查询词典 cell nucleus

Cell lines was established in vitro cell culture system. Only one of it grows well during gene targeting operation. The sex of these cell lines was identified by Sry-PCR.Using lipotransfection method to transfer pZJ into pig fetal fibroblast cell. After 7 days G418 selection, 30 neo-positive cell clones were obtained, among them, 12 of which is non-green ones.

用Sry-PCR法和染色体核型分析法鉴定细胞系的性别，采用优化的脂质体转染法将pZJ转染猪胎儿成纤维细胞，经7天G418(600μg／ml)药物筛选，共获得30个neo阳性细胞克隆，其中12个为非绿色细胞克隆，仅有7个可以扩大培养。

Inaddition,we extract total RNA from cultural U251 cell of humanglioma,make coding gene extron amplification of TfR through ReverseTranscription-Polymerase Chain Reaction,construct expressionplasmid of pCDNA3.1-TfR after digestion of enzyme andconjunction,do transfection to U251 cell by liposome afteridentification,cause excess expression,detect the efficiency of transientexpression of TfR protein by pEGFPN1-TfR, sieve positive cell ofpCDNA3.1-TfR through G418, choose positive cell clone, detect theexpression of TfR in anteroposterior period through Western blot,FCM,cell immunochemistry and mRNA through RT-PCR, identify the effect of transfection.

另外，培养人脑胶质瘤U251细胞，进行细胞总RNA抽提，运用RT-PCR方法进行TfR基因的完整编码区外显子的扩增，经过酶切、连接后构建pCDNA3.1-TfR的表达质粒，鉴定正确后按脂质体转染方法转染人胶质瘤U251细胞，使其过量表达，通过pEGFPN1-TfR检测其瞬时转染的效率；用G418对稳定表达pCDNA3.1-TfR的细胞进行筛选，挑选阳性细胞克隆，运用Western blot、流式细胞检测技术和细胞免疫化学以及mRNA半定量的方法检测TfR在转染前后的表达水平，鉴定转染的效果。

Results The IC50 of SPG-Rg3 was (155.7±0.71) mg·L-1,when the concentration of SPG-Rg3 was arranged from 37.5 to 600.0 mg·L-1,the growth inhibitory rate of MCF-7 cells was higher following the increase of its concentration,the cell growth was greatly inhibited compared with control group(P.05); cell cycle was changed,the cell number of S period increased compared with control group(P.01),the cell number of G2/M period decreased compared with control group(P.01),in experiment group there was an apoptotic apex before G1 apex,and the number of apoptotic cells increased; most of cell nuleus in SPG-Rg3 (150 mg·L-1)experiment group appeared yellow or chrysoidine fluorescence,contracted,beaded and crescent; the result of immunocellularchemical staining of caspase-8 indicated that caspase-8 protein had no expression in control group,but stronger expression in SPG-Rg3 group.

结果：SPG-Rg3 IC50为（155.70±0.71） mg·L-1，当SPG-Rg3浓度在37.5~600.0mg·L-1时，MCF-7细胞的生长抑制率随SPG-Rg3浓度的增加而增大，与对照组比较，细胞增殖受到明显抑制（P.05）；MCF-7细胞的生长周期也发生变化，S期细胞数增加，明显高于对照组（P.01，G2/M期细胞数则明显少于对照组（P.01；并且在G1峰前出现明显的凋亡峰，凋亡细胞数增加。

The relative cell groth rate of L929 cell represented the proliferation of cells on materials with respect to control over time. The result showed that cytotoxicity of thermo-sensitive cell culcure dish was none or little,which can be used for lab use.Additional cell culture was performed with two types of celland cell sheets detachment were observed by reducing the culture tempareture after the confluently growth of the two types of cells.

进一步利用两种不同的细胞永生化的成牙本质细胞系和人牙周膜细胞在接枝有PNIAPm的培养皿上进行培养，观察到细胞可以正常贴壁，生长并增殖，并且在形成连续的细胞层之后，通过降低温度，获得了完整的细胞层结构，结合前两部分实验结果，证实了利用等离子体预处理和紫外线照射接枝制备温敏性培养皿的可行性，为后续研究提供了实验基础。

Results Of 62 cases of PMLGI, 6 cases were low grade malignant B-cell lymphoma of MALT type, 14 cases high grade malignant B-cell lymphoma of MALT type with a low grade malignant component, 1 case lymphomatous polyposes, 2 cases Butkitt-like lymphoma, 38 cases B-cell large cell lymphoma and 1 case intestinal T-cell lymphoma.

