查询词典 cascade amplification

There were 8 embryos single blastomere amplified, and the amplification success rate was 72. 7%(8/11); 3 embryos' not amplified; 2 embryos'showed preferential amplification , and the PA rate was 25%(2/8); and the 7 tubes of blank with washing cell solution were not contaminated. We transferred 3 unaffected embryos to the uterus. But unfortunately, blood hCG was negative after 2 weeks of transferring indicating no pregnancy.

共8枚胚胎的单卵裂球得到扩增，扩增成功率为72.7%（8/11），3枚未得到扩增，2枚发生了偏性扩增(preferentialamplification，PA)，PA的发生率为25%（2/8），细胞洗脱液空白对照7管均无污染发生。3枚诊断为正常的胚胎被移植入宫内，不幸的是，移植后2周，血hCG阴性，证明未妊娠。

DNA were abstracted by means of phenol chloroform, amplification and cataphoresis test of polymerase chain reaction of target DNA,primer sequence of gene segment amplification of 5-hydroxytryptamine (5-HT) 2A receptor gene T102C: 5'-TCT GCT ACA AGT TCT GGC TT-3', 5 '-CTG CAG CTT TTT CTC TAG GG-3'; Reaction system of 50 μL, DNA 0.5 μg,Primer 50 pmol,TagDNA enzyme 2U were added with dNTP to the final concentration of 200 μmol/L.

应用常规苯酚氯仿法抽提DNA，目的DNA的聚合酶链反应扩增及电泳检测，5-羟色胺2A受体基因T102C基因片断扩增的引物序列：5'-TCT GCT ACA AGT TCT GGC TT-3'。5'-CTG CAG CTY TTT CTC TAGGG-3'；采用50 μL反应体系，DNA 0.5 μg，引物50 pmol，TagDNA酶2U加dNTP至终浓度200μmol/L。

The section of voltage amplification is realized by the integration operational amplifier and the current amplification section is made up of the VMOS.

电压放大级采用集成放大器实现，电流扩展级则采用功率MOS管组成互补输出电路。

All 5 cases of DDL showed MDM2 and/or CDK4 amplification (100%), whereas 6 of 45 UHGPSs showed MDM2 and/or CDK4 amplification (13%).

所有5例DDLs显示MDM2和/或CDK4基因扩增（100%），而45例UHGPSs中有6例显示MDM2和/或CDK4基因扩增（13%）。

In this model,the molecular basis that PCR reaction is doomed to change from exponential amplification to linear amplification had been clarified.

本模型阐明了PCR反应在多次循环后必然由指数扩增转变为线性扩增的分子基础，为定量PCR 提供了准确的计算方法。

After that, we detect cell DNA heteroploid and cell cycle transformation of human osteosarcoma tissue by using flow cytometry method; next, we amplification the exon2 and exon3 of 〓 gene in 36 cases human osteosarcoma tissue, and amplification products were performed the sepharose and polyacrylamide gel electrophoresis immediately.

然后采用原位杂交方法，对45例骨肉瘤标本组织和10例骨纤维结构不良组织中〓基因mRNA进行检测，以了解骨肉瘤标本组织和骨纤维结构不良组织中〓基因在蛋白质水平和mRNA水平的表达情况。

A method for detecting the presence of a target nucleic acid sequence in a sample, said method comprising subjecting said sample to an amplification reaction using a set of nucleotides, at least one of which is fluorescently labelled, contacting amplification product with a probe under conditions in which the probe will hybridise to said target sequence, said probe comprising a reactive molecule which is able to absorb fluorescence from or donate fluorescent energy to said fluorescent labelled nucleotide and monitoring fluorescence of said sample.

一种用于检测在某样品中是否存在目标核酸序列的方法，该方法包括：使用一套核苷酸对所说的样品进行扩增反应，其中至少一个核苷酸是被荧光标记的，在探针与目标序列杂交的条件下使扩增产物与探针混合，所说的探针包括一种活性分子，该活性分子能够从荧光标记核苷酸吸收荧光或向其贡献荧光能量，及检测样品的荧光。

22 Of 22 pairs EST-SSR primers from Chinese cabbage/rape respectively had amplification products in soybean,wheat and maize,the rate of amplification was 81.8%,95.4%,100%,respectively;10 of 22 pairs EST-SSR primers showed polymorphism in monocotyledons wheat and maize,13 in dicotyledons oilseed rape and soybean,7 in both monocotyledonous and dicotyledonous crops.

（1）30对小麦EST-SSR引物中，有29，28和26对引物分别在玉米、油菜和大豆中有扩增产物，可扩增率分别为96.7%，93.3%和86.7%；其中分别有12对和9对引物在单子叶作物小麦和玉米及双子叶作物大豆和油菜中的扩增产物显示多态性，有4对引物在4种作物中的扩增产物均显示多态性。

(1)29,28,26 of 30 pairs of EST-SSR primers from wheat respectively had amplification products in maize,oilseed rape and soybean,the rate of amplification was 96.7%,93.3%,86.7%,respectively;12 of 30 pairs EST-SSR primers from wheat showed polymorphism in monocotyledons wheat and maize,9 in dicotyledons oilseed rape and soybean,and 4 in both monocotyledonous and dicotyledonous crops.

基金项目：国家生命科学与技术人才培养基地科技创新基金项目(2007088)；国家油菜产业化项目科学家岗位"化杀岗位"（nycytx-00505）；国家自然科学基金项目(30871578)中文摘要：探讨单、双子叶作物间EST-SSR引物和标记的通用性。选取30对小麦、8对油菜和14对白菜的EST-SSR引物，分别以10个小麦、10个油菜、5个大豆品种和5个玉米自交系的基因组DNA为模板，进行PCR扩增及扩增产物的多态性分析。

The method is based on real time reverse-transcription-PCR amplification of mRNA to detect viable Listeria monocytogenes by specific hlyA gene. This method can overcome the false-positive caused by amplification of nonviable Listeria monocytogenes in the detection process.

为克服传统PCR检测的假阳性问题，有效的检测单增李斯特活菌，本研究提取单增李斯特菌的总RNA并进行反转录，以单增李斯特菌的必要毒力基因hlyA为靶基因，设计特异性引物和TaqMan探针进行实时PCR检测，研究了检测的特异性和灵敏度，考查了对人工污染牛乳进行检测的应用性。

According to our present broadcasting situation,the thesis proposes using new technology to update and reform the present HFC. It also suggests broadcasting using self-advantage and arranging EPON in order to win the supermarket.

论文从我国广电的现状出发，结合实际，积极倡导采用新技术更新、改造我国现有的HFC网络；倡导广电现阶段利用自身优势，部署EPON网络来赢取市场。

A new general kinetic equation was derived in which the effect of the removal of condensation water was taken into account completely, and was applied to the polyesterification kinetic study of AA/HPHP and AA/NPG. It was found that the reaction order of self-catalyzed polyesterification was not constant. For these polyesterifications, the reaction order was 2.5 at a low temperature, while 3.0 at a high temperature.

考虑缩合水的排除对反应体系的影响，用体积浓度单位推导出一个新的聚酯反应总包动力学方程，并应用到AA/HPHP和AA/NPG两个聚酯化反应的动力学研究中，研究发现，自催化聚酯反应的反应级数并不是固定值，对于AA/HPHP和AA/NPG两个聚酯化反应而言，低温时反应级数为2.5，高温时反应级数为3.0。