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Using this model three competence-deficient pneumococci, which was identified by PCR and transformation experiment, were constructed. It is found different strain has different bacterial optimal density for being induced into competence. The optimal density for strains 1, 2 and 22 was 0.1, 0.08and 0.07 respectively. The optimal condition for transformation is c plus y culture at pH8.0. In c plus y culture at pH6.8, which restrained pneumococci forming transformation naturally, pneumococci could be induced to form transformation by CSP.It is concluded that we could induce different S.pneumoniae by CSP into competence in their optimal density in laboratory. 2. It was discussed the relationship between pneumococcal transformation formation and its opportunistic pathogenesis. Comparing their virulence with that of parental strains by challenging mice intraperitoneally and adhering to ECV-304 cell respectively.

结果表明不同肺炎链球菌株的转化最适菌密度不同,1、2和22号菌株的最适转化密度分别为0.1、0.08和0.07;用于转化的CTM培养基最适为pH 8.0的C+Y培养基;在自然转化下不能诱导转化发生的pH 6.8的C+Y培养基中,使用CSP诱导,也能促使细菌发生转化,只是转化效率较低;结论:实验室条件下,不同细菌在一定菌密度时可利用CSP诱导肺炎链球菌成为感受态发生转化。2、为探讨肺炎链球菌转化对毒力表达的影响,本研究分别通过体外粘附人脐静脉血管内皮细胞ECV-304和体内小鼠毒力实验观察1、2和22号肺炎链球菌和其转化缺陷菌株1t、2t和22t毒力的差异。

Trumpets staggers and grabs for his burning eyes. C-Note pounces, delivering several furious punches before Trumpets collapses to the floor. C-Note is relentless as he stomps on Trumpets again and again.

Trumpets捂着炙伤的双眼,蹒跚而行。C-Note猛了上去,对Trumpets来了几下老拳,Trumpets倒在了地板上。C-Note一点不留情面,反复地践踏在Trumpets身上。

Most 32-bit C compilers share the convention used by 16-bit compilers, that the names of all global symbols they define are formed by prefixing an underscore to the name as it appears in the C program.

大多数32位的C编译器共享16位编译器的惯例约定,即它们定义的所有的全局标志符的名字在C程序中出现时由一个下划线加上名字组成。

N The time of preheating from 150°C to 200°C shall be within maximum 180 seconds

从150°C到200°C的预热时间应在180秒以内

The original work dates from c.615 BC, but it was updated and reedited c.550 BC.

原来的工作时间从c.615公元前,但它已更新和reedited c.550公元前。

The invention discloses an optical-fiber all-optical code converter, comprising a temperature control circuit as well as optical-fiber Mach-Zed interferometer, heat sink, semiconductor refrigerator, thermosensitive resistor and radiator, where the interferometer is composed of two cascaded 3dB couplers on the heat sink, the relation between length difference delta L of two arms of the interferometer and generated delay time difference delta T is that delta L=c.delta T/n, where c is light velocity in vacuum and n is refractive index of optical fiber; the thermosensitive resistor is used to detect temperature of interferometer, the temperature control circuit is used to compare feedback voltage of the thermosenstive resistor with a given voltage to regulate working current of the semiconductor refrigerator, and the semiconductor refrigerator refrigerates or heat two arms of the interferometer by the heat sink.

本发明公开了一种光纤型全光码型转换器,它包括温控电路和位于密封盒内的光纤型马赫-泽德干涉仪、导热热沉、半导体制冷器、热敏电阻以及散热片;干涉仪由位于导热热沉上的二个3dB耦合器级联而成,两臂的长度差ΔL与产生的延时差ΔT之间的关系为ΔL=c。ΔT/n,c为真空中光速,n为光纤折射率;热敏电阻用于对干涉仪的温度进行探测,温控电路用于比较热敏电阻反馈的探测电压和设定电压,对半导体制冷器的工作电流进行调节,半导体制冷器通过导热热沉对干涉仪两臂同时进行制冷或加热。

The problem of interlayer matrix rivetting in woven-reinforced composite materials was proposed.Moreover,the relationship between rivetting and GⅠC and GⅡC values was researched.

提出了在织物增强复合材料层合板中,基体在织物孔洞中形成层间铆接,并研究了其与层间GⅠC和GⅡC的关系。

A new skeletonizing method by combining c-LAG of horizontal and vertical direction is proposed. The main idea of the method is a knowledge based node validation and a rule for combining the skeletons from the two c-LAGs.

基本的思想是对水平和垂直c-LAG的基于知识的节点校验和一个组合水平和垂直c-LAG骨架的规则。

For wurtzite-type crystal, [Zn-S4]6- and [Zn-S4]6+ with different crystal positions distribute in the form of layers along c axis, where the two layers of tetrahedrons whirl 60 around c axis, so the crystal is mainly like hexagonal cone.

在纤锌矿型晶体中,[Zn-S_4]~(6-)和[Cd-S_4]~(6-)四面体沿c轴呈层状分布,上下两层四面体的结晶方位不同,两层四面体绕c轴旋转60°,晶体的结晶形貌为六方锥状。

The B, C, D group each mouse right armpit department hypodermic vaccinates the H22 lump cell to hang fluid 0.2ml, after vaccinating 24h weighing, and starts the abdominal cavity injection to give the medicine: A, B group uses physiological saline 0.2ml/only (continuously to give medicine 14 days) every day, C group with 5 fluorine uracil mg/day, dilutes the density is mg/0.2ml (the 1~5th day for medicine), D group will put down with the physiological saline disappears the hitch to compound mg/only (continuously to give medicine 14 days).

依据随机化原则,将40只ICR雌性小鼠随机分为四组,分别为空白对照组,荷瘤对照组,5氟尿嘧啶组,平消栓组。B,C,D组每只小鼠右腋部皮下接种H22瘤细胞悬液0.2ml,接种24h后称重,并开始腹腔注射给药:A,B组每日用生理盐水0.2ml/只(连续给药14天),C组用5氟尿嘧啶25mg/kg(第1~5天给药),D组用生理盐水将平消栓配制成1.0g/kg(连续给药14天)。

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Do you know, i need you to come back

你知道吗,我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。

Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。