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The present invention relates to compounds of formula wherein R is hydrogen, lower alkyl, lower alkoxy, halogen or lower alkyl substituted by halogen; R is hydrogen or halogen; or R and R are together with the carbon atom to which they are attached -CH=CH-CH=CH-; R is hydrogen, lower alkyl, phenyl optionally substituted by halogen, or is hetaryl, optionally substituted by lower alkyl; R is hydrogen, lower alkyl, phenyl, benzyl, lower alkenyl, lower alkoxy, phenyloxy, benzyloxy, S-lower alkyl, halogen, CN, lower alkyl substituted by halogen or O-lower alkyl substituted by halogen; R is hydrogen or lower alkyl, aryl is phenyl or naphthyl; n is 1, 2 or 3; m is 1 or 2; and pharmaceutically acceptable acid addition salts and tautomeric forms thereof.

本发明涉及式化合物及其可药用的酸加成盐和互变异构形式，其中R 1 是氢、低级烷基、低级烷氧基、卤素或被卤素取代的低级烷基；R是氢或卤素；或R 1 和R与它们连接的碳原子一起是-CH＝CH-CH＝CH-；R 2 是氢、低级烷基、任选被卤素取代的苯基或者是任选被低级烷基取代的杂芳基；R 3 是氢、低级烷基、苯基、苄基、低级链烯基、低级烷氧基、苯氧基、苄氧基、S-低级烷基、卤素、CN、被卤素取代的低级烷基或被卤素取代的O-低级烷基；R 4 是氢或低级烷基，芳基是苯基或萘基；n是1、2或3；m是1或2。

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

Among the early Fathers the usage was prevalent of designating as dogmas the doctrines and moral precepts taught or promulgated by the Saviour or by the Apostles; and a distinction was sometimes made between Divine, Apostolical, and ecclesiastical dogmas, according as a doctrine was conceived as having been taught by Christ, by the Apostles, or as having been delivered to the faithful by the Church.

其中早期父亲的用法是普遍存在的指定作为教条的理论和道德戒律的教导或颁布的救世主，或由使徒；区分有时之间神圣的， apostolical ，和宗教教条，据作为一个学说被设想为经过教导基督，由使徒，或视为已交付给忠实由教会。

One was the pqsA' positive expression plasmid constructed by cloning the pqsA'-lacZ fusion digested from pYHP441 into miniCTX-1, whose sequence was then integrated into wild type PAO-1 chromosome by biparental mating procedure. The other was pqsA-E operon knock-out plasmid whose sequence between pqsD and pqsE operon was inserted by tetracycline cassette by the site specific insertion mutagenesis strategy and the mutant constructed by biparental mating of S17-1 that harbored the plasmid and pqsA' positive expression mutant.

一种是酶切质粒pYHP441获得pqsA'-lacZ片段后，亚克隆入质粒miniCTX-1中，构建成PqsA'的阳性表达质粒，随后将构建的质粒，通过双亲交配过程整合入野生型铜绿假单胞菌株PAO-1染色体组中；另一种是通过点特异插入诱变策略，将四环素基因盒插入启动子pqsD和pqsE之间，构建的阴性质粒转化入大肠杆菌S17-1株后，和上述pqsA'阳性表达突变株进行双亲交配过程。

A tunneling machine for producing large tunnels in soft rock or wet, clayey, unconsolidated or bouldery earth by simultaneously detaching the tunnel core by thermal melting a boundary kerf into the tunnel face and forming a supporting excavation wall liner by deflecting the molten materials against the excavation walls to provide, when solidified, a continuous wall supporting liner, and detaching the tunnel face circumscribed by the kerf with powered mechanical earth detachment means and in which the heat required for melting the kerf and liner material is provided by a compact nuclear reactor.

