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The terms indebtedness and obligations are （hereinafter collectively referred to as the Obligations） used herein in their most comprehensive sense and include any and all advances, debts, obligations and liabilities of Seller, heretofore, now, or hereafter made, incurred or created, whether voluntarily or involuntarily, and however arising（including, without limitation, indebtedness owing by Seller to third parties who have granted Buyer a security interest in the accounts, chattel paper and general intangibles of said third party; and further including, without limitation, any and all attorneys fees, expenses, costs, premiums, charges and interest owed by Seller to Buyer, whether under the Agreements, or otherwise） whether due or not due, absolute or contingent, liquidated or unliquidated, determined or undetermined, whether Seller may be liable individually or jointly with others, whether recovery upon such indebtedness may be or hereafter becomes barred by any statute of limitations or whether such indebtedness may be or hereafter becomes otherwise unenforceable, and includes Sellers prompt, full and faithful performance, observance and discharge of each and every term, condition, agreement, representation, warranty undertaking and pro visio n to be performed by Seller under these Agreements.

这里的术语"负债""债务"应按照各自最广泛的含义被使用，债务包括卖方先前、现在和今后，基于自愿或非自愿发生的所有预付款、负债、债务和责任，不论债务如何产生，（包括但不限于，卖方对已用自己的帐户、动产文书或一般无体物向买方提供担保利益的第三方之负债，并进而包括但不限于，卖方在协议等项下欠付买方的全部律师费、支出、费用、保险费、运费和利息），不论债务是否已经到期、是否必然发生、是否已清算、是否已决，不论卖方是单独或与他人连带承担责任，不论债务的追偿是否已经或将会受到时效法的阻碍，也不论债务是否已经或将要无法强制执行，卖方应当即时、充分和善意的履行、遵守和完成全部条款、条件、协议、陈述、担保义务和这些协议项下卖方应当履行的条款。

Including, without limitation, indebtedness owing by Seller to third parties who have granted Buyer a security interest in the accounts, chattel paper and general intangibles of said third party; and further including, without limitation, any and all attorneys fees, expenses, costs, premiums, charges and interest owed by Seller to Buyer, whether under the Agreements, or otherwise） whether due or not due, absolute or contingent, liquidated or unliquidated, determined or undetermined, whether Seller may be liable in华夏学习网idually or jointly with others, whether recovery upon such indebtedness may be or hereafter becomes barred by any statute of limitations or whether such indebtedness may be or hereafter becomes otherwise unenforceable, and includes Sellers prompt, full and faithful performance, observance and discharge of each and every term, condition, agreement, representation, warranty undertaking and provision to be performed by Seller under these Agreements.

这里的术语"负债""债务"应按照各自最广泛的含义被使用，债务包括卖方先前、现在和今后，基于自愿或非自愿发生的所有预付款、负债、债务和责任，不论债务如何产生，（包括但不限于，卖方对已用自己的帐户、动产文书或一般无体物向买方提供担保利益的第三方之负债，并进而包括但不限于，卖方在协议等项下欠付买方的全部律师费、支出、费用、保险费、运费和利息），不论债务是否已经到期、是否必然发生、是否已清算、是否已决，不论卖方是单独或与他人连带承担责任，不论债务的追偿是否已经或将会受到时效法的阻碍，也不论债务是否已经或将要无法强制执行，卖方应当即时、充分和善意的履行、遵守和完成全部条款、条件、协议、陈述、担保义务和这些协议项下卖方应当履行的条款。

Including, without limitation, indebtedness owing by Seller to third parties who have granted Buyer a security interest in the accounts, chattel paper and general intangibles of said third party; and further including, without limitation, any and all attorneys fees, expenses, costs, premiums, charges and interest owed by Seller to Buyer, whether under the Agreements, or otherwise） whether due or not due, absolute or contingent, liquidated or unliquidated, determined or undetermined, whether Seller may be liable individually or jointly with others, whether recovery upon such indebtedness may be or hereafter becomes barred by any statute of limitations or whether such indebtedness may be or hereafter becomes otherwise unenforceable, and includes Sellers prompt, full and faithful performance, observance and discharge of each and every term, condition, agreement, representation, warranty undertaking and pro visio n to be performed by Seller under these Agreements.

