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Pulmonary microvascular endothelial cells, one of pivotal effective cells against pathopoiesis factor in early stage of disease, are proved to play an important role in mediating inflammatory response, aggravating tissue hypoxia, and secreting cytokines and matrix to regulate the migration, proliferation and differentiation of mesenchymal cells.

然而,它在消除致病因子对机体损伤的同时也可能由于某种原因而过度地发挥了介导免疫、炎症反应,加重组织缺氧,分泌过量细胞因子等生物学作用,从而促进了组织的纤维性修复。

The results showed that leaf epidermal cells were polygonous or irregular in shape. Stomatal complexes only present on abaxial epidermis and the stomata was paracytic type. There were five types of anticlinal walls of abaxial and adaxial cells (straight, rounded, undulated, sinuous, straight-curved). Trichomes on abaxial epidermis exist or not. Ornamention types of periclinal walls could be divided into punctuate, smooth and striate. Ornamention of periclinal walls on inner side of abaxial epidermal cells varied widely (irregular, smooth, roughly reticular, micro hairy, granular, scaly).

结果表明:润楠属植物叶表皮细胞形状为多边形或不规则形,气孔复合体均为平列型且仅分布在下表皮;上下表皮细胞的垂周壁式样有5种类型:平直、圆滑、技波、波状、平直-弓形;下表皮毛被有或无;上表皮平周壁纹饰类型有:点状、平滑、条纹;下表皮内面平周壁纹饰类型有:不规则、平滑、粗糙网状、微毛状、颗粒状、鳞片状。

The results showed that leaf epidermal cells were polygonous or irregular in shape. Stomatal complexes only present on abaxial epidermis and the stomata was paracytic type. There were five types of anticlinal walls of abaxial and adaxial cells (straight, rounded, undulated, sinuous, straight-curved). Trichomes on abaxial epidermis exist or not. Ornamention types of periclinal walls could be divided into punctuate, smooth and striate. Ornamention of periclinal walls on inner side of abaxial epidermal cells varied widely (irregular, smooth, roughly reticular, micro hairy, granular, scaly).

结果表明:润楠属植物叶表皮细胞形状为多边形或不规则形,气孔复合体均为平列型且仅分布在下表皮;上下表皮细胞的垂周壁式样有5种类型:平直、圆滑、浅波、波状、平直-弓形;下表皮毛被有或无;上表皮平周壁纹饰类型有:点状、平滑、条纹;下表皮内面平周壁纹饰类型有:不规则、平滑、粗糙网状、微毛状、颗粒状、鳞片状。

At 6 wk, the hepatic stem cells begin to differentiate to hepatocyte system, and then to cholangiocyte system in the next week. Like oval cells in the adult liver, the hepatic stem cells mainly locate at the periportal region at 10-12 wk.

wk, 肝干细胞开始向肝细胞系分化, 7 wk向胆管系分化, 10-12 wk,肝干细胞主要局限于汇管区周围的肝索,与成年肝中卵圆细胞的分布一致。

RESULTS: On day 2 after PH, oval cells began to proliferate around the portal area, and matrilin-2 deposition was observed in the hepatic sinusoids in the periportal area. On day 9, proliferating oval cells were present in the hepatic acini, and matrilin-2 upregulation was noted. On day 12, as oval cells differentiated to form hepatocellular nodules, matrilin-2 was distributed mainly in the periphery of the nodules, and little protein was present in the nodules.

结果: 肝脏部分切除术(partial hepatectomy, PH)后第2天,卵圆细胞开始向门静脉周围区域增殖, Matrilin-2主要出现在门静脉周围的肝窦状隙内;术后第9天,卵圆细胞进一步向肝实质内增殖, Matrilin-2表达增加;术后第12天,随着卵圆细胞分化为小肝细胞结节,大多数Matrilin-2位于结节周边,少数出现在结节内。

Foreign gene could destroy larvas mid-intestine, and reduce its appetite. Observation by microscope showed that the gaps between mid-intestine cells was extended in treated larvae, and the nucleus were destroyed, the gap between peritrophic membrane and mid-intestine cells was appeared, parts of peritrophic membrane was broken off from mid-intestine cells.

