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In this thesis,the AM1,MNDO,MINDO/3(mainly AM1)and INDO/S-CI semiempirical MO methods were used toinvestigate the excited-state intramolecular protontransfer reactions of salicylic acid derivatives—salicylic acid,methyl salicylate,salicylaldehyde,o-hydroxyaceto-phenone,salicylamide and 3-hydroxy-picolinamide (6 conformers and 2-3 anion species);2-(2'-hydroxy-5' methylphenyl) benzotriazole(4 conformers),2-(2' hydroxyphenyl) benzimidazole (3 conformers and 3anion species),Bis-2,5-(2-benzoxazolyl)hydroquinone(3 conformers),2-(2'-hydroxyphenyl)benzothiazole(2conformers) and 7-azaindole dimer (2 conformers).Theinvestigations were described as follows.Geometry optimization,relative stability andhydrogen bonding energy First,for sylicylic acid derivative molecules,the AM1,MNDO and MINDO/3 methods were used toinvestigate ground-state geometry optimization,energies,relative stabilities and hydrogen-bondingenergies on the five kinds of the molecules(designing 6 conformers and 2-3 anion species).Comparing with experimental data,the optimizedgeometry,the order of stability,the hydrogen-bonding energies and the distances between O-O in O-H..O hydrogen bonds by AM1 method were in agreementwith the experimental data,however,the C-C bondlengths optimized by MNDO and MINDO/3 were longer,C-O and O-H bond lengths were shorter;for C-N bondlengths,the results opitimized by MNDO method werethe same as those by AM1 method,nevertheless the C-Nbond lengths given by MINDO/3 method were muchshorter.For some sylicylic acid derivatives(e.g.methyl salicylate,salicylamide),the order ofstabilities on the conformers given by MNDO andMINDO/3 methods were not in agreement with theexisting conformers deduced by experimental methods,and the hydrogen bonding energies calculated by MNDO.and MINDO/3 methods were smaller.Second,the studyon the other systems found that the optimizedgeometry of the proton-transfered product with INDOmethod could not be obtained,only could theoptimized geometry of reactant be obtained,and thecalculated hydrogen bonding energies were greater.Many results of calculation indicated that the studyon the excited-state intramolecular proton transferreaction system using AM1 method was suitable andreliable.

本论文用AM1、MNDO、MINDO/3（主要是AM1）和INDO/S-CI半经验分子轨道方法对水杨酸衍生物系列——水杨酸、水杨酸甲酯、水杨醛、O-羟基乙酰苯酮、水杨酰胺和3-羟基吡啶酰胺（6种异构体和2-3种阴离子）；2-（2'-羟基-5'-甲基苯基）苯并三〓唑（4种异构体）；2-（2'-羟基苯基）苯并咪唑（3种异构体和3种阴离子）；2，5-二间氮杂氧茚氢醌（3种异构体）；2-（2'-羟基苯基）间〓杂硫茚（2种异构体）和7-〓吲哚二体（2种异构体）的激发态分子内质子转移反应在以下几个方面进行了较系统的理论研究：几何构型优化和相对稳定性及氢键能首先以水杨酸衍生物系列分子为例，用AM1、MNDO和MINDO/3方法考察了5种分子（每种分子设计6种异构体和2-3种阴离子）的基态几何构型优化，能量、相对稳定性和氢键能计算，通过和实验数据进行比较，AM1方法给出的优化几何构型、稳定性次序、氢键能和O—H。。。O氢键的0—0距离与实验数据吻合最好，MNDO和MINDO/3方法优化的C-C键长偏长，C-O键和O-H键长偏短；对于C-N键长，MNDO和AM1优化结果差别不大，而MINDO/3给出了过短的C-N键长，MNDO和MINDO/3方法给出的有些水杨酸衍生物分子（如水杨酸甲酯和水杨酰胺）异构体的稳定性次序和实验上推测的可存在异构体结果不一致，MNDO和MINDO/3方法给出的氢键能偏低，对其他体系的研究发现INDO方法常常不能得到质子转移产物的优化几何构型，只能得到反应物的优化构型，并且估算的氢键能偏高，大量的计算结果表明AM1方法对本论文研究的激发态分子内质子转移反应体系是适宜和可靠的。

Methods:Of 70 cases with traumatic pelvic fracture associated with injuries of other organ, 16 were treated by lienectomy, 7 were treated by liver repair, 6 were treated by colon operation, 6 were treated by intestine inosculation, 4 were treated by bladder fistulatome, 10 were treated by ligation of internal iliac arteries, 1 was treated by half-pelvic excision, 4 were treated by angioembolization, 5 were treated by conduction.

