查询词典 caliciform cells

Methods: The cells were treated with the different concentrations of Cobaltous chloride. The lactic acid dehydrogenase from the supernatant of the cells was detected by a biochemical analysis. The cells were co-transfected with a pGL2-eNOS-p vector while stimulated with Cobaltous chloride at the different concentrations and disposing time, and the transcription activity of human eNOS promoter was determined through using a double luciferase reporter gene system.

用含不同浓度氯化钴的培养基培养细胞，检测细胞培养上清中的乳酸脱氢酶含量；将已构建的pGL2-eNOS-p质粒转染HUVEC-12细胞，利用双荧光素酶报告基因技术检测在不同浓度氯化钴和不同作用时间下的eNOS启动子转录活性。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

Methods Chondrocytes were obtained from the costicartilage of rat and were cultured on the porous HA blocks, 3 mm×3 mm×4 mm size, for three and seven days. Scanning electron micrograph was taken to show whether the cells grew outside and inside the pore of HA block. The cells cultured on tiny glass sheet for 2 days were used to prove where the cells come from by in situ hybridization technique with α1cDNA probe.

采取成年雄性SD大鼠肋软骨细胞，以3 mm×3 mm×4 mm大小的块状多孔HA为载体培养软骨细胞；培养3天和7天后通过扫描电镜观察HA表面和中矢横断面孔隙中有无细胞生长，同时将培养2天的细胞爬片以α1cDNA片段为探针进行原位杂交，求证培养的细胞是否软骨细胞。

The results showed that the surface and cryptal lining cells of the pseudostratified ciliated columnar epithelium and the superficial squamous cells of the metaplastic squamous epithelium both underwent membranous expression of Fas, but the basal cells did not.

为了探讨Fas在鼻咽癌癌细胞凋亡过程中的作用，我们对37例未经治疗的非角化性人体鼻咽癌组织癌细胞Fas表达进行了研究。

At 24 hours after the removal of 5-FU, most of the epithelial cells were increased in number, showing cuboidal and merged into pieces. At 48 hours, only few positive cells could be seen with some of the pseudostratified mucociliary epithelium, with ciliated cells.

氟尿嘧啶去除6h后，气管基底膜上可见少量扁平样细胞。24h后基底膜上细胞数目增加，并逐渐分化呈立方状，连接成片。48h后气管上皮中出现假复层柱状上皮，并可见纤毛细胞。

In addition, mRNA for the cytolytic molecules, perforin and FasL, were detected in the expanded γδ T cells, and the cytotoxicity of γδ T cells against hepatoma SMMC7721 cells was remarkably dependent on the extracellular Ca〓.

MEP激活的γδ T细胞表达穿孔素和FasL等与杀伤作用有关的效应分子，并且γδ T细胞对肝癌细胞株SMMC772l的杀伤作用有显著的〓依赖性。

Results ① The percentage of positive TPOAb and TGAb were 91.4% and 74.4%, respectively in all the AIT patients. 47.6% of the patients had TSH levels within normal range (0.3～5mu/L).② All of the slides had different grades of lymphocytic infiltration. 49.3% had germinal center, 32.8% had Askanazy cells, 26.9% had plasma cells, 22.4% had colloid, and 9% had multinuclear giant cells.③ Lymphocytic infiltration was divided into four degrees. The levels of TSH and TPOAb increased significantly in the extremely heavy lymphocytic infiltration grade than in the others (P.05). There was no relationship between serologic markers and other cytopathologic features.

结果 ①82例患者中TPOAb、TGAb的阳性率分别为91.4%和74.4%，约有半数(47.6%)TSH值位于正常值范围内(0.3～5mu/L)；②所有患者细胞病理学均存在淋巴细胞不同程度的浸润，49.3%可见生发中心，32.8%可见嗜酸性变细胞，26.9%可见浆细胞，22.4%可见胶质，9%可见多核巨细胞；③将淋巴细胞浸润程度分级，分别与TSH、TPOAb及TGAb进行相关性分析，发现仅淋巴细胞极重度浸润者其血清TSH、TPOAb水平较其他组显著升高(P.05)，其他细胞病理学改变与血清学指标之间未发现显著相关性。

In addition, the TIG-310 was fused to GFP and expressed in L cells and COS-7 cells. It seems that the TIG-310 gene may be a cytoplastic protein, for the fluorescence is mainly found in the cytoplasm of the transfected cells.

由于肠粘膜含有多种细胞，其功能又各不相同，我们用原位杂交方法分析肠粘膜中表达TIG-310的细胞，发现该基因主要表达在肠粘膜的上皮细胞。

Result In the HepG2 cells transfected by pSliencer 3.1-H1 neo-shTERT, expressions of hTERT, Bcl-2 and mitochondrial cyt C were significantly down-regulated, while Bax and cytoplastic cyt C were obviously up-regulated, and active caspase-9 was found in addition to procaspase-9 compared with that in negative control cells and untransfected cells.

结果 与未转染细胞和对照细胞相比，转染细胞hTERT、Bcl-2和线粒体cyt C表达明显减少，Bax和胞质cyt C表达明显增加；未转染细胞及对照细胞仅见约46kD的前caspase-9条带，转染细胞可见46kD前caspase-9条带和35kD caspase-9 p35亚基条带。

Its functions can be divided into three subsets: T helper cells, inhibition of cytotoxic T cells and T cells.

按其功能可分为三个亚群：辅助性T细胞、抑制性T细胞和细胞毒性T细胞。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。