查询词典 caliciform cells

Neuroectoderm; neural crest; neural plate; floor plate; neural tube; neuraxis; neurulation; neuroblasts; stem cells; differentiation; ontogeny; morphogenesis; histogenesis; organogenesis; synaptogenesis; gangliogenesis; embryogenesis; axonogenesis; retinogenesis; gliogenesis; glial progenitor cells; oligodendrocyte progenitor cells; retinal progenitor cells; nerve growth factor; neurotrophic factors; trophic factors; growth factors; neural tube defects; anencephaly; spina bifida; neuroblastoma cells; cell migration; neurogenesis; development; developmental stages

神经外胚层；神经脊；神经板；底板；神经管；轴索；神经胚形成；成神经细胞；干细胞；分化；个体发生；形态发生；组织发生；器官发生；突触发生；gangliogenesis；胚胎发生；axonogenesis；retinogenesis；gliogenesis；神经胶质祖细胞；少突神经胶质细胞祖细胞；视网膜祖细胞；神经生长因子；神经营养因子；营养因子；生长因素；神经管故障；无脑；脊柱裂；成神经细胞瘤细胞；细胞迁移；神经发生；发展；发展进程下一页末页共有 23 条记录，本页从第 1 到第 10 条。

Depositing cells were incubated in high-sugar DMEM culture medium containing 15% fetal bovine serum, 100 U/mL penicillin, and 100 U/mL Streptomycin, then cells were seeded in a 25 T culture bottle after cell density was adjusted to 1×109 L-1, and incubated at 37 ℃, 0.05 volume fraction CO2 with saturated humidity. After 72 hours, the culture medium was replaced, and red blood cells and other nonadherent cells were removed. Cells began to passage at 70%-80% confluence.

实验方法：穿刺抽取15 mL脐带血，密度梯度法分离吸取分层界面的白色环形絮状物，其中富含单个核细胞，离心后沉积细胞用含15％胎牛血清、100 U/mL青霉素、100 U/mL链霉素的DMEM高糖培养基悬浮，调整细胞密度为1×109 L-1接种于25 T培养瓶内，放置在37 ℃、体积分数为0.05的CO2饱和湿度培养箱中培养，72 h后更换培养基，去除红细胞及其他未贴壁细胞，至70％~80％融合时传代。

Over the past 28 months, the induced pluripotent stem cells iPS cells, with characteristics identical to those of embryonic stem cells (ES cells directly in vitro reprogrammed from nonembryonic cells and tissues have captured great attentions in both scientific community and general public.

日本和美国研究小组先后用4种基因将小鼠(2006年8月)和人(2007年11～12月)的体细胞在体外重编程为诱导性多潜能干细胞(induced pluripotent stem cells，iPS cells)，此后在短短两年多时间内，iPS 细胞的研究和关注度呈爆炸式增长。

The stem cells may be induced to express at least one characteristic of human intervertebral disc cells, such as fibroblast cells, chondrocyte cells, or notochordal cells, by exposing them to agents and/or environments calculated to induce the desired differentiation.

可通过干细胞暴露于诱导所需分化的试剂和/或环境来诱导它们表达至少一种人椎间盘细胞，如成纤维细胞、软骨细胞或背索细胞的特征。

In this study, the ovarial cancer stem cells were successfully obtained through the suspension culture of the ovarian cancer 0VCAR3 cell line in serum-free media. The stem cells formed spherical colonies, called "ovarispheres", and showed anchorage-independent ability. Furthermore, the bionomics and morphology of the stem cells were observed, and the expression difference of the tumor stem cells marker-ABCG2 between ovarispheres and anchorage-dependent cells was identified by using RT-PCR and Immunocytochemistry methods.

本研究通过无血清悬浮培养人卵巢癌细胞系OVCAR3，获得具有非锚着依赖能力的肿瘤干细胞球，观察其生物学特性及形态学的变化，并采用半定量逆转录—聚合酶链反应及细胞免疫化学的方法鉴定肿瘤干细胞标志ABCG2在贴壁细胞及肿瘤干细胞球中的表达差异。

Results The hEFs were successfully isolated and cultivated from gonadal ridges and dorsal mesenteries of human embryos. They could be passaged beyond the 25th generation. The biologic characteristics of the cells did not change, even in high-passage cells or frozen-thawed cells. The cells expressed prolyl 4-hydroxylase β, but not cytokeratin-4, which was similar to the fibroblasts. The cultured cells expressed bFGF and LIF.

