查询词典 caliciform cells

Furthermore, preliminary work also performed to examine whether PI3K/AKT signal transduction pathway was activated in the process of refractory leukemia development. Materials and methods An immortalized human bone marrow stromal cell line, HS-5, was introduced to establish a bi-phase culture system for the cultivation of B-lineage precursor leukemia cells. ELISA and RT-PCR were used to investigate the expression of VEGF and its receptors in the leukemia cell lines and primary childhood leukemia cells in different treated groups. Flow cytometory method and immunofluorescent staining were employed to examine the apoptosis signals both in the VP16 treated and untreated leukemia cells. Western blot was utilized to explore the PI3K/AKT activated status in the drug induced or uninduced leukemia cells and lymphocytes from healthy donors.

材料和方法使用来源于人类骨髓基质细胞的细胞株HS-5作为滋养层细胞进行急性淋巴细胞性白血病细胞的体外培养，通过细胞生物学和免疫学方法评估培养体系并鉴定出难治性白血病细胞克隆；以ELISA和RT-PCR方法检测急性白血病细胞株和患儿白血病细胞VEGF及其受体的表达，了解不同治疗阶段VEGF及其受体的表达状况，并结合临床指标进行分析，明确VEGF及其受体在白血病发生过程中的作用；流式细胞仪和免疫荧光染色法对正常健康儿童、初发白血病患儿、复发白血病患儿及缓解后患儿进行凋亡因子检测和分析，初步阐明难治性白血病抗凋亡形成的原因；蛋白印记分析检测PI3K/AKT信号传导通路在健康儿童、初发白血病和复发白血病患儿的表达，初步了解难治性白血病形成的分子生物学机制。

Methods C3b sensitized yeast cells and C3b unsensitized yeast cells were used to detect C3b receptors and immune complex on red cells,method of xanthine oxidase was applied to detect SOD activity in red cells.

实验利用低碘饲料和碘化钾去离子水复制了不同碘营养状态的大鼠，采用C3b致敏和未致敏的酵母菌及黄嘌呤氧化酶法分别检测红细胞膜上的C3b受体、免疫复合物及红细胞内SOD的活性。

ABSTRACT Objective To discuss the clinical pathologic features and differential diagnosis of mixed germ cell sex cord stromal tumorwith malignant variant of germ cell tumor Methods The clinical pathologic datum and immunophenotype were studied in the case of ovary MGCSCST with malignant variance of germ cell tumor Results The patient's genital anatomy and female phenotype were normal,karotypes was 46xx with uterogestation The tumor tissue consisted of mixed germ cells and sex cord stromal cells with evident malignancy of mixed germ cells There was no atypical structure of gonadoblastoma in tumor tissue Conclusion MGCSCSST has complex morphosis,thus,to prevent misdiagnosis,more samples should be collected and more slices be cut The patient may have prognosis malo when there are high malignant variants of mixed germ cells in tumor tissue

目的 探讨混合性生殖细胞性索间质肿瘤(mixed germ cell sex cord stromal tumor，MGCSCST)伴恶性生殖细胞肿瘤变异型的临床病理特点及鉴别诊断。方法卵巢MGCSCST伴恶性生殖细胞肿瘤变异型的病例进行临床病理特征和免疫表型分析。结果患者生殖器的解剖结构和女性表型正常，染色体组型是46xx，足月妊娠。肿瘤组织由混合性生殖细胞和性索间质肿瘤构成，混合性生殖细胞有显著的恶性特征。瘤组织中没有典型的性腺母细胞瘤结构。结论 MGCSCST有复杂的形态结构，因此更多的取材和切片可以防止误诊，当肿瘤组织中的混合性生殖细胞有高度恶性变异时，患者预后不良。卵巢肿瘤；生殖细胞性索间质肿瘤；免疫组织化学

Results IL-6R immunoreactive cells were observed in the anterior of optical area,the ventromedial nucleus,the periventricular nucleus and arcurate nucleus of the hypothalamus.The strongly stained cells are concentrated in the granule cell layer of the dentdate gyrus and the pyramidal cells of the CA1-CA2 fields of the hippocampus.IL-6R positive cells are also detected in the cortex, the ventromedial nucleus of the thalamus,bed nu stria,olfaltory tubercle.

结果 IL-6R 免疫反应性细胞主要分布在下丘脑的视前区、腹内侧核、室旁核和弓状核；在海马， IL-6R 阳性细胞呈强阳性，密集分布于齿状回颗粒细胞层和 CA1～ CA2锥体细胞层；在大脑皮层、嗅结节、丘脑腹内侧核、终纹床核等处也有 IL-6R 阳性细胞。

The cell cycle and apoptosis and the expression of P-pg, bcl-2, P53 in the MG63 and MG63/R cell lines were analyzed by flow cytometry. Results The resistance index of the MG63/R cells to cisplatin was 83.557±4.841. The cells also had resistance to doxorubicin, vincristin, methotrexate and cyclophosphamide. Disordered structure of the MG63/R cells was observed through microscopy. The cells appeared in triangle, polygon and polynucleation. The increase of granular endoplasmic reticulums and apophyses was observed through transmission electron microscopy. The proliferation ability of MG63/R increased significantly, with a low apoptosis index.

