查询词典 by-time

The pcDNA〓-apoE〓 plasmid was transfected to SK-N-SH Neuroblastoma cell by liposome. It could be screened by G〓. The immunohistochemical assay was shown that neuroblastoma cell transfected by pcDNA〓-apoE〓 expressed the recombinant apoE protein. The intracellular expressed recombinant protein could inhibit the death of neuroblastoma cell induced by beta amyloidal peptides 25-35 fragment.

用pcDNA〓—apoE〓真核表达载体与脂质体共转染神经成纤维胶质瘤细胞，用G〓选择压力筛选，细胞免疫荧光检测表明，pcDNA〓—apoE〓转染的神经成纤维胶质瘤细胞表达了apoE〓重组蛋白，这种内源性的apoE〓重组蛋白对25μM Aβ25—35多肽诱导的神经成纤维胶质瘤细胞的死亡具有拮抗作用。

DCs acquired by our reformed methods express both CD83 and CD 14 molecules highly, and have a higher density than other domestic reports. The higher TNF in DCs culture medium of HC patient suggests DCs in patient still have antigen presenting ability and by optimization the culture medium would improve its presenting ability and have a potential value in design and application individual vaccine. Although antigens pulsed DCs have a decrescent antigen presenting ability but BCG HSP70 could induce its mature and improve its presenting ability. Suggests BCG HSP70 would be a useful mature inducer. Lymphocytes primed by DCs based HC vaccine have the specific cytotoxicity against HCC lines. The CTL after freezing and anabiotic could prophylaxis and therapy HC xenograft on nude mouse. The results also suggests that CD4〓 lymphocytes play a important role in HC with a good differentiation and would be useful in treatment this kind of HC. After being activated by Peptide LLNQHACAV of hAFP and apoptotic HCCs pulsed DCs respectively, the culture medium of activated lymphocytes both contains a high level Th1 cytokines IL-12 and TNF. Primed lymphocytes appeared a characteristic of NK cells. DCs not only inhibited the growth of human HCC and other cancer cells in vitro but also prevented the growth of HC xenograft on nude mouse in vivo. There are at least three kinds of mechanism playing important role in DC based vaccine ,there are inhibition of DCs, HC specific CTL and cytokines pathway.

诱导出的DC共同表达CD83和CD14分子，CD83分子表达明显高于国内报道；肝癌患者DC培养上清中TNF水平高于健康人，提示肝癌患者DC仍具一定的抗原呈递能力，适当调控可使其行使APC功能，以期在肝癌个体化疫苗中发挥作用；DC负载肝癌可溶性抗原后，抗原呈递能力降低，BCG HSP70可促进DC成熟，增加其抗原呈递能力，预示BCG HSP70有可能成为促进DC活化和成熟的另一重要分子；肝癌DC疫苗在体外诱导肝癌特异性淋巴细胞，活化的淋巴细胞在体外对肝癌细胞的杀伤以特异性CTL为主，同时分泌较高水平Th1型细胞因子IL-12和TNF，并抑制4种肝癌细胞生长；冷冻复苏后的肝癌特异性淋巴细胞可以预防和抑制人肝癌裸鼠皮下移植瘤，提示DC负载肝癌可溶性抗原后诱发的MHC-Ⅱ类限制性CD4〓T细胞有可能在分化程度高的肝癌治疗中发挥作用；用DC和HLA-A2〓DC分别负载凋亡肝癌细胞和hAFP218-226位LLNQHACAV HLA-A2限制性九肽，在体外诱导肝癌特异性淋巴细胞，活化后的CTL细胞分泌较高水平的Th1型细胞因子IL-12和TNF，并具较强杀伤活性，此CTL同时具备NK细胞特征；DC对肿瘤细胞的抑制作用可能是通过吞噬实现的，Fas-L在DC抑制中也起一定作用；DC对人肝癌裸鼠皮下移植瘤的抑制率为97％；在肝癌DC疫苗的作用中，至少联合3种以上机制，即通过DC的直接作用、肝癌特异性CTL和细胞因子途径直接或间接地杀伤和抑制肝癌细胞。

The types of the images of women created by ShiZhecun contains the type of chastity imagined by man"s personally thought, the type of tradition imagined by men"s collective unconscious, the type of deregulation in a androcentric society, the type of stunner which were pursued by men and defeated them, the type of which came from mens anxiety and fear.

这些女性形象主要包含了以下几个类型：男性个人化想象中的贞节女性、男权社会集体性规约下的传统女性、男权社会中的违规女性、既是男性的欲望追逐对象又成为男性迷失标记的尤物女性以及男性焦虑恐惧心理下所产生的妖魔女性。

Methods The constriction induced by norepinephine (NE, 10-8-10-4 mol/ L) and diastole induced by acetylcholine (Ach, 10-8 -10-4 mol/ L) in thoracic aortic ring with angiosteosis induced by vitamin D3 plus nicotine in rats were recorded by BL-Newcentrary multichannel physiologic recorder.