结果：从人胚胎生殖腺嵴和肠背系膜中成功地分离培养出hEFs，该细胞可传25代以上，且经过传代及冻存复苏后生物学特性无改变；hEFs表达脯氨酰4-羟化酶β亚单位，不表达细胞角蛋白4，确定其为成纤维细胞；该成纤维细胞表达bFGF和LIF。

Under scanning electron microscopy, along with the extension of the culture time, the chondrocytes grew and propagated well on the surface. On 3 weeks, the cells adhered to the surface, and extended the pseudopodia, by which the cells were connected each other. The cells secreted matrix, which deposited around the cells and the surface of meniscus. In some area, the cells formed the structure like cartilage lacuna.

扫描电镜观察，随着培养时间延长，细胞在材料表面生长良好，细胞密度增加。3周时，可见细胞黏附在材料表面，有伪足伸出，细胞间通过伪足相互连接，并可见细胞分泌的大量基质沉积于细胞周围和材料表面，部分区域形成类似于软骨陷窝样结构。

The result of AKP test showed that the 3D-colony cells taken the russety colour .The other cells including feeder cells and around cells have not been dyed. It suggests that the 3D-colony cells should have a high activity of AKP, might be in the non-differentiation or low differentiation state.

碱性磷酸激酶的染色结果显示这些细胞克隆呈红褐色，而周围细胞和饲养层细胞未着色或着色很浅，说明这些细胞克隆的内源性碱性磷酸酶活性较高，从而表明这些细胞克隆处于未分化或低分化状态。

We found, GLP-1-IR cells in various guts of rat, pig and foetus of human with distribution scatteredly among the basal portion of intestinal glands. The cell shape corresponded with open type cells, i.e. L-cells. The density of GLP-1-IR cells was different in races, but the distribution regularity of GLP-1-IR cells was same. It showed that a continuous increase from the proximal to the distal portion of small and bowel.

结果发现：在各段肠管都可见到GLP-1-IR细胞；GLP-1-IR细胞散在于肠腺的柱状上皮细胞之间，多位于肠腺的基底部，呈开放型内分泌细胞形态，即L细胞；不同种属问GLP-1-IR细胞密度不同，但分布规律相同，即自小肠和大肠的近端向远端逐渐增大。

The adipose derived stem cells of inbreds train of rat wereinduced to differentiate into schwann-like cells and the cells were injectedinto the acellular nerve allograff.The proliferation or adhersion of these cells on the graft were observed by inverted microscope or ScanningElectronic Microscope. Activity of cells were detected using MTT. 10mmnerve gap of inbreds train of rats in two group were bridged by tissue-engineered peripheral nerve or autogenous nerve, the effect wereappraised by naked eye, recovery rate of sciatic function index,nerve-electrophysiological, histology, Transmission ElectronMicroscopy and quantitative analysis of recovery rate of myelinated fiberpopulations,diameter of myelinated fiber and thickness of myelin sheat.

按照前述方法培养并诱导近交系大鼠脂肪干细胞向类Sehwann分化，将诱导分化的脂肪干细胞悬液注入已制备的去细胞同种异体神经支架管中，倒置显微镜观察细胞生长情况；扫描电镜下观察细胞在材料上附着情况；四唑盐比色试验测定细胞活性；取两组近交系大鼠，分别用组织工程化神经和自体神经桥接10mm神经缺损，通过大体观察、坐骨神经功能指数恢复率的测定、神经电生理的测定、组织学光镜观察、透射电镜观察、再生有髓神经纤维计数恢复率、神经纤维直径恢复率、髓鞘厚度恢复率的测定等指标评价实验效果。

The shaping method of noncircular part and the tool holder's radial motion characters in noncircular turning process are discussed in detail in the thesis.

论文详细研究了非圆零件的成型方法和加工过程中刀架的径向运动规律。

I have not really liked him,I do not like his this kind of disposition.

我没有真的喜欢他，我不喜欢他的这种性格。