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The unoperated sides of the treated animals also served as controls. Six normal rats were treated as normal control group. Three different siRNA plasmid solution containing RC2-Ⅰ, MAFbx-Ⅱ, CON (50μl , 0.8μg/μl)was injected and transfected by electroporation as methods mentioned above, respectively. The changes of RC2 and MAFbx mRNA levels and RC2 protein levels after 3 days were determined by real-time quantitative PCR and Western blot, respectively. On postoperative 2, 3 and 4 weeks, the rate of wet muscle weight preservation, mean diameter of muscle fiber and mean cross-section area of muscle fiber and muscle protein content were checked and then compared between group CON and group RC2 or group MAFbx, respectively. The differences between groups were analyzed by one-way ANOVA. Ultrastructural changes of muscle fiber were observed at 2, 3, 4 weeks postoperation.Results GFP plasmid was efficiently deliverd into muscle by electroporation and robust GFP expression in muscle could be observed more than three weeks. Histology shows that injected plasmid DNA diffuses extensively in muscle tissue.
1、健康雌性SD大鼠18只,随机分为电穿孔组和非电穿孔组,每组9只,制作右下肢趾长伸肌失神经支配模型;EP组为将质粒pEGFP-N1溶液50μl(0.8μg/μl)注射入右趾长伸肌后,立即于两侧腱腹交接处给予电穿孔,电穿孔参数为:电场强度为200V/Cm,脉冲100μs,频率1Hz,施加10次脉冲;NEP组仅质粒pEGFP-N1溶液注射;转染后1、2、3周,荧光显微镜下观察趾长伸肌中GFP的表达情况,转染后1周行Western印迹检测趾长伸肌中GFP蛋白的表达情况,检测和优化体内转染效率。2、健康雌性SD大鼠78只,随机分为失神经对照组、RC2基因治疗组(RC2组),MAFbx基因治疗组,每组24只,制作右下肢趾长伸肌失神经支配模型,余6只为正常组;分别将含CON、RC2、MAFbx基因的siRNA重组质粒注射入趾长伸肌,之后给予电穿孔,方法同上;治疗后3天实时定量PCR和Western印迹检测各组中RC2或MAFbx基因的mRNA和蛋白的表达变化,治疗后2、3、4周检测各组肌湿重维持率、肌细胞直径和肌细胞截面积,肌细胞超微结构变化以及肌纤维中蛋白含量变化。
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His characters are not modified by the customs of particular places, unpractised by the rest of the world; by the peculiarities of studies or professions, which can operate but upon small numbers; or by the accidents of transient fashions or temporary opinions: they are the genuine progeny of common humanity, such as the world will always supply, and observation will always find.
他的人物塑造并不拘泥于只为一部分人所遵循的某个特定地区的习俗,也不局限于一小部分人所从事的特定的研究或职业,也不追随短暂的潮流或暂时的思想观点:他们据有人们一贯具备的、普遍的人性特点。就像世界能永不竭地供应,眼睛能永不停地发现。
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His characters are not modified by the customs of particular places, unpractised by the rest of the world; by the peculiarities of studies or professions, which can operate but upon small numbers; or by the accidents of transient fashions or temporary opinions: they are the genuine progeny of common humanity, such as the world will always supply, and observation will always find .
他是一位自然的诗人,他的作品将人间百态真实地展现在读者眼前。他的人物塑造并不拘泥于只为一部分人所遵循的某个特定地区的习俗,也不局限于一小部分人所从事的特定的研究或职业,也不追随短暂的潮流或暂时的思想观点:他们据有人们一贯具备的、普遍的人性特点。
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His characters are not modified by the customs of particular places, unpractised by the rest of the world: by the peculiarities of studies or professions, which can operate but upon small numbers; or by the accidents of transient fashions or temporary opinions: they are the genuine progeny of common humanity, such as the world will always supply, and observation will always find.
