查询词典 callus

ABSTRACT It is because that the excellent acceptors for maize transgenic engineering are insufficient in our country, especially in the southwest mountain areas of china and hereditary variation regularity for the two characters such as efficiency of embryonic callus induction and number of regenerating plant (these two characters were abbreviated to the nduction efficiency and number of regenerating in the following of the paper, respectively), which hint the maize culturing capacity, is not very clear. Therefore, aiming at picking out superior acceptors, we had made systematic researches on the two characters with combing traditional quantitative-character genetic analyzing methods such as single-factor genetic mating design, diallel crossing genetic design, genetic effect analyzing method and the modern molecular locating method such as QTLs'. The main results are followed.(1) 50 superior inbred lines and about 30 crosses in our country, especially in the southwest of China were used for identifying and selecting the superior genotypes in the above two investigated characters under the same culturing condition in 2000 and 2001. There was very significant difference among the genotypes in the both characters. But the two characters were not certainly related. Some genotypes such as 18-599 and 18-599 were very good in them. For some ones such as zong 31, induction was higher than 18-599 and 18-599 in the efficiency, but it was only 1/3 to the later in regenerating number. In some genotypes such as S37, R08, R15, P138, A318, induction efficiency was just about 3% and scarcely any regenerating plants were got. On the whole, hybrids acted better than inbreeds in the both characters.(2) Two kind of inbreeds were selected as parents of the Griffing's method 1. 18-599 and 18-599 and the inbred line zong 31 are one kind because they are not only superior in the characters of maize cross breeding, such as CA, resistance to disease and the important agricultural characters, but also excellent in transformation characters as the induction and regeneration.

针对我国、特别是西南山地所需玉米转基因工程育种优良受体极为匮乏和反应玉米幼胚培养能力的2个主要性状，即玉米幼胚胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数的遗传变异规律十分不清楚的实际情况，本研究从筛选玉米转基因工程所需要的优良受体入手，采用单因素遗传交配设计、双列杂交遗传交配设计、世代基因效应等传统数量性状分析方法，以及现代分子标记定位主效QTL分析方法，对玉米幼胚胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数等2个性状进行了较为系统的分析研究，取得以下主要研究结果：(1)于2000年和2001年通过对我国、特别是西南地区近50份优良自交系和近30个杂交组合，在相同培养条件下，对幼胚培养胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数等2个幼胚培养能力性状进行了筛选与鉴定，发现玉米不同基因型具有完全不同的幼胚培养胚性愈伤组织诱导率和胚性愈伤组织绿苗发生数，但幼胚培养胚性愈伤组织诱导率与胚性愈伤组织绿苗发生数并不具有必然的相关关系，有的基因型，如自交系18-599和18-599在胚性愈伤组织诱导率和愈伤组织绿苗发生数等2个性状都表现相当优异；有的基因型，如自交系综31，仅幼胚培养胚性愈伤组织诱导率性状表现高于19-599和18-599，但在胚性愈伤组织绿苗发生数这一性状则与它们有相当大的差距，仅为19-599和18-599的1/3左右；有的基因型，如S37、R08、R15、P138、A318等玉米自交系不仅幼胚培养胚性愈伤组织诱导率很低，平均仅在3％左右，而且胚性愈伤组织绿苗发生数表现也很差，基本上没有分化成苗。

Spikelets with 1 floret (to 3 in Duthiea ), terete or laterally compressed, disarticulating above glumes; rachilla extension usually absent (present in Stephanachne , Sinochasea , and Duthiea ); glumes equaling or longer than floret, persistent, hyaline to membranous, 1–7-veined; floret callus obtuse to pungent; lemma rounded on back, membranous to leathery, firmer than glumes, often indurated at maturity, 3–9-veined, often enclosing palea, apex entire or 2-toothed, awned from apex or between teeth; awn straight or geniculate, glabrous, pubescent or plumose; palea subequal to lemma, hyaline to membranous, usually 2-veined (5–7-veined in Psammochloa ) but not keeled, apex usually acute.

小穗具1小花(对3在Duthiea里)，圆柱状的或侧面压扁，高于颖片脱节；小穗轴扩展通常无(在Stephanachne，Sinochasea 和Duthiea里提出)；颖片等长或更长于小花，宿存，到膜质，1-7脉；对刺激愚蠢的小花结茧；外稃在后面上绕行，膜质到革质，比颖片坚定，芒直或者膝曲，无毛，青春期或者羽状；内稃近等长于外稃，透明的到膜质，通常2脉的(在Psammochloa里的5-7脉)但不是翻转，先端通常厉害。

Therefore need careful nursing partial callus skin, avoid injury of the scalp hair follicle transplantation, the patient is unable to pick the scab Jie transplant skin, not in January-February after the "delinking eyebrow period" arbitrary use of drugs Sticking to avoid outsmarting themselves.

因此需精心护理局部的痂皮，以免损伤移植头皮的毛囊，患者既不能自行挑揭移植区的痂皮，更不应在术后一至二月的&脱眉期&任意使用药物敷贴，以免弄巧成拙。

Adding BA in MS+CH media raised callus formation and the percentage of the podding without root.

添加BA促进愈伤组织生成，但提高未发根结荚率。

Adding both NAA and BA to medium would promote the percentage of callus formation of tip and podding without root, but would inhibit the podding with root.

同时添加NAA及BA对子房柄形成愈伤组织及不发根结荚有加成的促进作用，但对发根结荚则有加成的抑制作用。

In addition, the repair method can also be filled tree holes, stuffing is best to use small gravel and cement mixture to be compacted filler, filler to strengthen the connection with the xylem, inside the cave can be a number of electroplating nails nails, holes dug in on both sides 4 centimeters deep groove at the bottom of filler from the start, each 20-25 cm for the layer, using linoleum separated from each floor should be inclined slightly outward to facilitate drainage, the edge should not exceed xylem, so that cambium in the formation of callus on it.

另外，也可用填充法修补树洞，填充物最好用小石砾和水泥的混合物，填充物必须压实，为加强填料与木质部的连接，洞内可钉若干电镀铁钉，洞口内两侧挖4厘米深的凹槽，填充物从底部开始，每20厘米至25厘米为一层，用油毡隔开，每层都要向外略斜，以利排水，边缘应不超过木质部，使形成层能在它上面形成愈伤组织。

Different callus were obtained by culturing the leave explants of one double flower Petunia plant on the media supplemented with different combinations of auxin and cytokinnins.

方法］以重瓣矮牵牛叶片为外植体，使用不同浓度BA与NAA的组合在黑暗条件下诱导愈伤组织。

Li Baiqing: In the hand is the callus, just sat the wheelchair, the equilibrium sense was not very good, the training instructs slowly, slowly overcame.

李柏青：手上都是老茧，刚坐上轮椅，平衡感都不是很好，教练慢慢指导，都慢慢克服了。

The result provide a basic foundation to produce transgenic alfalfas that are resistant against salt stress by introducing Na+/H+ antiporter gene from synechocystis species pcc 6803.Key words gene engineering Medicago sativa L. hypocotyls callus tissue culture

本试验结果为下一步将来自蓝藻的Na+/H+ an-tiporter基因转入苜蓿，获得转基因耐盐苜蓿奠定了基础。

So acertain concentration of auxins is important to the induction of embryonic callus, and cytokinins are cooperative in the process of somatic embryo genesis and embryoid growth.

因此，一定浓度的生长素是诱导紫叶酢浆草胚性愈伤组织的关键因素，细胞分裂素在体细胞胚的发生和发育过程中起增效作用。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。