查询词典 caliciform cells

The survival rates of CEM cells cultured with cis-diamminedichicloroplatinum added 24 h later were higher than that cultured with hTERT ASODN and DDP added 24 h later. The survival rates of CEM cells cultured with DDP were similar with that cultured with hTERT SOND and DDP. In morphological observation of apoptotic cells using Giemsa staining, cells treated with DDP or DDP combined with hTERT ASODN ro SODN at 48 h, displayed classic apoptotic changes. Apoptosis rates of CEM cells treated with DDP for 48 h after 24 h of exposure to ASODN significantly increased. There was significant difference in the percentage of apoptotic cells of CEM cells between hTERT ASODN plus DDP and SODN plus DDP or DDP alone, respectively.

结果： hTERT ASODN作用于CEM细胞24 h再加入柔红霉素、长春新碱、足叶乙甙，对细胞生长的抑制分别与单用柔红霉素、长春新碱、足叶乙甙及hTERT正义寡核苷酸联合柔红霉素、长春新碱、足叶乙甙组相比，统计学上无显著差异(P＞0.05)。hTERT反义核酸作用于CEM细胞24 h加入顺铂，再共同作用48 h，CEM活细胞均数为2.318×108 cells/L，与单用顺铂组(3.250×108 cells/L)及hTERT正义核酸联用顺铂组(3.175×108 cells/L)相比，对细胞抑制明显增强(P＜0.05)。hTERT ASODN作用于CEM细胞24 h再加入顺铂作用48 h，细胞出现典型的凋亡形态学改变。hTERT ASODN与2.5 μmol/L顺铂联合作用于CEM细胞48 h的凋亡细胞百分率(19.47%)分别同SODN与顺铂联合作用组(6.97%)、单用顺铂作用组(6.02%)进行比较有显著差异(P＜0.01)。

Methods Hepatoma carcinoma cells and pulmonary carcinoma cells were cultured in vitro, Mononuclear cells in healthy person's peripheral blood were collected. PBMCs were mixed with liver cancer and lung cancer cells by proportion, and enriched tumor cells by superparamagnetic nanoparticle antibody complex, lung cancer specificness was identified by fluorescence nanocrystals bioprobe. We smeared tumor cells on glass slide and identified pulmonary carcinoma cells specfficity by fluorescence nanocrystals bioprobe.

体外培养人肝癌细胞和肺癌细胞，采集健康人外周血单个核细胞，将PBMC与肝癌细胞和肺癌细胞按比例混合，通过磁粒抗体复合物富集癌细胞，用荧光纳米晶探针进行肺癌特异性鉴定。

At postnatal 1st week immunopositive reaction of NGF was detected mainly in sustentacular cells and the spermatogonia also showed positive staining. NGF positive staining in the testes was observed in interstitial cells, spermatogenetic cells, sustentacular cells and Leydig cells at 3rd week. After the postnatal 5th week, NGF-positive immunostaining was also detected in intersitial cells and spermatogenetic cells, but the intensity of reaction was weaker than that at 1st and 3rd weeks.

免疫组化定位分析显示：睾丸组织的神经生长因子蛋白表达于小鼠出生后的各个时期内，1周龄睾丸组织免疫阳性反应主要位于支持细胞，精原细胞也有着色；3周龄睾丸组织的间质细胞、各级生精细胞、支持细胞、管周肌样细胞表达均呈现阳性；5周后的睾丸组织内神经生长因子呈低水平表达，主要表达于间质细胞和生精细胞内。

RESULTS: APC promoter 1A was methylated in NCI-H460 cells, and unmethylated in NCI-H446 and SPC-A1 cells. Hypermethylation was detected in all 5 CpG islands (687, 707, 714, 719, 726) of APC promoter 1A in NCI-H460 cells. The expression of APC in NCI-H460 cells was decreased by 26.04% of that in NCI-H446 cells and by 32.36% of that in SPCA1 cells. After treatment of 1, 5, 10, 15μmol/L 5-aza-dC, the expression of APC promoter 1A in NCI-H460 cells was enhanced by 4.59, 5.78, 9.58, 5.98 folds, respectively.

