查询词典 caliciform cells

Methods: CD34(superscript +) cells from fetal liver were isolated with a magnetic cell sorting kit and were cultured. Cells pretreated with or without protein kinase C inhibitor chelerythrine chloride (3 μmol/L) were induced by 5 ×10^(-7)mol/L tretinoin for 24 h, and then incubated in serum-free medium. Expressions of genes in treated cells were assayed by Western blotting and RT-PCR.

采用免疫磁珠法分离人胎肝CD34细胞，培养4d后，蛋白激酶C特异抑制剂chelerythrine chloride(3μmol/L)处理24h，再加入维A酸处理24h5×10^(-7mol/L，无血清培养基培养5d，Western印迹和半定量RT-PCR法分析维A酸处理前后神经特异基因表达。

MSC can be obtained by percoll fluid and density gradient centrifugation, this cells kept proliferation and differentiation ability in vitro; MSC can differentiate into chondroblast by lightly centrifugation and chemical definition medium, after one generation, the differential cells kept the phentype of chondroblast, they are a kind of ideal seed-cells for cartilage tissue engineering.

经percoll液密度梯度离心法能获得纯度较高的人骨髓间充质干细胞，这种细胞在体外培养体系中保持旺盛的增殖能力；经轻度离心和化学限定培养基法可诱导骨髓间充质干细胞向成软骨方向分化，增殖旺盛，可传一代培养，是软骨组织工程理想的种子细胞。

Bone marrow containing hematopoietic stem cells has already been known , theses another group stem cells exists in bone marrow named mesenchymal stem cells. MSCs retain the potentiality of differentiating into osteoblast, chondroblast, adipoblast, tendonblast, myoblast, cardiomyoblast, neuron, etc , which displays a bright prosperity of its use in tissue enigeering, wound healing , cell transplatation and gene therapy. MSCs can be obtained and expanded easily compared to ESC without any problems of morality , law and ethic , immunology.

最早在130年前，德国病理学家Cohnheim在研究创口愈合时就推测在创口愈合过程中出现的部分细胞可能来自骨髓。20世纪70年代，Friendenstein发现一小部分骨髓中贴壁最牢固的细胞经数此传代后形成独特的纺锤形外观，具有形成成骨细胞或软骨细胞的能力。80年代以来，许多研究小组进一步证实了Friendenstein分离到的这些细胞是多能的，在体外特定诱导条件下，可以分化为成骨细胞、软骨细胞、脂肪细胞、成肌细胞、神经元等，被称为骨髓间充质干细胞(Bone MarrowMesenchymal Stem Cells，MSCs)。

In resent year, several in vitro models for committed differentiation of ES cells have been described which give rise to a variety of cell types, including hematopoietic, neuronal, chondroitic, osteocytic, endothelial, myogenic, cardiac and smoothes muscle cells, but the committed differentiation into epidermal stem cells have not been reported.

ES细胞为研究基因功能、胚胎发生和个体发育，及组织细胞分化机理提供了理想的模型。近年来的研究表明，适当条件下，ES细胞可定向分化为三个胚层的各种类型的细胞，其中包括向神经元、造血细胞、成骨细胞、心肌细胞、血管内皮、软骨细胞、平滑肌特方向的定向分化。

In resent year, several in vitro models for committed differentiation of ES cells have been described which give rise to a variety of cell types, including hematopoietic, neuronal, chondroitic, osteocytic, endothelial, myogenic, cardiac and smoothes muscle cells, but the committed differentiation into epidermal stem cells have not been reported.

但定向分化为表皮干细胞至今未见报道。

It is very similar between the secretion of rat pancreatic β cells and gonadotropes in general. Their secretions is also very similar to those observed in other endocrine cells, such as adrenal chromaffin cells. This study further proved that the three phasic process of secretion are ubiquitous.

虽然具体的分泌特性有所不同，但大鼠胰腺β细胞与垂体促性腺激素细胞的分泌情况在总体上很相似，这与前人在其他细胞上的发现也非常相似，本文的实验进一步证实了这种三个阶段的分泌过程和机制在不同的细胞中是普遍存在的。

Methods:The effect of chrysin on HIV1 infection and replication in vitro were detected by cell fusion of MT2 cells and HIV1ⅢB/H9 cells labeled calceinAM and the inhibition study of syncytia formation and p24 production monitored in culture supernatant of MT2 cells infected by HIV1ⅢB, respectively. Flurescence conjugated monoclonal antibodies and flow cytometry were used to detect the expression of CD69 by activated CD4+T in response to PHA.

以CR对CalceinAM标记的慢性感染HIV1ⅢB的H9细胞（H9/HIV1ⅢB）和MT2细胞的融合、对HIV1诱导的MT2细胞形成合胞体及对感染HIV1的MT2细胞产生p24抗原的影响，探讨其抗HIV1活性；以PHA刺激人外周血单个核细胞，利用流式细胞术，检测CD4+T细胞CD69表达的百分率，分析CR对免疫活化的影响。

After propagation, GTH cells evacuated large number of secretory granules, TSH cells appeared vacuoles, rough endoplasmic reticulum in STH cells were circumnuclear.

繁殖后，GTH细胞中的分泌颗粒出现排空；TSH细胞有空泡；STH细胞中的粗面内质网呈现围核环状排列。

Xenopus laevis Tadpole (1) Xenopus laevis Tadpole was capable of lens regeneration, which originated from the outer cornea.(2)The days of lens regeneration of Xenopus laevis Tadpole was earlier than Bufo Raddei Strauch Tadpole, The rate of regeneration of Xenopus laevis Tadpole was higher than Bufo Raddei Strauch Tadpole.(3)During Xenopus laevis lens regeneration the outer cornea cells proliferated, in the elongating cells the mitochondria became complicated and in the columnar cells the cisternal dilating of endoplasmic reticulum was ubiquity.

非洲爪蟾蝌蚪(1)再生晶状体来源于外角膜；(2)晶状体再生较花背蟾蜍蝌蚪晶状体再生时期早，再生率高；(3)电镜观察发现再生前期外角膜增殖，观察到晶状体上皮细胞和伸长细胞(进一步发育为晶状体纤维细胞)，伸长细胞中线粒体形状各异随着进一步的分化，细胞器逐渐消失；分化过程中晶状体上皮细胞粗面内质网增多，并且内质网扩张普遍存在。

The larvae should be fed Isochrysis zhanjianggensis (5.0×l0^4 cells/mL), supplemented with Nitzschia closterium (3.0×10^4 cells/mL), and Chlorella sp.(10.0×10^4 cells/mL).

4幼虫培育期间的饵料以湛江等边金藻为主（5.0万个／mL）并辅以新月菱形藻（3.0万个／mL）、扁藻（0.4万个／mL）、小球藻（10.0万个／mL）。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。