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Results: six patients with vagal reflection were cured by intravenous atropine, 2 with pericardial hemorrhage were treated by pericardiocentesis, 1 patient developed hypotension due to occlusion of the mitral valve orifice by the catheter was corrected by shortening the duration of the catheter in left ventricle, the blood pressure of 1 patient with rapid atrial flutter was elevated by intravenous cedilanid, the blood pressure of 1 patient with hypotension of unknown origin was elevated by vasopressor and atropine.

结果：其中迷走神经反射6例，经静脉注射阿托品纠正；心包出血2例，心包穿刺抽液血压至正常；球囊导管阻塞二尖瓣口1例，缩短导管停留在左心室时间，可以避免低血压反应；快速心房颤动1例，使用西地兰减慢心室率，血压回升；原因不明1例，经使用升压药及阿托品后血压改善。

2 Any notice required or permitted hereunder shall be in writing and shall be deemed given as of the date it is: delivered by hand; by overnight or next day delivery; received by Registered or Certified Mail, postage prepaid, return receipt requested; or received by facsimile, as can be presumptively demonstrated by return fax or letter demonstrating successful facsimile transmission; and addressed to the party to receive such notice at the address and/or facsimile telephone number set forth below, or such other address as is subsequently specified to the notifying party by the receiving party in writing.

11.2 本协议项下所有通知应当以书面形式为之，并且在下列情况下视为已经交付：专人递送；经由隔日送达业务递送；由挂号邮件或保证邮件方式接受，邮资预付，需要回执；由传真接收，并回传真或信函证明已经成功发送；通知应当按照下列地址送交当事人，或按照下列电话号码传送，或者按照接受一方最近书面通知的地址或号码为准

Personality traits were evaluated by Eysency Personality Questionnaire that was compiled by Eysency and revised by Gong. Standard scores of Psychoticism, Extraversion and Neuroticism were calculated, and taking 50 points as a boundary. Each dimension was assigned into 2 grades non-psychotic type (P0) psychotic type (P≥50), introversion type (E0), extraversion type (E0), non-nervous type (N0) and nervous type (N≥50). 5 mL ulnar vein blood was obtained without adding decoagulant, and then serum was removed. Genome DNA was extracted from blood clot by conventional chloroform for PCR analysis. PCR product was sequenced by pyrophosphoric acid. SNP was determined by PSQ96 MA sequenator. Allele frequency was analyzed by mass spectrometry.

人格评定采用Eysency编制，龚耀先修订的艾森克人格问卷、计算精神质，内外问，神经质3个个性维度的标准分，并以50分为界，将各维度分为两个等级：非精神病倾向型（精神质0）、精神病倾向型（精神质≥50）；内倾型（内外向0），外倾型（内外向≥多50）；非常经质倾向型（神经质0），神经质倾向型（神经质≥50）取肘静脉血5 mL，不加抗凝剂，弃血清，血凝块常规酚氯仿提取基因组DNA，进行PCR分析PCR产物应用焦磷酸测序PSQ96 MA测序仪进行SNP检测，用质谱法进行及等位方基因频率分析。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

The method according to claim 6, for manufacturing a blade wherein a part external to the shell comprises a blade rooting part having at least one attachment bar, in which an end portion for linkage to a hub of said rotor is fitted with a laminate attachment, also comprising thermoplastic composite, and extending said at least one spar bar housed in the shell, the method further comprising the step of producing the elementary piece comprising the at least one spar by producing each attachment bar and the spar bar in the form of a strip of continuous and unidirectional reinforcing fibres which are agglomerated by said thermoplastic matrix, obtained by pultrusion at a melting temperature of the matrix, of several plies of continuous and unidirectional fibres which are agglomerated by said matrix, and, if said rooting part comprises at least two attachment bars, each one of which respectively extends one spar bar, in superimposing parts of corresponding strips forming the spar part housed in the shell, with interposition of at least one ply of continuous and unidirectional fibres which are agglomerated by said matrix between the adjacent superimposed strip parts, and fitting a removable tool, in the shape of a wedge, between parts held spaced from each other of two neighbouring strips, to form two attachment bars of the rooting part, then arranging the strips in a mould, and inserting each linkage end portion of a pultruded strip between two laminate elements of the attachment, to superimpose all the laminate elements of the attachment, closing the mould and compacting its contents under pressure and at a melting temperature of said thermoplastic matrix, and solidifying the matrix by cooling to rigidify the spar thus obtained with the laminate attachment solidly attached to its rooting part.

