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The effect of genistein (0.8 mg/kg) was partially inhibited by L-NAME, or by sodium orthovanadate (50 μg/kg), a potent inhibitor of protein tyrosine phosphatase;(2) Genistein also decreased the PP of renal vascular bed in a dose-dependent manner. The effect of genistein was completely inhibited by pretreatment with sodium orthovanadate, but unaffected by L-NAME;(3) Genistein decreased the PP of mesenteric vascular bed in a dose-dependent manner, an effect which was partially inhibited by sodium orthovanadate, but unaffected by L-NAME.

GST的这一效应可被L-硝基精氨酸甲酯部分阻断，预先注射蛋白酪氨酸磷酸酶抑制剂正钒酸钠(50 μg/kg)，可部分抑制GST (0.8 mg/kg)引起的效应；(2)向肾血管灌注环路中直接注射 GST 也可剂量依赖性地降低肾动脉的灌流压，预先注射正钒酸钠可完全抑制GST引起的效应，而L-NAME对此效应没有影响；(3)肠系膜血管灌流环路中注射GST可剂量依赖性地降低其灌流压，这一效应可被正钒酸钠部分抑制，而L-NAME对此无影响。

Results: six patients with vagal reflection were cured by intravenous atropine, 2 with pericardial hemorrhage were treated by pericardiocentesis, 1 patient developed hypotension due to occlusion of the mitral valve orifice by the catheter was corrected by shortening the duration of the catheter in left ventricle, the blood pressure of 1 patient with rapid atrial flutter was elevated by intravenous cedilanid, the blood pressure of 1 patient with hypotension of unknown origin was elevated by vasopressor and atropine.

结果：其中迷走神经反射6例，经静脉注射阿托品纠正；心包出血2例，心包穿刺抽液血压至正常；球囊导管阻塞二尖瓣口1例，缩短导管停留在左心室时间，可以避免低血压反应；快速心房颤动1例，使用西地兰减慢心室率，血压回升；原因不明1例，经使用升压药及阿托品后血压改善。

2 Any notice required or permitted hereunder shall be in writing and shall be deemed given as of the date it is: delivered by hand; by overnight or next day delivery; received by Registered or Certified Mail, postage prepaid, return receipt requested; or received by facsimile, as can be presumptively demonstrated by return fax or letter demonstrating successful facsimile transmission; and addressed to the party to receive such notice at the address and/or facsimile telephone number set forth below, or such other address as is subsequently specified to the notifying party by the receiving party in writing.

11.2 本协议项下所有通知应当以书面形式为之，并且在下列情况下视为已经交付：专人递送；经由隔日送达业务递送；由挂号邮件或保证邮件方式接受，邮资预付，需要回执；由传真接收，并回传真或信函证明已经成功发送；通知应当按照下列地址送交当事人，或按照下列电话号码传送，或者按照接受一方最近书面通知的地址或号码为准

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

The method according to claim 6, for manufacturing a blade wherein a part external to the shell comprises a blade rooting part having at least one attachment bar, in which an end portion for linkage to a hub of said rotor is fitted with a laminate attachment, also comprising thermoplastic composite, and extending said at least one spar bar housed in the shell, the method further comprising the step of producing the elementary piece comprising the at least one spar by producing each attachment bar and the spar bar in the form of a strip of continuous and unidirectional reinforcing fibres which are agglomerated by said thermoplastic matrix, obtained by pultrusion at a melting temperature of the matrix, of several plies of continuous and unidirectional fibres which are agglomerated by said matrix, and, if said rooting part comprises at least two attachment bars, each one of which respectively extends one spar bar, in superimposing parts of corresponding strips forming the spar part housed in the shell, with interposition of at least one ply of continuous and unidirectional fibres which are agglomerated by said matrix between the adjacent superimposed strip parts, and fitting a removable tool, in the shape of a wedge, between parts held spaced from each other of two neighbouring strips, to form two attachment bars of the rooting part, then arranging the strips in a mould, and inserting each linkage end portion of a pultruded strip between two laminate elements of the attachment, to superimpose all the laminate elements of the attachment, closing the mould and compacting its contents under pressure and at a melting temperature of said thermoplastic matrix, and solidifying the matrix by cooling to rigidify the spar thus obtained with the laminate attachment solidly attached to its rooting part.