结果：SPC-A1和NCI-H446细胞APC甲基化阴性，NCI-H460细胞APC甲基化阳性；甲基化芯片检测Ncl-H460细胞在APC启动子1A 5个CpG位点均存在甲基化(687、707、714、719、726)，SPC-A1和NCI-H446甲基化阴性，荧光定量结果NCI-H460的APC转录较SPC-A1和NCI-H446有明显的下降，仅为二者平均的30.04%；经5-aza-dC脱甲基化作用后，NCI-H460细胞的APC表达增加了约5~10倍，其中10μmol/L浓度作用下，APC表达增加最多。

Type II cells were mainly distributed in layer V of cortex. It appeared that these cells receive the CRF immunostaining fibers terminals afferent from other cells rather than synthesize CRF by themselves. It is probable that type II cells receive afferent fibers from both extracortex and type I cells within layers II -III cortex. Considered that Type I cells morphologically are inhibitory interneuron, we presumed that type I in layer II -III could inhibit activity of type II cells in layer V.

CRF阳性Ⅱ型细胞主要分布在皮层第Ⅴ层，形态学特征显示这类细胞更像是接收来自其它神经元的CRF纤维投射而非自身分泌CRF，证据显示Ⅱ-Ⅲ层CRF神经元纤维可进入第Ⅴ层，考虑到皮层的CRF神经元形态上为抑制性中间神经元，这些结果提示Ⅱ-Ⅲ层CRF神经元能够抑制第Ⅴ层神经元的活动。

Results There was a similar distributive pattern of Neul, PPCA and β-gal in the inner ear. Neul intense staining was observed in the cochlear spiral ganglion cells, spiral limbus, spiral ligament, vestibular ganglion cells, cristae, maculae hair cells, and weak staining in inner hair cells, outer hair cells, supplying cells of the organ of Corti and stria vascularis. The intense staining of PPCA and β-gal were observed in the spiral ganglion and vestibular ganglion cells, and weak staining in the spiral limbus, spiral ligament, stria vascularis and organ of Corti. The inner ear exhibited no staining when Neul, PPCA and β-gal were deficient, respectively.

Neul最强的染色主要在螺旋神经节细胞、螺旋韧带、螺旋缘、前庭神经节细胞及壶腹嵴、球囊和椭园囊感觉毛细胞，较弱的染色分布于血管纹和Corti器内、外毛细胞及支持细胞；PPCA和β-gal在螺旋神经节和前庭神经节细胞有较强的染色，血管纹、螺旋韧带、螺旋缘和Corti器内、外毛细胞及支持细胞呈较弱的染色反应；各自酶缺乏时内耳免疫染色消失。

Results (1) Cells in frontal recess: The existence of terminal cells was 45.5% in frontal sinusitis group and 23.7% in, control; anterior ethmoid cell 31.8% and 15.3%; agger nasi cells 28.8% and 13.5%.(2) Cells in frontal sinus: for perifrontal cells there were 42.4% and 22%, respectively; superaorbital cells 33.3% and 25.4%; intersinus septal cells 27.3% and 20%.

①额隐窝内出现的相关气房（无额窦炎组出现率／额窦炎组出现率）：终末气房(23.7%/45.5%)，前筛气房(15.3%/31.8%)，鼻丘气房(13.5%/28.8%)；②额窦内出现的相关气房：额气房(22%/42.4%)，眶上气房(25.4%/33.3%)，额窦中隔气房(20%/27.3%)。

Results were shown as followings:(1) Sodium selenite at 0～2.5 μmol/L significantly increased the antioxidative capacity of L-02 cells without having remarkable impact on SMMC-7721 cells;(2) Sodium selenite at concentrations above significantly increased telomerase activity, hTERT gene expression and telomere length of L-02 cells without significant impact on SMMC-7721 cells;(3) Sodium selenite at higher concentrations (larger than 5 μmol/L) resulted in peroxidation of L-02 cells, while scutellarin significantly counteracted its effect;(4) Selenium-rich amino acids from silkworm pupas in the range of 0.5～2.5 μmol/L Se significantly inhibited SMMC-7721 cell growth, induced apoptosis and cell cycle change, and the generation of reactive oxygen species. In contrast, sodium selenite and selenomethionine only had weak impact on them at the same concentrations;(5) A new selenium-containing protein was found from selenium-rich silkworm pupas, which is worthy to be study further;(6) An expression vector containing ansense RNA of hTERT gene were constructed and used to transfect SMMC-7721 cells. They were observed to inhibit hepatoma cells.