目的：探讨外伤性骨盆骨折合并其他部位伤的诊治方法及最佳适应证方法：对71例外伤性骨盆骨折合并其他部位伤的病例进行不同方法的诊治：脾切除16例，肝修补7例，结肠手术6例，小肠修补吻合6例，膀胱造瘘4例，髂内动脉结扎10例，半骨盆离断术1例，髂血管探查取栓4例，胸腔闭式引流5例。

Methods:of 70 cases with traumatic pelvic fracture associated with injuries of other organ, 16 were treated by lienectomy, 7 were treated by liver repair, 6 were treated by colon operation, 6 were treated by intestine inosculation, 4 were treated by bladder fistulatome, 10 were treated by ligation of internal iliac arteries, 1 was treated by half-pelvic excision, 4 were treated by angioembolization, 5 were treated by conduction.results: totally 11 cases were dead, death rate was 15.5%.

对71例外伤性骨盆骨折合并其他部位伤的病例进行不同方法的诊治：脾切除16例，肝修补7例，结肠手术6例，小肠修补吻合6例，膀胱造瘘4例，髂内动脉结扎10例，半骨盆离断术1例，髂血管探查取栓4例，胸腔闭式引流5例。结果：全组死亡11例，死亡率15.5%。1例死于肺栓塞，1例死于ards，1例死于mof，8例死于出血性休克。

Some tracks are straight off of soundtrack albums, and others are soundtrack tunes, lifted from jazz albums -- but altogether, they make for a wonderful collection of work -- with titles that include "Silvia" by Lalo Schifrin,"Mutiny On The Bounty" by Bob Brookmeyer,"The Bobo" by Francis Lai,"Bossa Nova In The Restaurant" by Ken Thorne,"Bossa Des Teurs" by Vladimir Cosma,"I Like The Look" by Henry Mancini,"Mr Lucky" by Jack Wilson,"Deiter's First Mistake" by Quincy Jones,"Naked City" by Laurindo Almeida, and Dax Rides by Antonio Carlos Jobim.

一些轨道是直线从配乐专辑，和其他音乐曲调，解除爵士乐相册-但总的来说，他们的精彩收集工作-与游戏，包括&西尔维亚&的拉罗塞富林，&哗变赏金&由Bob Brookmeyer说：&波波&弗朗西斯来，&波萨诺瓦的中餐馆&的肯斯索恩，&博萨辅员&由Vladimir科斯马，&我喜欢看&的亨利曼西尼，&他不错&的杰克威尔逊& Deiter的第一个错误&的昆西琼斯，&裸体之城&的劳林阿尔梅达和达克斯游戏机安东尼卡洛斯裘宾。

By using two-stage separations indicates that, the content of total ketones compound by one stage separation is 12.14%, far lower than 54.95% by two-stage separations, and the content of pulegone and menthone increases from 3.37% and 3.46% by one-stage separation to 19.34% and 22.95% by two-stage separations respectively. But the content of the long chain unsaturated fatty acid and its ester ingredient reduces from 41.58% and 18.88% by one stage separation method to 29.06% and 9.86% by two-stage separations method respectively, also the phenol compound by two-stage separations method can been separated completely.

对荆芥挥发油采用两级分离的研究表明：酮类化合物的总含量由二级分离的54.95%远高于一级分离的12.14%，其中胡薄荷酮和薄荷酮的含量分别由一级分离的3.37%和3.46%增加到二级分离的19.34%和22.95%，而长链不饱和脂肪酸及其酯类成分含量分别从一级分离法的41.58%和18.88%降低到二级分离法的29.06%和9.86%，且二级分离法已完全除去酚类化合物。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

By a man's finger-nails by his coat-sleeve by his boots by his trouser-knees by the callosities of his forefinger and thumb by his expression by his shirt-cuff - By each of these things a man's calling is plainly revealed.

一个人的手指甲、衣袖、靴子和裤子的膝盖部分，大拇指与食指之间的茧子、表、衬衣袖口等等，不论从以上所说的哪一点，都能明白地显露出他的职业来。

By a man's finger-nails,by his coat-sleeve,by his boots,by his trouser-knees,by the callosities of his forefinger and thumb,by his expression_r_r_r,by his shirt-cuffs,by each of these things a man's calling is plainly revealed.

一个人的手指甲、衣袖、靴子和裤子的膝盖部分，大拇指与食指之间的茧子、表情、衬衣袖口等等，不论从以上所说的哪一点，都能明白地显露出他的职业来。

By a man's finger-nails, by his coat-sleeve, by his boots, by his trouser-knees, by the callosities of his forefinger and thumb, by his expression_r_r, by his shirt-cuff - By each of these things a man's calling is plainly revealed.

一个人的手指甲、衣袖、靴子和裤子的膝盖部分，大拇指与食指之间的茧子、表情、衬衣袖口等等，不论从以上所说的哪一点，都能明白地显露出他的职业来。

By a man's finger-nails, by his coat-sleeve, by his boots, by his trouser-knees, by the callosities of his forefinger and thumb, by his expression_r, by his shirt-cuff - By each of these things a man's calling is plainly revealed.

一个人的手指甲、衣袖、靴子和裤子的膝盖部分，大拇指与食指之间的茧子、表情、衬衣袖口等等，不论从以上所说的哪一点，都能明白地显露出他的职业来。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。