该方法根据索赔六，制造业刀片其中的一部分，罐壳外部包括一个叶片生根的一部分，拥有至少一个实习酒吧，在其中一结束部分为联系枢纽的说，转子是带有附件的层压板，也包括热塑性复合材料，并延长说，至少有一石酒吧住在蚬壳公司，进一步组成的方法步骤，生产初级一块组成的至少有一个SPAR公司生产每个附件的酒吧和酒吧在SPAR公司的形式，带不断加强和单向纤维是由烧结说，热塑性矩阵，得到的拉挤在熔化温度矩阵，数层的连续和单向纤维是由烧结说，矩阵，如果说，生根部分组成，至少有两个实习酒吧，每个人都分别延长一石酒吧，在叠加部分，相应的带形成的SPAR公司的一部分，居住在蚬壳公司，与干预的至少有一单层连续和单向纤维是由烧结说，矩阵之间的毗邻叠加条形零件，及装修一个可移动的工具，在形状，离间，部分之间举行的间隔从对方的两个邻国带，形成两个附件酒吧的生根部分，然后安排带在一个模具，并插入每个联系年底部分一拉挤带之间的二板的要素扣押，叠加所有层压板的要素扣押，关闭模具和压缩其内容的压力下和在熔化温度说，热塑性矩阵，并巩固矩阵冷却 rigidify该SPAR公司，从而获得与层压板的附着牢固重视其生根的一部分。

The mian works are as follows:(1)By coprecipitation technique and coprecipitation- gel technique the nano zirconia powder was produced, factors affecting the properties of nano zirconia powder such as pH and the content of stabilizator are analyzed;(2)By the new pressless sintering process, the nano zirconia ceramic was produced, factors which can affecting the properties of nano zirconia sinter body such as moulding press and the sinter temperature are analyzed;(3)By the microtherm pressless sintering process, the nano copper added functional zirconia ceramic composite was produced, microscopic dimensionality and electric properties of such sinter body and the factors affecting such sinter body are analyzed;(4)By the self-invented "external albumen coating" technique the zirconia powder was successfully coated by carbon in the corresponding sinter body, which can help solve the traditional aggregation problem to some degree;(5)Based on analyze the characteristics in the nano powder sinter process, the "three-ball sinter model" was proposed, for the relationship between the relative density and porosity of sinter body, by mathematical fitting, such model was successfully verified.

本研究比较系统地研究了纳米氧化锆陶瓷的合成与成型机理，特别是讨论了纳米氧化锆陶瓷的稳定性，为防止纳米颗粒在外场作用下的团聚和长大提供了理论基础和实验数据，具体工作有以下几方面：(1)采用共沉淀法和共沉淀-凝胶法制备了纳米氧化锆粉体，并分析了pH值和稳定剂含量对粉体性能的影响；(2)采用新型无压烧结工艺制备了纳米氧化锆陶瓷，并分析了成型压力和烧结温度对烧结体性能的影响；(3)采用低温无压烧结工艺制备出添加纳米铜的功能性纳米氧化锆复合陶瓷，并分析了烧结体的微观尺度和电学性能的变化以及影响因素；(4)通过自创的&鸡蛋清外敷法&在真空镀膜台上对纳米氧化锆粉体进行包敷碳颗粒的表面处理，并采用真空烧结工艺制得相应烧结体，初步开始解决陶瓷烧结过程中团聚长大这一传统难题；(5)通过分析纳米颗粒在制备烧结体过程中的实际特点，提出了&三球烧结数学模型&，通过引入坯体相对密度和孔隙率的相对关系分析并通过自行编写的数学拟合软件，验证了这一数学模型。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

The sizes of the self-assembly micelles of the amphiphilic block copolymers and the effects werestudied by dynamic light scattering and UV-visible spectrophotometer. The micelle sizes werelargely influenced bythe hydrophobic chain content in the copolymer, the properties of the solvent and the copolymer concentration in the organic phase. The morphology of the micelles was investigated by transmission electron microscopy. Theresults showed that the micelles appeared spheres with inner core and outer shell. The critical association concentrations were determined by pyrene monomer fluorescence probe technology. The degradability of copolymer was studied by the loss of the intrinsic viscosity in the degradation process. The results indicated that the degradation rate of PECL was slower thanthat of PELLA and PEDLLA. The stability of the copolymer micelle dispersion was examined by measuring the critical flocculation concentration, which gradually decreased with increasing the content of thehydrophobic chain in the copolymer. The rheological results showed thatthe viscosity ofthe micelle dispersion with higher content of PEG segments was firstly decreased and then increased with the increasingthe temperature. Otherwise, the variation of viscosity was irregular. The viscosity of the micelle dispersion increased with the increase of the electrolyte (Na 2SO 4) concentrations. Polymer micelles paclitaxel was prepared by self-emulsification/solvent evaporation method and solid dispersion technique with amphiphilic block polymers as the carrier material.