该方法根据索赔六，制造业刀片其中的一部分，罐壳外部包括一个叶片生根的一部分，拥有至少一个实习酒吧，在其中一结束部分为联系枢纽的说，转子是带有附件的层压板，也包括热塑性复合材料，并延长说，至少有一石酒吧住在蚬壳公司，进一步组成的方法步骤，生产初级一块组成的至少有一个SPAR公司生产每个附件的酒吧和酒吧在SPAR公司的形式，带不断加强和单向纤维是由烧结说，热塑性矩阵，得到的拉挤在熔化温度矩阵，数层的连续和单向纤维是由烧结说，矩阵，如果说，生根部分组成，至少有两个实习酒吧，每个人都分别延长一石酒吧，在叠加部分，相应的带形成的SPAR公司的一部分，居住在蚬壳公司，与干预的至少有一单层连续和单向纤维是由烧结说，矩阵之间的毗邻叠加条形零件，及装修一个可移动的工具，在形状，离间，部分之间举行的间隔从对方的两个邻国带，形成两个附件酒吧的生根部分，然后安排带在一个模具，并插入每个联系年底部分一拉挤带之间的二板的要素扣押，叠加所有层压板的要素扣押，关闭模具和压缩其内容的压力下和在熔化温度说，热塑性矩阵，并巩固矩阵冷却 rigidify该SPAR公司，从而获得与层压板的附着牢固重视其生根的一部分。

All those whom God has predestinated to life, and those only, he is pleased, in his appointed and accepted time, effectually to call, Ro 8:30 11:7 Eph 1:10,11 by his word and Spirit, 2Th 2:13,14 2Co 3:3,6 out of that state of sin and death in which they are by nature, to grace and salvation by Jesus Christ; Ro 8:2 Eph 2:1-5 2Ti 1:9,10 enlightening their minds spiritually and savingly to understand the things of God; Ac 26:18 1Co 2:10,12 Eph 1:17,18 taking away their heart of stone, and giving to them an heart of flesh; Eze 36:26 renewing their wills, and by his almighty power determining them to that which is good; Eze 11:19 Php 2:13 De 30:6 Eze 36:27 and effectually drawing them to Jesus Christ; Eph 1:19 Joh 6:44,45 yet so as they come most freely, being made willing by his grace. So 1:4 Ps 110:3 Joh 6:37 Ro 6:16-18 2 This effectual call is of God's free and special grace alone, not from any thing at all foreseen in man; 2Ti 1:9 Tit 3:4,5 Eph 2:4,5,8,9 Ro 9:11 who is altogether passive in it, until, being made alive and renewed by the Holy Spirit, 1Co 2:14 Ro 8:7 Eph 2:5 he is enabled by it to answer this call, and to embrace the grace offered and conveyed in it.

2Jo 1：9-11 1Co 16：22 Ga 1：6-8 1 ）所有的人对上帝有predestinated人生观，以及那些只，他很高兴，在他的任命，并接受了时间，有效地打电话，滚装8：30 11时07分弗1：10，11由他的文字和精神， 2th 2：13，14 2co 3：3，6出该国的罪恶和死亡的来信，他们都是本质上说，以恩典和救赎，由耶稣基督；滚装8：2弗2：1-5 2ti 1：9，10启发他们的头脑，在精神上和savingly了解事情的上帝；交流电26：18 1co 2：10，12弗1：17，18拿走他们铁石心肠，并给他们一个心脏的果肉；新教徒协会36：26自强不息他们的遗嘱，以自己的全能的能力决定他们说，这是个好；新教徒协会11时19分的PHP 2时13德30：6新教徒协会36：27 ，并有效地借鉴他们耶稣基督；弗1：19 joh 6：44，45然而，让他们来最自由，正在取得愿意由他的恩典，所以1：4的PS 110：3 joh 6时37滚装6：16-18 2 ）本显效呼吁是上帝的自由和特别恩典单，而不是由任何事情在所有能预见在男子； 2ti 1时09山雀3：4，5厄2：4，5，8，9滚装9时11分，他们是完全处于被动，直到，正活着，并再次由圣灵1co 2时14滚装8时07弗2时05分，他是启用的，它要回答这个号召，并接受恩典提供，并转达了此事。

