查询词典 can be used

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

The research indicated that,the 36°Bè xylose mother liquor was used as material,9.60% catalyst M-16 was added,and after caramelized 180min at 120℃,a caramel...

研究表明，在36°Be′的木糖母液中，加入9.60%的M-16催化剂，于120℃下焦糖化反应180min，得到色率为2.8万EBC单位，红色指数达6.3以上的焦糖色素。

Two merging methods of Bézier curves are generalized to tensor product Bé zier surfaces, we propose two methods to approximate two pieces of adjacent tensor product Bézier surfaces by a piece of tensor product Bézier surface. We offer the explicit formulas of the merged tensor product Bézier surfaces with all kinds of merging conditions.

同样，Bézier曲线的两种合并方法也被成功的推广到张量积Bézier曲面上，得到把两片相邻张量积Bézier曲面合并成一片张量积Bézier曲面的两种合并方法，并给出在各种合并条件下的合并曲面控制顶点的显示表示式。

I can ride my bike with no handlebars No handlebars No handlebars I can ride my bike with no handlebars No handlebars No handlebars Look at me, look at me hands in the air like it's good to be ALIVE and I'm a famous rapper even when the paths're all crookedy I can show you how to do-si-do I can show you how to scratch a record I can take apart the remote control And I can almost put it back together I can tie a knot in a cherry stem I can tell you about Leif Ericson I know all the words to "De Colores" And "I'm Proud to be an American" Me and my friend saw a platypus Me and my friend made a comic book And guess how long it took I can do anything that I want cuz, look: I can keep rhythm with no metronome No metronome No metronome And I can see your face on the telephone On the telephone On the telephone Look at me Look at me Just called to say that it's good to be ALIVE In such a small world I'm all curled up with a book to read I can make money open up a thrift store I can make a living off a magazine I can design an engine sixty four Miles to a gallon of gasoline I can make new antibiotics I can make computers survive aquatic conditions I know how to run a business I can make you wanna buy a product Movers shakers and producers Me and my friends understand the future I see the strings that control the systems I can do anything with no assistance Cuz I can lead a nation with a microphone With a microphone With a microphone And I can split the atom of a molecule Of a molecule Of a molecule Look at me Look at me Driving and I won't stop And it feels so good to be Alive and on top My reach is global My tower secure My cause is noble My power is pure I can hand out a million vaccinations Or let'em all die in exasperation Have'em all healed of their lacerations Have'em all killed by assassination I can make anybody go to prison Just because I don't like'em and I can do anything with no permission I have it all under my command Because I can guide a missile by satellite By satellite By satellite And I can hit a target through a telescope Through a telescope Through a telescope And I can end the planet in a holocaust In a holocaust In a holocaust In a holocaust In a holocaust In a holocaust I can ride my bike with no handlebars No handlebars No handlebars I can ride my bike with no handlebars No handlebars

我可以坐我的自行车没有把手没有把手没有把手我可以坐我的自行车没有把手没有把手没有把手看我，看我手在空气中一样，它的良好的可活着和我是一个著名的说唱甚至当paths're所有crookedy 我可以告诉您如何做硅做我可以告诉您如何从头纪录我可以采取除了远程控制我几乎可以把它连同我可以配合一结在一个樱桃干我可以告诉你，约莱夫帕格ericson 我知道的所有字眼，以"德colores "和"我很自豪地成为美国"我和我的朋友看到一个鸭嘴兽我和我的朋友作出了漫画书和猜测多久才我可以做任何事情，我想cuz ，请看：我可以保持节奏，没有节拍器没有节拍器没有节拍器我可以看到你的脸就电话关于电话关于电话看我看我只是所谓的说，它的良好的可活着在这样的一个小世界我所有的卷曲与一本书，阅读我可以赚钱，打开一个勤俭建国的商店我可以生活小康杂志我可以设计一个发动机64 英里一加仑汽油我可以做出新的抗生素我可以使电脑的生存，水生条件我知道如何执行业务我可以让你想购买的产品提出动议的热点和生产者我和我的朋友们了解未来我看到的字符串控制系统我可以做任何事情，没有援助 cuz我可以导致一个民族，一个麦克风与麦克风与麦克风我可以分裂了原子的分子一个分子一个分子看我看我驾驶，我不会停止和感觉那么好要活着就顶端我的达成是全球我的大楼安全我的事业是高尚的我的权力是纯我可以伸手万疫苗接种或let'em都死在无奈 have'em全部愈合他们的割伤 have'em所有杀害暗杀我可以作出任何人去监狱只是因为我不和like'em 我可以做任何事情，没有许可我一切都在我的指挥因为我可以引导导弹卫星通过卫星通过卫星我可以击中目标，通过望远镜透过望远镜透过望远镜我可以结束的星球在大屠杀在大屠杀在大屠杀在大屠杀在大屠杀在大屠杀我可以坐我的自行车没有把手没有把手没有把手我可以坐我的自行车没有把手没有把手

