查询词典 by-your-leave

1.To study the expression of in the different glucose concentration with the time changing. 2.To study the effect of different concentration of Hepatocyte Growth Factor on the Integrin-linked kinase in protein and mRNA level by Mesangial Cells in high concentration (25 mmol/L) of glucose. 3.To study the effect of different concentration of HGF on the Fibronectin'secretary by Mesangial Cells. 4.To study latent therapeutic action and possible mechanism of HGF on the glomeruler sclerosis. The difference of ILK's experession on mRNA level in different concentration of glucose and HGF are detected by means of revers transcription-polymerase chain reaction,and by means of immunohistochemistry to detect their difference in protein level;the effection of HGF to the FN's secretary in high glucose are detected by means of enzyme linked innunosorbent assay.

1探讨在不同的血糖浓度下大鼠肾小球系膜细胞中ILK伴随时间变化在蛋白和mRNA水平表达的差异 2探讨不同浓度的HGF对高糖环境下(25mmol/L)大鼠肾小球系膜细胞中ILK在蛋白和mRNA水平表达的影响 3探讨不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响 4初步探讨HGF对肾小球硬化的潜在的治疗作用及其可能机制采用RT-PCR的方法分析在不同血糖浓度和不同HGF浓度下大鼠肾小球系膜细胞中ILK在mRNA水平表达的差异；采用免疫组织化学的方法直观的观察它们在蛋白水平表达的差异；采用ELISA法分析不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响。

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry，FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时，对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后，Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR，RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时，Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope，LSM)观察北豆根总碱(0、40μg/ml)作用24小时后，Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

David Copperfield by Dickens, Rouge et Noir by Stendhal, History Study by Toynbee, Sea Wolf by Jack London, the philosophy of existentialism, and the novels by Merimee, etc. have affected the life of Wang Shi quite a deal.

狄更斯的《大卫·科波菲尔》，司汤达的《红与黑》，汤因比的《历史研究》，杰克·伦敦的《海狼》，存在主义哲学和梅里美的小说等都对王石的生活产生过重要影响。

METHODS: RAW264.7 macrophages growth inhibition was measured by MTT assay. The apoptosis effect of RAW264.7 murine macrophages induced by ox-LDL was analyzed by flow cytometric analysis and DNA agarose electrophoresis. After expression of Daxx was silenced by special siRNA, the macrophages apoptosis was observed by AO/EB fluorescence staining. Oil Red O Dyeing experiment was used to show the cellular lipid droplets in lipid-loaded cells. High performance liquid chromatography analysis was performed to determine the content of cellular cholesterol. Real time RT-PCR was used to detect the mRNA expressions of Daxx and SCAP.

用不同浓度的氧化型低密度脂蛋白处理RAW264.7细胞48h，MTT法检测ox-LDL对RAW264.7细胞生长的影响；流式细胞术和DNA断裂片段分析法研究ox-LDL诱导的细胞凋亡；用特异性siRNA沉默Daxx在RAW264.7细胞中的表达，通过AO／EB染色观察基因沉默后的细胞凋亡形态学改变；高效液相色谱检测细胞内胆固醇含量；油红O染色观察细胞内脂滴的形成情况；Real time RT-PCR检测细胞内Daxx mRNA、SCAP mRNA的表达情况；用Western Blot印迹法检测caveolin-1蛋白的表达。

Resolved, therefore, That, being invested by the Creator with the same capabilities, and the same consciousness of responsibility for their exercise, it is demonstrably the right and duty of woman, equally with man, to promote every righteous cause, by every righteous means; and especially in regard to the great subjects of morals and religion, it is self-evidently her right to participate with her brother in teaching them, both in private and in public, by writing and by speaking, by any instrumentalities proper to be used, and in any assemblies proper to be held; and this being a self-evident truth, growing out of the divinely implanted principles of human nature, any custom or authority adverse to it, whether modern or wearing the hoary sanction of antiquity, is to be regarded as self-evident falsehood, and at war with the interests of mankind.

因此，决议认为，由于造物主赋予男女相同的能力和运用这些能力的相同的责任感，所以男女显然都有相同的权利和责任，利用一切正义的手段促进一切正义的事业；尤其在道德和宗教这些重大的问题上，不言而喻，妇女有权同她的兄弟一道，在私下和公开的场合，通过文章和演讲，运用任何合适的手段、在任何合适的集会上，开宗名义地宣讲。这是不言自明的真理，它是从扎根在人类本性的神圣原则中产生的，任何与之相违背的习俗和权威，不论是现代的还是被磨损得苍白的古老法则，都将被视作不言而喻的谬误，是违背人类利益的。

It mainly covers following items: administrative and institutional fees, funds and extra charges that are stipulated by laws and regulations; administrative and institutional fees approved by the State Council and provincial government and their financial and planning departments; funds and extra charges established by the State Council and the Ministry of Finance; funds turned over to competent departments by their subordinate institutions; self-raised and collected funds by township governments for their own expenditure; and other financial funds that are not covered in budgetary management.

