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Abortus, amplify BP26 gene by PCR and clone to pMD18-T simple vector. Identify the constructed recombinant plasmid pMDBP26 by sequencing, then subclone to vector pET-28a. Transform the constructed recombinant plasmid pETBP26 to E. coli BL21(DE3) for expression under induction of IPTG. Purify the expressed product by histidine-binding resin column chromatography and identify by Western blot.

提取布鲁氏菌基因组DNA，用PCR扩增出BP26基因，并克隆到pMD18-T载体上，测序正确后，再亚克隆至pET-28a载体上，转化到大肠杆菌BL21(DE3)中，用IPTG诱导表达，经组氨酸结合树脂柱纯化，并对纯化后的表达产物进行Western blot鉴定。

The plasma density scale length decided by main laser pulse in the experiment is suitable for Vacuum Heating, and it is the domiant process for hot electron generation. From the scaling law about hot electron temperature given by VH model, the hot electron measured by experiment was reasonable, the temperature was higher than the temperature given by Rcsonant Absorption scaling law.

从真空吸收给出的超热电子温度的定标率来看，实验中测量得到的超热电子温度是合理的。X射线是各向同性的，X射线产额随入射激光强度的降低而减小，当强度小于10〓W/cm〓时，不再有硬X射线产生。

Aqueous extract of cotton has allelopathic effect on Raphanus sativus, Brassica campestris, Lactuca indica, Brassica campestris, Echinochloa crusgall, Setaria viridis and Abutilon theophrasti and its inhibition effect gradually strengthens with increasing concentration. Aqueous extracts from stems, leaves and roots of cotton inhibit or accelerate seed germination and seedling growth, and activity of enzyme of seedling root of cotton. While aqueous extracts from stems and leaves of cotton are 0.02 gmL^(-1), root length, shoot length, lateral root number and dry weight of cotton respectively increase by 10.3%, 3.1%, 22.9% and 2.4% over the control. The above mentioned indexes are lower than the control by 34.4%, 39.2%, 40.6% and 19.0% respectively as the aqueous extracts are 0.1 gmL^(-1). At the same time, root activity and SOD activity decrease by 38.2% and 36.7%, while POD activity and MDA content increase by 52.3% and 36.5%. Growth indexes of plant receptors increase at low concentrations but decrease at high concentrations of aqueous extracts. The allelopathic effect of aqueous extract of stems and leaves is stronger than that of root.

结果表明：棉花植株水浸提液对萝卜、白菜、莴苣、油菜、稗草、狗尾草、苘麻均具有化感效应，随着作用浓度增加，抑制效应逐渐加强；棉花茎叶和根系水浸提液对棉花种子萌发、幼苗生长、幼苗根系活力、根系酶活性有抑制或促进作用，当茎叶浸提液浓度为0.02 gmL^(-1)时，棉花根长、苗高、侧根数、干重分别比对照增加10.3%、3.1%、22.9%和2.4%，当浓度为0.1 gmL^(-1)时，上述指标与对照相比分别降低34.4%、39.2%、40.6%和19.0%，而根系活力、SOD活性比对照降低38.2%、36.7%，POD活性、MDA含量较对照增加52.3%和36.5%，不同浓度棉花植株水浸提液对棉花生长呈现"低促高抑"的规律，且茎叶浸提液作用效应大于根系浸提液。

By synthesizing theories of systems engineering, science of talent, talent assessment, performance appraisal and bibliometrics, the author analyzes the qualification structure and performance structure of theachers in military academies and sets up a basic structure and concrete appraisal indicator system of qualifications of technical specialty for teachers in colleges. The author puts forward new appraisal indicators such as "lecuring hours coefficient","republication coefficient","thesis of core journals coefficient" and "citation coefficient". Comparatively complete quantification approaches are invented by the author for working performance of teaching, researching and social service of teachers. Based on this, the author establishes an integrated method to generate the appraisal results. By the application of stadardized processing on the appraisal results and synthetization of weighted indexes, the author solves the addibility and comparability problems of differentiated appraisal results by category or dimensiok, and realizes the amalgamation and comprehensive appraisal of inflexible appraisalresults and flexible appraisal results, and probes into the testification methods of validity, consistency and sensitivity analysis for the indicators system.

