查询词典 cell-free

Results Of 20 cases, 15 cases were pleomorphic carcinoma(10 cases with adenocarcinoma,3 cases with large cell carcinoma,1 case with squamous cell carcinoma and 1 case with giant cell carcinoma),4 cases were spindle cell carcinoma, and 1 case was giant cell carcin...

结果20例患者中，15例为多形性癌，其中10例伴腺癌成分，3例伴大细胞癌成分，1例伴鳞癌成分，1例伴巨细胞癌成分；4例为梭形细胞癌；1例为巨细胞癌。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

Ornithine decarboxylase, a rate-determining enzyme of the polyamine biosynthetic pathway, is involved in the control of cell cycle, differentiation, cell growth and apoptosis. Previous studies described that protein kinase Cd regulates ODC activity. In this thesis, human leukemia cell line, HL60 cell, was used for the treatment with a specific PKCd inhibitor, rottlerin, to measure the effects on cell growth.

鸟胺酸去羧化（ornithine decarboxylase，ODC）为多胺酸起始与决定反应速率的酵素，对细胞周期、细胞生长、分化及细胞凋亡扮演一重要角色；在寻找肿瘤治疗及预防药物研究中，纯化植物天然物质是其中来源之一，研究者筛选药物时，测定对鸟胺酸去羧化活性是否具抑制效果是一个重要的试验。

In the sub-cluster scale theory, R1 indicated the possibility which the hole skip the middle size hole to become a big cell, it would make striking influence on the tensibility ; R2·R1 indicated the well-distribution degree of cell size, it would make the notable influence on the cell density P; R2/R1 indicated the big cell and small cell exchange possibility, it would make the remarkable influence on the re-spring degree because of the different influence with the hole size.

其中R1表征泡孔越过中等泡向大泡孔缔合的倾向，它对断裂伸长率影响最大；R1·R2表征泡孔的均匀性，受泡孔密度影响较大；lnR2／R1表征体系在发泡过程中大小泡孔相互转化倾向比，由于大小泡孔对泡体回弹率贡献不同，lnR2／R1对发泡体回弹率影响较大。

Conclusions1、E_2 may induce the apoptosis and Fas,FasL expressions of Jurkat cell,its effect positively related to the dose and treating time of E_2.2、There is intrinsic co-expression of ERαand ERβon Jurkat cell,which can be upregulated by E_2,positively related to E_2 doses.3、E_2 may act as an apoptosis inducer of Jurkat cell via ERβ.4、E_2 can promote the transcriptive activity of NF-κB in Jurkat cell.The increased NF-κB activity may lead to increased apoptosis of Jurkat cell.

结论1、E_2可增加Jurkat细胞的凋亡，并增加Fas、FasL的蛋白表达，且其作用与E_2的剂量和处理时间呈正相关。2、Jurkat细胞上存在ERα、ERβ的表达，且E_2可上调这两种受体的表达，E_2剂量越大，ER表达量越高。3、E_2可能通过ERβ发挥其促进Jurkat细胞凋亡的作用。4、E_2能增加Jurkat细胞中NF-κB的转录活性，Jurkat细胞中NF-κB活性增加有促进凋亡的作用。

The area is small to the highest ( conversion efficiency 13 - 15%, some have achieved more than 20%) of monocrystalline silicon solar energy cell conversion efficiency, and output power is big, and stability is good, grows ( generally can reach more than 20 years ) service life, but the raw and processed material cost height, the weak light specific property is wronger, and production technology is complicated, hence the selling price is also higher; The polycrystalline silicon solar energy cell is basic identical with the function characteristic of monocrystalline silicon solar energy cell; The conversion efficiency of non- brilliant silicon solar cell than hangs down ( simples about 6 - 8%), the area is than greatly, but because of his raw and processed material cost than hanging down, can be easy to big area industrialize production, what will have hope to be used the generally big area to generate electricity from now on most will be this kind of application that battery is along with manufacture technique and new material, in a single day the practical package photoelectricity conversion efficiency of its big area achieves more than the 10-15%, and the price of every tile electric power-generating equipment is fallen 1 - 2 U.S.dollar of time, and just is enough to that the solar energy cell efficiency that big area the getting extensive use will constantly replace in now convention certainly special use and the laboratory of sources of energy s will high get many.

