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Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%,0 and 38% respectively in sorted GPA positive cells of 3 samples by FISH,and the results of GPA+ cells of the 3 cases by PCR were positive,negative and positive respectively,which were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16～24周的孕妇血中分离出NRBCs，并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果，3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%；其PCR结果，分别为阳性、阴性、阳性，并在引产后得到性别证实。

Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%0 and 38% respectively in sorted GPA positive cells of 3 samples by FISHand the results of GPA+ cells of the 3 cases by PCR were positivenegative and positive respectivelywhich were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16～24周的孕妇血中分离出NRBCs，并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果，3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%；其PCR结果，分别为阳性、阴性、阳性，并在引产后得到性别证明。

Abortus, amplify BP26 gene by PCR and clone to pMD18-T simple vector. Identify the constructed recombinant plasmid pMDBP26 by sequencing, then subclone to vector pET-28a. Transform the constructed recombinant plasmid pETBP26 to E. coli BL21(DE3) for expression under induction of IPTG. Purify the expressed product by histidine-binding resin column chromatography and identify by Western blot.

提取布鲁氏菌基因组DNA，用PCR扩增出BP26基因，并克隆到pMD18-T载体上，测序正确后，再亚克隆至pET-28a载体上，转化到大肠杆菌BL21(DE3)中，用IPTG诱导表达，经组氨酸结合树脂柱纯化，并对纯化后的表达产物进行Western blot鉴定。

The plasma density scale length decided by main laser pulse in the experiment is suitable for Vacuum Heating, and it is the domiant process for hot electron generation. From the scaling law about hot electron temperature given by VH model, the hot electron measured by experiment was reasonable, the temperature was higher than the temperature given by Rcsonant Absorption scaling law.

从真空吸收给出的超热电子温度的定标率来看，实验中测量得到的超热电子温度是合理的。X射线是各向同性的，X射线产额随入射激光强度的降低而减小，当强度小于10〓W/cm〓时，不再有硬X射线产生。

Aqueous extract of cotton has allelopathic effect on Raphanus sativus, Brassica campestris, Lactuca indica, Brassica campestris, Echinochloa crusgall, Setaria viridis and Abutilon theophrasti and its inhibition effect gradually strengthens with increasing concentration. Aqueous extracts from stems, leaves and roots of cotton inhibit or accelerate seed germination and seedling growth, and activity of enzyme of seedling root of cotton. While aqueous extracts from stems and leaves of cotton are 0.02 gmL^(-1), root length, shoot length, lateral root number and dry weight of cotton respectively increase by 10.3%, 3.1%, 22.9% and 2.4% over the control. The above mentioned indexes are lower than the control by 34.4%, 39.2%, 40.6% and 19.0% respectively as the aqueous extracts are 0.1 gmL^(-1). At the same time, root activity and SOD activity decrease by 38.2% and 36.7%, while POD activity and MDA content increase by 52.3% and 36.5%. Growth indexes of plant receptors increase at low concentrations but decrease at high concentrations of aqueous extracts. The allelopathic effect of aqueous extract of stems and leaves is stronger than that of root.

结果表明：棉花植株水浸提液对萝卜、白菜、莴苣、油菜、稗草、狗尾草、苘麻均具有化感效应，随着作用浓度增加，抑制效应逐渐加强；棉花茎叶和根系水浸提液对棉花种子萌发、幼苗生长、幼苗根系活力、根系酶活性有抑制或促进作用，当茎叶浸提液浓度为0.02 gmL^(-1)时，棉花根长、苗高、侧根数、干重分别比对照增加10.3%、3.1%、22.9%和2.4%，当浓度为0.1 gmL^(-1)时，上述指标与对照相比分别降低34.4%、39.2%、40.6%和19.0%，而根系活力、SOD活性比对照降低38.2%、36.7%，POD活性、MDA含量较对照增加52.3%和36.5%，不同浓度棉花植株水浸提液对棉花生长呈现"低促高抑"的规律，且茎叶浸提液作用效应大于根系浸提液。

By synthesizing theories of systems engineering, science of talent, talent assessment, performance appraisal and bibliometrics, the author analyzes the qualification structure and performance structure of theachers in military academies and sets up a basic structure and concrete appraisal indicator system of qualifications of technical specialty for teachers in colleges. The author puts forward new appraisal indicators such as "lecuring hours coefficient","republication coefficient","thesis of core journals coefficient" and "citation coefficient". Comparatively complete quantification approaches are invented by the author for working performance of teaching, researching and social service of teachers. Based on this, the author establishes an integrated method to generate the appraisal results. By the application of stadardized processing on the appraisal results and synthetization of weighted indexes, the author solves the addibility and comparability problems of differentiated appraisal results by category or dimensiok, and realizes the amalgamation and comprehensive appraisal of inflexible appraisalresults and flexible appraisal results, and probes into the testification methods of validity, consistency and sensitivity analysis for the indicators system.