结果：62例PMLGI中，低度恶性B细胞粘膜相关组织型淋巴瘤6例，高度恶性B细胞粘膜相关组织型淋巴瘤伴低恶成分14例，淋巴瘤性息肉病1例，Burkitt样淋巴瘤2例，B大细胞淋巴瘤38例，肠道T细胞淋巴瘤1例。

Clear cell carcinomas that may occur in the lower urinary tract include variants of the more common cancers such as prostatic adenocarcinoma and transitional cell carcinoma as well as infrequent carcinomas such as clear cell carcinoma, which is similar to that of mullerian origin and metastatic renal cell carcinoma.8 A benign process that occasionally has been reported to show marked clear cell change is nephrogenic adenoma, a lesion that is considered a benign metaplastic process.3,5,12 Perhaps because of the great histo

透明细胞癌可能发生在较低泌尿系统包括变异更常见的癌症如前列腺腺癌和过渡细胞癌以及罕见的癌如透明细胞癌，它是相似的苗勒管起源和转移性肾细胞癌 18亿美元的良性碾压工艺，偶尔被报告显示标记为透明细胞变化腺瘤，被视为是一种良性病变的化生 process.3，5，12也许因为伟大的历史

And the conditions in cell suspension culture of Mikania micrantha were studied. The results showed that sucrose was the compatible carbon sucrose, and 30g/L sucrose concentration can satisfy the growth of Mikania micrantha cell; ammonium was absorbed under different sucrose concentration that haven't demonstrated significant specificity, and was completely absorbed on the lag phase and the early logarithmic phase; while nitrate was mainly absorbed on logarithmic phase. The density-dependent of Mikania micrantha cell starting to grow and density-inhibited of cell growth were proposed, the fittest inoculating quantity of Mikania micrantha in cell suspension culture was 40g/L.

并对微甘菊细胞悬浮培养条件进行研究，结果表明，微甘菊细胞生长适宜的碳源是蔗糖，并且较合适的蔗糖浓度为30g／L；在不同蔗糖浓度下氨基氮的吸收差别不大，氨基氮在迟滞期和对数期前期已基本消耗完毕，对数期生长主要利用硝基氮，因而提出，在微甘菊细胞液体悬浮培养中，氮源可以采用初始低氨基氮浓度，对数期中间维持高硝基氮，而且对数期中逐步流加少量的氨基氮；微甘菊细胞迟滞期分裂启动存在密度依赖现象，对数期生长存在密度抑制现象，最适接种量为40g／L。

Results］ There were 11 cases with clear cell carcinoma; 1 case, granular cell carcinoma; 4 cases, mixed cell carcinoma; 1 case, multilocular cell carcinoma; 1 case, papillary renal carcinoma; 2 cases, chromophobe cell carcinoma and 1 case, collecting duct carcinoma.

结果］ 11例透明细胞癌，1例颗粒细胞癌，4例混合细胞癌，1例多房囊性肾细胞癌，1例乳头状癌，2例嫌色细胞癌，1例集合管癌。

Western Blot indicated that AIF was selectively released before cytochrome C from the intermembrane space of mitochondria to cytosol, and that release needed Bax relocation to mitochondria. Results above suggest that caspase-independent cell death maybe occurred earlier than caspase-dependent cell death in staurosporine induced T lymphocytes apoptosis. Caspase-independent cell death is an alternative pathway of cell death which could guarantee the safe and noninflammatory removal of corpses when caspase was mutented or blocked in the cell, it plays an important role in maintaining the number of T lymphocytes and ensuring the proper function of immune system.

细胞caspase非依赖死亡方式可能是Caspase依赖性死亡方式的替代途径，它可以保证在Caspase发生突变或通路受阻时，T细胞仍然能够正常死亡，这对于维持T细胞的数量和保证免疫系统正常功能的发挥具有十分重要的意义。

Results Of 20 cases, 15 cases were pleomorphic carcinoma(10 cases with adenocarcinoma,3 cases with large cell carcinoma,1 case with squamous cell carcinoma and 1 case with giant cell carcinoma),4 cases were spindle cell carcinoma, and 1 case was giant cell carcin...

结果20例患者中，15例为多形性癌，其中10例伴腺癌成分，3例伴大细胞癌成分，1例伴鳞癌成分，1例伴巨细胞癌成分；4例为梭形细胞癌；1例为巨细胞癌。

"Bird Man's Diary, Butting on the Love Island", Changsha, China

2008年"鸟人日记，顶在爱情岛"，中国长沙

As long as they were in the service of a family of wizards, their magic would remain dormant.

只要他们一天为家庭服务，他们的法力就潜藏不动。