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Methods: Human leukemia cell line K562 was treated with different concentrations of WM. The proliferation of K562 cells was examined by MTT assay. DNA damage in K562 cells was examined by single cell gel electrophoresis assay, and apoptosis of K562 cells was detected by Annexin V-FITC/PI double staining. The expressions of total Akt, phosphorate Akt, and NF-κB p65 mRNA and protein were detected by RT-PCR and Western blotting, respectively.

以不同浓度的渥曼青霉素作用于人类髓细胞白血病细胞K562，采用MTT法检测细胞增殖活性，单细胞凝胶电泳技术检测细胞DNA损伤形成的"彗星"样拖尾现象，Annexin V FITC/PI双标法检测细胞凋亡，Western blotting、RT-PCR检测渥曼青霉素作用K562细胞后总Akt和磷酸化Akt以及NF-κB基因及蛋白表达水平的变化。

The test was divided into three groups randomly as yh-16 - experiment group, pbs- blank control group and rpmi-1640 medium - positive group.③in the following 24h、48h and 72h interventions, the morphous of ec was observed by photology inverted microscope, the cytoactive was detected by method of mtt and the cell cycle was investigated by flow cytometry.the expression of vegf was dectected by real-time fluorescent quantitate polymerase chain reaction(real-time pcr).

以有效浓度的yh-16作用于ec，在不同时相点光镜下观察ec形态，mtt检测细胞活性，流式细胞术检测细胞周期，实时荧光定量pcr检测vegf的表达量。

Results: Among 32 tested MNs, depolarizing responses(excitatory postsynaptic potential, EPSP) were elicited by cVLF stimulation in 21 MNs, hyperpolarizing response (inhibitory postsynaptic potential, IPSP) in 1 MN, and IPSP preceded by EPSP in 4 MNs. The cVLFEPSPs were stimulus intensity-dependent, and could be abolished by low Ca(superscript 2+)/high Mg(superscript 2+) solution. compared with iVLF-EPSPs, cVLF-EPSPs showed longer latency (P.001). The cVLF-IPSP presented with membrane potential-dependent property and was eliminated by the perfusion of picrotoxin (30 μmol/L) and strychnine (1.0 μmol/L).

结果：在32个测试的MNs，观察到cVLF电刺激可在21个MNs上诱发去极化反应（即cVLF性兴奋性突触后电位，cVLF-EPSP），在1个MN上诱发超极化反应（即cVLF性抑制性突触后电位，cVLF-IPSP），在4个MNs上诱发cVLF-EPSP后复合有cVLF-IPSP的反应。cVLF-EPSP具有刺激强度依赖性、被低钙高镁溶液取消的特性，与i VLF性EPSP相比，有潜伏期较长的特点(P.001)。cVLF-IPSP呈膜电位依赖性，并被印防己毒素(30μmol/L)及士的宁(1.0μmol/L)取消。

A:Group1,widely opened by 20 μg·kg-1 of AD;B:Group 2,moderately opened by 10μg·kg-1 of AD;:Group 3,Slightly opened by 5μg·kg-1 of AD;D:Group 4,except a non-BBB area,pineal body,BBB was not opened in adjacent brain area by 3μg·kg-1 of AD.

a：第一组，AD 20 μg·kg-1引起的广泛开放；B：第二组，AD10 μg·kg-1引起的中度开放；C：AD 5 μg·kg-1引起的轻度开放；D：除缺乏BBB的松果体之外，AD3 μg·kg-1未引起其它脑区的BBB开放

At present, Zhao organized and the management Chinese side Enterprise has been evaluated by the Chinese five ore chemical industry import and export chamber of commerce AAA the level credit enterprise, by the Agriculture Bank of China Shanxi Province Branch, the letter bank Taiyuan Branch, the Minsheng Bank Shanxi branch is evaluated not bad record and defends the credit enterprise, is evaluated tax payment pivot man, the demonstration enterprise by Internal Revenue Service, in the customs, the outer tube aspect is prompt, the code of honor, does not have any bad record, evaluates the model enterprise by Tianjin and Dalian Customs, and after-crops "not bad records" the enterprise document; Zhao managed the foreign enterprise is evaluated the No.1 enterprise, some section of Listed enterprise produced the product was also commended US's; Zhao now in the US, Japan, Singapore, Australia, Germany and Britain has the enterprise and the staff, altogether managed 15721 professional supervisors, to buy the hand, to sell the hand and the staff member.

