查询词典 cell-free

Said cell resorting method includes using sorting label generating module inserting time mark and serial number mark into cell, then transmitting cell from source port to target port; resorting module according to received cell carried time mark and serial number mark resorting cell enqueue information.

该信元重排序方法包括以下步骤：使用排序标签生成模块将时间标记和序号标记插入信元，然后将信元从源端口发送到目的端口；重排序模块根据在目的端口接收到的信元携带的时间标记和序号标记，重排序信元的入队信息。

The experimental results revealed that 60% of cell decay in a nitrifying system was caused by activity decrease and 40% was caused by cell death. In a heterotrophic system, however, activity decrease was responsible for 80% of cell decay, and the other cell decay for 20% was caused by cell death.

结果表明，硝化系统细菌在衰减过程中由细胞活性降低引起的衰减约占60%，由细胞死亡引起的衰减约占40%；而在异养系统中由活性降低引起的衰减和由细胞死亡引起的衰减分别为80%和20%。

It is with printing and section contrast, 124 smear have nuclear cell to decrease in, 58.9% decrease with the 66.1% positive that it is a holiday, common by accident for; of bring down of aplastic anemia, hematopoiesis printing and section cell quantity increase examine disease in, smear still has 26% decrease with the 36% negative that it is a holiday, common disease or conclusion are grow in quantity of red blood cell of lienal function hyperfunction, true sex disease, special the; such as disease of grow in quantity of the plaque that send a gender printing and section cell bring down person in, smear is accorded with rate (91.2% with 97.9%), and in the person that smear cell increases, printing and section are accorded with rate (95% with 100%) all tall.

以印片和切片为对照，124例涂片有核细胞减少中，58.9%和66.1%为假阳性减少，常见误诊疾病为再生障碍性贫血、造血减低；而印片和切片细胞量增加中，涂片仍有26%和36%为假阴性减少，常见疾病或结论为脾功能亢进、真性红细胞增多症、特发性血小板增多症等；印片和切片细胞减低者中，涂片符合率(91.2%和97.9%)，以及涂片细胞增加者中，印片和切片符合率(95%和100%)均高。

Blood bank type the appliance divided by the blood or blood component in the blood bank 滤 to use amid the leuco - cell, and the aetioporphyrin 品 only be limited by sex usage, that product a time the availability to do away with in order not to leuco - cell, object is the leuco - cell the antibody to spark not the 溶 guts have fever the anteiso -, anaphylaxis, HLA the immune response of of same race isoquinoline ketone body and big and small cytidine taken by leuco - cell k virus k, T lymph cell leukemia the k virus k's infection, prevent hard-pressed synthesis 症 of person respiratory trees

血库型应用于血库滤除血液或血液成分中白细胞用，本品只限于一次性使用，该产品有效去除白细胞，目的是为了避免白细胞抗体引起的非溶血性发热反应、过敏反应、HLA同种异体免疫反应以及由白细胞携带的巨细胞病毒、T淋巴细胞白血病病毒的传染，防止成人呼吸窘迫综合症

Methods To investigate the ability of proliferation of the three lung cancer cell lines:the cell line L9981 of human high metastasis lung cancer,the cell line L9981-nm-H1 of transfected nm23-H1 gene,and the cell line L9981-pLXSN of transfected vector using MTT methods,and to examine the ability of extro-invasion of the three cell lines above using improved B...

方法应用放免法检测人高转移大细胞肺癌原代细胞株L9981，转基因细胞株L9981-nm23-H1和空载体转染细胞株L9981-pLXSN的PKA的活性，应用MTT法检测L9981，L9981-nm23-H1和L9981-pLXSN细胞株的体外增殖，改良的Boyden小实法检测L9981，L9981-pLXSN，L9981-nm23-H1这3个细胞株细胞体外侵袭力。

The fern spore, the original cell of the gametophyte, carries out asymmetrical mitosis to produce a large cell and a small cell. The small cell geminates to a rhizoid by tip growth, and the large cell further divides and develops to become a prothallus.

蕨类植物孢子作为配子体的原始细胞，通过不对称的有丝分裂产生一大一小两个细胞，小细胞萌发出极性生长的假根，大细胞继续分裂发育为原叶体。

Results: The coelomocyte could be divided into 7 types: large grained cell, small granular cell, large hyaline leucocyte, small hyaline leucocyte, lanceolate cell, facultative cell and bacillary flutter. The concentration range of the coelomocyte was 5.62~10.00×10^6/ml. In the observation of phagocytize, pseudopod were obviously found and the phagocytic power was strong in the small granular cell.

