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不过这里也借此诚心忠告所有站长，咱们还是规矩做正规站，按部就班来优化推广，用户体验是第一，优化排名以原创优质内容为王，认真用心去做就好了，不要歪门邪道的做垃圾站或者为了推广被收录不择手段，那样即使短期见效，也不会多久就会被K掉的，不仅被搜索引擎K掉，更重要是被用户K掉了，站关了可以重建，可人的信誉良心丧失了，那做什么都不会长久的，更不会被人认可的，俺是这么认为的。

And therefore, whatever form the common-wealth is under, the ruling power ought to govern by declared and received laws, and not by extemporary dictates and undetermined resolutions: for then mankind will be in a far worse condition than in the state of nature, if they shall have armed one, or a few men with the joint power of a multitude, to force them to obey at pleasure the exorbitant and unlimited decrees of their sudden thoughts, or unrestrained, and till that moment unknown wills, without having any measures set down which may guide and justify their actions: for all the power the government has, being only for the good of the society, as it ought not to be arbitrary and at pleasure, so it ought to be exercised by established and promulgated laws; that both the people may know their duty, and be safe and secure within the limits of the law; and the rulers too kept within their bounds, and not be tempted, by the power they have in their hands, to employ it to such purposes, and by such measures, as they would not have known, and own not willingly.

所以，无论共同体处于什么形式之下，统治的权力都应当由公布并被接受的法律而不是由临时的命令和未确定的决议来支配：因为人类一旦将多数的联合权力武装一个或少数人，来迫使人们服从他们突发奇想的过度和无限制的法令，或者服从于此时尚没有确定的尺度来管理和裁判他们行为的无约束的意志，他们将跌入远比自然状态更糟的境遇之中：因为政府所拥有的所有权力，既然仅仅只是为了社会的利益，就不应当是肆意和凭一时高兴的，它应当依建立和公布的法律来行使；不仅人们知道自己的责任，知道在法律的约束之中安全能够得到保证；而且统治者也被限制在他们的边界之内，而不会被他们手中的权力所诱惑，从而雇用这种权力去为人们并不知道和并不情愿的目的服务，并作为这样的手段。

And therefore, whatever form the common-wealth is under, the ruling power ought to govern by declared and received laws, and not by extemporary dictates and undetermined resolutions: for then mankind will be in a far worse condition than in the state of nature, if they shall have armed one, or a few men with the joint power of a multitude, to force them to obey at pleasure the exorbitant and unlimited decrees of their sudden thoughts, or unrestrained, and till that moment unknown wills, without having any measures set down which may guide and justify their actions: for all the power the government has, being only for the good of the society, as it ought not to be arbitrary and at pleasure, so it ought to be exercised by established and promulgated laws; that both the people may know their duty, and be safe and secure within the limits of the law; and the rulers too kept within their bounds, and not be tempted, by the power they have in their hands, to employ it to such purposes, and by such measures, as they would not have known, and own not willingly.

因此，国家无论采取什么形式，其统治权力都应依据业已公布且被接受的法律而不是临时的指令或犹豫不定的决义来进行治理；因为，假若人类用群众的联合的力量来武装一个或几个人，任其随心所欲地迫使人们服从其一时心血来潮所做出的种种过分的、无限制的命令或是毫无节制的、此时尚不得而知的意志，而同时却没有任何固定的措施来引导他们的行为或为之辩护，那么，人类将处于比自然状态远为糟糕的境地；既然政府所拥有的一切权力均旨在为社会谋福利，因而不应专断独行、随心所欲，因此，它必须依据明确规定且公诸于众的法律来行使；这样，不仅人民可以了解他们的义务，在法律范围内得到安全和保障；而且，统治者也可以受到法律的约束，而不致被手中掌握的权力所诱惑，进而利用它达到特定的目的，且采用特定的手段，而这样的目的和手段是他们本来可能不会知道、也不愿承认的。

Bernard of Tuscany and of Lombardy, approved by Alexander VI (1497); the Congregation of Portugal, or of Alcobaca, founded in 1507; the Congregation of the Feuillants, founded by John de la Barriere in 1563, which spread into France and Italy, the monasteries of Italy, however, eventually detaching themselves from those of France to form the Congregation of the Riformati di San Bernardo; the Congregation of Aragon, approved by a Bull of Paul V (1616); the Congregation of Rome, or of Central Italy, created by a Decree of Gregory XV in 1623; the Congregation of Calabria and Lucania, established by Urban VIII in 1633, and to which was united the old Congregation of Flore, which had for its founder Blessed Joachim surnamed "the Prophet".