单晶硅太阳能电池转换效率最高（转换效率13～15%，有的已达到20%以上），面积小，输出功率大，稳定性好，使用寿命长(一般可达20年以上)，但原材料成本较高，弱光特性较差，生产工艺复杂，故售价也较高；多晶硅太阳能电池与单晶硅太阳能电池的性能特点基本相同；非晶硅太阳能电池的转换效率较低（约6～8%左右），面积较大，但因其原材料成本较低，可便于大面积工业化生产，今后最有希望用于一般大面积发电的将是这种电池。随着制造技术和新材料的应用，一旦它的大面积实用组件光电转换效率达到10-15%以上，每瓦发电设备的价格降到1～2美元时，便足以大面积得到广泛应用并将不断取代现在常规能源。当然特殊用途和实验室中的太阳能电池效率要高得多。

As exposed to optics microscope and inverted microscope,compared to control,SW480 cells with Giemsa staining that treated with the H2RA(cimetidine、nizatidine) took on malignance declining as crimpled firstly,cellular bulk and heteromorphism dimimished,the amount of rounded cell manifolded,nucleolus bulk lessened,dyeing thin,the amount of nucleolus decreased,partial cell organs manifest side-gathering phenomenon on chromatin,and the link between SW480 cell is loosened , subsequently partial rounded cell is floated.When the H2RA(cimetidine 40μM、nizatidine 20μM) against SW480 cells after 3 day,SW480 cells change roundly,nucleolus smashed,shrinking nucleolus came into being,and part of those not adhibited cell wall.

在光镜和倒置相差显微镜下，与对照组相比，经姬姆萨染色观察，西咪替丁、尼扎替丁处理的SW480细胞，先发生皱缩，体积变小，异形性减少，细胞变圆数目增多，细胞核变小，染色变淡，核仁数量减少，部分可见染色质边集现象；细胞间连接疏松，随后部分变圆细胞浮起。40μM西咪替丁及20μM尼扎替丁连续处理3天后，SW480细胞全部变圆，细胞核碎裂，固缩微核形成，部分不再贴壁。

Factors involved in the expression of IFN -αwere studied. These included multiplicity of infection, cell inoculation concertration and cell growth phase. The results show that lower mulitiplicity of infection (M01.4PFU/cell) is beneficial to IFN-α production.The optimal cell inoculation concentration was 〓 cells/flask. The high efficient replication of BmNPV-IFN-α and expression were also due to the proper inoculation time of BmNPV-IFN-α, which was at 48-60 house after cell passage.

研究结果表明，重组病毒感染复数的改变对α-干扰素的产量的影响是时限性的，在一定低感染复数时（Mo1.4PFU），α-干扰素的产量较高；对于重组病毒的复制，最适的细胞接种密度为3.6～7.0×〓细胞/瓶；在细胞指数生长前期（48～60小时）接种重组病毒，对于获得较高的重组病毒效价和α-干扰素的产量都是有利的。

Adding ouiduet epithelial cell or granulose cell to CR1aa or TCM-199 co-culture, the cleavage rate, 4-6 cell developmental rate and 8-16 cell developmental aresignificantly increased (P＜0.05). We can conclude that: CR1aa and TCM-199 containing 5％FBS were the samewith somatic cell cloning in culture in vitro in Yanbian Yellow Cattle.

使用SPSS14.0软件对结果进行方差分析并多重比较后，综合考虑实验结果和方便操作时间与过程，得出以下结论：含5％FBS的CR1aa和TCM-199培养液适用于延边黄牛体细胞克隆胚胎的体外培养。

Penetrating view about scholar , the article inherited the view of many scholars, and increased some of my own opinions, such as point out short information of cell phone concrete handle course " suggestion leader " function strengthen greatly , from " the media is the message " and " media "--The people one extension "watch short information of cell phone the the developments one rationality,"last " weaken reality of function "; In addition, the article has offered the objective standards of how to judge the short information of cell phone in order to be judged of the short information of cell phone through understanding communication of the short information of cell phone, thus know and have certain reference meanings to some mistakes which clarify people.

文章继承了有关学者、专家的精辟观点，并增加了自己的一些见解，如指出了手机短信具体操纵过程中&意见领袖&作用大大加强、从&媒介即讯息&和&媒介——人的延伸&看手机短信发展的合理性、&把关人&作用弱化等现实情况；另外，文章通过对手机短信媒体特点的分析，对澄清人们的一些错误认识也有一定的借鉴意义。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。