The sixteen plants are firstly divided into six types according to evaluation grades and subject function statistical method:the first type is Cajanus cajan and Crotalaria mucronata,the best adaptability ,which can be used pioneer plant or constructive species; the second type is Cynodon dactylon,the better adaptability, which can be used constructive species ;the third type is Eragrostis curvula, Paspalam notatum,which can be used constructive species on rotten rock slope ; the forth type is kummerowia striata,Lotus corniculatus and Vitex negundo.The adaptability is centered,and can be used protection species and auxiliary species;the fifth type is Robinia pseudoacacia,Eremochloa ophiuroides,Vitex negundo,Lespedeza formosa,Leucaena leucocephala,Desmodium sequax, Rhus chinne- nsis,which can't be plant singly and be used accessory species;the sixth is Trifolium repens,which can be used pioneer plant.

根据评价边坡防护植物适应性的十个重要指标，首次运用隶属函数和聚类分析方法，对各供试植物的边坡适应性综合排序并分为六类：第一类为木豆和猪屎豆，边坡适应性最强，适宜作为先锋植物和建群种；第二类为狗牙根，边坡适应性强，适宜与豆科植物混播，作为建群种；第三类为弯叶画眉草和百喜草，边坡适应性较强，适宜与豆科植物混播，作为风化岩边坡的建群种；第四类为鸡眼草、百脉根和马棘，边坡适应性中等，适宜作为保护种与伴生种；第五类为刺槐、假俭草、荆条、美丽胡枝子、银合欢、波叶山蚂蝗和盐肤木，边坡适应性较差，不宜单独在边坡上种植，最好与速生型豆科草本混播，作为边坡防护植物群落的辅助植物；第六类为白三叶，边坡适应性最差，但因其出苗率高、生长速度快，作为先锋植物，起到前期覆盖坡面的作用。

Feiyes.net ★ rodick 制作 Cause it's hard for me to lose In my life I've found only time will tell And I will figure out that we can baby We can do a one night stand, yeah And it's hard for me to lose in my life I've found outside your skin right near the fire That we can baby We can change and feel alright I'm a little used to wandering outside the rain You can leave me tomorrow if it suits you just the same But I don't know enough, I need someone who leaves the day Hey yeah Cause it's hard for me to lose In my life I've found only time will tell And I will figure out that we can baby We can do a one night stand, yeah And it's hard for me to lose in my life I've found outside your skin right near the fire That we can baby We can change and feel alright Free Loop－－Daniel Powter Cause it's hard for me to lose In my life I've found only time will tell I will figure out that we can baby We can do a one night stand, yeah And it's hard for me to lose in my life I've found outside your skin right near the fire That we can baby We can change and feel alright Cause it's hard for me to lose In my life I've found only time will tell And I will figure out that we can baby We can do a one night stand, yeah And it's hard for me to lose in my life I've found outside your skin right near the fire That we can baby Over...

Feiyes.net ★ rodick制作原因很难对我来说，失去在我的生命中我发现只有时间才能告诉我们我将找出我们能够婴儿我们可以做一个一夜情，是啊我们很难对我来说，失去在我的生命我发现你的皮肤外面附近的火灾我们能够婴儿我们可以改变和感觉好吗我有点用以外的雨游荡你可以离开我明天如果适合你一样但我不知道不够，我需要有人谁离开天嘿是啊原因很难对我来说，失去在我的生命中我发现只有时间才能告诉我们我将找出我们能够婴儿我们可以做一个一夜情，是啊我们很难对我来说，失去在我的生命我发现你的皮肤外面附近的火灾我们能够婴儿我们可以改变和感觉好吗免费环路-丹尼尔Powter 原因很难对我来说，失去在我的生命中我发现只有时间才能告诉我们我会弄清楚，我们才能婴儿我们可以做一个一夜情，是啊我们很难对我来说，失去在我的生命我发现你的皮肤外面附近的火灾我们能够婴儿我们可以改变和感觉好吗原因很难对我来说，失去在我的生命中我发现只有时间才能告诉我们我将找出我们能够婴儿我们可以做一个一夜情，是啊我们很难对我来说，失去在我的生命我发现你的皮肤外面附近的火灾我们能够婴儿超过。。。