&…一个在软岩石，或湿的、粘土状的、疏松的，或巨砾泥土中挖掘隧道的隧洞掘进机，用先进的以上升暖气流进行隧道核心分离，将边界切口熔入隧道面，同时通过使偏倚在隧道壁上的熔融物料在凝固时提供一个连续墙来支持衬垫，形成一个支持挖掘的墙壁；破碎在切口周围划了界限的隧道核心-用有动力的机械土地分离的方法，在隧道核心中，需要如此操作的热力以及衬垫物料由一个小型的核反应堆提供……&

The effect of zeolites ion-exchanged by transition metals on thermal catalytic cracking reaction is studied. The product distributions of the cracking reactions catalyzed by theβor ZSM-5 zeolites exchanged with transition metals are different from that catalyzed by corresponding Hzeolites, which means the mechanism of cracking reactions has varied. Group Ⅷ metals Fe, Co, Ni and Cu, Zn are shown to be of strong catalytic activity of oxidative dehydrogenation, while Ti and Cr are not of oxidative activity. Catalyzed by zeolites or catalyst containing Ag, conversion of thermal catalytic cracking reactions and the yield of ethylene increase while the yield of propylene does not decrease. Silver can not only promote the formation of carbonium ion, but also convert carbonium ion into free-radical via redox reaction. The weak adsorption of olefins on silver reduces the occurrence of hydrogen transfer and dehydrogenation. As a result, the yield of light olefins is favored by silver in the catalyst.

采用过渡金属交换的分子筛作为催化剂，进行催化热裂解制取乙烯的反应，研究发现，在β沸石分子筛和ZSM-5分子筛中引入过渡金属后，催化热裂解反应的产物分布与相应的氢型分子筛相比有了较大的变化，说明过渡金属的加入对于催化热裂解反应的机理具有影响，Ⅷ族金属如Fe、Co、Ni和第Ⅰ、Ⅱ副族Cu、Zn表现出较强的氧化脱氢活性，产物中氢气、焦炭的产率很高，Ti、Cr则未表现出氧化作用：分子筛及催化剂中引入银后，催化热裂解反应的转化率和乙烯的产率有了提高，丙烯产率没有很大的变化，说明银在催化热裂解反应中能够促进正碳离子的生成，又有可能通过氧化-还原作用部分改变反应机理，促进了自由基的生成，并且由于银对烯烃的吸附很弱，其氢转移反应和脱氢、加氢活性比较低，有利于烯烃产率的提高。

Altogether there are seven chapters in this article. Chapter one starts with the historical background caused by the occurrence of the concept of sexual harassing and analyzes the reasons of sexual discrimination which is brought forward by Catharine A Mackinnon when defining the concept of sexual discrimination. Meanwhile, she analyzes the employers' responsibilitiy which appears to be another feature , has a brief presentation on the gradually widened connotation and denotation extension of the American law and a step- by- step-built anti-sexual harassing law system that is learned from by most of the countries. This chapter helps us to have a general understanding of the overseas anti-sexual harassing law system represented by America. Chapter two is about the analysis of the phenomenon of the Chinese sexual harassing. Before the first sexual harassing case appeared in 2001, it doesn't belong to the concept of law.

第一章从性骚扰概念产生的历史背景入手，分析了麦金侬提出性骚扰概念时将性骚扰定性为性别歧视的原因，对性骚扰概念另一特色雇主责任进行了分析，并简述了美国法律通过不断扩大性骚扰概念的内涵和外延，逐步建立起一套符合本国国情并为世界大多数国家所仿效的反性骚扰法律体系，该章使我们对以美国为代表的国外反性骚扰法律体系有一个全面的了解。

ABSTRACT Inhibition mechanism and the relations between inhibition efficiency and molecular structure of inhibition of carbon steel in chlorhydric acid by benzene nitriles whose inhibition is caused by chemical adsorption and quaternary ammonium salts whose inhibition is caused by physical adsorption are mainly studied from microscope through quantum chemistry calculation, and the relations between inhibition efficiency and molecular structure of inhibition of carbon steel in chlorhydric acid by benzene nitriles are studied by making use of model-constructing thought of grey theory in this paper.