会众的庆祝圣伯纳德的西班牙，成立由DOM的马丁德巴尔加斯，在1425年，在蒙地附近的锡永托莱多；聚集的圣伯纳德的托斯卡纳和伦巴第，批准由Alexander六（ 1497 ）；众葡萄牙，或alcobaca ，创立于1507年；聚集了feuillants ，成立由约翰德香格里拉barriere在1563年，蔓延到法国，意大利等寺庙的意大利，但最终拆卸自己从那些法国形成聚集了riformati迪圣贝尔纳；聚集的阿拉贡，批准牛市的保五（ 1616年）；聚集罗马，还是意大利中部，创造了一项法令的格雷戈里十五，在1623个；聚集的卡拉布里亚和lucania成立了由市区八，在1633年，并以这是美国旧聚集flore ，这为它的创办人有福了约阿希姆姓&先知&。

Bernard of Tuscany and of Lombardy, approved by Alexander VI (1497); the Congregation of Portugal, or of Alcobaca, founded in 1507; the Congregation of the Feuillants, founded by John de la Barriere in 1563, which spread into France and Italy, the monasteries of Italy, however, eventually detaching themselves from those of France to form the Congregation of the Riformati di San Bernardo; the Congregation of Aragon, approved by a Bull of Paul V (1616); the Congregation of Rome, or of Central Italy, created by a Decree of Gregory XV in 1623; the Congregation of Calabria and Lucania, established by Urban VIII in 1633, and to which was united the old Congregation of Flore, which had for its founder Blessed Joachim surnamed "the Prophet".

该教会的庆祝圣伯纳德西班牙，创办的大教堂马丁的巴尔加斯，在1425年，在附近的蒙锡永托莱多；聚集的圣伯纳德的托斯卡纳和伦巴第大区，批准了亚历山大六世（ 1497年）；圣座葡萄牙，或阿尔科巴萨，成立於1507年；圣座的Feuillants ，成立由John德拉鲁阿巴里耶尔在1563年，它蔓延到法国和意大利，意大利的寺院，但最终脱离自己免受这些法国形成教会的Riformati圣贝尔纳多；圣座阿拉贡，批准了红牛的保五（ 1616年）；的罗马天主教，或中央意大利，创造了一项法令的格雷戈里在1623年十五；圣座的卡拉布里亚和卢卡尼亚设立城市第八於1633年，并是美国的旧教会的花神，它的创办人有福约阿希姆姓&先知&。

The results show that the effectiveness of Route A is better than Route B. After ARB of 7 passes, the microstructure of the specimen by Route A consists of thin elongated fibriform grains with an average size of 470 nm, but that by Route B does compressed grains with an average size of 680 nm. The improvement in tensile strength by Route A is greater than that by Route B. The strengthening mechanism of 1060 commercially pure aluminum by ARB is fine-grain strengthening.

结果显示：路径A的晶粒细化效果比路径B明显；ARB7道次后，采用路径A的试样的显微组织由拉长的细小纤维状晶粒组成，路径B的试样由扁平状晶粒组成；路径A和路径B的试样的平均晶粒尺寸分别为470nm和680nm；路径A的试样的抗拉强度提高程度大于路径B.1060工业纯铝在ARB过程中的强化机制主要是细晶强化。

Bieti lost its habitat continuously in recently decades associated with the extension of rangelands and farmlands, as well as firewood collection etc. The monkey live mainly in primary fir forest and the mixed conifer forest, to evaluate the status of the monkeys' habitat, we employed GIS and RS software to identify the habitat types with five Landsat TM/ETM+ satellite imagery in winter of 1986, 1992, 1997, 2001 and 2006 years respectively. The work resulted in: 1 the size of summer grazing lands, farmlands, and fir forest was 13 100 hm2, 6 400 hm2, and 30 500 hm2 in 2006 respectively; 2 during the past 20 years (1986-2006), the size of fir forest (including primary fir forest and the mixed conifer forest) decreased by 14.6%(5 200 hm2), summer grazing lands and farmlands increased by 47.2%(4 200 hm2) and 14.3％(800 hm2) respectively; and 3, during the past 20 years, the number of firry forest patches increased by 68.4%, the mean size of firry forest patches decreased by 49.3%(from 15.1 to7.6 hm2), the largest patch index of firry forest decreased 54.9%; the patch richness had no change, but the Shannon's diversity index and the Shannon's evenness index increased by 2.7% respectively.