Touch need your hands on me so we can touch i need your lips on me so we can touch boy lick my body weak come and gimme what i need fulfill my fantasies so we can touch ah ah ah touch ah ah ah ah ah ah touch ah ah ah i can't wait no more got this feelin' in my mind baby i'm gonna call you up and yes i know it's late at night but i can't hold back the urge wanna hear you in my ear wanna feel our bodys merge boy i need you lyin' here so we can touch put your hands on me so we can touch put your lips on me wanna feel your touch boy lick my body weak come and gimme what i need fulfill my fantasies so we can touch ah ah ah touch ah ah ah touch ah ah ah touch ah ah ah boy i hope you're ready this gon' be a long night we're gon' do it how you want it 'cause i know just what you like got my body feelin' right my emotions runnin' high and i'm feelin' kinda lovely sure there's something we should try we can touch put your hands on me so we can touch put your lips on me wanna feel your touch boy lick my body weak come and gimme what i need fulfill my fantasies so we can touch ah ah ah touch ah ah ah touch ah ah ah touch ah ah ah look ma i'm glad you called me i was just about to turn around anyway i've been thinkin' bout that move that you made up you mean the one i call the stand up huckle buck yeah ma i love it when you do you i know if you hurry up i ma give you part 2 i'm on my way girl can't you hear my car rushin' i'm tryin' to get there fast so we can sart touchin' touch put your hands on me so we can touch put your lips on me wanna feel your touch boy lick my body weak come and gimme what i need fulfill my fantasies so we can touch put your hands on me so we can touch put your lips on me wanna feel your touch boy lick my body weak come and gimme what i need fulfill my fantasies so we can touch ah ah ah touch ah ah ah touch ah ah ah touch ah ah ah touch

触摸需求你的人手右手击球员的左后方场地自我如此我们罐触摸i需求你的嘴唇右手击球员的左后方场地自我如此我们罐触摸男孩舔我的躯体弱来和乞讨的什么 i需求履行我的幻想曲如此我们罐触摸啊啊啊触摸啊啊啊啊啊啊触摸啊啊啊i不能等候不再得到这个feelin在朝派我的介意当作婴儿般对待我是去向打电话你向上的移动和同意i知道它's迟在晚上但是i不能支持住后退成为驱动力想要向听见你在朝派我的耳朵想要向感觉我们躯体合并男孩i需求你lyin'这里如此我们罐触摸放你的人手右手击球员的左后方场地自我如此我们罐触摸放你的嘴唇右手击球员的左后方场地自我想要向感觉你的触摸男孩舔我的躯体弱来和乞讨的什么 i需求履行我的幻想曲如此我们罐触摸啊啊啊触摸啊啊啊触摸啊啊啊触摸啊啊啊男孩i希望你公亩敏捷的这个gon'是a长时间晚上我们公亩gon'社交聚会它怎样你想要它'原因i知道仅仅什么你爱好得到我的躯体feelin权利我的情感runnin高度和我是 feelin可爱的人的一种可靠的那里's某样东西我们是将去请尝试我们罐触摸放你的人手右手击球员的左后方场地自我如此我们罐触摸放你的嘴唇右手击球员的左后方场地自我想要向感觉你的触摸男孩舔我的躯体弱来和乞讨的什么 i需求履行我的幻想曲如此我们罐触摸啊啊啊触摸啊啊啊触摸啊啊啊触摸啊啊啊看妈我是很高兴你打电话自我i was差不多向转变不管怎样我富人是讨论会'较量那个移动那个你制做向上的移动你用意一 i打电话竖立向上的移动髋部反对是的妈i爱它什么时候你社交聚会你i知道条件你匆忙向上的移动i妈让步你分开2我是右手击球员的左后方场地我的方式女孩不能你听见我的小汽车rushin'我是tryin'向到达那里斋戒如此我们罐sart touchin'触摸放你的人手右手击球员的左后方场地自我如此我们罐触摸放你的嘴唇右手击球员的左后方场地自我想要向感觉你的触摸男孩舔我的躯体软弱情欲高潮和乞讨的什么样的i需求履行我的我们能触动表达的幻想曲如此你的我们能稍微谈到一点自我如此的人手放你的我的幻想曲啊啊啊稍微谈到一点自我贫穷，感受到你的触摸男孩舔我的i需求履行什么软弱情欲高潮和乞讨的躯体，所以我们罐的嘴唇 touch ah ah ah touch ah ah ah touch ah ah ah touch

