查询词典 caprine

Then the caprine fetal fibroblast cells were transfected with p6.5hLF-EGFP by LipofectamineTM-2000 and selected by G418 for 3 to 4 weeks. The G418 resistant transfectants were identified by PCR and EGFP detection. The results indicated that the transgene was stably integrated into the open region of the chromatin of G418 resistant fibroblast cells.

脂质体介导法转染山羊胎儿成纤维细胞，G418抗性筛选3-4周后，经PCR扩增和报告基因EGFP表达检测，得到稳定整合外源基因的转基因供体细胞系，为制备高效表达人乳铁蛋白的转基因山羊乳腺生物反应器提高可靠的核移植供体细胞。

The technological conditions were optimized by a response surface experiment. The proportion of embedding agent and caprine oil is 1:1.11; the water content in emulsion is 81.2%; the homogenous pressure is 25.7 MPa.

通过响应面试验，确定羊油脂粉末工艺条件为：包埋剂与羊油比例为1：1.11；乳状液中含水量为81.2%；均质压力为25.7 MPa。

The caprine IL-2 gene and the IFN-γgene without the signal peptidesequence were subcloned into (5") Bam H I and (3") HindⅢrestriction sites of pET30aplasmid, the recombinant plasmid were transformed into E. coli BL21(DE3) and induced withIPTG, they were demonstrated by SDS-PAGE that the genes were expressed as inclusion bodies.

应用DNA重组技术，将IL-2基因和去除信号肽的IFN-γ基因分别插入到原核表达载体pET-30a的BamHⅠ和HindⅢ位点上，构建原核表达质粒pET30a-CapIL-2和pET30a-CapIFN-γ，转化大肠杆菌BL21(DE3)，经IPTG诱导后进行SDS-PAGE电泳，结果表明目的基因在大肠杆菌中以包涵体形式融合表达。

Heat inactivation or forced weakness of the M.paratuberculosis to immunize the cattle and caprine has been comployed. This method can depress theincidence rate. However, the serum becomes positive after vaccine inoculation.

此外，国内外也有采用预防接种的方法来控制该病，即将M.paratuberculosis热灭活或致弱后制成疫苗，免疫接种牛和羊，可降低该病的发生率。

MethodsThe distilled bovine and caprine placental peptide s were prepared by ultrafiltering and their relative molecular weights were mensurated by improved Tricine-SDS-PAGE eletrophoresis with urea.

结果从牛和羊胎盘中成功提取出了胎盘肽，其相对分子质量分别为4 785和4 386；其最大吸收峰分别为210.5和225.4 nm；牛和羊胎盘肽均可使顺铂抑制的兔外周血T淋巴细胞的转化率显著提高，其中牛胎盘肽以1∶1×103、1∶1×1042组吸收度明显高于抑制组(P.05)；羊胎盘肽以1∶1×102、1∶1×103和1∶1×1043组效果最为明显(P.01)。

In addition, this bacterial stain could also degraded a-keratins which include hard keratins (cattle horn、caprine horn、human finger nail、Bauhinia variegata) and soft keratins (wool、human hair、cattle hair).

另外，此菌也可降解a角蛋白，包括硬质角蛋白(牛角，羊角，指甲，羊蹄角)和羊毛、头发、牛毛等软质角蛋白。

1Livestock Research Institute, Council of Agriculture(2)Department of Animal Science, National Pingtung University of Science and TechnologyGenetic variation in the N-acetylglucosamine 6-sulfatase (G6S) gene is the key role in caprine Mucopolysaccharidosis IIID.

本研究建立山羊黏多醣症遗传缺陷之单股构型多态性基因型检测方法，应用此方法来检测乙醯醣胺氨基硫酸酶(N-acetylglucosamine 6-sulfatase， G6S)基因型不再需要使用限制酶，可节省检测时间、成本与人力。G6S基因的遗传变异在山羊黏多醣症第三型扮演重要的角色。

The new GV preparation method we developed allowed the best visualization of chromatin distribution after Hoechst or CMA3 staining. Our results showed that GV chromatin configurations are different among different species, and thus, while chromatin condensed into a ring around the nucleolus in porcine and bovine oocytes, no such a ring was observed in caprine oocytes; Porcine oocytes were synchronized at GV1, goat oocytes at GV3n and bovine oocytes at GVf configurations before GVBD occurred, indicating that these configurations are progressive towards final maturation rather than atresia; Changes of chromatin configurations were associated with gene activity of transcription; GV chromatin configurations were affected by the environmental changes such as serum starvation and elevated temperature.

应用该技术对猪、山羊和牛不同大小、不同健康程度卵泡及体内和体外成熟过程中卵母细胞染色质构型的变化发现：1）不同动物卵母细胞染色质构型不同；猪和牛卵母细胞染色质围绕核仁凝集形成环，但山羊卵则没有此环。2）卵母细胞在GVBD之前都同步在某一染色质构型；猪同步在GV1，山羊GV3n，牛GVf，说明这些染色质构型代表着进行性变化。3）染色质构型的变化反映基因转录活动的变化。4）染色质构型受环境变化的影响。

The cDNA open reading frame of IFN-γis 501bp,encoding a putative 166 amino acid protein (19.327KD), the DNA sepuence homology of thiscaprine IFN-γand the corresponding caprine(U34232) cytokine is 99.4％, SignalP 3.0 analysisdemonstrated that the first 23 amino terminal aa sequence compose a hydrophobic signal sequence.

测序结果显示克隆的IFN-γ基因全长501个核苷酸，编码166个氨基酸，该基因与GenBank发表的山羊干扰素-γ基因序列(U34232)比较，核苷酸／氨基酸序列同源性为99.4％。

You can buy sweetened beverages, if that suits you.

你若喜欢的话，可以买些含有糖精的混合饮料。

As many of you are probably aware, Nick and Vanessa were recently faced with an embarrasing sex scandal after naked pictures of the two getting jiggy with it in a hot tub surfaced the net.

正如很多你可能知道，尼克和 Vanessa 最近面临一个尴尬的绯闻事件后，赤裸裸的图片两个越来越jiggy与它在浴缸中的一个热点浮现净。