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Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%,0 and 38% respectively in sorted GPA positive cells of 3 samples by FISH,and the results of GPA+ cells of the 3 cases by PCR were positive,negative and positive respectively,which were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16～24周的孕妇血中分离出NRBCs，并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果，3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%；其PCR结果，分别为阳性、阴性、阳性，并在引产后得到性别证实。

Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%0 and 38% respectively in sorted GPA positive cells of 3 samples by FISHand the results of GPA+ cells of the 3 cases by PCR were positivenegative and positive respectivelywhich were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16～24周的孕妇血中分离出NRBCs，并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果，3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%；其PCR结果，分别为阳性、阴性、阳性，并在引产后得到性别证明。

Abortus, amplify BP26 gene by PCR and clone to pMD18-T simple vector. Identify the constructed recombinant plasmid pMDBP26 by sequencing, then subclone to vector pET-28a. Transform the constructed recombinant plasmid pETBP26 to E. coli BL21(DE3) for expression under induction of IPTG. Purify the expressed product by histidine-binding resin column chromatography and identify by Western blot.

提取布鲁氏菌基因组DNA，用PCR扩增出BP26基因，并克隆到pMD18-T载体上，测序正确后，再亚克隆至pET-28a载体上，转化到大肠杆菌BL21(DE3)中，用IPTG诱导表达，经组氨酸结合树脂柱纯化，并对纯化后的表达产物进行Western blot鉴定。

The plasma density scale length decided by main laser pulse in the experiment is suitable for Vacuum Heating, and it is the domiant process for hot electron generation. From the scaling law about hot electron temperature given by VH model, the hot electron measured by experiment was reasonable, the temperature was higher than the temperature given by Rcsonant Absorption scaling law.

从真空吸收给出的超热电子温度的定标率来看，实验中测量得到的超热电子温度是合理的。X射线是各向同性的，X射线产额随入射激光强度的降低而减小，当强度小于10〓W/cm〓时，不再有硬X射线产生。

The bending load by method of hand lay-up was better than one by method of SCRIMP, the fatigue strength by method of hand lay-up was better, the impact behavior by method of hand lay-up was also better, but ductility by method of SCRIMP was better. The test data may useful for structure design of marine engineer.

同时在冲击试验从能量的观点来出发，从实验方法，到比较单位面积冲击能量、单位宽冲击能量，依照4种不同的冲击能量来做一个比较，以知道哪种工法可以吸收较多的冲击能量，以及何种工法制做出的试片属於脆性破坏，剩余强度较高。

Renewing the decision taken by our predecessor of happy memory, Innocent, we decree that the ordinances made by the heresiarchs Octavian {2 } and Guido {3 }, and also by John of Struma {4 } who followed them, and by those ordained by them, are void; and furthermore that if any have received ecclesiastical dignities or benefices through the foresaid schismatics, they are to be deprived of them.

革新所作出的决定感到高兴我们的前任的记忆，无辜的，我们的法令，该法令所作的heresiarchs 屋大维（ 2 ）和 Guido （ 3 ），也由 John 的甲状腺肿（ 4 ）谁跟著他们，并通过这些晋牧他们，都是无效的；而且，如果有任何收到教会尊严或benefices通过前述schismatics ，他们被剥夺他们。

The author has limited the topic to only five of the new approaches:1 the reaction to the Romantic criticism by the historical critics, 2the attacks on the "God of Idolatry" by the unbradleyan critics, 3the revelation of Shakespeare s temperament by linguasymbolic critics, 4the study of Shakespeare s motivation for his dramatic creation by the psychoanalytic critics, and 5the selfportrait of Shakespeare by Rowse.

他们以纯粹审美的观点来研究剧作中人物的思想感情和一言一行，企图从中发现莎翁深刻的、首尾连贯的戏剧意图，并且根据人物的内心规律来解释人物的一切表现。这些有着敏锐批评洞察力的浪漫派莎评家们依靠自己的艺术直觉把握莎士比亚，揭示了莎士比亚在戏剧手法和人物刻划方面的惊人天赋；但这种莎士比亚评论最后发展成了莎士比亚崇拜。

But as my mother often said, ''They asked for trouble'' —by the way they make their arguments, by their puzzlingly shoddy scholarship, by what they emphasize and de-emphasize, by what they leave out and by writing on this sensitive topic without doing extensive interviews with the lobbyists and the lobbied.

他们制造的这些争议，他们偷换的概念，他们强调的和忽略的，他们轻率的未经调查访问就提出的那些关于游说组织的敏感论调，所有这些就像我妈妈说的那样，&他们在自找麻烦&。

When 〓 is the family of bipartite graphs,the 〓 capacityand the 〓 valency of a graph G are referred to as the biparticitydenoted by β(Gand the bivalencydenoted by 〓(Gof G respectively;when 〓 is the family ofgraphs whose components are complete bipartite graphs,the 〓 capacity and the〓 valency of a graph G are referred to as the complete biparticitydenoted byc(Gand the complete bipartite valencydenoted by 〓(Gof G respectively;andwhen 〓 is the family of star forests,the 〓 capacity and the 〓 valency of a graphG are referred to as the star arboricitydenoted by st(Gand the star valency ofG(denoted by 〓)respectively.

以v记图G的完全二部分解中顶点υ所关联的π中完全二部图的个数，记〓这里π取遍G的所有完全二部分解。我们称υ为G的完全二部次。对一般的图，当图的阶数充分大时，P。〓和L.Pyber给出了υ的一个上界，即，〓。F.R.K.Chung等人及Z.Tuza的结果说明这一上界是不可改进的。对υ的具体值，即使是对某些特殊的图类，结果也知之甚少。P.C.Fishburn和P.L.Hammer给出了一个相关的结果：当n≤16时，〓，（在υ的定义中，以完全二部覆盖替代完全二部分解，即得〓。同时他们提出了下述公开问题。问题1。〓=？

Matters have gone far enough, with the Pingkiang Massacre in Hunan, the Chuehshan Massacre in Honan, the attack on the Eighth Route Army by Chang Yin-wu in Hopei, the attack on the guerrillas by Chin Chi-jung in Shantung, the ruthless slaughter of between five and six hundred Communists by Cheng Ju-huai in eastern Hupeh, the large-scale assaults on the garrison forces of the Eighth Route Army by the Central Army in eastern Kansu and, more recently, the tragedy in Shansi where the old army attacked the new army and invaded positions held by the Eighth Route Army.

演变所极，湖南则有平江惨案，河南则有确山惨案，河北则有张荫梧进攻八路军，山东则有秦启荣消灭游击队，鄂东有程汝怀惨杀共产党员五六百之众，陇东有中央军大举进攻八路驻防军之举，而最近山西境内复演出旧军攻击新军并连带侵犯八路军阵地之惨剧。

Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势，并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。