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Methods:To produce antihuman DR5 monoclonal antibody(mDRA6) by immunizing BALB/c mice using DR5 protein. The change of DR5 expression after DDPtreated on HL60 cells was detected by FACS. Morphologic changes of HL60 cells were observed under fluorencence microscope. Cytotoxic and apoptotic effects of mDRA6 and DDP on HL60 cells were measured by MTT analysis. DNA fragmentation was detected by agarose gel electrophoresis.

DR5蛋白免疫BALB/c小鼠，融合筛选抗DR5杂交瘤细胞，制备抗DR5单抗——mDRA6；流式细胞术测定顺铂对HL60细胞表面DR5表达及细胞凋亡率；荧光显微镜下观察mDRA6与顺铂协同作用下HL60细胞形态变化；MTT法测定不同浓度的顺铂与mDRA6对HL60细胞存活的影响；琼脂糖凝胶电泳检测mDRA6与顺铂联合对HL60细胞DNA片段化的影响。

Methods: Mesenchymal stem cells were isolated from human term placenta by digestion of collagenase Ⅱ and their unique growth characteristic of attaching to the wall of cell culture flask. The proliferation ability was detected by living cell number counting and propidium iodide staining. Their surface markers were detected by flow cytometry. The cells were induced to osteoblast with dexamethasone, antiscorbutic acid and β-sodium glycerophosphate. And they also were induced to adipocytes with dexamethasone and insulin. After induction, the cells were observed by Von Kossa staining and oil red O staining.

将人足月胎盘组织经胶原酶Ⅱ消化和贴壁培养法获取间充质干细胞，运用活细胞计数和碘化丙吮检测其增殖能力；采用流式细胞术检测其细胞表面标志的表达；用地塞米松、抗坏血酸及β-磷酸甘油诱导其向成骨细胞分化，并用Von Kossa染色进行鉴定；用地塞米松与胰岛素诱导其向脂肪细胞分化，并以油红O染色进行鉴定。

The first thing to see, looking away over the water, was a kind of dull line -- that was the woods on t'other side; you couldn't make nothing else out; then a pale place in the sky; then more paleness spreading around; then the river softened up away off, and warn't black any more, but gray; you could see little dark spots drifting along ever so far away -- trading scows, and such things; and long black streaks -- rafts; sometimes you could hear a sweep screaking; or jumbled up voices, it was so still, and sounds come so far; and by and by you could see a streak on the water which you know by the look of the streak that there's a snag there in a swift current which breaks on it and makes that streak look that way; and you see the mist curl up off of the water, and the east reddens up, and the river, and you make out a log-cabin in the edge of the woods, away on the bank on t'other side of the river, being a woodyard, likely, and piled by them cheats so you can throw a dog through it anywheres; then the nice breeze springs up, and comes fanning you from over there, so cool and fresh and sweet to smell on account of the woods and the flowers; but sometimes not that way, because they've left dead fish laying around, gars and such, and they do get pretty rank; and next you've got the full day, and everything smiling in the sun, and the song-birds just going it!

往 水面上望去，首先看到的是灰蒙蒙的一条线——那是河对岸的树林子——别的便什么也看不清——接着是天空中有一点儿鱼肚白；然后鱼肚白多了些，逐渐朝四周散开去；接下来，远处河水的颜色淡了些，不是那么黑了，而是灰灰的了。更远处，可以看到小小的黑点子在漂过来——那是些载货的驳船之类。还有黑黑的一长条——那是木筏子。有时能听到长桨吱吱地响，或者一些杂音。四周这么寂静，声音又来自很远的远方。过了一会儿，你看到一道水纹。凭了水纹的模样，你知道那里有一块礁石，急流朝着它冲过去，流水飞溅，成了这个模样。你看到，雾气袅袅上升，离开水面，东方红了起来，河面红了起来。你可以看到对岸河边树林子边上一处原木搭成的小屋，那可能是一个木材场，在那里堆着的一堆堆木材，中间却是空的，容得狗钻来钻去，为了能叫人家上当①。然后微风轻拂，从河上一阵阵吹来，那么凉凉的，那么清新，闻起来那么甜美，这是全凭了那些树林子和那些鲜花的缘故。可有时候也并非全是如此美妙。

Water was claimed by Thales, Air by Anaximenes, Apeiron by Anaximander, Fire by Heraclitus.

水是人的泰利斯，阿那克西米尼空气， Apeiron的阿那克西曼德，消防的赫拉克利特。

Methods:All of 40 male Sprague-Dawley rats were divided into 3 groups:control group（group A, n = 10）, diabetic group for 4 weeks（group B, n = 14）, and diabetic group for 12 weeks（group C, n = 16）. The diabetes model was established by the injection of STZ. After some weeks erection function was examined by apomorphine injection. The content of NF-κB in the erectile tissue was evaluated by immunohistochemistry staining. At the same time the activity of NF-κB was detected by electrophoretic mobility shift assay.

将成年雄性SD大鼠40只分为3组：A组为正常对照组（10只），B组为糖尿病4周组（14只），C组为糖尿病12周组（16只），采用腹腔注射STZ法制作糖尿病模型。B组大鼠于4周后，A组、C组大鼠于12周后通过注射阿朴吗啡后评价勃起功能，并取阴茎海绵体组织免疫组化EnVision法分析NF-κB的分布与表达，同时采用电泳迁移率变动分析法进行NF-κB 活性检测。

Methods All of 40 male Sprague-Dawley rats were divided into 3 groups: control group (group A, n=10), diabetic group for 4 weeks (group B, n=14), and diabetic group for 12 weeks (group C, n=16). The diabetes model was established by the injection of STZ. After some weeks erection function was examined by apomorphine injection. The content of NF-κB in the erectile tissue was evaluated by immunohistochemistry staining. At the same time the activity of NE-κB was detected by electrophoretic mobility shift assay.