结果：观察发现罗氏海盘车的体腔细胞可分成：大颗粒细胞、小颗粒细胞、大透明细胞、小透明细胞、柳叶形细胞、兼性大细胞和杆状颤动体；体腔细胞的浓度范围是：5.62~10.00×10^6个／mL。

To investigate the effect of p38MAPK signal conducting pathway on livercancinoma cell's malignant phenotype induced by VEGF,we take theexperiment with cell growth test,scanning microscope and laser scanningconfocal microscope so that observe effect of p38MAPK signal conductingpathway on liver cancinoma cell growth,pseudopodium formation andframework of cytoskeleton induced by VEGF.results indicate that the cellbecame ellipse and there were more and thick pseudopodium in the cell'ssurface after being treated by VEGF,and destroyed framework ofcytoskeleton,which can be blocked by pretreated with a special inhibitor ofp38MAPK SB203580 so that VEGF promote metastasis by enhancing livercell migration and movement via p38MAPK signal conducting pathway,butVEGF promote cell growth without p38MAPK signal conducting pathway.

为进一步探讨VEGF通过p38信号传导通路诱导肝癌细胞转移时，对肝癌细胞恶性表型的影响，采用细胞增殖实验、扫描电子显微镜、激光扫描共聚焦显微镜观察VEGF对肝癌细胞增殖作用、细胞伪足形成、细胞骨架微丝结构的影响以及p38MAPK信号通路调控作用。结果显示，VEGF能够不通过p38信号通路促进肝癌细胞增殖；VEGF诱导肝癌细胞丝状伪足增多、增长，使细胞骨架微丝结构破坏、甚至消失。阻断p38MAPK信号通路，可以抑制VEGF诱导的肝癌细胞伪足形成，细胞骨架丝状结构呈束状，排列较规整。上述结果表明，VEGF可以通过p38信号传导通路诱导肝癌细胞伪足增多、增长，促使细胞骨架微丝结构破坏，使肝癌细胞迁移、运动能力增加，促进肿瘤转移。VEGF并不通过p38信号通路诱导肝癌细胞增殖作用。

Cell lines was established in vitro cell culture system. Only one of it grows well during gene targeting operation. The sex of these cell lines was identified by Sry-PCR.Using lipotransfection method to transfer pZJ into pig fetal fibroblast cell. After 7 days G418 selection, 30 neo-positive cell clones were obtained, among them, 12 of which is non-green ones.

用Sry-PCR法和染色体核型分析法鉴定细胞系的性别，采用优化的脂质体转染法将pZJ转染猪胎儿成纤维细胞，经7天G418(600μg／ml)药物筛选，共获得30个neo阳性细胞克隆，其中12个为非绿色细胞克隆，仅有7个可以扩大培养。

Inaddition,we extract total RNA from cultural U251 cell of humanglioma,make coding gene extron amplification of TfR through ReverseTranscription-Polymerase Chain Reaction,construct expressionplasmid of pCDNA3.1-TfR after digestion of enzyme andconjunction,do transfection to U251 cell by liposome afteridentification,cause excess expression,detect the efficiency of transientexpression of TfR protein by pEGFPN1-TfR, sieve positive cell ofpCDNA3.1-TfR through G418, choose positive cell clone, detect theexpression of TfR in anteroposterior period through Western blot,FCM,cell immunochemistry and mRNA through RT-PCR, identify the effect of transfection.

另外，培养人脑胶质瘤U251细胞，进行细胞总RNA抽提，运用RT-PCR方法进行TfR基因的完整编码区外显子的扩增，经过酶切、连接后构建pCDNA3.1-TfR的表达质粒，鉴定正确后按脂质体转染方法转染人胶质瘤U251细胞，使其过量表达，通过pEGFPN1-TfR检测其瞬时转染的效率；用G418对稳定表达pCDNA3.1-TfR的细胞进行筛选，挑选阳性细胞克隆，运用Western blot、流式细胞检测技术和细胞免疫化学以及mRNA半定量的方法检测TfR在转染前后的表达水平，鉴定转染的效果。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。