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However, they are anastomosing and not collapsed, and are accompanied by atypical endothelial cells, eosinophilic hyaline globules, plasma cells and fascicles of spindle cells.

然而,它们的血管网是吻合的,不塌陷,伴不典型内皮细胞、嗜碱性玻璃样小球、浆细胞和梭形细胞束。

To prepare the phosphorylated PRAS40 (Ser183) antibody, We chosen 10-amino acid including Ser183 as antigen peptide through antigenicity and hydrophobicity analysis, hinged on keyhole limpet hemocyanin, and used the KLH-peptide to immunize rabbits. After antibody serum titer detection by enzyme linked immunosorbent assay, the antibody was purified with rProtein A sepharose fast flow and dephosphorylated antigen membrane. The antibody titrate reached 1:10 000 after purification and its special property was enhanced with absorption treatment of dephosphorylated antigen membrane. In addition, we used rabbit anti-PRAS40 antibody and the phosphorylated PRAS40 (Ser183) antibody to detect PRAS40 expression in several cell lines, including the normal cells HL7702, HEK293, tumor cells HepG2, A549 and S180. There were no quite difference among these cells; otherwise, we observed the decreased phosphorylation level of Ser183 after amino acid withdrawal treatment.

为了制备PRAS40(Ser183)磷酸化多克隆抗体,本实验通过蛋白疏水性抗原性分析设计多肽抗原,用其免疫家兔获得抗血清, ELISA检测其效价为1:10 000; Western blotting法检测发现,通过rProtein A Sepharose亲和层析纯化并经非磷酸化的抗原条吸附处理后的抗体可以明显提高磷酸化抗体的特异性;用PRAS40抗体及PRAS40(Ser183)磷酸化抗体对正常细胞HL7702、HEK293及肿瘤细胞HepG2、A549、S180的检测显示:磷酸化的Ser183在不同细胞中表达差异不显著,而在经细胞饥饿处理的HEK293细胞中却明显观察到了S183磷酸化水平随氨基酸含量降低而减弱的现象。

Because the growth of tumor cells present parallel to theEGFR expression,so the significant increase of EGFR expression inHCC cells that laked serum may imply the comes of "explosivegrowth" when the HCC cells restore the blood supply.

因为肿瘤的生长与其EGFR的表达呈平行关系,所以因缺乏血液而引起的肿瘤细胞EGFR表达增高,可能意味着等得到血液供应后下一次&爆炸生长&的到来。

Insulin cells (insulin immunoeractive cells, B-cells) in the splenic lobi of the pancreas were labelled from 0 to 365 days old shao ducks with ABC immunohistochemical method.

用ABC免疫组织化学方法,标记0~365日龄绍鸭胰腺脾叶内胰岛素免疫反应细胞,用显微测微尺测量B细胞胰岛的面积,作统计学分析。

As CCR7 also mediates the homing of T cells to secondary lym- phoid organs (Willimann et al., 1998), it is a key receptor for the encounter of antigen-bearing mature dendritic cells and responder T cells.

作为CCR7 还介导的T细胞的归巢中学淋巴细胞淋巴器官( Willimann等。, 1998年),这是一个关键受体在遇到抗原轴承成熟树突状细胞和应答的T细胞。

Department of Haemotology,Zhongda Hospital,Southeast University,Nanjing210009,China)Abstract:Cytokine induced killeris a new kind of immune cells which have non MHC restric-tion,highly proliferation and cytotoxicity.Their activities are superior to lymphokine activated killer cells.Immunotherapy of CIK cells can significantly improve clinical symptoms,but hardly have any side effects.

摘要] 细胞因子诱导的杀伤细胞(cytokine induced killer,CIK)是一种非MHC限制性的新型免疫活性细胞,其增殖能力强、杀瘤活性高、副作用小,优于淋巴因子激活的杀伤细胞,对于提高患者自身抗肿瘤免疫能力有重要作用。

BACKGROUND: Basic fibroblast growth factor can accelerate the bone marrow mesenchymal stem cells proliferation and differentiation into nerve cells, which is considered as a mitogen of glial cells.

背景:碱性成纤维细胞生长因子可以促进骨髓间充质干细胞的增殖和向神经细胞方向分化,并被认为是胶质细胞的分裂原。

After selection of 7 days,some HB214 cells with F' factor from GM133 cells and lac+ mutation were observed,but these cells failed to form the colonies which can be seen by the naked-eye.

在培养了7d的选择平板上添加含链霉素的M9选择培养基,2d 后也观察到大量发生lac+突变但没有形成肉眼可见菌落的营养收集细胞。

For the first time, we found that the mosquito was a transmitted infectious media of malignant cells to human and quadrumana. The mosquito's body was full of malignant cells, and transmitted pathway was that the mosquito first spit out malignant cells from its thrusting tube and into human body, then it sucked the blood from human body into its stomach.

本文首次公开垦表,钻研发明蚊子是人类和灵长类动物恶性肿瘤传染媒介,蚊子刺针管内充满恶性肿瘤细胞,蚊子在吸血时入针后须先把针管内的肿瘤细胞吐出,然后才顺利把血液吸入胃内。

BACKGROUND: Research on seed cells is the most important aspect in the filed of tissue-engineering research, and because of their various ad vantages, bone marrow mesenchymal stem cells have been taken as the ideal bone tissue-engineering seed cells.

背景:种子细胞的研究是组织工程众多研究领域中最重要的基础环节,骨髓基质干细胞以其诸多优点,成为理想的骨组织工程的种子细胞。

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You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足,提出构建XML键的矢量矩阵作为窗口,利用矢量积小波变换多级分解与重构XML数据流,再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法,满足XML数据流质量重构时实时检测的要求,保证XML数据的约束性、一致性与完整性;为了更好的适应网络负载,采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。

This is a very big challenge to developers especially that Ajax is constantly changing.

这对开发者来说是一个非常大的挑战,尤其是需要不断变化的Ajax。