查询词典 cascade amplification

PCR amplification of di-, tetra- and hexaploid materials using genome-specific primers indicated that the NUMT inserted in the nucleus when the tetraploid wheat speciated or after that time and retained until the hexaploid wheat speciated.

用基因组特异引物扩增二倍体、四倍体和六倍体小麦，结果表明该插入事件发生在四倍体小麦形成时或形成后，并且一直保留到六倍体。

The QL series hydraulic splitter is our newly developed product which is composed of two parts:hydraulic pumping station and splitter. The super-high-pressure oil-driven oil cylinder outputted by the pumping station generates gigantic driving power, which can split the object to be split to the preset direction after mechanic amplification.

QL-P38系列液压劈裂机是我们研制开发的新产品，它由液压泵站和分裂器两大部分组成，由泵站输出的超高压油驱动油缸产生巨大推动力，并经机械放大后即可使被分裂物体按预定方向裂开。

The numerical results show that the amplification of optical solitons is unstable in the anomalous dispersion regime.

数值计算结果表明：在反常色散范围内，光孤子的放大是不稳定的。

The real-time PCR results show that 10 isolates were amplified while 54 isolates did not produce considerable amplification. So that , the 10 isolates were identified as E coli O157 and the 54 isolates were non- E coli O157, which comply with the results of serotyping.

说明出现扩增曲线的10株细菌是大肠杆菌0157，未出现指数扩增的54株细菌是非0157大肠杆菌，荧光定量PCR法和血清学检测结果完全一致。

The results of microsatellite amplification showed that (1) the genetic relationship is closer among the same body colour bio-type individuals of green peach aphid than that between the red and the green bio-type individuals from three kinds of host plants.

微卫星扩增结果表明：(1)来自不同寄主植物上的同一体色生物型个体之间的遗传关系近于不同体色生物型个体之间的遗传关系。

Then, the amplitude amplification for the pile cap response of the coupled system to the bedrock shaking was obtained, and the dynamic interaction was reflected.

对耦合体系桩承台对基岩运动的放大效应进行了求解，此放大效应反映了耦合体系的动力相互作用。

After removing the plane-parallel cavity, the seed pulse energy is about 8 mJ from the grating cavity and the energy is boosted to 60mJ with additional double pass amplification and extender telescrope.

实验获得了8 mJ的主振荡器激光能量输出，经过双程放大和望远镜扩束后输出激光能量达60 mJ，线宽为0.6 cm-1，光束发散角接近衍射极限，满足了后续放大需要。

The results showed PERV proviral DNA and mRNA could be detected in all 31 individuals by PCR and RT-PCR, respectively. env-A, env-B and env-C were only detected in 2 individuals, while in the other 29 individuals, only env-A and env-B but not env-C was detected. The quantity of DNA in PCR amplification of PERV genes should be more than 15 ng.

PCR和RT-PCR结果表明，所检测的31头沙子岭猪均带有PERV前病毒DNA，耳样组织中均有PERV mRNA表达，其中有2头个体携带 env-A、env-B、env-C 3种囊膜蛋白基因，而其余的29头个体只带有env-A、env-B 两种囊膜蛋白基因，未检测到env-C基因。

The recombinant fowl poxvirus rFPV-E0-E2 was serially passaged for 20 passages in specific-pathogen-free chicken embryo fibroblast culture. The passage 5, 10, 15 and 20 were chosen to identify by blue plaque assay, PCR amplification, gene sequence analysis and indirect immunofluoresence assay.

将重组鸡痘病毒rFPV-E0-E2在鸡胚成纤维细胞上连续传20代，取第5、10、15和20代重组病毒进行以下检测：用蓝斑试验鉴定各代重组病毒纯度；用PCR方法扩增猪瘟病毒E0、E2基因，进行基因序列分析；用间接免疫荧光实验检测各代重组病毒中猪瘟病毒E0、E2基因的表达。

ACGM-PCR amplification from 12 Signal grass germ plasms, 126 bands are obtained, 30 primers of the 37 primers can obtain specific PCR product from not less than one germ plasm, and 27 primers obtain polymorphism product.

对12份臂形草属种质进行ACGM-PCR扩增，共产生126条谱带，37对引物中，有30对引物可以在至少1份材料中得到特异性PCR产物。

According to our present broadcasting situation,the thesis proposes using new technology to update and reform the present HFC. It also suggests broadcasting using self-advantage and arranging EPON in order to win the supermarket.

论文从我国广电的现状出发，结合实际，积极倡导采用新技术更新、改造我国现有的HFC网络；倡导广电现阶段利用自身优势，部署EPON网络来赢取市场。

A new general kinetic equation was derived in which the effect of the removal of condensation water was taken into account completely, and was applied to the polyesterification kinetic study of AA/HPHP and AA/NPG. It was found that the reaction order of self-catalyzed polyesterification was not constant. For these polyesterifications, the reaction order was 2.5 at a low temperature, while 3.0 at a high temperature.

考虑缩合水的排除对反应体系的影响，用体积浓度单位推导出一个新的聚酯反应总包动力学方程，并应用到AA/HPHP和AA/NPG两个聚酯化反应的动力学研究中，研究发现，自催化聚酯反应的反应级数并不是固定值，对于AA/HPHP和AA/NPG两个聚酯化反应而言，低温时反应级数为2.5，高温时反应级数为3.0。