将成年雄性SD大鼠40只分为3组：A组为正常对照组（10只），B组为糖尿病4周组（14只），C组为糖尿病12周组（16只），采用腹腔注射STZ法制作糖尿病模型。B组大鼠于4周后，A组、C组大鼠于12周后通过注射阿朴吗啡后评价勃起功能，并取阴茎海绵体组织免疫组化EnVision法分析NF-κB的分布与表达，同时采用电泳迁移率变动分析法进行NF-κB活性检测。

Results: among the 112 adr cases,78.57% were reported by nurses and 19.64% were reported by doctors,91.96%(103 cases) were induced by intravenous injection; 58.93%(66 cases) were associated with antibiotics and followed by chinese drug preparations, 47.32%(53 cases) were manifested as lesions of skin and the appendant.

结果：报告人以护士和医师居多，分别占78.57%、19.68%。静脉途径给药的adr 103例，占91.96%，是引起adr的主要途径。导致adr的药品以抗菌药物最多，共66例，占58.93%。临床表现主要以皮肤及附件损害为主，共53例，占47.32%。

New bone formation was observed to be increasing significamly in both DCN bone cements and DC bone cements with the passage of time. By the end of 24 weeks, new bone had bridged the gap between the proximal and distal fragments in DCN bone cements and DC bone cements. By histomorphological detection in DCN bone cements, Abundant of chondral and new bone islands were seen at the site of defects 4 weeks after the operation and a number of new bones formed and enlarged by both enchondral ossification and appositional formation by the 8th weeks.

研究发现，DCN骨水泥植入4周大量间充质细胞分化，在材料与骨端之间出现一层软骨细胞，软骨细胞继续增生并向编织骨分化；8周材料降解的同时，小梁骨出现；12周新骨长入材料并已经基本替代完成，形成初步骨性连接；24周骨缺损完成骨性连接，新骨进行结构改建，材料内部形态结构明显变化。

And E.N.E: Had they seen the Island, as I must necessarily suppose they did not, they must, as I thought, have endeavour'd to have sav'd themselves on Shore by the Help of their Boat; but their firing of Guns for Help, especially when they saw, as I imagin'd, my Fire, fill'd me with many Thoughts: First, I imagin'd that upon seeing my Light, they might have put themselves into their Boat, and have endeavour'd to make the Shore; but that the Sea going very high, they might have been cast away; other Times I imagin'd, that they might have lost their Boat before, as might be the Case many Ways; as particularly by the Breaking of the Sea upon their Ship, which many Times obliges Men to stave, or take in Pieces their Boat; and sometimes to throw it over-board with their own Hands: Other Times I imagin'd, they had some other Ship, or Ships in Company, who upon the Signals of Distress they had made, had taken them up, and carry'd them off: Other whiles I fancy'd, they were all gone off to Sea in their Boat, and being hurry'd away by the Current that I had been formerly in, were carry'd out into the great Ocean, where there was nothing but Misery and Perishing; and that perhaps they might by this Time think of starving, and of being in a Condition to eat one another.

如果他们发现这个小岛，我想他们一定会用船上的救生艇竭尽全力划到岸上来的。但看来他们一定没有看到小岛，只是鸣枪求救，尤其是他们看到我燃起的火光后，更是多次放枪。由此我头脑里出现了种种设想。首先，我想到，他们看到我点燃的火光后，必然会下到救生艇里拼命向岸上划来，但由于风急浪高，把他们刮走了。一会儿我又猜想，也许他们的救生艇早就没了，这种情况是经常发生的。当大船遇到惊涛骇浪时，水手们往往不得不把船上的救生艇拆散，甚至干脆扔到海里去。过会儿我又想，也许与他们结伴同行的船只，在见到他们出事的信号后，已把他们救起来带走了。我又想到，说不定他们已经坐上救生艇，可是遇到了我上次自己碰上的那股急流，给冲到大洋里去了。到了大洋里，他们可就糟了，那是必死无疑的。说不定这会儿他们都快饿死了，甚至可能正在人吃人呢！

In the Expo of 1888, Gaudi was charged by the Marquis of Comillas the design of the Pavilion of the Transatlantic Company in the Maritime section. That same year, Gaudi was flooded with a wide variety of projects. Of these the Episcopal Palace of Astorga was the only one censored by the Royal Academy of the Bellas Artes of San Fernando. Because it was a public work charged by the then Ministry of Justice and Grace, that his plans were so minutely refined that the project began in 1893, two years after it had been proposed. By this time, the Bishop had passed away and Gaudi had antagonized the clergy, leaving the project.

在1888年的万国博览会，高第受Comillas侯爵之邀，负责设计大西洋彼岸的海运部门的摊位棚帐，之后在同一年，高第受到大量的计画邀约，而其中一件Astorga主教广场的计画被San Fernando艺术皇家学院审核不过关，因为这是一件由教会的正义、恩典部门教士们所授与的公共建筑计画，所以计画不断地、精密地被审核，直到1893年---计画提出的第二年才开始动工，此时主教已过世，高第也因此对这些神职人员不满而放弃了这个计画。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。