The experimental results indicated that the addition of yellow serofluid had better nitrogen source effects than carbamide with its optimal addition level as 2 %(cell concentration increased by 18 %, bud ratio increased by 16 %, and liquor yield by alcohol fermentation increased by 2.2 %), which could settle the problem of environment pollution induced by direct discharge. The use of yellow serofluid in the production of alcohol from cassava could increase alcohol content in fermenting solution and liquor yield by 14.2 %Vol and by 35.5 % respectively.

结果表明，添加黄浆水作为酒母培养的氮源效果优于尿素；最适添加量为2 %；成熟酒母发酵液中细胞浓度比尿素作营养盐时提高18 %，出芽率提高16 %，酒精发酵出酒率提高2.2 %；可解决直接排放带来的环境污染问题；用于木薯原料酒精发酵，发酵液中酒精度可达14.2 %Vol，原料出酒率为35.5 %。

The mian works are as follows:(1)By coprecipitation technique and coprecipitation- gel technique the nano zirconia powder was produced, factors affecting the properties of nano zirconia powder such as pH and the content of stabilizator are analyzed;(2)By the new pressless sintering process, the nano zirconia ceramic was produced, factors which can affecting the properties of nano zirconia sinter body such as moulding press and the sinter temperature are analyzed;(3)By the microtherm pressless sintering process, the nano copper added functional zirconia ceramic composite was produced, microscopic dimensionality and electric properties of such sinter body and the factors affecting such sinter body are analyzed;(4)By the self-invented "external albumen coating" technique the zirconia powder was successfully coated by carbon in the corresponding sinter body, which can help solve the traditional aggregation problem to some degree;(5)Based on analyze the characteristics in the nano powder sinter process, the "three-ball sinter model" was proposed, for the relationship between the relative density and porosity of sinter body, by mathematical fitting, such model was successfully verified.

本研究比较系统地研究了纳米氧化锆陶瓷的合成与成型机理，特别是讨论了纳米氧化锆陶瓷的稳定性，为防止纳米颗粒在外场作用下的团聚和长大提供了理论基础和实验数据，具体工作有以下几方面：(1)采用共沉淀法和共沉淀-凝胶法制备了纳米氧化锆粉体，并分析了pH值和稳定剂含量对粉体性能的影响；(2)采用新型无压烧结工艺制备了纳米氧化锆陶瓷，并分析了成型压力和烧结温度对烧结体性能的影响；(3)采用低温无压烧结工艺制备出添加纳米铜的功能性纳米氧化锆复合陶瓷，并分析了烧结体的微观尺度和电学性能的变化以及影响因素；(4)通过自创的&鸡蛋清外敷法&在真空镀膜台上对纳米氧化锆粉体进行包敷碳颗粒的表面处理，并采用真空烧结工艺制得相应烧结体，初步开始解决陶瓷烧结过程中团聚长大这一传统难题；(5)通过分析纳米颗粒在制备烧结体过程中的实际特点，提出了&三球烧结数学模型&，通过引入坯体相对密度和孔隙率的相对关系分析并通过自行编写的数学拟合软件，验证了这一数学模型。

Water samples were extracted and purified and collected by solid-phase extraction LC-18 column. Soil samples were extracted by ethyl acetate/petroleum ether (2:1, by vol) and purified by sulphonation. Wheat sample was extracted by acetonitrile/water (4:1, by vol) and purified and collected by SPE- Florisil column.

水样直接用LC-18固相萃取小柱分离、净化和富集；采用乙酸乙酯／石油醚（体积比2：1）振荡提取，磺化法净化，测定土壤中氯氟吡氧乙酸残留量；以乙腈／水（体积比4：1）为提取剂，氟罗里硅土固相萃取小柱分离、净化，测定小麦样品中氯氟吡氧乙酸的残留量。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。