目前，赵尚阳创办和管理的中方企业已被中国五矿化工进出口商会评为AAA级信用企业，被中国农业银行山西省分行、中信银行太原分行、民生银行山西分行评为无不良记录和守信用企业，被国税局评为纳税标兵、示范企业，在海关、外管方面规范、守信，无任何不良记录，被天津和大连海关评为模范企业，并下发"无不良记录"企业文件；赵尚阳管理的外商企业被评为No.1企业，有一上市企业生产的产品还受到美国的肯定和表彰；赵尚阳现在在美国、日本、新加坡、澳大利亚、德国和英国都有企业和工作人员，共管理着15721名职业管理人、买手、卖手和职员。

This invention relates to television human interface generation method, which comprises the following steps: receiving user order by remoter and sending it to the television for its CPU to receive the order and to inform the OSD module to relative OSD process and display by the keys and the executing the relative operation according to the above order; the OSD module dispatching Plash memory pre-stored pictures and processing the relative OSD signals and displaying the OSD content onto screen, wherein the content comprises main menu, sub menu and relative content; during relative operation by television, OSD module dispatching multi-picture stored in the Flash memory to execute cartoon display; after the relative operations by television, the OSD module stopping the display.

要約 一种电视机人性化交互界面的产生方法，其特征在于它包括以下步骤：由遥控器接收用户指令，并将该指令发送到电视机；电视机中的CPU接收上述指令，并通知OSD模块，以便按照所按按键对应的功能执行相应的OSD处理和显示，同时电视机按照上述指令执行相应的操作；OSD模块调用Flash存储器中预先存储的图片并进行相应的OSD信号处理后，将OSD内容显示到屏幕上，所述OSD内容可同时包括主菜单、子菜单以及相应的子菜单内容；在电视机执行所述相应操作的同时，OSD模块调用Flash存储器中存储的多幅动画图片来执行动画显示；在电视机完成所述相应操作后，OSD模块停止执行所述动画显示。

objectiveto predict the promoting effect of chinese medicine on proliferation of rat bone marrow mesenchymal stem cells by bioactivity fingerprint in vitro.methodsthe ointment of plastrum testudinis was extracted by petroleum ether,ether and dichloromethane in burn to gain the extracts.the effects on the proliferation of bmmscs were examined by mtt assay and flow cytometry analysis, and each sample was studied by gc-ms.

目的运用生物活性指纹图谱预测其它中药能否促进骨髓间充质干细胞（bone marrow mesenchymal stem cells，bmmscs）。方法龟版浸膏依次用石油醚、乙醚、二氯甲烷提取，得到溶剂提取物，用mtt 比色法及流式细胞仪器研究了溶剂提取物干细胞活性，采用gc-ms 技术研究每一个提取物，根据总离子流色谱图的相同保留时间和相应峰面积，获得龟版浸膏 gc-ms 生物活性指纹图谱。

By applying these two tools,we mainly discuss the problems as follows:1 Perturbation identities for the generalized polar decomposition:By applying singular value decomposition, we study the perturbation bounds of the generalized polar decomposition under unitary invariant norms when A is perturbed by addition or multiplication,respectively and give our bounds in identities.2 A class of new unitary invariant metrics: This paper generalized a class of new unitarily invariant metrics and give the upper-bounds and lower-bounds by C-S decomposition.

利用这两个强有力的工具，本文主要讨论了如下问题：1 矩阵广义极分解的扰动等式：利用奇异值分解，本文分别讨论了加法扰动和乘法扰动下矩阵广义极分解在任意酉不变范数下的扰动界，并首次以恒等式的形式给出了酉极因子和半正定因子的扰动界。2 一类新的酉不变度量：本文推广了文[22] 中的结论，定义了一类新的酉不变度量。

The method of reading the line, arc, spine, tolerances, surface roughness and full messages of the part from DXF file generated by AutoCAD is mainly introduced. Based on the study of DXF, a program interface is developed, by which the data can be shared among different CAD systems. According to the different Characters, the respective tool paths are designed. And by a machine configure file, the General postposition process is deal with. On the research of thegeneral simulation technology that is based on the NC codes. By analyzing the characteristics of the CNC system and the NC codes, a set of general simulation interface is developed.

本文主要介绍如何从AutoCAD生成的DXF文件中读取直线、圆弧、样条曲线、尺寸公差、表面粗糙度等信息及零件的全局信息，结合回转体零件的特点，通过对应用广泛的DXF的深入分析，建立了与不同CAD系统实现数据共享的编程接口；根据零件的不同加工特征提出了相应的走刀路线，并通过机床配置文件，初步实现了通用后置处理；研究了数控系统和NC代码的特点，建立了仿真通用接口，并提出代码翻译地基本思路。

The voltage and the sensitivity of voltage to system operation parameters are calculated by Newton power flow approach; the various higher order derivatives of voltage and voltage sensitivity to line openbreak parameter can be obtained by using the convergent correction equation of power flow and the equation of calculating sensitivity for the N power system. Finally, the voltage and sensitivity of N-1 power system can he calculated by correcting the voltage and sensitivity of N power system by Taylor series expansion.

通过牛顿潮流计算出N网络的各节点电压及其对系统运行参数的灵敏度；利用已收敛的潮流修正方程式和N网络灵敏度计算方程式分别对线路开断参数多阶求导，从而计算出电压和灵敏度对此线路开断参数的多阶导数值；根据泰勒级数展开式修正N网络的电压和灵敏度值，得到N-1网络的电压和灵敏度值。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。