Achieve a special aesthetic culture of Japan in the world, Japanese men have used aesthetic culture to control and judge class or age and social position of Japanese women, KOGAI MAGE and TAKASHIMADA MAGE is used by common women, KATSUYAMA MAGE and TENJIN MAGE is used by geshia women, HYOKO MAGE is used by concubine, RAKAKU MAGE is used by servant, SANRIN MAGE is used by young women, KUMEZO MAGE is used by a middle-aged women, MARU MAGE is used by women of old age.

日本古代发型是最足以代表日本风情的民族发式，翻开日本古代发型的演变史；最早可推到3世纪的古坟时代，历经千年以上之改良创新，而发展出多彩多姿的款式。皇族之笄髷及上流社会之高岛田髷，女医及巫女等特殊职业之片手髷，普通国民流行之桃割髷及银杏髷，艺妓专用之胜山髷及天神髷，女妾专属之兵库髷，女佣之螺角髷；少妇的三轮髷；中年之久米三髷；老年之丸髷，均有各领风骚的发型区别，毛发款式描法依照人体脸型七种样式来美化。

Canadians can campaign for Québec libre. Americans can support separatists in Puerto Rico, Vermont, Texas, California, Hawaii, Guam, and Alaska. Brits can work for a free Wales, and Scotland for the Scots. French can help free Tahitians, New Caledonians, Corsicans, and the Basques. Spaniards can also back the Basques, or the Catalonians. Italians can help Sicilian separatists or the Northern League. Danes can free the Faeroe Islands. Poles can back Cashubians. Japanese can help Okinawan separatists, and Filipinos can help the Moros. Thai can promote Patanni independence; Indonesians can promote Acehnese independence. New Zealanders can leave the islands to the Maori; Australians can vacate Papua. Sri Lankans can help Tamil separatists; Indians can help Sikh separatists.

加拿大人可以参加魁北克独立运动；美国人可以支持波多黎各，佛蒙特州，德克萨斯州，夏威夷，关岛和阿拉斯加州的分离主义者；英国人可以为争取威尔士独立，苏格兰人可以争取苏格兰独立；法国人可以帮助塔西提人，新的古苏格兰人，科西嘉人和巴斯克人获得自由；西班牙人可以支持巴斯克人或者卡泰罗尼亚人；意大利人可以帮助西西里岛分离主义者或者北部联盟；丹麦人可以还法罗群岛自由；波兰人可以支持Cashubians；日本人可以帮助冲绳岛分裂主义者；菲律宾人可以帮助摩洛族，泰国人可以推动帕坦独立；印尼人可以推动亚齐独立；新西兰人可以把岛屿归还给毛利人；澳大利亚人可以撤离巴布亚；斯里兰卡可以帮助塔米尔分离主义者；印度可以帮助锡克教分离主义者。

A big wood can be used as a girder, a thin wood can be used as a square rafter, a small wood can be used as a pendentive, a stout wood can be used as a door-hinge pit, and a wedge can be used as a door bar.

大的木材能被同样地用一个桁，瘦的木材能被同样地用一个正直的椽，小的木材能被当作 pendentive 使用，强壮的木材能被当作一个门-铰链深坑使用，而且一片楔子能被当作一扇门使用除之外。

Salt is good, but if the salt becomes flat and tasteless, with what do you season it?

14：33 盐本是好的，盐若失了味，可用什么叫它再咸呢？

He reiterated that the PLA is an army of the people under the leadership of the Communist Party of China.

他重申，人民解放军是在中国共产党领导下的人民军队。