查询词典 by-time

The apoptosis was evaluated by AQ-stained fluorescin, DNA ladder by gel electrophoresis and subdiploidy by propidium iodide-stained flow cytometry. The level of lipid peroxide under KI treatment was determined by measuring the production of thiobarbituric acid reactive substances.

取健康的猪甲状腺细胞培养24h后，加入不同浓度的碘化钾，观察细胞的形态改变；琼脂糖凝胶电泳、流式细胞术分析细胞DNA的降解；MDA法测定细胞膜的脂质过氧化程度。

Fluxible PAA solution is deposited on quartz glass substrate by MicroPendirect-writing deposition process and imidized to polyimide sacrificial layer in oven;Au paste structural layers are fabricated by MicroPen direct-writing deposition technology,and finally released by oxygen plasma dry etching process.Technological procedures and mechanisms of the LMM direct writing technology arestudied systematically by theory analysis and experiments.

在设备和材料体系基础上，系统研究了基于激光-微笔／微喷直写工艺集成制造MEMS牺牲层、结构层及导电电极图形的工艺机理和质量控制规律，结果表明：(1)笔头内径、笔头与基片间距、驱动气压和直写速度是影响微笔直写沉积膜层线宽、膜厚及表面形貌的主要工艺因素。

Fluxible PAA solution is deposited on quartz glass substrate by MicroPendirect-writing deposition process and imidized to polyimide sacrificial layer in oven;Au paste structural layers are fabricated by MicroPen direct-writing deposition technology,and finally released by oxygen plasma dry etching process.Technological procedures and mechanisms of the LMM direct writing technology arestudied systematically by theory analysis and experiments.

在设备和材料体系基础(来源：A70BC论0c文网www.abclunwen.com)上，系统研究了基于激光-微笔／微喷直写工艺集成制造MEMS牺牲层、结构层及导电电极图形的工艺机理和质量控制规律，结果表明：(1)笔头内径、笔头与基片间距、驱动气压和直写速度是影响微笔直写沉积膜层线宽、膜厚及表面形貌的主要工艺因素。

The sand dunes analyses data have shown they mainly consist of well sorted, symmetry skewness and find sands, and the kurtosis peak is narrow or very narrow. There are abundant impacts of mechanic process on the surface of quartz grains. The traces of eolian process often superpose on the traces stamped by river and beach processes. The dish--shaped concavities and mechanic pits are the most common surface textures, but the barchan concavities and curved grain edges are indicated the marks by eolian environment. The heavy minerals assemblages of coastal dunes are dominated by relatively high specific gravity which have stable chemical properity suggesting the mordern high dynamic environments of coast area. The depositional structures of coastal dunes are typified by corss--bed sets composed of steep foreset laminates and gentle windward dipping sets. It also contains some shell fragments and strong worn foraminiferal tests that are impossibly found in inland desert. On the other hand, the sandy deposits of the continental shelf of East China Sea are with wide range of grains, and the depositional pattens are quite complicated.

海岸沙丘是晚全新世以来相对稳定的海平面背景下的海岸带风成堆积，其沉积特征是以细沙为主，分选好，搬运方式以跃移占绝对优势，偏度上近对称，峰态窄到很窄；石英颗粒表面机械作用形态丰富，风成作用形态叠加于河流和海滩过程形态之上，碟形坑和撞击麻点是最为常见的表面形态，而新月形坑和曲脊是其指示性的特征标志；沙丘沙重矿物以化学性质稳定、比重较大的种类为主，反映了海岸带目前的高能动力环境；海岸沙丘沙的沉积构造以高角度前积层和低角度顶积层组成的交错层理为典型特征，同时还含有少量内陆沙漠所没有的少量贝壳碎屑和强烈磨损的有孔虫壳体。

By means of cephalometric analysis and computeraid operative simulation, the best surgical proposal were gained. Results 15 cases were treated by TMJ reconstruction、 Jaw advancement by transplanted with bone graft and ramus sagital osteotomy, and genioplasty, 3 of the cases treated secondarily by Le fort I osteotomy.

采用电脑辅助的诊断和手术模拟及预测系统，获得充分扩展口咽和纠正颌面畸形的最佳手术方案，以TMJ重建、牵引成骨和正颌外科方法治疗患者。

The data byχ~2 were consistent with the separation ratio as 1: 2: 1 χ~2=4.028＜χ_(0.05,2~2=5.99, which further conformed that glandless character was controlled by a incomplete dominance gene, Gl_2~e. 2. SSR markers every about 5cM were selected in the chromosome 12 of our lab genetic linkage map to screen the parent plants. The markers with the polymorphism were used to amplify the DNA of 210 F_2 segregating plants selected randomly and recorded the marker genotypes. After eliminating the segregation distortion markers byχ~2 test, other SSR markers were detected by linkage analysis.