综合运用系统工程理论、人才学理论、人才素质测评理论、工作绩效评估理论、文献计量学等原理与方法，分析研究了军校教师的素质结构和绩效结构；建立了高校教师专业技术资格评审条件基本结构和具体评价指标体系，提出了"完成主讲课程课时系数"、"著作重版系数"、"核心期刊论文系数"、"论文被检索引用系数"等若干新的评价指标；提出了比较完整的教师的教学、科研、社会服务等工作业绩的量化方法；以此为基础，建立了评价结果的综合方法，通过对评价结果的标准化处理，运用指标加权综合法，解决了不同类别、不同量纲指标的评价结果的可加性和可比较性问题，实现了硬评价结果和软评价结果的融合和综合评价；探讨了指标体系有效性、一致性、灵敏度分析的检验方法。

The author has limited the topic to only five of the new approaches:1 the reaction to the Romantic criticism by the historical critics, 2the attacks on the "God of Idolatry" by the unbradleyan critics, 3the revelation of Shakespeare s temperament by linguasymbolic critics, 4the study of Shakespeare s motivation for his dramatic creation by the psychoanalytic critics, and 5the selfportrait of Shakespeare by Rowse.

他们以纯粹审美的观点来研究剧作中人物的思想感情和一言一行，企图从中发现莎翁深刻的、首尾连贯的戏剧意图，并且根据人物的内心规律来解释人物的一切表现。这些有着敏锐批评洞察力的浪漫派莎评家们依靠自己的艺术直觉把握莎士比亚，揭示了莎士比亚在戏剧手法和人物刻划方面的惊人天赋；但这种莎士比亚评论最后发展成了莎士比亚崇拜。

But as my mother often said, ''They asked for trouble'' —by the way they make their arguments, by their puzzlingly shoddy scholarship, by what they emphasize and de-emphasize, by what they leave out and by writing on this sensitive topic without doing extensive interviews with the lobbyists and the lobbied.

他们制造的这些争议，他们偷换的概念，他们强调的和忽略的，他们轻率的未经调查访问就提出的那些关于游说组织的敏感论调，所有这些就像我妈妈说的那样，&他们在自找麻烦&。

When 〓 is the family of bipartite graphs,the 〓 capacityand the 〓 valency of a graph G are referred to as the biparticitydenoted by β(Gand the bivalencydenoted by 〓(Gof G respectively;when 〓 is the family ofgraphs whose components are complete bipartite graphs,the 〓 capacity and the〓 valency of a graph G are referred to as the complete biparticitydenoted byc(Gand the complete bipartite valencydenoted by 〓(Gof G respectively;andwhen 〓 is the family of star forests,the 〓 capacity and the 〓 valency of a graphG are referred to as the star arboricitydenoted by st(Gand the star valency ofG(denoted by 〓)respectively.

以v记图G的完全二部分解中顶点υ所关联的π中完全二部图的个数，记〓这里π取遍G的所有完全二部分解。我们称υ为G的完全二部次。对一般的图，当图的阶数充分大时，P。〓和L.Pyber给出了υ的一个上界，即，〓。F.R.K.Chung等人及Z.Tuza的结果说明这一上界是不可改进的。对υ的具体值，即使是对某些特殊的图类，结果也知之甚少。P.C.Fishburn和P.L.Hammer给出了一个相关的结果：当n≤16时，〓，（在υ的定义中，以完全二部覆盖替代完全二部分解，即得〓。同时他们提出了下述公开问题。问题1。〓=？

Matters have gone far enough, with the Pingkiang Massacre in Hunan, the Chuehshan Massacre in Honan, the attack on the Eighth Route Army by Chang Yin-wu in Hopei, the attack on the guerrillas by Chin Chi-jung in Shantung, the ruthless slaughter of between five and six hundred Communists by Cheng Ju-huai in eastern Hupeh, the large-scale assaults on the garrison forces of the Eighth Route Army by the Central Army in eastern Kansu and, more recently, the tragedy in Shansi where the old army attacked the new army and invaded positions held by the Eighth Route Army.

演变所极，湖南则有平江惨案，河南则有确山惨案，河北则有张荫梧进攻八路军，山东则有秦启荣消灭游击队，鄂东有程汝怀惨杀共产党员五六百之众，陇东有中央军大举进攻八路驻防军之举，而最近山西境内复演出旧军攻击新军并连带侵犯八路军阵地之惨剧。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。