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According to the characteristic of silver nanoparticles, their uniform dispersion in reaction media was obtained by surface modification, ultrasonic radiation and the select of emulsion, and then emulsion polymerization system were formed by combination with reactive monomer and initiator. Nano-Ag/Polystyrene core-shell particles were prepared by emulsion polymerization and their microstructure were characterized by TEM, FTIR, XPS, AFM and TG/DTA. The experimental results showed that silver nanoparticles were coated by polymer and the composite particles had typical core/shell structure, and the microlevel combination was realized. Meantime, the form mechanism of nano-Ag/polystyrene core-shell particles was tentatively researched. The conclusion is that the perfect coating was able to obtained by the control of reaction condition.

根据纳米银粒子的特性，借助于表面处理、超声辐照以及乳化剂的选择，使其均匀分散在反应介质中，再与反应单体、引发剂等形成乳液聚合体系；采用乳液聚合方法、包裹聚合方式，制备出纳米银/聚苯乙烯核壳复合粒子，并借助于TEM、FTIR、 XPS、AFM、TG/DTA等分析手段对复合粒子微观结构进行了表征；结果表明：纳米银粒子表面被聚合物层包覆，复合粒子呈明显的核壳结构，实现了微观尺度上的复合；同时，初步探讨了纳米银/聚苯乙烯核壳复合粒子的形成机理，指出：通过控制反应条件，可以使形成纳米银/聚苯乙烯核壳复合粒子的聚合反应方式占主导地位，实现最大限度地包覆。

The independence of words is shown phonologically by the fact that they may be preceded and followed by pauses; orthographically by their separation by means of spaces or punctuation mark; syntactically by the fact that they may be used alone as a single utterance; and semantically by the possibility of assigning to them one or more dictionary meanings.

现代汉语对词的一般定义为：词是语言中最小的、能独立运用的、形体和意义都固定化的造句单位。两种语言中关于词的描述是比较相近的： 1都有稳定的结构形式 2都有相对独立的概念 3是句子的结构要素差异： 1读音上 2书写上构词对比词是语言的基本单位，但并不是最小的有意义的单位，还可以分成更小的单位。

The effect of genistein (0.8 mg/kg) was partially inhibited by L-NAME, or by sodium orthovanadate (50 μg/kg), a potent inhibitor of protein tyrosine phosphatase;(2) genistein also decreased the PP of renal vascular bed in a dose-dependent manner and the effect of genistein was completely inhibited by pretreatment with sodium orthovanadate, but unaffected by L-NAME; and (3) genistein decreased the PP of mesenteric vascular bed in a dose-dependent manner, an effect which was partially inhibited by sodium orthovanadate, but unaffected by L-NAME.

GST的这一效应可被L-硝基精氨酸甲酯部分阻断，预先注射蛋白酪氨酸磷酸酶抑制剂正钒酸钠(50 μg/kg)，可部分抑制GST (0.8 mg/kg)引起的效应；(2)向肾血管灌注环路中直接注射 GST 也可剂量依赖性地降低肾动脉的灌流压，预先注射正钒酸钠可完全抑制GST引起的效应，而L-NAME对此效应没有影响；(3)肠系膜血管灌流环路中注射GST可剂量依赖性地降低其灌流压，这一效应可被正钒酸钠部分抑制，而L-NAME对此无影响。

The effect of genistein (0.8 mg/kg) was partially inhibited by L-NAME, or by sodium orthovanadate (50 μg/kg), a potent inhibitor of protein tyrosine phosphatase;(2) Genistein also decreased the PP of renal vascular bed in a dose-dependent manner. The effect of genistein was completely inhibited by pretreatment with sodium orthovanadate, but unaffected by L-NAME;(3) Genistein decreased the PP of mesenteric vascular bed in a dose-dependent manner, an effect which was partially inhibited by sodium orthovanadate, but unaffected by L-NAME.

GST的这一效应可被L-硝基精氨酸甲酯部分阻断，预先注射蛋白酪氨酸磷酸酶抑制剂正钒酸钠(50 μg/kg)，可部分抑制GST (0.8 mg/kg)引起的效应；(2)向肾血管灌注环路中直接注射 GST 也可剂量依赖性地降低肾动脉的灌流压，预先注射正钒酸钠可完全抑制GST引起的效应，而L-NAME对此效应没有影响；(3)肠系膜血管灌流环路中注射GST可剂量依赖性地降低其灌流压，这一效应可被正钒酸钠部分抑制，而L-NAME对此无影响。

Results: six patients with vagal reflection were cured by intravenous atropine, 2 with pericardial hemorrhage were treated by pericardiocentesis, 1 patient developed hypotension due to occlusion of the mitral valve orifice by the catheter was corrected by shortening the duration of the catheter in left ventricle, the blood pressure of 1 patient with rapid atrial flutter was elevated by intravenous cedilanid, the blood pressure of 1 patient with hypotension of unknown origin was elevated by vasopressor and atropine.

结果：其中迷走神经反射6例，经静脉注射阿托品纠正；心包出血2例，心包穿刺抽液血压至正常；球囊导管阻塞二尖瓣口1例，缩短导管停留在左心室时间，可以避免低血压反应；快速心房颤动1例，使用西地兰减慢心室率，血压回升；原因不明1例，经使用升压药及阿托品后血压改善。

2 Any notice required or permitted hereunder shall be in writing and shall be deemed given as of the date it is: delivered by hand; by overnight or next day delivery; received by Registered or Certified Mail, postage prepaid, return receipt requested; or received by facsimile, as can be presumptively demonstrated by return fax or letter demonstrating successful facsimile transmission; and addressed to the party to receive such notice at the address and/or facsimile telephone number set forth below, or such other address as is subsequently specified to the notifying party by the receiving party in writing.

