查询词典 calli

About calli from different explants, there were significant differences in their resveratol content, and their medium of the highest content expressed were respectively: for leaves, B5 medium+6-BA 0.5 mg/L+IBA 0.2 mg/L; for leafstalk, B5 medium+6-BA 1.0 mg/L+IBA 0.2 mg/L; for stems, B5 medium+6-BA 0.5 mg/L+IBA 0.2 mg/L.

以叶、叶柄和茎为外植体诱导愈伤组织时，其白藜芦醇含量最高的培养基分别为B5培养基+6-BA0.5mg/L+IBA0.2mg/L、B5培养基+6-BA1.0mg/L+IBA0.2mg/L和B5培养基+6-BA0.5mg/L+IBA0.2mg/L。

It was demonstrated that the BADHgene promoter region and its two mutants are active in monocot rice calli.

实验表明，质粒pDMC202中GUS基因在BADH基因启动区以及两个启动区突变体的控制下获得了表达。

Here, we inserted the RRD3 fragment into the plant promoter-capture vector, pCAMBIA1391Z, and examined whether the RRD3 fragment has promoter activity in plants. Transgenic tobacco and rice calli both showed β-glucuronidase activity, indicating that RRD3 can act as a promoter in both monocot and dicot plants.

我们将RRD3片段插入到植物启动子捕获载体pCAMBIA1391Z中，检测RRD3片段是否在植物中也有启动子活性，转基因烟草再生植株和水稻愈伤组织均显示了gusA基因的表达，表明其在单子叶和双子叶植物中均可行使启动子功能。

In order to evaluate the effect of over-expressed Mn-superoxide dismutase gene on antioxydation in maize, expression vector with Mnsod gene from wheat was constructed for monocotyledonous plant and used to transform calli of elite maize inbred line by microprojectile bombardment.

为研究过量表达的超氧化物歧化酶基因对提高玉米抗氧化损伤的作用，本研究构建了小麦来源Mnsod基因的单子叶植物表达载体，用基因枪法转化用优良玉米自交系诱导的胚性愈伤组织。

Culture tender leaves in culture medium of MS+2,4-D1.5mg/L+30g/L suger+0.7% agar pH5.8 for 20 days in darkness at 25℃, and then subculture to induced Ⅱ-type calli. Use forceps cutting the tissue to nubble with 2mm2, and put the tissue into Agrodacterium tumefaciens LBA4404 liquid supplemented with AS 100mg/L,then, co-culture 3 days, resume 7 days in MS+2,4-D1.5mg/L+30g/L suger+500mg/L cef, take to MS+2,4-D1.5mg/L+30g/L suger+100mg/L cef+10.0mg/L kanamycin culture 20 days in darkness. After that to make it polarize in MS+30g/L suger+100mg/L cef+10.0mg/L km. The percentage of km resistant callus was reached max after infection for 45 min, the average is 29.66%. Simultaneity, tender leave callus are infected 5 min by A. tumefaciens liquid in different negative pressure, and kept on 15 min in Agrodacterium tumefaciens liquid without negative pressure. Then screen out resistant callus and obtain transgenic plants. When the negative pressure is -0.05MP the percentage of km resistant callus was reached max, the average rate is 37.5%.

将心叶接种于MS+2.4-D1.5mg/L+30g/L 蔗糖，琼脂0.7%，pH5.8 培养基中25℃暗培养20d 后继代一次，诱导产生Ⅱ型胚性愈伤组织，用镊子将其夹碎成2mm2大小的小块，置入添加100mg/L AS 的根癌农杆菌LBA4404 菌液中，侵染时间为45min，共培养3 天后，转入MS+2.4-D1.5mg/L+30g/L 蔗糖+500mg/L Cef 培养基中恢复7天，再转入MS+2.4-D1.5mg/L+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 中，遮光培养20d后，置入其MS+30g/L 蔗糖+100mg/L Cef+10.0mg/L Km 分化，卡那霉素抗性愈伤组织获得率在侵染45min 时达到最大值平均为29.66%；同时以甘蔗心叶愈伤组织材料，通过循环水式真空泵，产生负压，设定不同的负压值，在农杆菌菌液中感染5min 后，在负压条件下继续侵染15min 后，筛选出抗性愈伤组织并获得转化植株，其中在负压为-0.05 时转化率达到最大值，其卡那霉素抗性愈伤组织获得率平均为37.5%。

Under the solid culture conditions,the effects of source of explants,medium,phytohormone,inoculating quantity and culture days on growth of calli and content of flavonoid in Cyclocarya paliurus were studied.

在固体培养条件下，研究了不同外植体来源、培养基、植物激素、接种量、培养时间对青钱柳愈伤组织生长和黄酮含量的影响。

The pokeweed calli can develop into five different colours,pink,red,white,light-yellow-green,brown.

在愈伤组织继代过程中产生的粉红色、红色、白色、黄绿色和褐色五种不同的愈伤组织细胞品系中，粉红色品系生长快，扩增率高。

Calli of Anthurium andraeanum 'Jolanba'derived from leaves of in vitro plantlets were cultured with three different treatments to observe their redifferentiation behaviors , and their physiological and biochemical features are discussed. The results went as followed.

对花烛 'Jolanba'无菌苗叶片诱导产生的愈伤组织进行3 种不同的培养处理，观察其再分化表现，并探讨了3 种继代培养愈伤组织的生理生化特点。

The traits of dedifferentiation and redifferentiation of the explants form H. sampsonii show that the calli the stem and leaf usually form buds at its parietal cells. In the presence of auxin, the parenchyma of the leaf gap at the node is initiated firstly, then, develops blastemata that form buds in a cluster on the node without callus. These show that the various parts ofthe herb differ greatly in cell initiation and organ differentiation.

研究元宝草外植体的脱分化和再分化的特点结果表明，茎段和叶片启动形成愈伤组织，其不定芽通常发生在愈伤组织的周边区，茎节叶腋的叶隙薄壁组织在激素的诱导下启动、分裂，不形成愈伤组织而直接器官分化成芽原基，并在节结处产生一圈成簇的不定芽，表明元宝草不同部位的细胞启动及器官分化调控存在差异。

Results indicated that, among the three kinds of culture mediums, NB medium worked best in the calli induction and gene transformation and was most suitable for tissue culture of Nipponbare scutellum.

结果表明，在三种培养基中，NB培养基对愈伤组织的诱导效果最好，转化效率最高，最适合于日本晴成熟胚的组织培养。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。