数法和克隆形成率法研究小蓟水提液对人白血病细胞K562、肝癌细胞HepG2、宫颈癌细胞Hela、胃癌细胞BGC823生长的抑制作用。

Based on these previous work, in this research, in order to explore the the molecular mechanism of enhanced tumor cell immunogenicity treated by elemene and mytomycin C compositely, we used murine Hca-F ascitic hepatic carcinoma ( high lymphatic metastasis substrain of H22) as an experimental model to compare the immunoprotective effects on Hca-F cells as well as the mechanism of the effects caused by HSP70-peptide complex purified from untreated tumor cells, EC-TCV and BCG; We studied the mechanism of increased tumor cell membrane HSP expression treated with elemene by the techniques of RT-PCR and immunoelectron microscopy; In order to deeply elucidate the antitumor effects and the molecular mechanism of elemene in increasing tumor cell immunogenicity we analyzed and compared the effects of elemene and heat shock on the gene expressions of tumor cell using gene expression profile chip.

本论文在上述研究成果的基础上，选择小鼠肝癌H22的高淋巴道转移亚系Hca-F为模型，进一步比较了未处理肿瘤细胞来源、榄香烯复合瘤苗来源的和卡介苗来源的HSP70-肽复合物对Hca-F的免疫保护效应、效应机制，探讨榄香烯-丝裂霉素复合处理增强肿瘤细胞免疫原性的分子机制；以Hca-F和人肝癌细胞株HepG〓等为模型研究了榄香烯处理增强肿瘤细胞膜热休克蛋白表达的机制；以HepG〓为模型用表达谱基因芯片研究比较了榄香烯与热休克处理对肿瘤细胞基因表达的影响，从而进一步阐明榄香烯抗肿瘤作用及增强肿瘤细胞免疫原性的分子机制。

In contrast to conventional methods, a novel cell sheet-based technique has been proposed .This technique intend to achieve high cell density and function by layering confluent viable cell sheets without the use of acellular scaffolds that limit mass transport. Using thermo-sensitive cell culture surfaces, confluently cultured cells spontaneously detach as intact sheets simply by reducing the culture temperature.

与传统方法相比，有学者提出了一种新的以细胞层为基础的组织工程技术，这种技术作为一种新的选择，利用温敏性培养皿，经简单的温度变化，可以非损伤性的一起获得完整的培养细胞层及其所沉积的细胞外基质，从而避免了使用蛋白水解酶。

Results: CK18 was not detected in normal and abnormal oral tissue sections. But in normal nonkeratinized mucosa, CK19 was detected in the basal cell layer dispersively. In epithelial dysplasia, CK19 was detected in the suprabasal cell layer and the number of CK19-positive cell layers was correlated with the dysplasia degree of epithelia. Furthermore, CK19 was detected in oral squamous cell carcinoma, especially in the poor-differentiated cancer cells.

结果：正常和异常的口腔粘膜均未检测到CK18；CK19可表达于正常非角化上皮的基底层细胞、异常增生上皮的基底上层细胞及口腔鳞状细胞癌中，阳性细胞数与上皮的异常增生程度及鳞癌的分化程度相关。

The present invention relates to a process of producing a functional immunoglobulin superfamily protein, which has at least one disulphide bond when functional, the process comprising the steps of providing a bacterial cell comprising a gene coding for the protein, the gene is expressible in said cell, cultivating the cell under conditions where the gene is expressed, isolating the protein from the cell without reducing it, and subjecting the isolated protein to a folding treatment.

本发明涉及一种产生功能性免疫球蛋白超家族蛋白的方法，此蛋白质在有功能时具有至少一个二硫键，此方法包括如下步骤：提供一种包含编码此蛋白质的基因且此基因可在其中表达的细菌细胞；在使此基因表达的条件下培养此细胞；从该细胞中分离出此蛋白质而不还原它；以及使分离出的蛋白质经受折叠处理。

As one of the most widely used technology in cell fusion, cell electrofusion is a rapidly developing cell engineering method, which utilizes dielectrophoretic force to induce cell alignment.

细胞电融合是一种发展迅速的细胞工程技术，在细胞融合研究领域得到了最广泛的应用。

In the normal uterus, Cytokeratins immunolabelling were detected in glandular cell, luminal epithelial cell, Vimentin immunolabelling were detected in stromal cell and endoblastic cell; CK7 immunolabelling were not detected in any tissue of the yak utenus.

研究结果显示：未妊娠时，泛角蛋白在子宫内膜腺上皮细胞、腔上皮细胞内表达，波形蛋白在子宫内膜基质细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，牦牛子宫任何部位均不表达角蛋白7；妊娠30天左右时，泛角蛋白在子宫内膜腺上皮细胞、子宫内膜腔上皮细胞、滋养层细胞、内胚层细胞和尿囊细胞内表达，波形蛋白在子宫内膜基质细胞和内胚层细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，角蛋白7在尿囊细胞内表达，偶尔在腔上皮细胞的细胞核边缘表达；消化法进行原代培养时，组织经胶原酶消化并通过100目和400目筛网组合可以有效地分离原代子宫内膜基质细胞和子宫内膜腺上皮细胞；分离得到的子宫内膜基质细胞活率达90%以上，并可在体外传代7次以上；分离得到的子宫内膜腺上皮细胞活率可达85%以上，并可在体外传代5次以上；RPMI1640培养基最适合子宫内膜基质细胞和子宫内膜腺上皮细胞的生长，维持子宫内膜基质细胞正常生长的FBS添加量为20%，维持子宫内膜腺上皮细胞正常生长的胎牛血清添加量为30%。

In order to analyze experimentally the back-scattering micro-spectrum difference between human normal stomach epithetic cell and the cancerous cell to provide optical technique for clinical checking,a micro-spectral measurement setup for checking cell was established based on fiber confocal microscopically imaging technique combined with cell light scattering theory. It could provide micro-imaging and micro-medical spectral information simultaneously.

为了在实验上分析人正常胃上皮细胞与癌变细胞的显微后向散射光谱的区别，为临床医学检测提供实验依据，在光纤共聚焦显微成像技术基础上，结合细胞散射理论，建立了一套基于光纤共聚焦的细胞检测显微光谱分析装置，能够同时获取细胞显微图像和显微光谱医学信息。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。