本文主要通过量子化学计算，从微观角度研究了化学吸附型缓蚀剂苯腈类化合物和物理吸附型缓蚀剂季铵盐在盐酸溶液中对碳钢缓蚀的缓蚀机理和缓蚀性能与分子结构的关系；并运用灰色理论建模思想，研究了苯腈类化合物在盐酸溶液中对碳钢缓蚀的缓蚀性能与分子结构的关系。其中，通过量子化学计算，从微观角度研究物理吸附型缓蚀剂在盐酸溶液中对碳钢缓蚀的缓蚀机理和缓蚀性能与分子结构的关系，是在尝试着进行；而运用灰色理论建模思想，研究缓蚀剂缓蚀性能与分子结构的关系，是一项具有尝试性和开拓性的工作。

As the DDP group A549DDP cells were treated with IC10 of cisplatin for 48 hours. As the DDP＋Rh2 group A549DDP cells were treated with IC10 of cisplatin and IC5 of gensenoside Rh2 for 48 hours. As the controlling group A549DDP cells were not treated with any kinds of drugs. Changes in cellular morphology were observed by fluorescence microscopy. Peak value apoptosis of A549DDP cells were inspected by flow cytometry. State of PTP was evaluated by ultraviolate spectrofluorometer. Changs of calcium in cells and membrane potential of mitochondrion were determined by flow cytometry. Release of cyt-c from mitochondrion with westton blot Radiative activity of〓Tc-MIBI ingested by cells was measured withγcounter. Results: IC50 of cisplatine to A549 cells was 24uM.

以无毒浓度的Rh2单独作用于A549DDP细胞作为Rh2组，以低效浓度的顺铂单独作用于A549DDP细胞作为DDP组，以无毒浓度的Rh2和低效浓度的顺铂联合作用于A549DDP细胞作为Rh2+DDP组，作用时间为48小时，以不加药物干预作为对照组，Hoechst33258染色荧光显微镜下观察细胞形态的变化，流式细胞仪检测细胞凋亡峰；紫外光分光光度计检测线粒体PTP开放情况，流式细胞仪检测细胞内钙离子浓度及线粒体跨膜电位的变化，Westton blot检测线粒体内细胞色素C释放情况及Caspase-3的活性，γ计数仪检测细胞摄取〓Tc-MIBI放射性活性。

The fundamental imaging features of osteosarcoma were showed bone destruction, periosteal reaction, neoplastic bone and soft-tissue mass. Among 49 cases examined by radiography, osteoblastic pattern sarcoma was found in 19 cases, osteolytic pattern sarcoma was found in 13 cases and mixed pattern sarcoma was found in 17 cases. The detection rates of bone destruction, periosteal reaction, soft-tissue mass, neoplastic bone and Codman triangle by radiography were respectively 61.2%, 95.9%, 71.4%, 73.5%and 27.7%. All 10 cases with CT scan were showed bone destruction, soft - tissue mass and neoplastic bone. The detection rates of periosteal reaction,憇ieve sign挶 and Codman triangle with CT scan were respectively 90%, 90% and 11%. The tiny neoplastic bone and"sieve sign"were showed sensitively by CT, but Codman triangle was showed by radiography superior to CT.32 cases with MRI scan presented the normal marrow was replaced by the tumor, which was showed low to intermediate signal intensity on T1-weighted images and promiscuity signal intensity on T2-weighted images. 7 cases presented hemorrhage and 24 cases presented necrosis.

骨肉瘤基本影像表现为骨质破坏、骨膜反应、瘤骨和软组织肿块。49例X线表现为成骨型19例，溶骨型13例，混合型17例；X线对骨质破坏、骨膜反应、软组织肿块、瘤骨和Codman三角的检出率分别为61.2%、95.9%、71.4%、73.5%和27.7%；10例CT表现均可见骨质破坏、软组织肿块及瘤骨，CT对骨膜反应、筛孔征和Codman三角的检出率为90%、90%和11%；CT对显示细小肿瘤骨和筛孔征最敏感，但对Codman三角的显示不如X线平片；32例MRI表现为骨髓腔内正常的骨髓高信号被肿瘤信号所取代，T1WI呈低～等信号，T2WI呈混杂信号，7例合并出血，24例中央坏死囊变，32例增强扫描均呈不均匀强化及瘤周可见水肿区。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。