其范围主要包括：法律、法规规定的行政事业性收费、基金和附加收入等；国务院或省级人民政府及其财政、计划部门审批的行政事业性收费；国务院及财政部审批建立的基金、附加收入等；主管部门所属单位集中上缴资金；用于乡镇政府开支的乡自筹及乡统筹资金；其他未纳入预算管理的财政性资金。

The principal purpose of this study is to set up the isolation methods for diphtheria toxin expressed by genes, to purify the toxin with complete biological activities, and to make researches on its toxicities. In the experiments, the target gene was inserted into the cloning vectors--pET22b, then the vectors were introduced into host cells -BL21(DE3). The expression of the toxin was induced by addition of IPTG. The expression products were purified by His-6 label affinity chromatography. Then the diphtheria toxin expressed was transferred to PVC membrane using semidry blotter to determine the amino acid sequence of N-terminal. The "nicked" diphtheria toxin linked by disulfide bond was gained by protease hydrolysis.

为建立重组基因表达白喉毒素的分离纯化方法，制备出具有生物活性的蛋白质毒素，构建了白喉毒素表达载体pET22b-DT，并转入大肠杆菌BL21(DE3)中诱导表达，以融合表达的His-6作为标签通过亲和层析纯化；表达毒素经蛋白酶酶切后，形成了以二硫键连接的与天然结构相同的带"缺口"的白喉毒素，并测定白喉毒素对豚鼠的急性毒性和细胞毒性。

The seeds of discontent were planted by Adolph Berle and Gardiner Means (1932) in relation to the separation of ownership from control, by Ronald Coase (1937) with reference to the puzzle of vertical integration and the theory of the firm, by Chester Barnard (1938), who featured adaptation of a cooperative kind accomplished through hierarchy (as opposed to adaptation through the market in response to changes in relative prices), by the 'marginalist controversy' of the 1940s, and by Armen Alchian's (1950) appeal to evolutionary arguments to explain observed economic regularities.

不满的种子由此而种下，例如， Adolph Berle 和 Gardiner Means （1932）有关所有权和控制权分离的研究， Ronald Coase （1937）对纵向一体化难题和企业理论的关注， Chester Barnard （1938）对一种通过层级实现的合作式调节方式（相对于通过市场响应相关价格改变而实现的调节方式）的强调，20世纪40年代的"边际主义论战"，以及 Armen Alchian （1950）对用演进观点来解释观察到的经济规律的呼吁。

The main representations for agriculture industrialization to improve our agriculture economy are: first, to solve the contradiction between the small production of our agriculture and big international market; second, to heighten the comparative efficiency of agriculture by specialization of production; third, to promote the process of urbanization by widening the traditional industrial chain. Fourth, to solve the contradiction between specializationproduction and social serves by making foreign trade systems of agriculture goods sound. fifth, to raise diathesis of farmers by spreading agriculture techniques; sixth, to promote standardization and internalization of agriculture by making the standardization system.

农业产业化经营促进我国农业经济发展的主要表现是：第一，解决了我国农业小生产和国际大市场之间的矛盾；第二，实现了专业化生产，提高了农业的比较效益；第三，拓展了传统的产业链，促进了农村城镇化进程；第四，健全了农产品外贸流通体制，解决了专业化生产和社会化服务之间的矛盾；第五，促进了农业科技的推广，提高了农民的素质；第六，健全了农业标准体系的建设，促使农业走向规范化、标准化、国际化。

The stable and reliable operation voltage of the probe can be given by the following methods:(1) 20% light coupling efficiency is obtained by direct coupling of LD-optical fiber.(2) The light power is transmitted by multi-model optical fiber.(3) The largest photo-electric conversion efficiency is achieved by selecting the transformer regulation of step-up transformer.(4) The stable light intensity is achieved by using LD light feedback of driving circuit and refrigeration.

通过下述方法可提供稳定、可靠的探头工作电压：LD—光纤的直接耦合方式，可实现20％左右的光耦合效率；采用多模光纤传输功率；选择升压变压器最佳变比提高光电转换效率；采取LD驱动电路光反馈以及致冷措施使其发光幅值稳定。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。