结果如下：（1）0～2.5μmol/L亚硒酸钠显著性增强L-02细胞的抗氧化能力；而对SMMC-7721细胞的作用不显著；（2）该浓度硒显著性提高L-02细胞端粒酶活性、增强hTERT基因表达和延长细胞端粒长度；而对SMMC-7721细胞的作用均不显著；（3）高浓度硒（5μmol/L以上）显著性抑制L-02细胞生长、致细胞过氧化，灯盏花素能拮抗硒所致过氧化、降低硒毒性；（4）0.5～2.5μmol/L富硒蚕蛹氨基酸显著性抑制肝癌细胞SMMC-7721生长、导致细胞凋亡和周期改变、诱导细胞产生活性氧，同浓度亚硒酸钠和硒代蛋氨酸对其抑制不显著；（5）富硒蚕蛹蛋白经分离纯化和鉴定后发现存在一新含硒蛋白，其结构和功能有待研究；（6）通过已有的含hTERT基因质粒，成功构建hTERT反义RNA表达质粒，转染SMMC-7721细胞后对其生长具有抑制作用。

The morphology and structure of reconstructed tissue was detected by microscope and scanning electron microscope.Results:(1) Compared with the control group, the cellular proportion of laminin group increased in 62 ~M phase, and decreased in Go~Gi phase significantly. As shown by the microscope, the cells of control group were in low density. The cells in mass connected tightly. The microfilament appeared reticular formation. The nucleus were the same in size. The cells of laminin group were in high density and put out so many lamellipodia, filopodia, which connected with the surrounding cells. The microfilament increased, elongated, and changed from reticulodromous to sarciniform, which reached to the pseudopods. The nucleus were different in size .(2) As shown by the inverted microscope and the cell growth curve, comparing with the controlgroup, cells of each test group increased evidently. The cellular proportion of each test group increased in S phase and G2 ~M phase, and decreased in Go~Gi phase significantly, but there was no considerable interations between LN and EGF;(3) As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemets membrane closely, which was similar to the natural tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately.

结果：(1)层粘连蛋白组处于G_2～M期细胞比例较对照组显著提高，Go～G_1期细胞比例显著下降，提示层粘连蛋白促进内皮细胞DNA合成，及细胞分裂增殖；光镜下，对照组细胞分布成团状，细胞密度较低，细胞间连接紧密，细胞内微丝结成网状，细胞核大小一致；与对照组相比，层粘连蛋白组细胞生长旺盛，细胞密度高，向周边伸出大量板状及丝状伪足，细胞内微丝增多、拉长、集结成束，伸入伪足中，细胞核形状大小不一致；(2)倒置显微镜观察及细胞生长曲线显示，各组细胞数目随时间增加而明显增多，各实验组较对照组增生显著，EGF和LN联合应用组各时间点细胞数目最高；实验组处于S期和G_2～M期细胞数目增加，Go～G_1期细胞数目减少；提示EGF、LN单独及联合应用均可促进细胞增殖，但尚不能认为二者有交互作用；(3)倒置显微镜下，组织培养的猫角膜内皮细胞排列成密集的单层，细胞间连接紧密；组织学观察发现，培养的猫角膜内皮细胞形成完整的内皮层，贴附于脱水基质的后弹力膜上，与正常的角膜内皮组织结构相似；扫描电镜下，组织培养的猫角膜内皮细胞间紧密镶嵌排列，可见某些细胞呈近似六边形排列，细胞大小不甚一致，胞核清晰。

Results Compared with that in the NP tissues, the number of α-SMA positive cells increased significantly in BPH tissue, and the number of vimentin positive cells increased moderately in the stroma and prominently surrounding the acinus and in the basal layer. In BPH tissue, the myosin and ERα staining signal was lost in the stromal cells surrounding the acinus, and the positive staining cells gathered into bunches in the stroma far away from the acinus, while the positive cells were sporadically distributed in the NP tissue. The PCNA positive cells increased moderately in the stroma and increased significantly in the basal layer.

结果 与NP相比在BPH中，α-SMA阳性染色细胞显著增加；波形蛋白在间质中阳性染色细胞有所增加，在腺泡基底层及临近腺泡外层间质中阳性染色细胞明显增加；在临近腺泡外的数层间质细胞中肌球蛋白和ERα由部分阳性变为完全阴性染色，而在远离腺泡的间质中其阳性染色细胞由散在斑块状分布变为簇状密集排列；PCNA在间质中阳性染色细胞有所增加，在基底细胞层中阳性染色细胞显著增加。

The shaping method of noncircular part and the tool holder's radial motion characters in noncircular turning process are discussed in detail in the thesis.

论文详细研究了非圆零件的成型方法和加工过程中刀架的径向运动规律。

I have not really liked him,I do not like his this kind of disposition.

我没有真的喜欢他，我不喜欢他的这种性格。