他笔下的人物,不受某特定地区独行的风俗所限制:即,为少数人所从事的研究或行业的特殊面向所局限;不为短暂风尚之偶然事情或人们一时的观感所拘束;他们乃是共同人性之真情流露,诚如此人世到处都有,随时可见者。
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It is shown that polystyrene has been grafted on the surface of nmSiO2 particles and the grafting ratio is about 40%. The surface of nmSiO2 could be treated effectively by KH570in absolute ethanol at 25℃, 48h. The nmSiO2-g-PSt unpurified by toluene appear microsphere in which nmSiO2 particles are contained, whereas the purified one appear internet formed by many pieces. The grafting ratio of PSt on nmSiO2 surface could be influenced obviously by dispersion agents content and agitation speed, whereas the morphology of nmSiO2-g-PSt purified is rarely changed.
结果表明:常温下在无水乙醇介质中处理48h以上可获得良好的硅烷处理效果;PSt已接枝包覆于nmSiO2表面,接枝率约40%;经甲苯洗涤前nmSiO2-g-PSt呈多粒子包覆微球状,洗涤后呈单粒子包覆网状结构;分散剂用量、搅拌速度改变对接枝率有影响,但对包覆形态影响不大。
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The right of citizens and collectives to lawfully contract for the management of forests, mountains, grasslands, unreclaimed land, beaches and water surfaces that are owned by collectives or owned by the state but used by collectives shall be protected by law.
公民、集体依法对集体所有的或者国家所有由集体使用森林、山岭、草原、荒地、滩涂、水面的承包经营权,受法律保护。
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At present,the rate laws and reaction mechanisms of pyrite oxidation,pyrite and chalcopyrite formation,uraninite and magnetite dissolution in aqueous solutions are mainly studied,and it is discovered that (1)oxidation rate of pyrite controlled by surface reaction assume fractional dependence on Fe3+ and O2 molalities in acid solutions;(2)Pyrite cannot nucleate directly from solutions below 300 ℃,it can only form initially through sulfidation of a FeS precursor. The rate equation of reaction between FeS and H2S to form pyrite is of the second order;(3)Chalcopyrite is formed by reactions of pyrrhotite or pyrite with Cu2+,the former proceeds via a series of mediate metastable phases of Cu-Fe sulfide,whereas the rate equation of the latter is apparently of the first order which is controlled by surface reaction;(4)The nonlinear behavior of magnetite dissolution at acid pH can be represented by a shrinking core model coupling with surface reaction and diffusion transport.
目前主要研究了水溶液中黄铁矿氧化、黄铁矿和黄铜矿形成、晶质铀矿和磁铁矿溶解的速率定律和反应机理,发现:(1)酸性溶液中黄铁矿的氧化速率对Fe3+和O2浓度呈分数依赖并受表面反应的控制;(2)低于300 ℃时黄铁矿不能从溶液中直接成核,而需初始地通过FeS先驱物的硫化生成,FeS与H2S反应形成黄铁矿的速率方程为二级;(3)磁黄铁矿或黄铁矿与Cu2+反应均可形成黄铜矿,前者经历了一系列准稳的Cu-Fe硫化物的中间物,后者的速率方程为表观一级并受表面反应的控制;(4)酸性pH时磁铁矿的非线性溶解行为可采用表面反应扩散输运耦合的收缩核模型来描述。
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A new method for indexing large amounts of points inhigh-dimensional space is proposed.The basic principle is as follows:uniformly partition the data space by Lattice vector quantization,store the lattice points by Inverted File,organize the inverted file by Trie tree,and fast access the inverted file by Trie parallel search algorithm.
本文提出一种新的高维空间中点数据的索引方法,其基本原理是用格矢量量化均匀划分数据空间、用倒排文件存储格点、用Trie树实现倒排文件的组织和存储、用Trie并行搜索算法实现倒排文件的快速访问。
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Considering the specialty of pseudo-random vector, a partial scan algorithm for BIST, which unifying the structure analysis and testability analysis, is presented in this paper. Then, the fault simulation experiments on ISCAS89 benchmark circuits by this algorithm is made, and the results, which made by the full scan algorithm and the partial scan algorithm only based on the structure analysis, is compared. The conclusion is achieved: for pseudo-random vector, compared with the full scan circuit and the circuit geted by partial scan algorithm only based on the structure analysis, the circuit geted by our partial scan algorithm can greatly reduce the test time with very close or even greater faults coverage and less area than the full scan.
