查询词典 callus

Key words Poncirus trifoliatea ; callus tissue; CaCO3 stress; physiological and biochemical index

关键词枳壳；愈伤组织；碳酸钙(CaCO3)胁迫；生理生化指标

The results showed that the non-embryonic callus were cultured on the MS medium contained 4.0 mg · L-1 2,4-D and 0.5 mg · L-1 6-BA, embryogenic cells appeared in it. Then the single embryogenic cells formed a proembryo composed of many cells through cell division.

结果表明，非胚性愈伤组织转入到MS + 4.0 mg · L-1 2，4-D + 0.5 mg · L-1 6-BA培养基培养后，愈伤组织中先出现一些胚性细胞；然后单个胚性细胞分裂形成一个由多细胞组成的原胚。

The reseach on activity changes of SOD, POD and CAT during the somatic embryogenesis of Y35 showed:(1) The activty of SOD was from 52.98 to 133.20 U·g-1·h-1, and remained a rising trend after early single embryo forming, this revealed that SOD might be positively correlated to the differentiation of embryogenic cell and the development of somatic embryo.(2) The activty of POD was from 0.05 to 0.50 U·mg-1·min-1, ascended firstly and desceded later, and was highest in embryogenic callus and lowest in late single embryo , this revealed that POD might be positively correlated to the division and differentiation of proembryo mass, while negatively correlated to the development of PEMⅢto late single embryo.(3) The activty of CAT was from 0.86 to 2.81 U·mg-1·min-1, showed an up-down-up trend, reaching to the highest peak at the time of early embryo formating and decreasing to the lowest at the time of early cotyledonary embryo formating, this revealed that CAT might be positively correlated to the development of early single embryo, while negatively correlated to the formation of middle single embryo and early cotyledonary embryo.The changes in activty of SOD, POD and CAT indicated these three antioxidant enzymes coregulated the differentiation and development of embryogenic cells during Larix somatic embryogenesis.4. Differentially expressed cDNA libraries of the stages of proembryo mass and somatic embryo maturation were successfully constructed by suppression subtractive hybridization.

对Y35体细胞胚胎发生过程中抗氧化酶活性变化的研究显示：(1)SOD活性在52.98~133.20 U·g-1·h-1之间，并在早期单胚形成后一直保持上升的趋势，表明其与胚性细胞的分化及体细胞胚的发育均具呈正相关；(2)POD活性在0.05~0.50 U·mg-1·min-1之间，呈现出先下降后升高的趋势，在胚性愈伤组织中最高，而在后期单胚形成时降至最低，表明其与原胚团的分裂和分化呈正相关，但与PEMⅢ向后期单胚的发育呈负相关；(3)CAT活性在0.86~2.81 U·mg-1·min-1之间，表现出升-降-升的变化趋势，在早期单胚形成时升至最高，在早期子叶胚形成时降至最低，表明其与早期单胚的发育呈正相关，而与中期单胚和早期子叶胚的发育呈负相关。

One case of congenital pseudoarthrosis of the tibia was operated twice and there were callus grown half years after the second operation, but reoccured after one year.

一次手术成功9例，经二次手术成功7例，1例先天性胫骨假关节经二次手术，术后6个月有新生骨生长，1年后再复发。

With tender stem of Hemistepta lyrata Bunge as material, the conditions needed in callus induction and differentiation, adventitious bud differentiation and radication, test tube seedling cutting and transplantation were studied.

方法］以泥胡菜嫩茎为材料，研究愈伤组织诱导和分化、不定芽的分化和生根、试管苗移栽和扦插及移植于山坡栽培所需要的条件。

The results of GUS histochemical assays and PCR amplification demonstrated that the overall transformation rate of Roselle callus was 4%.

GUS活性组织化学检测和PCR扩增鉴定的结果表明，愈伤组织的转化率为4%。

It is feasible to use Agrobacterium-mediated method to introduce exogenous gene into Roselle cells via callus.

说明采用农杆菌介导法将外源基因经愈伤组织导入玫瑰茄细胞是可行的。

Tumefaciens-mediated genetic transformation system of Roselle callus, AtPSK2 gene was introduced into and expressed functionally in Roselle cells.

利用玫瑰茄愈伤组织遗传转化体系，通过根癌农杆菌将AtPSK2基因导入玫瑰茄细胞，并获得了功能性表达。

The effect of different medium compositions on callus induction, proliferation, plantlet regeneration and plantlet rooting of Salicornia bigelovii Torr.

本试验研究了培养基成分对海蓬子愈伤组织诱导、继代培养、植株分化和生根的影响。

CALLUS INDUCTION AND PLANTLET REGENERATION OF Salicornia bigelovii TORR.

余桂红，柏文娟，马鸿翔，张旭，易金鑫

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。