查询词典 caliciform cells

Methods MTT Chromatography is adopted to test effects of 5-FU of different concentration and at different time of reaction over growth of Lovo cells; photomechanical method used to observe configuration and characteristics of apoptosis cells; FCM employed to test the percentage of apoptosis cells; electrophoresis and immunized grouping SP applied to test antigen Ki-67 of Lovo cells proliferation and expression of P53 and Fas genetic proteins in relation to apoptosis.

采用四甲基偶氮唑盐显色法，检测不同浓度5－FU作用不同时间对Lovo细胞生长所产生的不同影响；光镜观察凋亡细胞的形态特点；流式细胞术检测凋亡细胞百分率；琼脂凝胶电泳、免疫组化SP法检测 Lovo细胞增殖核抗原Ki－67及凋亡相关基因蛋白p53、Fas表达水平。

Results 5-FU inhibits growth of Lovo cells; it is found that within a limited dosage and period of time, there has been an obvious increase in apoptosis cells under the photomechanical scope; the analysis of Lovo cells by FCM showed that after the induction of 5-FU, the percentage of apoptosis cells has increased; and FCM also displays dosage and time effects. Electrophoresis was obviously in a stripe shape of trapeziform.

结果 5－FU能抑制 Lovo细胞生长，在一定剂量和时间范围内，通过光镜观察，见凋亡细胞明显增多，经5－FU诱导后，用FCM 分析 Lovo细胞，发现凋亡百分率增加并显示剂量和时间效应。

Results While the ES lumen and perisaccular region were infiltrated with mononeuclear-phagocyte at the 3rd day post-vaccination, plasmocyte and lymphatic cells become the predominant infiltrating cells in the ES at the 7th day. The KLH in the ES lumen were phagocytized at the 3-7th day post-vaccination. S-phase cells and IgG positive cells in ES increased markedly in the 3rd day and 7-14 days post-vaccination respectively with similar localization.

结果 内耳受抗原刺激后3 d内淋巴囊囊腔和囊周区域出现单核吞噬细胞浸润，7 d内淋巴囊的浆细胞和淋巴细胞大量增加；3～7 d内淋巴囊囊腔KLH被吞噬，同时S期细胞和IgG阳性细胞逐渐增多，二者的出现部位相似。

We suppose that there is a presently undefined subfraction of stem cell population that exists in a number of tissues beyond embryonic development. We term them postembryonic pluripotent stem cells. They differ from embryonic stem cells in that they gradually lose some differentiation potentials during development and adopt some special phenotypes once within a certain tissue. They can give rise to different kinds of stem cells; therefore, help to maintain balance during development and metabolism. They provide cells not only for the damaged tissues they reside, but also for damaged tissues different from them by migration triggered by proinflammatory cytokines or growth factors.

我们认为在胚胎发育后的多种组织中都存在一类原始干细胞群体，它们和造血干细胞、神经干细胞等多能干细胞不同，后者只能分化为特定胚层的细胞，而这类原始干细胞可分化为不同胚层的组织细胞；另一方面，它们区别于胚胎干细胞，在妊娠期间会逐渐失去部分分化潜能，并且会出现一些特殊表型或者分子标志，我们称其为胚胎后亚全能干细胞，这类干细胞具有下列特性：可分化为不同胚层的细胞，分布于身体的所有组织和器官，具有相同的表型或标志；他们在胚胎期存在于一些或全部的组织和器官中，可分化为不同类型的多能干细胞并在生长发育和新陈代谢中维持这些多能干细胞保持平衡；可以参与机体自我修复和更新，不仅能分化为所在器官的组织特异性细胞，参与器官重塑，也可以在炎症因子或者生长因子的趋化作用下，远处转移修复受损组织。

Methods After the wide type NCI-H446 cells and the three kinds of NCI-H446 cells transfected by three kinds of IL-15 genes respectively （Cmp transfected by the IL-15 maturation peptide gene, Cp transfected by prototypic IL-15 gene, and Csp transfected by modified IL-15 gene whose signal peptide was replaced with the IL-2 signal peptide） were treated by mitomycin, the cells （named MCw, MCmp, MCp, and MCsp, respectively） were used as stimulator cells to stimulate PBMC from healthy volunteers.