外源基因对舞毒蛾幼虫的杀虫机理在于破坏幼虫的中肠组织,影响幼虫的取食,使幼虫取食量减少,甚至终止取食;中肠细胞间隙增大,细胞核遭受破坏;围食膜与中肠细胞之间出现空隙,甚至脱落,围食膜不再完整;中肠组织脱落,成为一个严重变形的残架。

We generated EG4-GFP cell lines expressing enhancing green fluorescence protein which still maintained typical characteristics of pluripotent stem cells. We microinjected green cells into perivitelline of 8-cell stage embryos as well as aggregated them to 8-cell stage embryos with zona pellucida removed, to investigate developmental fate of EG4-GFP cells during formation of chimeric blastocysts.

我们建立了稳定表达绿色荧光蛋白的EG细胞系(EG4-GFP),发现该细胞仍能保持野生型的一般生长特性;通过显微注射和体外聚集得到细胞与8-细胞受体胚胎共同发育形成的嵌合胚,发现转基因细胞参与了胚胎早期发育过程中的生长与分化,大部分细胞集中于内细胞团,也有细胞分化为滋胚层细胞。

The giant panda-rabbit interspecies reconstructed embryos were constructed by injection of giant panda somatic cells into the perivitelline space of the enucleated oocytes and fused by electrical stimulation, and the reconstructed embryos can develop to hatched blastocyst stage in vitro. We demonstrated that the nuclear genetic materials in reconstructed blastocyst cells were the same as that of giant panda somatic cells by chromosome count and the existence of characteristic chromosome, which was confirmed by microsatellite DNA analysis later In order to determine the fate of mtDNA of giant panda-rabbit reconstructed embryos, we analyzed the cyt b sequence of embryos at 1 -cell stage, 2-cell stage, 4-cell stage, 8 cell stage, morula stage, blastocyst stage, expanded blastocyst stage and hatched blastocyst stage.

异种克隆大熊猫重构胚是通过将大熊猫成纤维细胞移入日本大耳白兔去核卵母细胞透明带下后经电融合构建的,可在体外发育至孵化囊胚阶段,我们应用一种简便、快速的异种重构胚的鉴定方法,即通过染色体数目和特征染色体的存在初步鉴定出大熊猫-兔异种重构胚的核遗传物质来源于大熊猫供核体细胞(后经昆明动物研究所通过微卫星DNA分析验证了该结果的可靠性)。

At early stage of cancer cell formation, provoked autophagy help to eliminate damaged cell organelle、and degrade the noxious substance in endochylema(eg: peroxidate)as well, pretect normal cells from cancerization.Autophagy level modulation might be cooperation or rivalry with chemotherapy to cancer cells compares to single use of chemotherapy drugs, apoptosis rate of cancer cells fluctuates with autophagy level.

癌细胞形成早期刺激自噬能够清除受损细胞器,分解细胞内过多的有害物质,阻止正常细胞向癌细胞转化,调控自噬的表达和化疗药物联合对肿瘤细胞的生长有协同或拮抗作用,表现为与单纯用化疗药物相比,调控自噬表达后肿瘤细胞的凋亡率发生了变化。

Cultured for 48 hours, the floating cells were removed and the adherent cells were continued to culture with cytokines including IL-4 and GM-CSF. After 14 days culture, the induced DCs were observed by photics microscope and electron microscope, also co-cultured with native T cells derived from spleen, its stimulating function was detected by MTT assay.

经过光镜,透射电镜观察培养DCs的形态学特征;通过同种T细胞混合培养,采用MTT比色分析法测定不同浓度的DCs发同种T细胞增殖的能力。

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