这里的术语"负债""债务"应按照各自最广泛的含义被使用，债务包括卖方先前、现在和今后，基于自愿或非自愿发生的所有预付款、负债、债务和责任，不论债务如何产生，（包括但不限于，卖方对已用自己的帐户、动产文书或一般无体物向买方提供担保利益的第三方之负债，并进而包括但不限于，卖方在协议等项下欠付买方的全部律师费、支出、费用、保险费、运费和利息），不论债务是否已经到期、是否必然发生、是否已清算、是否已决，不论卖方是单独或与他人连带承担责任，不论债务的追偿是否已经或将会受到时效法的阻碍，也不论债务是否已经或将要无法强制执行，卖方应当即时、充分和善意的履行、遵守和完成全部条款、条件、协议、陈述、担保义务和这些协议项下卖方应当履行的条款。

Including, without limitation, indebtedness owing by Seller to third parties who have granted Buyer a security interest in the accounts, chattel paper and general intangibles of said third party; and further including, without limitation, any and all attorneys fees, expenses, costs, premiums, charges and interest owed by Seller to Buyer, whether under the Agreements, or otherwise） whether due or not due, absolute or contingent, liquidated or unliquidated, determined or undetermined, whether Seller may be liable individually or jointly with others, whether recovery upon such indebtedness may be or hereafter becomes barred by any statute of limitations or whether such indebtedness may be or hereafter becomes otherwise unenforceable, and includes Sellers prompt, full and faithful performance, observance and discharge of each and every term, condition, agreement, representation, warranty undertaking and provision to be performed by Seller under these Agreements.

这里的术语"负债""债务"应按照各自最广泛的含义被使用，债务包括卖方先前、现在和今后，基于自愿或非自愿发生的所有预付款、负债、债务和责任，不论债务如何产生，（包括但不限于，卖方对已用自己的帐户、动产文书或一般无体物向买方提供担保利益的第三方之负债，并进而包括但不限于，卖方在协议等项下欠付买方的全部律师费、支出、费用、保险费、运费和利息），不论债务是否已经到期、是否必然发生、是否已清算、是否已决，不论卖方是单独或与他人连带承担责任，不论债务的追偿是否已经或将会受到时效法的阻碍，也不论债务是否已经或将要无法强制执行，卖方应当即时、充分和善意的履行、遵守和完成全部条款、条件、协议、陈述、担保义务和这些协议项下卖方应当履行的条款。

The terms indebtedness and obligations are （hereinafter collectively referred to as the Obligations） used herein in their most comprehensive sense and include any and all advances, debts, obligations and liabilities of Seller, heretofore, now, or hereafter made, incurred or created, whether voluntarily or involuntarily, and however arising（including, without limitation, indebtedness owing by Seller to third parties who have granted Buyer a security interest in the accounts, chattel paper and general intangibles of said third party; and further including, without limitation, any and all attorneys fees, expenses, costs, premiums, charges and interest owed by Seller to Buyer, whether under the Agreements, or otherwise） whether due or not due, absolute or contingent, liquidated or unliquidated, determined or undetermined, whether Seller may be liable individually or jointly with others, whether recovery upon such indebtedness may be or hereafter becomes barred by any statute of limitations or whether such indebtedness may be or hereafter becomes otherwise unenforceable, and includes Sellers prompt, full and faithful performance, observance and discharge of each and every term, condition, agreement, representation, warranty undertaking and provision to be performed by Seller under these Agreements.

