查询词典 can't

Focus on what you can control, and don't stress so much about what you can't.

专注于你能够控制的事情，不要对于那些你无法控制的事情给自己太大的压力。

I can't tell you to remember me, but I can't bear for you to forger me.

我没资格告诉你让你记住我，但是我无法忍受你会忘掉我。

Even though you don't write in that manner very often, is there anything you admire that free verse can do that maybe meter and rhyme can't?

尽管你不常用这种方式写诗，我想问自由诗有没有为你所欣赏的、步格和韵格可能不具备的优点？

We needed that to be a full shot , so I said,"If we can't do it in one go, we can't do it."

我们想要一个全景镜头，所以我说，"如果我们不能一个镜头拍成，我们就不要拍了。"

Gabby: If I can't trust you, I don't think I can be with you.

如果我无法相信你了，我们也就不必在一起。

The two protagonists of M. Butterfly have complex psychology. Song Liling is entangled in love and hatred and can\'t extricate herself. She becomes a tragical character in moral. Gallimard can\'t tolerate the conflict between illusion and reality and get suicide finally.

蝴蝶君》中的两位主角的心理是极为复杂矛盾的：宋丽玲在爱与恨的纠结之中不可自拔，走向道德意义上的悲剧；伽利玛无法接受幻想和现实的冲突，走向自杀的悲剧。

Chris Gardner:People can't do something by themselves; they wanna tell you you can't do it.

当人们做不到一些事情的时候，他们就会对你说你也同样不能。

In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

"You can't buy glamour, and you can't fake it."

"魅力是买不到、也装不出来的。"

With an upright torso, you can't use the gluteus muscles to good effect. Also, you can't effectively pull up on the handlebar from an upright position.

如果你挺直身体，那你的肌肉就不能很好的运动，不能使你有效的把车子停住。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。