野生型NCI-H446细胞和被3种IL-15基因分别转染的3种NCI-H446细胞（Cmp：被IL-15成熟肽基因转染；Cp：被原型IL-15基因转染；Csp：被信号肽换为IL-2信号肽的改型IL-15基因转染），用丝裂霉素处理后（分别名为MCw、MCmp、MCp和MCsp）作为刺激细胞刺激健康志愿者的PBMC。

Fig.1 SHEE cultured on coverslide, the living cells were growing in single layer with rich cytoplasm, the nuclei were uniform in size with a nucleolus ph ×400 Fig.2 SHEE had a nucleus with ellipse shape, large nucleolus and the cytoplasm contained mitochondria and tonofibrilEM ×10 000 Fig.3 SHEE was spherical in shape, with pseudopods attached on petri dish and abundant villi on cell surface SEM ×5 000 Fig.4 Same as in Fig.3, cell attached on petri dish, appeared stellate or polygonal in shape, with abundant pseudopods and cytoplasmic processes. Protrusive nuclear region in central part of the cell had more micro-villi SEM ×5 000 Fig.5 Chromosomes of SHEE cells belonged to diploidy type Giemsa ×1 000 Fig.6 The SHEE cells of stained in dark brown by Ki67 immunohistochemistry were the proliferative cells Immunohistochemistry ×400 Fig.7 In SHEE cell culture, the nucleus stained red or pink by PI was dead cell, the green nucleus was living cell Fluorescent ×400 Fig.8 The cell labeled by TdT was apoptotic cell in which the chromatin of nucleus condensed in block, a pyknotic nucleus in the upper right conner was seen TdT labeled ×400

图1 SHEE培养在盖坡片上，活细胞单层生长，胞浆较丰富，细胞核大小一致，有核仁×400 图2 SHEE培养细胞细胞核椭圆形，核仁较大，胞浆有较丰富的线粒体和张力原纤维EM ×10 000 图3 SHEE细胞呈球状，有伪足贴壁，表面有密集微绒毛SEM ×5 000 图4 同上细胞贴壁，呈星状或多角形，有丰富伪足和胞浆突，核区隆起有较多微绒毛SEM ×5 000 图5 SHEE细胞染色体仍属二倍体Giemsa染色×1 000 图6 SHEE细胞Ki67免疫组织化学染棕黄色为增殖细胞×400 图7 SHEE培养细胞出现死细胞，胞核和胞浆PI染色呈红色或淡红色，蓝色细胞核为活细胞荧光显微镜×400 图8 细胞TdT标记阳性为凋亡细胞，染色质凝集呈块状，右上角有一固缩细胞核TdT标记×400

The follicle cells of growing follicle were composed of small cells, intermediate cells and pyriform cells.

乌梢蛇生长卵泡的滤泡细胞是由小细胞、中间细胞和梨形细胞组成的异形滤泡细胞。

Results After culture and reinfusion in vivo, CD 3 cells, CD 3 CD 8 cells, CD 3, CD 56 cells, cultured cells were significantly elevated, mDC and pDC significantly increased after transfusion, liver function had improved compared with before treatment .

结果 培养结束以及回输体内后， CD 3细胞、CD 3 CD 8细胞、CD 3 CD 8细胞较培养前显著升高，mDC 和pDC 在回输后也明显增高，肝脏功能较治疗前有所好转。

Restoration of ODC expression in the HL60-ODC cells resulted in rescue of rottlerin-induced apoptosis of HL60 cells. Simultaneously, phosphothreonine-ODC of rottlerin-treated HL60-ODC cells was highly elevated comparing with HL60-pcDNA3 cells. Taken together, we suggested that rottlerin, a PKCd inhibitor, might decrease phosphothreonine-ODC to reduce both protein stability and enzyme activity of ornithine decarbooxylase and finally resulted in cell apoptosis.

为了侦测鸟胺酸去羧化是否能够负调节rottlerin所引发的细胞凋亡，我们将具有鸟胺酸去羧化cDNA载体稳定转染至HL60细胞内，此细胞称为HL60-ODC细胞，另转染载体的细胞称为HL60-pcDNA3细胞；实验发现HL60-ODC细胞中的鸟胺酸去羧化蛋白为HL60-pcDNA3细胞与HL60细胞表现之2.5倍，同时HL60-ODC细胞提升鸟胺酸去羧化蛋白表现，能够减少rottlerin造成之细胞凋亡现象，并且此蛋白在HL60-ODC细胞中酥胺酸磷酸化较HL60-pcDNA3细胞表现高。

By culturing human brain microvascular endothelial cells and glioma cells separatedly and simutaneously ,and then analyzing their pre- and post-cultune FasL levels, we study the effect of the grouth of glioma cells upon FasL level of human brain microvascular endothelial cells.

我们通过将人脑毛细血管内皮细胞与胶质瘤细胞分别培养及共同培养，对培养前后人脑毛细血管内皮细胞与胶质瘤细胞FasL表达水平的分析，来探讨胶质瘤细胞的生长对人脑毛细血管内皮细胞FasL表达的影响。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48