查询词典 cell cleavage

Different strains have different reaction to the delay of oviducts in body after animal death. After 10min delay C57BL/6 mouse oocytes have death rate of 56.5%,significantely higher than Kunming mouse (47.6%);Spontaneous activation rates were respectively 13.3% and 46.0%, C57BL/6 were obviously lower than Kunming mouse.4. The oviducts were obtained after being delayed 5min 24h after the mice were injected with hCG. The oocytes were cultured in CZB. About 81.1% occurred spontaneous activation, evidently lower than parthenogenetic rate (96.4%) with SrCl_2. Spontaneous activable oocytes had high cleavage rate(93.2%) and 4-cell rate(87.3%). However, spontaneous activable oocytes had blastula development rate(18.7%) as low as parthenogenetic oocytes by SrCl_2(22.9%).

不同品系小鼠卵母细胞对输卵管在体内滞留产生的反应不同，滞留10min C57BL/6系小鼠卵母细胞死亡率为56.5%，显著高于昆明鼠(47.6%)；自发激活率分别为13.3%和46.0%，C57BL/6系小鼠显著低于昆明鼠。4.hCG后24h体内滞留5min卵母细胞在CZB中培养自发激活率为81.1%，显著低于SrCl_2孤雌激活率(96.4%)；自发激活的卵母细胞有较高的卵裂率(93.2%)和4-cell比率(87.3%)，但囊胚率(18.7%)较低，同卵龄的卵母细胞经SrCl_2孤雌激活囊胚发育率为22.9%，差异不显著。

Its biological characteristic express two ways: increase wall of micrangium"s permeability and conjugate vascular endothelial cell"s specificreceptor, anxo-act vascular endothelial cell cleavage and proliferation, so lead to formate neogenetic vascular.

VEGF的生物学特性主要表现在两方面：增加微血管的通透性和特异性的与血管内皮细胞受体结合，促进血管内皮细胞的分裂和增殖，进而导致新生血管的生成。

The cleavage rate of ovocyte IVF of MⅡovocyte frozen withOPS method and activation method was higher remarkably than fluid spearmethod(P＜0.01), the cleavage rate of 2-cell after electrical activation was higher than IVF.

本试验推荐猪体外成熟培养体系为连续培养液48h的NCSU-23+10％FBS，细胞浓度为100个／400μl，发育培养体系为NCSU-23+BSA；用CRY-3细胞激活仪电激活时(电融槽宽度为1mm)为150v／20μs／2次+600μMγ-BLI；以活力为0.1左右的冷冻精液进行IVF时，精子浓度为10~7，共孵时间为6h，并在成熟处理时只部分去除卵丘细胞；MⅡ卵母细胞冷冻时，方法为移液枪头法+离心法，冷冻后激活的2-细胞卵裂率比IVF高。

Cell proliferation was measured with cell number,cell metabolic activity (WST-1 cleavage),DNA synthesis and cell cycle assayed with flow cytometry.

进行新西兰白兔心脏心肌成纤维细胞的培养；测定细胞计数，细胞活性（WST－1分解）和DNA合成，并以流式细胞仪测定细胞周期等以表示细胞增殖。

Methods: Cardiac myofibroblasts derived from heart of New Zealand rabbit were cultured and identified. Cell proliferation was measured with cell number,cell metabolic activity (WST-1 cleavage),DNA synthesis and cell cycle assayed with flow cytometry.

进行新西兰白兔心脏心肌成纤维细胞的培养；测定细胞计数，细胞活性（WST－1分解）和DNA合成，并以流式细胞仪测定细胞周期等以表示细胞增殖。

The average cell number of each embryo were significant differences between 12-24 hr and 36-48 hr treatment and 0-72 hr treatment of early blastula; the cleavage index of each embryo were significant differences between 0-12hr and 24-36 hr treatment and 0-72 hr treatment of the hatching of blastula; embryo cell number and cleavage of other treatments were no differences.

早期囊胚的12-24h处理组和36-48h处理组的每一胚胎平均细胞数与0-72h处理组有显著差异；扩张囊胚0-72h处理组的分裂指数与0-12h处理组和24-36h处理组有显著差异，其它各组的胚细胞数和分裂指数均无差异。

At present time people has found that various oxidants exist in injured cells, including peroxide and other free radical (eg: peroxide anion and hydroxide radical ion); these ROS also exist in abnormal cells arose from cell cleavage block and cell function loss.

目前已经发现，损伤的细胞中含有较多的各类氧化物，包括过氧化氢以及其它自由基(例如过氧化物阴离子和氢氧根离子)；在因细胞分裂阻断以及细胞功能丧失所引起的异常细胞中也含有这些活性氧类物质。

We biopsied 1-2 single blastomere from 6-8 cell cleavage stage intracytoplasmic sperm injection embryo, and using nested PCR amplified the high frequently mutation region G380R of FGFR3 gene of the single blastomere. The products of PCR were digested by restriction enzyme Bfm Ⅰ, then the digested products were detected by 10% PAGE to see whether the embryo inherted the mutation of the patient and to screen out normal embryo transfer.

活检经胞质内单精子注射(intracytoplasmic sperm injection，ICSI)授精的胚胎发育至6～8细胞期的1～2个单卵裂球，采用巢式PCR扩增单卵裂球的FGFR3基因的高发突变位点G380R区域，用限制性内切酶BfmⅠ消化巢式PCR的内扩增产物，再经10%聚丙烯酰胺凝胶电泳检测有无遗传患者的该种突变，从而筛选出正常胚胎移植。

Fluorescence staining of cell framework and nuclei were conducted, the result of which revealed that the transfected cells displayed disorder in cell skeleton, shrinkage and cleavage in nuclei. It was illuminated that chromosome DNA occurred cleavage and fragment since the result of TUNEL detection was positive. The transfected cells also displayed more typical apoptotic character, including exposure of phosphatidylserine in the plasma membrane by Annexin V staining detection, shrinkage nuclei, chromatin condensation, plasma membrane blebbing and chromatin breakage fragments by electron microscopy analyses.

细胞骨架和细胞核染色结果表明，AIF基因转染细胞呈现细胞骨架的紊乱、细胞核固缩、分裂等变化；TUNEL检测呈阳性，说明有染色体DNA出现断裂；Annexin V染色检测到转染细胞出现胞膜磷脂酰丝氨酸的暴露；电镜观察的结果显示，转染目的基因组细胞呈现细胞膜出泡、细胞核固缩、染色体凝集和断裂等典型的凋亡细胞特征。

Adding ouiduet epithelial cell or granulose cell to CR1aa or TCM-199 co-culture, the cleavage rate, 4-6 cell developmental rate and 8-16 cell developmental aresignificantly increased (P＜0.05). We can conclude that: CR1aa and TCM-199 containing 5％FBS were the samewith somatic cell cloning in culture in vitro in Yanbian Yellow Cattle.

使用SPSS14.0软件对结果进行方差分析并多重比较后，综合考虑实验结果和方便操作时间与过程，得出以下结论：含5％FBS的CR1aa和TCM-199培养液适用于延边黄牛体细胞克隆胚胎的体外培养。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失