维生素D3(VitD3)和尼古丁诱导大鼠血管钙化模型，通过BL-Newcentrary型多导生理记录仪，观察钙化大鼠离体胸主动脉环对去甲肾上腺素(norepinephine，NE)(10-8～10-4mol/L)和乙酰胆碱(acetylcholine，ACh)(10-8～10-4mol/L)引起的收缩和舒张反应。

S. bumalda from the Xiaochang County in Hubei Province was sunned to 90% dryness and dried to constant weight in the oven at 105 ℃, then grinded and its protein content was detected by micro-kjeldahl method, the soluble sugar content was detected by anthrone colorimetry, the crude fat content was detected by soxhlet extraction method, total ash content was detected by the method of burning with high temperature.

方法］将晒至9成干的湖北孝昌县珍珠花于105℃的烘箱中干燥至恒重，然后粉碎，凯氏定氮法测定其蛋白质含量，蒽酮比色法测定其可溶性糖含量，索氏抽提取法测定其粗脂肪含量，高温灼烧法测定其总灰分含量。

Leukocyte proteins concentrations were determined with Bradford Method. Phosphoproteins in the collected leukocyte proteins were enriched by BDTM Phosphoprotein Enrichment Kit. Finally the enriched phosphoproteins were separated by SDS-PAGE and dyed by argentation, and the electrophoresis map of SDS-PAGE was analyzed by Scion Image Tool.

刺激组加入表皮生长因子(40 μg/L)；抑制剂组加入细胞外信号调节激酶特异性抑止剂UO126 （10 μg/L）和表皮生长因子（ 40 μg/L）；对照组加入等量的培养基，用Bradford法测定细胞蛋白质含量，BDTM Phophoprotein Enrichment Kit分离富集白细胞磷酸化蛋白质，SDS-PAGE分离磷酸化蛋白质，银染法染色，采用Scion Image软件分析电泳结果。

Methods S. epidermidis atlE gene expression level at different phases was detected by RT-PCR, and the accumulation of extracellular DNA at different phases was assessed by spectrophotometric measurements of light absorbance by DNA; DNase Ⅰ was used to study the function of extracellular DNA in biofilm formation and primary attachment. The effects of atlE gene deletion on initial adherent capacity, biofilm formation and extracellular DNA release were studied by construction of atlE deletion mutant via homologous recombination.

采用RT-PCR法检测表皮葡萄球菌atZE基因在不同时期的表达水平，用紫外分光光度计检测DNA的方法检测了相应时期的胞外DNA的释放量，并用DNA酶研究表皮葡萄球菌胞外DNA在生物膜形成和起始黏附中的作用；采用ρBT2质粒同源重组敲除的方法构建了表皮葡萄球菌1457的atlE基因突变株，研究atlE基因敲除突变对起始黏附能力、生物膜形成及胞外DNA释放能力的影响。

The procedure is as follows: First, the haze area is recognized by using band statistic values, Second, the remote sensing image is classified by using unsupervised classification, then the aerosol optical depth distribution is calculated by comparing the radiation values in haze and clear areas, Finally, the image can be atmospherically corrected by using MODTRAN model.

首先，结合SPOT 5各波段的统计特征，从影像上识别出模糊区；然后，对影像进行非监督分类，对同一分类区内像元在模糊区和清晰区的辐射值进行比较，计算出气溶胶空间分布；最后，将气溶胶分布输入MODTRAN模型中，对影像进行大气校正。

We hold Immigration Representation License issued by CSIC, so our Representation for our loyal clients is legal and authentical, also covered by Error and Omission Liability Insurance. Our professional practice is bound by the Code of Ethics of CSIC, also bound by the Canadian Laws and applicable International Laws.

雷格拥有CSIC移民牌照，所以移民签证客户案例代理均为合法，并被加拿大移民局驻外使领馆承认，所以移民签证客户案例均受加拿大移民代理职业过失保险承保，并收CSIC协会法律及国际法制约。

Deviation or delay in prosecuting the voyage contemplated by the policy is excused Where authorized by any special term in the policy; or Where caused by circumstances beyond the control of the master and his employer; or Where reasonably necessary in order to comply with an express or implied warranty; or Where reasonably necessary for the safety of the ship or subject-matter insured; or For the purpose of saving human life, or aiding a ship in distress where human life may be in danger; or Where reasonably necessary for the purpose of obtaining medical or surgical aid for any person on board the ship; or Where caused by the barratrous conduct of the master or crew, if barratry be one of the perils insured against.

船舶在完成保险单载明的航程中，下述绕航或延迟是许可的：保险单中特殊条款授权者；船长或其雇员不能控制的情况造成的；为遵循明示或默示保证所合理必要的；为船舶或保险标的的安全所合理必要的；为救助人命或为救护有人命危险的遇难船舶；为船上任何人员合理地及时得到内，外科治疗所合理必要的；如果&船长，船员欺诈恶行&是承保风险，由于船长或船员的欺诈恶行造成的。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