查询词典 cell-free

The invention discloses a connecting city white duck blastodisk fiber cell system with connecting city white duck embryo as material with preservation number at CGMCC No.1877 in cellular biology domain, which is characterized by the following: the fiber cell does not possess epithelial cell with high purity; the quality of freezing cell is stable; the active ratio can reach between 93.5% and 96.8%; the passage growth is stable and fit for big scale culture.

本发明利用连城白鸭胚胎作为材料，进行初代培养、传代培养及细胞冻存等研究。最终获得高活率、高纯度的连城白鸭胚成纤维细胞系，其保藏编号为CGMCC No.1877属于细胞生物学领域。本发明培养的成纤维细胞无上皮细胞等，细胞纯度高；冻存后细胞质量稳定，活率可达到并维持在93.5％～96.8％之间，传代生长稳定，适合大规模培养。

The intralellular labeling methods developed for single cell analysis are summarized in this review, including cell itself acting as a microreaction chamber for derivatization, dericatization mediated by liposome or polyethylene glycol to increase permeabilization of cell membrane, on- and post-column derivatization during single cell analysis with capollary electrophoresis or microchip electrophoresis and labelling of cells with quantum dots.

本文综述了在单细胞分析中常用的荧光标记方法，包括细胞作为微反应器的衍生法，借助于脂质体与聚乙二醇等增加细胞膜通透性的衍生方法和在毛细管/芯片毛细管电泳分析单细胞时柱上衍生和柱后衍生法以及量子点的标记法等。

Mesenchymal stem cells can divide into various cell types, such as ossification cell[13], fat cell[14], cartilage cell[15, 16] etc..

间充质干细胞可分化成多种细胞类型，如成骨细胞[13]，脂肪细胞[14]，软骨细胞[15，16]等。

Epithelial cell defenses include the ciliated epithelial cell s, goblet cell s, and other secretary epithelial cell s of the tracheobronchial tree

表皮细胞的防御包括起纤毛作用的表皮细胞、环状细胞和气管支气管树的其他起分泌作用的表皮细胞。

Morphological conversion from spiralto coccoid forms occurred when HP was cultured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E.coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E.coli,but can not influence activity and gene sequence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果：体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；硒能增强重组VacA对胃癌细胞的抑制作用，使细胞分裂指数和集落形成率降低、S期细胞数量减少，G1期细胞总数增加；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

The students read texts to obtain the microscope-related concepts which included the following: making water mount specimen to be observed by depending on the correct steps, the image of object shown on compound microscope was enlarged directly by the specimen; some differences of structure were found both plant cell and animal's cell, animal's cell does not have cell walls and chloroplasts, it unable to photosynthesis; small living beings of water, consumers, will eat little small living beings.

学生由阅读文章而获得的细胞相关概念主要有：能依正确的步骤，制作一个水埋法标本片进行观察，在复式显微镜下观察到的影像和实物是左右相反、上下颠倒的，植物细胞和动物细胞的形态及构造有些差异，动物细胞不具细胞壁、叶绿体，无法行光合作用，有些水中的微小生物会摄食更小的微小生物，是消费者。

Want to know, the person's skin can be divided into three-layer of cuticular, dermal, subcutaneous tissue about; All maintaining the limits that tastes generation action is in only cuticular layer, in thin thin divided in the cuticular layer that is less than one centimeter 4, quintuplicate cell, be located in the bottommest for base layer, full vivid cell can grow ceaselessly reach dissension, slowly when upgrade is advanced, the cell can squash gradually, at the same time evaporate, nutrient, until most the corneous layer outside, already was flowing do not have the corneous layer cell of life however.

要知道，人的肌肤约可分成表皮、真皮、皮下组织三层；所有保养品产生作用的范围都仅在表皮层，在薄薄不到一公分的表皮层中分了四、五层的细胞，位于最底部为基底层，布满的活细胞会不断生长及分裂、慢慢往上推进时，细胞会渐渐变扁，同时失去水分、养分，直到最外的角质层，就已是平滑却没有生命的角质层细胞了。

The method uses groovy histology and immunity to organize chemical technology, observation mars agent 6- hydroxide via celiac inject sympathetic much cling to small rat small intestine is epithelial after amine the amount of endolymph cell, cup shape cell, IgA+ oar cell, corpulent cell distributings change.

概要： 目的探究交感神经对肠道黏膜屏障的调节功能。方法采用常规组织学和免疫组织化学技术，观察经腹腔注射交感神经损毁剂6-羟多巴胺后小鼠小肠上皮内淋巴细胞、杯状细胞、IgA+浆细胞、肥大细胞的数量分布变化。

Results: It showed that esculin, demethylfuropinarine, decursin and columbianadin inhibited growth of human carcinoma cell line KB cell and their activity showed doseeffect relationship; and umbelliferone renecioate, sphondin and coumestrol inhibited growth of human leukemia cell line HL60 cell and their activity showed doseeffect relationship.

结果：马栗树皮苷、去甲基呋喃皮纳灵、前胡素和二氢山芹醇当归酸酯对人鼻咽癌细胞株KB细胞的生长有一定程度的抑制作用，且呈浓度-效应关系；伞形花内酯刘寄奴酸酯、牛防风素和考迈斯托醇对人白血病细胞株HL-60细胞的生长有一定程度的抑制作用。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。