为了评估该物种的栖息地现状和变化情况，我们通过野外调查工作，应用GIS和RS技术，分别解译了1986年、1992年、1997年、2001年和2006年的Landsat TM/ETM+冬季卫星影像，并对解译结果进行了计算和分析，得到了以下西藏种群栖息地的主要结果：1）现有暗针叶林（包括原始针叶林和针阔混交林）面积是30 500 hm2，夏季牧场面积是13 100 hm2，农田面积是6 400 hm2；2）在过去20年间（1986-2006年），暗针叶林面积减少了14.6％（5 200 hm2），夏季牧场面积增加了47.2％（4 200 hm2），农田面积增加了14.3％（800 hm2）；3）在过去20年间，暗针叶林的斑块数量增加了68.4％，平均斑块面积下降了49.3％（从1986年的15.1 hm2下降到2006年的7.6 hm2），最大的斑块指数下降了54.9％；景观丰富度并没有变化，但Shannon多样性指数和Shannon均匀度指数分别增加了2.7％。

METHODS: A model of T cell activation and proliferation was established by stimulated the cells with Con A.T cells were treated with different concentrations of CPT. The expression of CD69, the early marker of CD3(superscript +) T cell activation, was measured by FACS. The proliferation index was determined by carboxyl fluorescin diacetate succinmidyl ester by flow cytometry. The cell-cycle distribution was analyzed by propidium iodide staining.

以ConA刺激小鼠T淋巴细胞，建立小鼠T淋巴细胞活化、增殖的模型，以不同浓度的CPT作用于该模型，流式细胞术检测T细胞早期活化标志CD69分子的表达；以活体染料羧基荧光素乙酰乙酸染色流式细胞术分析CPT在ConA刺激下小鼠淋巴细胞的增殖相关指数；以碘化丙啶染色流式细胞术分析细胞周期的分布情况。

Methods: A model to evaluate lymphocyte proliferation stimulated with a polyclonal activator, concanavalin A, was established by vital dye carboxyl fluorescin diacetate succinmidyl ester labeling technique. Effects of the different doses of anisomycin on the lymphocyte proliferation were estimated by flow cytometry and MTT methods. The propidium iodide labeling technique was applied to assay the effect of the different doses of anisomycin on changes of the lymphocyte cell-cycle stimulated by ConA or by phorbol ester plus Ionomycin. The percentage of the expression level of CD69 and CD25 on the activated lymphocytes was evaluated by fluorescin-conjugated monoclonal antibody double labeling technique.

以活体染料羧基荧光素乙酰乙酸琥珀酰亚胺酯染色，建立在多克隆刺激剂刀豆蛋白A刺激下评价小鼠淋巴细胞增殖的模型，通过流式细胞术和MTT法分析茴香霉素在不同剂量下对淋巴细胞增殖的作用；采用碘化丙锭染色分析茴香霉素对ConA或佛波醇酯加离子霉素刺激的小鼠淋巴细胞周期变化的作用；利用荧光标记的单克隆抗体双染技术和流式细胞术观察茴香霉素对小鼠CD3~+T细胞早期及中期活化标志分子CD69和CD25表达的影响。

Abstract］ Objective To Treating the nano-SiO2 particle by silicon coupling reagent, functionalize the SiO2 by making double bond on its surface. Polymerizing on the surface of nano-SiO2 surface by the method called emulsion polymerization, the nano-SiO2 is covered by polymer to get the PMMA-KH-570-nano-SiO2 complex material. Test the surface structure of the surface of nano-SiO2 by FTIR, and test sort of its mechanical performances.Comparing the treated nano-SiO2 which is directly dispended in the PMMA emulsion with the SiO2 without any treatment, we are trying to find an effective method which can not only increase the dispersion of SiO2, but also improve the mechanical performance of the artificial teeth.

目的 通过硅烷偶联剂KH-570处理SiO2纳米粒子，向其表面引入双键使其功能化，采用乳液聚合的方法在SiO2纳米粒子表面进行接枝聚合，实现高分子对SiO2纳米粒子的表面包覆处理，制得PMMA－KH-570－SiO2纳米复合材料，采用FTIR对SiO2纳米粒子表面结构进行了表征研究，并且测定了它的多种机械性能，与表面处理过的SiO2纳米粒子直接分散至PMMA乳液中制得的复合材料、未添加任何增强物质的空白组进行比较，探索一种能够提高SiO2在基体中的分散性，为义齿复合材料的研究和开发提供理论依据和参考。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。