查询词典 calli

The leaves, leafstalk, and stems from grapes were used as explants to induce calli and subculture in medium (100 mL B5 medium containing 3 g sucrose and 0.6 g agar) with different plant hormone combinations (6-BA, KT, IBA, 2, 4-D), and then the resveratol content from those calli was determinated by using HPLC.

以葡萄的叶、叶柄和茎分别作为外植体，在不同激素组合（6-卞氨基嘌呤(6-BA)、激动素、吲哚丁酸、2，4-二氯苯氧乙酸(2，4-D)）的培养基（以B5培养基为基本培养基，含30g/L蔗糖和6g/L琼脂）上，进行愈伤组织的诱导和继代培养，并利用高效液相色谱法对不同来源愈伤组织中的白藜芦醇含量进行测定。

Inducible and constitutive expression vectors for monocotyledonous plants were constructed and used to transfer into maize calli. Plants were differentiated and regenerated from the positive calli screened with gradient concentration of hygromycin. 4 transformed plants with inducible expression vector and 2 with con

PCR检测获得4株用诱导型表达载体转化的植株和2株用组成型表达载体转化的植株，为进一步研究抗逆相关转录因子基因分eb在单子叶中的表达调控，培育抗逆玉米材料打下基础。

The results showed as follows: 3mg/LBA in differentiation medium was favorable for regeneration of callus; addition of 3mg/LABA, sucrose substituted with maltose, or sucrose partially substituted with mannitol in subculture media improved the status of callus in a degree and enhanced the differentiation frequency; partial desiccation treatment significantly increased the regeneration frequency of callus; addition of AgNO3 or CuSO4 in differentiation medium contributed to plant regeneration , The two resistant calli and fifteen regenerated plants were obtained when the calli of mature embryo of 7001S were infected with Agrobacterium carrying bar and CPTI gene.

结果表明：3mg/LBA对分化有利；继代培养基中加入3mg/LABA，以麦芽糖代替蔗糖，或以甘露醇代替部分蔗糖在一定程度上可以改善愈伤组织状态，提高分化率；干燥处理能明显提高愈伤组织分化率；分化培养中添加一定浓度的AgNO3和CuSO4有利于愈伤组织分化。借助农杆菌介导bar基因和CPTI基因对水稻进行遗传转化，获得2块抗性愈伤组织和15棵再生苗。

The results showed as follows 3mgLBA in differentiation medium was favorable for regeneration of callus; addition of 3mgLABA, sucrose substituted with maltose, or sucrose partially substituted with mannitol in subculture media improved the status of callus in a degree and enhanced the differentiation frequency; partial desiccation treatment significantly increased the regeneration frequency of callus; addition of AgNO3 or CuSO4 in differentiation medium contributed to plant regeneration , The two resistant calli and fifteen regenerated plants were obtained when the calli of mature embryo of 7001S were infected with Agrobacterium carrying bar and CPTI gene.

结果表明：3mgLBA对分化有利；继代培养基中加入3mgLABA，以麦芽糖代替蔗糖，或以甘露醇代替部分蔗糖在一定程度上可以改善愈伤组织状态，提高分化率；干燥处理能明显提高愈伤组织分化率；分化培养中添加一定浓度的AgNO3和CuSO4有利于愈伤组织分化。借助农杆菌介导bar基因和CPTI基因对水稻进行遗传转化，获得2块抗性愈伤组织和15棵再生苗。

The tolerant capacity of stem calli towards the ethylene inhibitor cobalt ion is lower than that of radicle calli.

朱砂根的茎愈伤组织对乙烯抑制剂Co〓的耐受性比根愈伤组织差。

Subsequently, on the basis of the system, the vectors pBar-1C and pBar-1Ab were transformed. 156 and 115 of resistant calli were obtained from 5400 and 4800 calli, respectively. Their transformation efficiency were 2.8% and 2.4%, and differentiation rates were 80.7% and 87.8%, respectively. These results suggest the transformation system is stable as well as efficiency, and good for transformation of rice with Bar gene as selective marker.

随后，以此体系为基础，将装有不同基因的两个载体pBar-1C和pBar-1Ab进行了转化，分别从5400块和4800块愈伤组织中获得156个和115个抗性愈伤，其抗性愈伤率分别为2.8%和2.4%，分化率分别为80.7%和87.8%，由此说明，此转化体系相对稳定，且分化率高、抗性愈伤率也较高，可用于同类选择标记的遗传转化，加快水稻转基因育种步伐。

Segments produced clusters of somatic embryos from embryogenic calli or directly from root apical meristems , and that also can convert into bud directly in the different tissue culture conditions. Modified media B5 with 1.0 mg.L-1 2,4 - D and 3.0mg.L~1 6-BA is suit to induce calli, and 0.5 mg.L-1 NAA is fit for somatic embryos inducement from calli. 6-BA encreased direct somatic embryogenesis on root tip distinctly,but NAA is retard it. NAA also prolong the time of root-tip convert into bud directly.

在改良B_5(微量元素为原来的1／3)中加入1.0mg.L~(-1)2，4—D和3.0mg.L~(-1)6-BA对离体根尖的愈伤组织诱导较为合适，0.5mg.L~(-1)NAA有利于从愈伤组织上产生体细胞胚；6-BA明显促进离体根尖直接产生类原球茎，而NAA则有阻碍作用；同时NAA的存在也使离体根尖直接成芽的时间延长。

The development of the calli and single few-celled aggregates was observed through paraffin sections.The results revealed 5 pathways of embryogenic calli origination including origination from both ends of hypocotyls,origination in inner,differentiation from non-embryonic cells,origination from outer cells of calli,and origination in the surrounding of gland.

以陆地棉品种Coker 201下胚轴为外植体诱导愈伤组织，并以介于30-50目筛之间的胚性愈伤细胞团为材料进行单个细胞团培养，采用石蜡切片法观察棉花愈伤组织诱导和分化过程及体细胞胚发生和发育过程。

The addition of malt extract and glutamine could inhibit non-embryonic calli, and make the culture be mainly composed of embryonic calli; change of the 2,4-D concentration in liquid culture medium could adjust the status of embryonic calli to the suitable developmental stage for protoplast isolation.

通过麦芽提取物和谷氨酰胺两种物质的添加，可以起到抑制非胚性愈伤生长的作用，使培养物主要以胚性愈伤为主；通过液体培养基2，4-D浓度的改变，可调节胚性愈伤处于合适的发育时期，便于原生质体的分离。

In the experiment, three thawing methods, such as washing by water at 40℃, exposing at the room temperature of 25℃, and washing by tap water were used to estimate the effects of thawing methods on the survival rate after cryopreservation. The results showed that the relative survival rate of the calli with proembryos was higher than that of the general calli; and that different thawing methods had the same effect on the survival rate of longan calli by cryopreservation.

结果表明，具有原胚分化的胚性愈伤组织保存后的存活率明显高于普通胚性愈伤组织；40℃温水浴、25℃左右室温和自来水冲洗等3种不同的化冻方式对龙眼胚性愈伤组织玻璃化超低温保存后的存活率具有同样的效果。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。