运用动态光散射和紫外分光光度计研究了两亲性嵌段共聚物自组装胶束的粒径及其影响因素，结果表明，自组装胶束的形成机理受制备方式的控制，胶束的粒径随着共聚物相对分子质量或疏水嵌段相对分子质量和有机相中共聚物浓度的增大而增大，随着有机溶剂的水溶性的增强而减小；采用透射电镜观察胶束的形态结构，发现胶束呈具有核壳结构的均匀球形；采用芘荧光探针法测两亲性嵌段共聚物的临界聚集浓度；通过降解过程中共聚物特性粘度的变化研究两亲性共聚物的降解性能，发现与PELLA和PEDLLA的相比，PECL 降解速率较慢；随着共聚物中疏水嵌段含量的增大，纳米分散液的稳定性逐渐下降；PEG含量高的PEDLLA纳米分散液随着温度的升高，纳米分散液的粘度先下降然后增大，反之，没有规律性，且随着电解质Na 2SO 4浓度的增大，体系粘度增大。

For preparing for the researches of text digital watermarking and advantaging other researchers" development when using the Chinese mathematical expression theory, this paper designs and realizes a development platform of Chinese mathematical expression. It has been proved by experiments that the platform"s function, execution speed and stability can satisfy the requirements of conversion and operation of enormous data.This research found out that the structure information of Chinese have plenty redundant information that can be used by the Chinese mathematical expression for the digital watermarking, the data embedded can possess better robustness. Based on this finding, this paper designs a kind of text digital watermarking method of embedding watermarking by using Chinese structure, and realizes the method by using the Chinese mathematical expression platform.Considering the watermarking embedding algorithms importance to the robustness of the text digital watermarking, this paper makes fully use of the advantage of the Chinese mathematical expression theory to design a kind of layered text method, which has being realized by using the Chinese mathematical expression platform. The layered text method can handle the text as a solid structure, and the embedding data havepreferable robustness.Finally, based on the researches of the text digital watermarking method and the text layered method, this paper integrates the method of making use of the Chinese structure embedding watermarking with the robust algorithm based on text layered, and realizes a brand-new text digital watermarking method. Proved by lots of experiments, this brand-new method has good robustness and better invisibility.

为了为研究工作做准备和方便其他使用汉字数学表达式理论体系的人进行开发，本文基于汉字数学表达式理论设计实现了一个汉字数学表达式平台，实现了Unicode汉字集中的汉字、汉字数学表达式、汉字的Unicode码之间的相互转化、依据汉字数学表达式提取汉字的结构信息、由汉字数学表达式在非中文平台上不依赖字库显示汉字的图像等功能，使用复合文档技术和流压缩技术进行数据的存储，使用对汉字数学表达式进行二叉树拆分来处理汉字数学表达式中包含的信息，使用自定义哈希函数来提高运行速度，实验证明，该平台功能、速度和稳定性完全能满足大量数据的转化和运算要求；通过研究表明汉字的结构信息中存在大量可使用汉字数学表达式理论加以利用的水印加载空间，加载在该空间的数据可以具有较好的鲁棒性，基于此，本文设计了一种利用汉字结构加载水印的文本数字水印方法，并使用汉字数学表达式平台将其实现；由于水印嵌入算法对于文本数字水印的鲁棒性至关重要，本文充分利用汉字数学表达式理论的优点，设计了一种文本分层的方法，并使用汉字数学表达式平台将其实现，使文本成为一个立体的空间，使嵌入在其中的数据具有更好的鲁棒性；最后在基于汉字数学表达式的文本数字水印嵌入方法的研究和基于汉字数学表达式的文本分层方法的研究的基础上，结合利用汉字结构嵌入水印的方法和基于文本分层的抗攻击文本水印嵌入算法实现了一种全新的文本数字水印方法，经实验证明，该方法有良好的鲁棒性和较好的隐蔽性。

A it is predominantly derived from the initial variety or from a variety which itself was predominantly derived from the initial variety, while retaining the expression of essential characteristics that result from the genotype or combination of genotypes of the initial variety; an essentially derived variety may have been obtained by the selection of a natural or induced mutant or of a somaclonal variant, by the selection of a variant individual from plants of the initial variety, by backcrossing, by a transformation by genetic engineering, or by any other similar method

一个品种主要由原始品种演化而来，或主要由原始品种的实质性派生品种演化而来，同时保留了来自原始品种基因型或基因型组合的基本特征的表达；一个实质性派生品种可能通过选择自然变异或诱变或体细胞无性变异、原始品种植株的个体变异的选择、回交、基因工程的改造或任何其它类似方法而获得。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。