这里的术语"负债""债务"应按照各自最广泛的含义被使用，债务包括卖方先前、现在和今后，基于自愿或非自愿发生的所有预付款、负债、债务和责任，不论债务乍样产生，（包括但不限于，卖方对已用自己的帐户、动产文书或一般无体物向买方提供担保利益的第三方之负债，并进而包括但不限于，卖方在协议等项下欠付买方的全部律师费、支出、费用、保险费、运费和利息），不论债务是否已经到期、是否必然发生、是否已清算、是否已决，不论卖方是单独或与他人连带承担责任，不论债务的追偿是否已经或将会受到时效法的阻碍，也不论债务是否已经或将要无法强制执行，卖方应当即时、充分和善意的履行、遵守和完成全部条款、条件、协议、陈述、担保义务和这些协议项下卖方应当履行的条款。

The terms indebtedne and obligatio are （hereinafter collectively referred to as the Obligatio ） used herein in their most comprehe ive se e and include any and all advances, debts, obligatio and liabilities of Seller, heretofore, now, or hereafter made, incurred or created, whether voluntarily or involuntarily, and however arising（including, without limitation, indebtedne owing by Seller to third parties who have granted Buyer a security interest in the accounts, chattel paper and general intangibles of said third party; and further including, without limitation, any and all attorneys fees, expe es, costs, premiums, charges and interest owed by Seller to Buyer, whether under the Agreements, or otherwise） whether due or not due, a olute or contingent, liquidated or unliquidated, determined or undetermined, whether Seller may be liable individually or jointly with others, whether recovery upon such indebtedne may be or hereafter becomes barred by any statute of limitatio or whether such indebtedne may be or hereafter becomes otherwise unenforceable, and includes Sellers prompt, full and faithful performance, o ervance and discharge of each and every term, condition, agreement, representation, warranty undertaking and provision to be performed by Seller under these Agreements.

这里的术语"负债""债务"应按照各自最广泛的含义被使用，债务包括卖方先前、现在和今后，基于自愿或非自愿发生的所有预付款、负债、债务和责任，不论债务如何产生，（包括但不限于，卖方对已用自己的帐户、动产文书或一般无体物向买方提供担保利益的第三方之负债，并进而包括但不限于，卖方在协议等项下欠付买方的全部律师费、支出、费用、保险费、运费和利息），不论债务是否已经到期、是否必然发生、是否已清算、是否已决，不论卖方是单独或与他人连带承担责任，不论债务的追偿是否已经或将会受到时效法的阻碍，也不论债务是否已经或将要无法强制执行，卖方应当即时、充分和善意的履行、遵守和完成全部条款、条件、协议、陈述、担保义务和这些协议项下卖方应当履行的条款。

The terms indebtedness and obligations are （hereinafter collectively referred to as the Obligations） used herein in their most comprehensive sense and include any and all advances, debts, obligations and liabilities of Seller, heretofore, now, or hereafter made, incurred or created, whether voluntarily or involuntarily, and however arising（including, without limitation, indebtedness owing by Seller to third parties who have granted Buyer a security interest in the accounts, chattel paper and general intangibles of said third party; and further including, without limitation, any and all attorneys fees, expenses, costs, premiums, charges and interest owed by Seller to Buyer, whether under the Agreements, or otherwise） whether due or not due, absolute or contingent, liquidated or unliquidated, determined or undetermined, whether Seller may be liable in华夏学习网idually or jointly with others, whether recovery upon such indebtedness may be or hereafter becomes barred by any statute of limitations or whether such indebtedness may be or hereafter becomes otherwise unenforceable, and includes Sellers prompt, full and faithful performance, observance and discharge of each and every term, condition, agreement, representation, warranty undertaking and provision to be performed by Seller under these Agreements.