综合运用系统工程理论、人才学理论、人才素质测评理论、工作绩效评估理论、文献计量学等原理与方法，分析研究了军校教师的素质结构和绩效结构；建立了高校教师专业技术资格评审条件基本结构和具体评价指标体系，提出了"完成主讲课程课时系数"、"著作重版系数"、"核心期刊论文系数"、"论文被检索引用系数"等若干新的评价指标；提出了比较完整的教师的教学、科研、社会服务等工作业绩的量化方法；以此为基础，建立了评价结果的综合方法，通过对评价结果的标准化处理，运用指标加权综合法，解决了不同类别、不同量纲指标的评价结果的可加性和可比较性问题，实现了硬评价结果和软评价结果的融合和综合评价；探讨了指标体系有效性、一致性、灵敏度分析的检验方法。

In 《Hero's Return》, the astronaut protected by the guards is received by a welcoming crowd. In the first painting, he appears rigid, uneasy and overwhelmed by the scale of the event. In fact, he looks more like a criminal being marched to the execution ground. But in the second painting, he is shown to wave to the crowd and play the role of 'hero' with adeptness. We can almost begin to see the life he leads afterwards. He will be received by various top leaders and interviewed in television programs. In a succession of awarding ceremonies, speeches and briefings, he will become more and more experienced, relaxed and eloquent, so on and so forth. His self will be eroding. Whenever he looks at himself in the mirror, he will see a hero, a hero through and through. Via the contrast of images, the artist subtly reveals the process of hero-making.

在《英雄归》里，那位宇航员在卫兵的护送下，接受人群的夹道欢迎，第一幅画面上，我们可以看到他显得举止僵硬，受宠若惊，仿佛还没有适应如此盛大的场面，夸张地说，他的姿态倒像是一个被押送的犯人，而在第二幅画面上，他已经在挥手致意，看上去已经进入到"英雄"这一角色之中，我们似乎可以猜想他此后的生活，他将会不停地受到领导人的接见，不停地出席电视采访节目、受勋仪式、演讲会、报告会，在那样的过程中越来越老练、坦然，口才越来越出色，如此等等，他的自我逐渐地丧失，甚至当他揽镜自照时，眼之所见也俨然是一个不折不扣的英雄形象。

At baseline and on day 1, 3, 7, 14, 21 following MPP+ injection, the degree of microglial activation was examined by detecting the immunodensity of OX-42 in the substantia nigra. The number of viable dopaminergic neurons was determined by measuring tyrosine hydroxylase positive neurons in the SN. Behavioral performances were evaluated by counting the number of rotations induced by apomorphine, calculating scores of forelimb akinesia and vibrissae-elicited forelimb placing asymmetry.

分别在基线、MPP+注射后第1、3、7、14、21天时通过测定黑质区域OX-42的免疫荧光强度评定小胶质细胞的激活程度，测定酪氨酸羟化酶的表达情况评定存活多巴胺神经元的数量；通过阿朴吗啡诱导的旋转行为、前肢跨步及触须引发的不对称放置试验得分测评行为学表现。

In settling an island, the first building erected by a Spaniard will be a church; by a Frenchman, a fort; by a Dutchman, a warehouse; and by an Englishman, an alehouse.

在海岛定居时，西班牙人先造教堂；法国人先造要塞；荷兰人先造仓库；而英国人则先造酒店。

In settling on land, the first building erected by a Spaniard will be a church; by a Frenchman, a fort; by a Dutchman, a warehouse; and by an Englishman, an alehouse.

""定居一地后，西班牙人首先会建造一座教堂，法国人会建一个港口，荷兰人会建一间陶器作坊，英国人会建一个酒店。他们在家也喝酒，很自然地，喜欢喝酒的人们造出了许多与酒有关的谚语，试举几例

In settling on island,the first building erected by a Spaniard wil be a church; by a Frenchman,a fort; by a Dutchman,a warehouse; and by an Englishman, an alehouse.

在海岛定居时，西班牙人先造教堂；法国人先造要塞；荷兰人先造仓库而英国人则先造酒店。

The antibodies in plasma were also detected by indirect antiglobulin test. The result showed that levels of WBC,TB,DB,Glu and reticulocyte in six patients treated with β-lactam antibiotics were raised remarkably. DAT was strongly positive,but no RBC antibodies were detected in the plasma by IAT. The binding of red cells by monocytes or lymphocytes was great,and the hemolysis of red cells by alexin not appeared. After stopping above-mentioned β-lactam antibiotic administration,patient's WBC,TB,DB,and Glu levels returned to normal values.

结果发现：6例患者的临床治疗的药物都属于β内酰胺类；住院期间患者的WBC、TB、DB、Glu的检测结果出现异常，红细胞的DAT试验结果均为阳性，IAT试验结果均为阴性；将DAT阳性的红细胞进行补体结合实验时，结果为阴性；细胞涂片见部分红细胞表面有颗粒状物质附着，细胞培养见部分红细胞被白细胞识别、黏附；改用其它抗生素后，患者的上述化验指标恢复到正常值范围内，DAT试验结果转为阴性。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新