11.2 本协议项下所有通知应当以书面形式为之，并且在下列情况下视为已经交付：专人递送；经由隔日送达业务递送；由挂号邮件或保证邮件方式接受，邮资预付，需要回执；由传真接收，并回传真或信函证明已经成功发送；通知应当按照下列地址送交当事人，或按照下列电话号码传送，或者按照接受一方最近书面通知的地址或号码为准

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

The method according to claim 6, for manufacturing a blade wherein a part external to the shell comprises a blade rooting part having at least one attachment bar, in which an end portion for linkage to a hub of said rotor is fitted with a laminate attachment, also comprising thermoplastic composite, and extending said at least one spar bar housed in the shell, the method further comprising the step of producing the elementary piece comprising the at least one spar by producing each attachment bar and the spar bar in the form of a strip of continuous and unidirectional reinforcing fibres which are agglomerated by said thermoplastic matrix, obtained by pultrusion at a melting temperature of the matrix, of several plies of continuous and unidirectional fibres which are agglomerated by said matrix, and, if said rooting part comprises at least two attachment bars, each one of which respectively extends one spar bar, in superimposing parts of corresponding strips forming the spar part housed in the shell, with interposition of at least one ply of continuous and unidirectional fibres which are agglomerated by said matrix between the adjacent superimposed strip parts, and fitting a removable tool, in the shape of a wedge, between parts held spaced from each other of two neighbouring strips, to form two attachment bars of the rooting part, then arranging the strips in a mould, and inserting each linkage end portion of a pultruded strip between two laminate elements of the attachment, to superimpose all the laminate elements of the attachment, closing the mould and compacting its contents under pressure and at a melting temperature of said thermoplastic matrix, and solidifying the matrix by cooling to rigidify the spar thus obtained with the laminate attachment solidly attached to its rooting part.

该方法根据索赔六，制造业刀片其中的一部分，罐壳外部包括一个叶片生根的一部分，拥有至少一个实习酒吧，在其中一结束部分为联系枢纽的说，转子是带有附件的层压板，也包括热塑性复合材料，并延长说，至少有一石酒吧住在蚬壳公司，进一步组成的方法步骤，生产初级一块组成的至少有一个SPAR公司生产每个附件的酒吧和酒吧在SPAR公司的形式，带不断加强和单向纤维是由烧结说，热塑性矩阵，得到的拉挤在熔化温度矩阵，数层的连续和单向纤维是由烧结说，矩阵，如果说，生根部分组成，至少有两个实习酒吧，每个人都分别延长一石酒吧，在叠加部分，相应的带形成的SPAR公司的一部分，居住在蚬壳公司，与干预的至少有一单层连续和单向纤维是由烧结说，矩阵之间的毗邻叠加条形零件，及装修一个可移动的工具，在形状，离间，部分之间举行的间隔从对方的两个邻国带，形成两个附件酒吧的生根部分，然后安排带在一个模具，并插入每个联系年底部分一拉挤带之间的二板的要素扣押，叠加所有层压板的要素扣押，关闭模具和压缩其内容的压力下和在熔化温度说，热塑性矩阵，并巩固矩阵冷却 rigidify该SPAR公司，从而获得与层压板的附着牢固重视其生根的一部分。

The mian works are as follows:(1)By coprecipitation technique and coprecipitation- gel technique the nano zirconia powder was produced, factors affecting the properties of nano zirconia powder such as pH and the content of stabilizator are analyzed;(2)By the new pressless sintering process, the nano zirconia ceramic was produced, factors which can affecting the properties of nano zirconia sinter body such as moulding press and the sinter temperature are analyzed;(3)By the microtherm pressless sintering process, the nano copper added functional zirconia ceramic composite was produced, microscopic dimensionality and electric properties of such sinter body and the factors affecting such sinter body are analyzed;(4)By the self-invented "external albumen coating" technique the zirconia powder was successfully coated by carbon in the corresponding sinter body, which can help solve the traditional aggregation problem to some degree;(5)Based on analyze the characteristics in the nano powder sinter process, the "three-ball sinter model" was proposed, for the relationship between the relative density and porosity of sinter body, by mathematical fitting, such model was successfully verified.

本研究比较系统地研究了纳米氧化锆陶瓷的合成与成型机理，特别是讨论了纳米氧化锆陶瓷的稳定性，为防止纳米颗粒在外场作用下的团聚和长大提供了理论基础和实验数据，具体工作有以下几方面：(1)采用共沉淀法和共沉淀-凝胶法制备了纳米氧化锆粉体，并分析了pH值和稳定剂含量对粉体性能的影响；(2)采用新型无压烧结工艺制备了纳米氧化锆陶瓷，并分析了成型压力和烧结温度对烧结体性能的影响；(3)采用低温无压烧结工艺制备出添加纳米铜的功能性纳米氧化锆复合陶瓷，并分析了烧结体的微观尺度和电学性能的变化以及影响因素；(4)通过自创的&鸡蛋清外敷法&在真空镀膜台上对纳米氧化锆粉体进行包敷碳颗粒的表面处理，并采用真空烧结工艺制得相应烧结体，初步开始解决陶瓷烧结过程中团聚长大这一传统难题；(5)通过分析纳米颗粒在制备烧结体过程中的实际特点，提出了&三球烧结数学模型&，通过引入坯体相对密度和孔隙率的相对关系分析并通过自行编写的数学拟合软件，验证了这一数学模型。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