考虑到伪随机测试向量的特殊性,本文借鉴确定性测试向量生成的部分扫描算法,在内建自测试环境中,提出一种综合结构分析和可测性分析的部分扫描算法,PSBAST,并运用此算法对ISCAS89 benchmark电路进行计算,并对经计算后得到的部分扫描电路进行故障模拟,最后将模拟结果与全扫描电路和一种仅考虑结构因素的部分扫描算法得到的结果进行详细分析和比较,结果表明:对于伪随机测试向量,相对于全扫描电路和由仅考虑结构因素的部分扫描算法得到的电路,本文提出的部分扫描算法得到的电路,能够在不低于全扫描结构故障覆盖率前提下,大幅度减少测试时间,并能降低测试面积开销。
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Then follow the saints: Peter, prince of the Apostles, vice-regent of Christ; Paul, the Apostle of the Gentiles; Andrew, who first heeded the call of the Master; James the Greater and John the Evangelist, the beloved disciple, who, with St. Peter, were most favoured by Christ; Thomas, called Didymus, who received from Christ signal proofs of His Resurrection; James the Less, first Bishop of Jerusalem; Philip; Bartholomew; Matthew, once called Levi, the toll-gatherer, who wrote the First Gospel; Simon the Zealot; Jude Thaddeus; Matthias, who was chosen to fill the place of Judas Iscariot; Barnabas, called to the Apostolate by the Holy Ghost (Acts 13:2); Luke, the physician, writer of the Third Gospel and the Acts; Mark, the Evangelist, disciple of St. Peter; all the Apostles and Evangelists; the holy disciples of the Lord; the Holy Innocents, the infant martyr-flowers,"Who, slain at the command of Herod, confessed the name of the Lord not by speaking but by dying" Rom. Brev.
然后按照圣人:黄匡源,王子使徒,副丽晶基督;保罗,使徒的外邦人;黄宏发,谁第一个响应号召的主人;詹姆斯更大和约翰福音,心爱的弟子,谁,与圣彼得大教堂,最喜欢的基督;托马斯,所谓didymus ,谁收到了基督的信号证明他的复活;詹姆斯少,首先主教在耶路撒冷;弘;巴尔多禄茂;马修,一旦所谓的Levi ,不收费的采集,谁写的第一福音;西蒙该zealot ;裘德项;马提亚,谁被选为以填补地方犹大iscariot ;巴拿巴,所谓向使徒由圣灵(使徒13时02分);卢克,医生,作家对第三个福音和行为;马克,传道,门徒圣彼得;所有使徒和福音;圣地的门徒主;圣地无辜的,婴儿的烈士-鲜花,"谁,杀害在指挥希律,坦白的名称,主没有发言,而是由死"。
- 相关中文对照歌词
- In The Sweet By And By
- By Heart, By Soul
- By & By
- In The Sweet By And By
- By & By
- Piece By Piece, Frame By Frame
- Sweet By And By
- In The Sweet By And By
- By And By
- I Want To Be Loved By You (But Only By You)
- 推荐网络例句
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Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.
最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势,并设计了一款彩色夜视摄像机。
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Only person height weeds and the fierce looks stone idles were there.
只有半人深的荒草和龇牙咧嘴的神像。
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This dramatic range, steeper than the Himalayas, is the upturned rim of the eastern edge of Tibet, a plateau that has risen to 5 km in response to the slow but un stoppable collision of India with Asia that began about 55 million years ago and which continues unabated today.
这一引人注目的地域范围,比喜马拉雅山更加陡峭,是处于西藏东部边缘的朝上翻的边框地带。响应启始于约5500万年前的、缓慢的但却不可阻挡的印度与亚洲地壳板块碰撞,高原已上升至五千米,这种碰撞持续至今,毫无衰退。