查询词典 caliciform cells

The brain tissue of the different fetal age rat could be cultured into neural stem cells with the most number of cells for 12.5 dags embryo, which furthermore differentiated into neurons and glial cells.

胎龄为12.5天胎鼠大脑皮质培养出的神经干细胞数量最多，可分化为神经元、神经胶质细胞及少突胶质细胞。

When E1A mutant containing CR2 domain deletion expressed in ovarian cancer cells that contain wild type Rb, we detected low E2F activity. Also, Bcl-xL deamidation was suppressed and the cells were survived under cisplatin treatment in E1A mutant transfected cells.

故当E1A mutant (CR2 domain deletion)在含wild type Rb的卵巢癌细胞中表现时，我们侦测到E2F活性较低且Bcl-xL去胺基化现象被抑制，这使得细胞对抗癌药物 cisplatin具有些微抗性，存活率上升。

RESULTS: The rates of TNFR1 expressed in decidual glandular epithelia cells, stromal cells and blood vessel endothelial cells of abortion group were 77.4%, 93.5% and 67.7% respectively, and in the control group were 96.7%, 70.0% and 23.3% respectively.

TNFR1在蜕膜间质及血管内皮细胞中表达水平的升高及其在腺体上皮细胞中表达水平的下降，可能与不明原因早期自然流产有关。

The place and time of cell proliferation are just in accordance with those of the appearances of stem cells and stem-cell blastemas, which means that cells in those areas mentioned above dedifferentiate forming stem cells which proliferate too.

这些分裂细胞在位置和时间上与干细胞及干细胞原基的出现一一对应，表明上述几处发生了细胞的脱分化，形成了干细胞，进行着细胞增殖。

It is well known that retinal pigment epithelial cells are predominant proliferative cells in PVR which is an excessively wound healing response occurring after RPE cell wounding. The activated RPE cells migrate from their normal, sessile state to vitreous and onto both surfaces of the retina, in which they begin to dedifferentiate, migrate, proliferate, change phenotype and secrete ECM.

既往研究证明视网膜色素上皮(retinal pigmentepithelial，RPE)细胞是参与PVR的主要细胞，PVR是视网膜脱离后RPE细胞损伤的一种自我修复过程，是一种过度的眼内创伤修复反应；RPE细胞在正常位置上处于静止状态，而在某些病理条件下，如视网膜裂孔形成和视网膜脱离后，RPE细胞脱离原位，开始去分化、移行、增生、发生表型转化并分泌胶原等ECM，最终在视网膜前后表面和玻璃体内形成具有收缩能力的增生膜，造成牵拉性视网膜脱离。

Methods The IL17 cDNA and Thy1.1 fulllength cDNA were subcloned into MIT(MSCVIRESThy1.1) retrovirus vector, and the siRNAIL17, U6 promoter and Thy1.1 fulllength cDNA were also inserted into retrovirus vector of pMNDBANSHEE.The recombined vectors were transfected 293 packaging cells by DNA calcium phosphate coprecipitation. Virus supernatant which infected preactivated spleen cells from NOD/BDC mice was collected. After incubation, the IL17 expression in diabetogenic T cells was detected.

利用基因工程和细胞克隆技术分别将IL17的cDNA插入MSCVIRESThy1.1逆转录病毒载体，将Thy1.1、U6增强子(U6 promoter)基因和IL17的siRNA cDNA插入逆转录病毒载体pMNDBANSHEE，采用磷酸钙沉淀法将重组载体转染293包装细胞；收集病毒上清，转染预先活化的NOD/BDC小鼠脾细胞，测定致糖尿病性T细胞的IL17和siRNAIL17的表达。

Flow cytometry results showed that: 1） When cells were treated with Diallyl trisulfide at the concentration of 10 μg/mL for 12 h and 24 h the percentage of G0/G1 phase cells was decreased and that of G2/M phase cells was significantly increased compared with those in the control group.2） Diallyl trisulfide down regulated Bcl expression while up-regulated bax expression.

结果：MTT显示大蒜素能抑制人结肠癌 HT29细胞的增殖，并具有时间-剂量依赖性；流式细胞术测定结果显示10 μg/ml大蒜素可明显诱导HT29细胞凋亡，并将细胞周期阻滞在G2/M期；可降低癌基因蛋白Bcl表达，而促进Bax表达。

Cells cultured with method 2 became smaller after 2 hours, formed dipolar or multipolar body cells, and most of cells were died after 48 hours.

方法2诱导2 h即可见细胞体积变小，形成双极或多极的细胞体，可持续诱导48 h，后大部分细胞浮起死亡。

Immunocytochemical staining was used to identify sarcomeric actin and intercalated disc-like structure when the cells were cultured for additional 1 week, 2 or 3 weeks, respectively. RESULTS: Sarcomeric actin positive cells were observed in 1 week, 2 or 3 weeks after 5-aza treatment. Some cells stained positive for connexin43 at 1 week and 2 weeks after 5-aza treatment, with brown pellet located dispersively around nucleus.

结果： 5-aza处理后1周、2周、3周均可见横纹肌肌动蛋白染色阳性细胞。5-aza处理后1周及2周连结蛋白43在部分细胞上表达：呈核周散在分布的棕黄色颗粒，5-aza处理后3周连结蛋白43 在细胞密度大的区域呈核周聚集成线形分布的棕黄色颗粒，个别细胞间可见此结构，类似正常心肌的闰盘结构。

To investigate the expression, location and function, long distance PCR was used to expand the targets containing open reading frame. The expressive vectors of prokaryotic and eukaryotic cells were constructed after product purification and connecting with vectors. The prokayotic vectors were induced to express the protein by IPTG and the protein was seen by denaturalization PAG electrophoresis and dyeing. The eukayotic expressive vectors were transfected into culture cells and the protein was found in the nulceolus under the observation of the confocal microscope. The transfected cells were chosen by the geneticin (G418). The cell cyele was examined by flow cytometer and the cell numbers were reduced obviously in the stage of G〓-G〓 and G〓-M, but increased distinguishably in the S stage.

为研究磷酸化应激诱导蛋白的表达、定位和功能，采用了长距离聚合酶链反应扩增含有开放阅读框架的靶片段，经产物纯化、与载体连接，分别构建了STIP1基因的原核表达载体和真核表达载体；IPTG诱导原核表达载体，变性聚丙酰胺凝胶电泳与染色后，可见相应大小的蛋白质表达；STIP1基因的真核表达载体转染细胞系后，共聚焦显微镜下观察STIP1蛋白主要分布于细胞核内；用药物筛选STIP1基因转染的细胞后，流式细胞仪检测细胞周期，可见G〓-G〓期和G〓-M期的细胞明显降低，S期细胞明显增多。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。