查询词典 callus

Objective:To study the effect of several factors on the quantity of hypericin in H. perforatum callus.

中文摘要：目的：为了探讨几种理化因子对贯叶金丝桃愈伤组织中金丝桃素含量的影响。

In this study 2mG2-epsps gene was used to be a selection marker gene, and a series of experiments were designed for determining the time of their screening and selection pressure. The results showed that 1 mM glyphosate isopropylamine and 15 days screening can be effectively inhibited non-transgenic maize callus tissue differentiation. So this research work has provided a safe and effective screening system for corn and other monocotyledons crops.

实验中以2mG2-epsps基因作为筛选标记基因，为确定其筛选时间及筛选压力，进行了玉米愈伤组织对不同浓度草甘膦的抗性实验，确定向培养基中添加浓度为1mM的草甘膦异丙胺盐筛选15天能有效的抑制非转基因玉米愈伤组织的分化，本研究的再生植株的阳性率为45%，为玉米及其它单子叶作物转化提供了一种有效、安全的筛选体系。

When setting catharanthus roseus laminae, petiole, hypocotyl as explants, the effects of different kinds of homogeneity and concentrations of growth adjustive matter on inducing callus were different.

以长春花幼嫩叶片、叶柄、下胚轴为外植体，不同种类、不同浓度的植物生长调节物质及其组合对愈伤组织诱导的效应均不相同。

To study the molecular mechanism of Larix somatic embryogenesis, a differentially expressed cDNA library of Larix somatic embryo in the period of maturation was constructed using suppression subtractive hybridization. The cDNA from the cultures at the stage of somatic embryo maturation of embryogenic cell line Y35 of L. leptolepis ×L. principis-rupprechtii was used as the tester and the cDNA from its subcultured callus was used as the driver. Eight hundreds randomly selected positive clones were sequenced, and 468 UniGenes were obtained finally.

为研究落叶松体细胞胚胎发生的分子机理，文章以日本落叶松×华北落叶松杂种无性系胚性细胞系Y35体细胞胚成熟阶段培养物的cDNA为实验组，继代培养阶段胚性愈伤组织的cDNA为对照组，利用抑制性消减杂交技术(Suppression subtractive hybridization， SSH)构建了体细胞胚成熟阶段的差异表达基因文库。

To study the molecular mechanism of Larix somatic embryogenesis, a differentially expressed cDNA library of Larix somatic embryo in the period of maturation was constructed using suppression subtractive hybridization. The cDNA from the cultures at the stage of somatic embryo maturation of embryogenic cell line Y35 of L. leptolepis L. princi-pis-rupprechtii was used as the tester and the cDNA from its subcultured callus was used as the driver. Eight hundreds ran-domly selected positive clones were sequenced, and 468 UniGenes were obtained finally.

为研究落叶松体细胞胚胎发生的分子机理，文章以日本落叶松华北落叶松杂种无性系胚性细胞系Y35体细胞胚成熟阶段培养物的cDNA为实验组，继代培养阶段胚性愈伤组织的cDNA为对照组，利用抑制性消减杂交技术(Suppression subtractive hybridization， SSH)构建了体细胞胚成熟阶段的差异表达基因文库。

To study the molecular mechanism of Larix somatic embryogenesis, a differentially expressed cDNA library of Larix somatic embryo in the period of maturation was constructed using suppression subtractive hybridization. The cDNA from the cultures at the stage of somatic embryo maturation of embryogenic cell line Y35 of L. leptolepis × L . princi-pis-rupprechtii was used as the tester and the cDNA from its subcultured callus was used as the driver. Eight hundreds ran-domly selected positive clones were sequenced, and 468 UniGenes were obtained finally.

为研究落叶松体细胞胚胎发生的分子机理，文章以日本落叶松×华北落叶松杂种无性系胚性细胞系Y35体细胞胚成熟阶段培养物的cDNA为实验组，继代培养阶段胚性愈伤组织的cDNA为对照组，利用抑制性消减杂交技术(Suppression subtractive hybridization， SSH)构建了体细胞胚成熟阶段的差异表达基因文库。

Cotyledons and hypocotyls of Lesquerella fendleri were used as explants to induce callus on MS media with different hormone combinations.

以Lesquerella fendleri下胚轴和子叶作为外植体，研究了不同的激素浓度和组合对於愈伤组织诱导和植株再生的影响。

The method was rapid, simple, accurate and stability, and can be used for the quantitiative determination of Ferulic acid in Ligusticum chuanxiong callus.

该方法快速简便、准确可靠，可用于川芎愈伤组织中阿魏酸的含量测定。

The research results will conduce to the exploration of Mahonia plants.3^ In the experiment, we induced callus with the M.eurybracteata.

用HPLC法对收集到的材料茎中三种主要生物碱(小檗碱、药根碱、巴马汀)的含量进行了分析。

To induce callus tissues and establish suspension culture of Marchantia polymorpha L.

目的探计对地钱愈伤组织的诱导和悬浮细胞培养的条件。

And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时，论述了一体化管理体系构建中的几个主要问题，包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。