结果 经顺铂186d的诱导，建立了MG63/R，其对顺铂的耐药指数为83.557±4.841，对阿霉素、长春新碱、氨甲基蝶吟、环磷酰氨亦产生不同程度的交叉耐药；光镜观察可见MG63/R细胞排列不规则，形态呈三角形、多角形及多核现象；透射电镜显示MG63/R细胞表面突起增加，粗面内质网丰富；细胞周期分析显示细胞增殖能力明显增加而细胞凋亡明显降低；MG63/R细胞 P-pg、bcl-2阳性表达较MG63细胞明显增加，P53表达则明显降低。

Results showed that there were the morphological changes of mitochondria, such as vacuolization, degeneration and cristae swelling or disappearance, in HT-29 cells treated by C2-ceramide for 24 hours. Under the fluorescence microscope, mitochondria of apoptotic cells aggregated and emitted red fluorescence, however, mitochondria of normal cells emitted green fluorescence. The mitochondrial membrane potential began to decrease after 6 hours in HT-29 cells treated with C2-ceramide in time and dose dependent manner.

结果显示C2-神经酰胺作用HT-29细胞24小时，线粒体发生空泡化、变性、嵴肿胀或消失等形态学改变；此时荧光显微镜下可观察到线粒体聚集，发出红色荧光，而正常细胞线粒体发出绿色荧光。C2-神经酰胺作用细胞6小时，线粒体膜电位即开始降低，呈时间-剂量-效应关系。

Electron microscopic findings were: 1. alveolar type I cells were degenerated、 broken-down and desquamated, endothelial cells were swelled, with inter cellular tight junction shortened, alveolar type II cells hyperplastic, basement membrane thinned and deformed; 2. alveolar macrophages and interstitial macrophages were hyperplastic; 3.mast cells were infiltrated and degranulated; 4.electron-dense deposits were present at alveolar wall; 5. myofibroblasts 、fibroblasts、 collagen and basement membrane like material were hyperplastic.

电镜观察可见：(1)I型肺泡上皮细胞变性、崩解和脱落，内皮细胞肿胀，细胞间紧密连接短小，II型肺泡上皮细胞增生，基底膜变薄和破坏；(2)肺泡巨噬细胞、间质巨噬细胞增多；(3)肥大细胞浸润并见脱颗粒现象；(4)肺泡壁电子致密物沉积；(5)肌纤维母细胞、纤维母细胞、胶原原纤维及基底膜样物质增生。

Culture and immunohistochemistry of Schwann cells Schwann cells that are derived from sciatic nerve of Sprague-Dauley rats are successfully isolated, cultured. The Schwann cells are transplanted into the hemisected adult rat spinal cord after purification and condense and enclosed by L I -antibody. Eight weeks later, the experiments of the S- 100 immunohistochemistry indicate that Schwann cells are still alive and array orderly relatively.

本实验的主要研究内容及结果如下： 1、雪旺细胞的培养及免疫组化染色利用新生S-D大鼠的坐骨神经成功地分离、培养获得了纯度较高的雪旺细胞，并将雪旺细胞移植进入大鼠脊髓损伤创面，饲养8周后经过S-100免疫组化ABC染色发现雪旺细胞在大鼠体内成活良好并成簇排列。

The hilling activing of CD3AK+ cells showed significantly higher to K562/VCR cells than to K562 cells, while there was no difference of cytotoxicity between K562 /HHT and K562 cells.

4CD3AK+细胞对K562/VCR细胞的杀伤活性强于对敏感细胞K562，其对K562/HHT细胞的杀伤活性与其对K562细胞的杀伤活性比较，差异无显著性。

METHODS Human IL 6 gene was reconstructed in retrovirus vector and transferred into incasing cells PA317 by lipofectamine mediated method. The clones of the cells transferred with hIL 6 were selected by G418. Targeted NIH3T3 cells were infected with the virus granules secreted from PA317 and also selected by G418. The insertion and expression of hIL 6 gene in NIH3T3 cells were analysed with Southern blot and Northern blot. RESULTS Human IL 6 retrovirus vector (pZIPIL-6) was successfully reconstructed.

利用重组载体构建技术将质粒pUCIL 6 cDNA的目的片段连接于逆转录病毒载体上，并以脂质体介导的方法将重组载体转染包装细胞PA317，以G418筛选克隆细胞，浓缩克隆细胞上清以制备重组病毒液，继之感染NIH3T3细胞后，进行Southern blot和Northern blot分析，检测目的基因在靶细胞的整合与转录水平。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48