二、参照本实验室海陆种间遗传连锁图谱第12染色体上的SSR标记，平均每5cM左右选择一个标记进行亲本多态性的筛选，筛选出具有多态性的标记位点进一步扩增随机抽取的210个F_2群体单株的DNA，鉴定每个个体的标记基因型。X~2检测剔除偏分离标记后进行连锁分析，发现Gl_2~e基因位于CIR362与NAU5079之间，进一步参考图谱上位于CIR362与NAU5079之间的标记加密图谱，最终将其定位在CIR362和NAU2251b、NAU3860b、STV033之间。

The synthesized PCU was then characterized by infrared spectroscopy, differential scanning calorimetry, thermogravimetric analysis, small-angle X-ray scattering, and tensile strength measurement. The IR showed that the polymer synthesized by the above two methods both have the polycarbonate-urethane structure and the tensile measurement showed good mechanical performance of the polymer. The DSC and TGA data showed that there is an obvious soft-hard glass transition area, which indicated the existence of the micro-phase separation structure. The extent of the phase separation was affected by the ratio of starting materials, the molecular weight of PCD, and the polymerization method. The decomposition upon heating was observed in two steps. SAXS result showed that the radius (r_2), the distance between domains (d_2), and gyral radius (R_2) of hard segment micro-domain were fairly affected by the starting materials ratio and soft segment content.

IR结果表明两种方法合成的聚合产物均具有聚碳酸酯聚氨酯的结构；拉伸实验表明聚合产物具有良好的力学性能；DSC、TG结果表明，聚合物呈现出较为明显的软硬段玻璃化转变区，表明存在微相分离的结构，相分离的程度受原料配比、软段分子量、聚合方法的影响，其热分解分为明显的两个阶段； SAXS结果表明，聚合物原料配比、软段含量的变化对硬段微区回旋半径R_2，微区间距d_2，微区半径r_2影响不大其值分别为9.5(A|°、29、7.4，但对软段的微区半径及间距影响较大，随着软段含量的增大，软段微区的半径r1和间距d1都增大。

Results The peak of antibacterial activity appeared at 36-48 hafter induced by four inducements (Because many M.domestica larvae pupated,we did not observe the peak of antibacterial activity by heat-shock). The antibacterial activity of antibacterial proteins by S.aureus was best,and the antibacterial activity of antibacterial proteins by heat-shock was better than control group.

结果 各诱导组诱导家蝇幼虫产生抗菌蛋白达最大活性时间为诱导后36～48h(热诱导后家蝇幼虫很快化蛹，未观察抗菌蛋白产生高峰时间)；金黄色葡萄球菌诱导后的抗菌蛋白抗菌活性最佳，热诱导后的抗菌蛋白抗菌活性也较未诱导组好。

Human umbilical venous endothelial cells were cultured, and were exposed to different concerntrations of HCY with/or not with CuSO4; Cell counting of detached cells were performed with a hematocytometer; the cell survival was monitored by MTT assay and the cellular injury was evaluated by LDH release; Hoechst 33258 staining was used to observe nuclear morphological changes and the apoptosis was measured by DNA agarose gel electrophoresis ;the percentage of apoptosis was quantified by flow cytometry.

体外培养人脐静脉血管内皮细胞，用不同浓度的HCY伴／不伴生理浓度Cu~(2+)与内皮细胞作用；细胞计数板检测脱落细胞率；MTT法及乳酸脱氢酶释放率的测定来衡量细胞的存活与损伤；Hoechst 33258荧光染色观察细胞核形态变化和DNA琼脂糖凝胶电泳检测细胞凋亡，并采用流式细胞术定量测定细胞凋亡率。

A diverse range of effects:on protein solubility and secretion was observed,and these effects were highly dependent on the particular chaperone.In the host strain over-expressing chaperone SecB harboroing pSecB,the amount of total periplasmic proteins was increased by about 71％and the activities of alkaline phosphatseand GL-7-ACA acylase(GL7A)were increased by about 54％and l.5-fold respectively.In the host strain over-expressing chaperone GroEL bearing pGroEL,the amount of total periplasmic proteins was increased by 52％,and the activity of penicillin G acylasewas in creased by about 76％;about 90％of Hexameric Calcitonin(Cal6)inclusion body and l5％of MS2interleukin3(MS2-HIL3)inclusion body became soluble respectively.All the data were compared with the parental strains un-bearing plasmdis pSecB or pGroEL.

在过量表达SecB的宿主菌中，周质空间分泌蛋白总量较对照组提高了约71％，GL-7-ACA酰化酶在周质空间酶的活力较对照组提高了约1.5倍，碱性磷酸酯酶在周质空间酶的活力较对照组提高了约54％；在过量表达GroEL的宿主菌中，周质分泌蛋白总量较对照组提高了约52％，青霉素G酰化在周质空间酶的活力较对照组提高了约76％，鲑鱼降钙素六聚体的可落性组分的比例由原来的45％增加到约90％，而MS2-人白介素-3融合蛋白的包涵体有约15％转变为可溶性组份。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。