这里的术语"负债""债务"应按照各自最广泛的含义被使用，债务包括卖方先前、现在和今后，基于自愿或非自愿发生的所有预付款、负债、债务和责任，不论债务如何产生，（包括但不限于，卖方对已用自己的帐户、动产文书或一般无体物向买方提供担保利益的第三方之负债，并进而包括但不限于，卖方在协议等项下欠付买方的全部律师费、支出、费用、保险费、运费和利息），不论债务是否已经到期、是否必然发生、是否已清算、是否已决，不论卖方是单独或与他人连带承担责任，不论债务的追偿是否已经或将会受到时效法的阻碍，也不论债务是否已经或将要无法强制执行，卖方应当即时、充分和善意的履行、遵守和完成全部条款、条件、协议、陈述、担保义务和这些协议项下卖方应当履行的条款。

The unoperated sides of the treated animals also served as controls. Six normal rats were treated as normal control group. Three different siRNA plasmid solution containing RC2-Ⅰ, MAFbx-Ⅱ, CON (50μl , 0.8μg/μl)was injected and transfected by electroporation as methods mentioned above, respectively. The changes of RC2 and MAFbx mRNA levels and RC2 protein levels after 3 days were determined by real-time quantitative PCR and Western blot, respectively. On postoperative 2, 3 and 4 weeks, the rate of wet muscle weight preservation, mean diameter of muscle fiber and mean cross-section area of muscle fiber and muscle protein content were checked and then compared between group CON and group RC2 or group MAFbx, respectively. The differences between groups were analyzed by one-way ANOVA. Ultrastructural changes of muscle fiber were observed at 2, 3, 4 weeks postoperation.Results GFP plasmid was efficiently deliverd into muscle by electroporation and robust GFP expression in muscle could be observed more than three weeks. Histology shows that injected plasmid DNA diffuses extensively in muscle tissue.

1、健康雌性SD大鼠18只，随机分为电穿孔组和非电穿孔组，每组9只，制作右下肢趾长伸肌失神经支配模型；EP组为将质粒pEGFP-N1溶液50μl(0.8μg／μl)注射入右趾长伸肌后，立即于两侧腱腹交接处给予电穿孔，电穿孔参数为：电场强度为200V／Cm，脉冲100μs，频率1Hz，施加10次脉冲；NEP组仅质粒pEGFP-N1溶液注射；转染后1、2、3周，荧光显微镜下观察趾长伸肌中GFP的表达情况，转染后1周行Western印迹检测趾长伸肌中GFP蛋白的表达情况，检测和优化体内转染效率。2、健康雌性SD大鼠78只，随机分为失神经对照组、RC2基因治疗组(RC2组)，MAFbx基因治疗组，每组24只，制作右下肢趾长伸肌失神经支配模型，余6只为正常组；分别将含CON、RC2、MAFbx基因的siRNA重组质粒注射入趾长伸肌，之后给予电穿孔，方法同上；治疗后3天实时定量PCR和Western印迹检测各组中RC2或MAFbx基因的mRNA和蛋白的表达变化，治疗后2、3、4周检测各组肌湿重维持率、肌细胞直径和肌细胞截面积，肌细胞超微结构变化以及肌纤维中蛋白含量变化。

His characters are not modified by the customs of particular places, unpractised by the rest of the world; by the peculiarities of studies or professions, which can operate but upon small numbers; or by the accidents of transient fashions or temporary opinions: they are the genuine progeny of common humanity, such as the world will always supply, and observation will always find.

他的人物塑造并不拘泥于只为一部分人所遵循的某个特定地区的习俗，也不局限于一小部分人所从事的特定的研究或职业，也不追随短暂的潮流或暂时的思想观点：他们据有人们一贯具备的、普遍的人性特点。就像世界能永不竭地供应，眼睛能永不停地发现。

His characters are not modified by the customs of particular places, unpractised by the rest of the world; by the peculiarities of studies or professions, which can operate but upon small numbers; or by the accidents of transient fashions or temporary opinions: they are the genuine progeny of common humanity, such as the world will always supply, and observation will always find .

他是一位自然的诗人，他的作品将人间百态真实地展现在读者眼前。他的人物塑造并不拘泥于只为一部分人所遵循的某个特定地区的习俗，也不局限于一小部分人所从事的特定的研究或职业，也不追随短暂的潮流或暂时的思想观点：他们据有人们一贯具备的、普遍的人性特点。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。