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In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

After three years working experience, we has achieved many design projects successfully includes, the second stage landscape engineering design of Huankou Garden developed by Wuhan Real Estate Investment Group, the planning design and architecture design of Zonglian International Residential District developed by Wuhan Zonglian investment Co., Ltd, Phoenix Town developed by Wuhan Huayi Real Estate Co., ltd, Chutian Thermal Spring Hotel developed by Liannin Jingjiang Tourism company, Enshi Genevese Amorous District, the landscape design for new office building of Wuhuan Tech joint-stock co., ltd, landscape design for headquarter of Zhongye Southern Engineering Technical Co., Ltd, office and technical research building of Zhongtie 11 bureau, landscape design for the Xiangshan Minyuan developed by Yichang Hongxiang group Co., Ltd, planning for the Tingtao Guanhai district developed by Wuhan Hongyu Industry Co., Ltd and etc. all of the projects above got the good estimate form our clients.

公司成立三年以来，出色地完成了多项设计工作，其中包括武汉地产开发投资集团的汉口花园二期景观工程设计、武汉众联投资有限公司的众联国际小区的规划、建筑设计、武汉市华盈房地产有限公司的凤凰城、咸宁锦江旅业公司的楚天温泉假日大酒店、恩施日内瓦风情小区、五环科技股份有限公司新办公大楼景观设计、中冶南方工程技术有限公司总部景观设计、中铁十一局集团有限公司办公科研楼、宜昌弘洋集团公司的香山铭苑景观设计、武汉宏宇实业有限公司的听涛观海的规划、建筑与景观设计等项目，深受各开发商和业主的好评。

The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因，并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列，通过对克隆的降钙素序列的比较研究，结果显示同一科的鱼降钙素序列保守性较高，同时，根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究，结果显示在分类学上，分类地位较远的鱼，其降钙素相似性较低，分类地位越接近的鱼，其降钙素相似性越高；我们利用谷胱甘肽S-转移酶(Glutathions S-transferase，GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究，应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索，并将纯化的融合蛋白作为抗原，获得了高效价的兔抗鲑鱼降钙素免疫血清；生物活性研究表明，大肠杆菌表达的融合蛋白具有显著的降血钙作用。

According to the studied results at present, the studied objects are mainly thin plate and beam about sound radiation mode, a method computed sound radiation mode of complex structure is presented in this dissertation, according to the results of the previous two chapter, a theoretical method to solve acoustical radiation mode and acoustical radiation efficiency of complex structure by boundary element method and acoustical radiation theory is posed, the acoustical radiation power is expressed as a Hermitian quadratic form, while the radiation modes is determined by general eigenvalue decomposing, and then the radiation efficiency is computed via the orthodoxy of radiation modes with regard to impedance matrix and average velocity matrix; lastly, the validity of the method is proved by pulsating sphere and radiating cube with analytical results. According to results of the form three chapter, the structure sound radiation sensitivity is studied in detail, the sound radiation power of structure can be expressed as positive Hermitian quadratic form, the sound radiation sensitivity can be expressed as two parts by partial differential with respect to design variable, which are sensitivity of boundary velocity and impedance matrix, combined with the theory of FEM and BEM, the structure sound radiation can be translated to the analysis of structure dynamic sensitivity and impedance matrix sensitivity. The theory posted in this dissertation is tested by the FEM software ANSYS and the software AAS programmed by author.

在前几章的基础上，通过结构声辐射的模态理论对结构的声辐射的机理进行了深入地探讨，针对目前声辐射模态的研究对象主要是简单的板和梁类结构，提出了一种计算复杂结构声辐射模态的方法，利用前两章研究所得的结论，将边界元方法与广义特征值的理论结合起来研究了复杂结构的声辐射模态与声辐射效率，先将结构的声辐射功率表示为一个正定的厄米特二(来源：ABb7C论文0909网www.abclunwen.com)次型，运用广义特征值分解求解了复杂结构的声辐射模态，然后利用声辐射模态关于阻抗矩阵与均方速度耦合矩阵的正交性，求解了复杂结构的声辐射效率，最后用具有解析解的脉动球与辐射立方体验证了该方法的有效性。

Geek in the pink Well let the geek in the pink take a stab at it If you like the way I'm thinkin' baby wink at it I may be skinny at times but I'm fat fulla rhymes Pass me the mic and I'm a grab at it well isn't it delicious crazy way that I'm kissin' Cause baby listen to this don't wanna miss it while it's hittin' Sometimes you gotta fit in to get in But don't ever quit cause soon I'm gonna let you in but see I don't care what you might think about me You'll get by without me if you want I could be the one to take you home Baby we could rock the night alone If we never get down it wouldn't be a let down But sugar don't forget what you already know I could be the one to turn you out We could be the talk across the town Don't judge it by the color, confuse it for another You might regret what you let slip away Like the geek in the pink pink pink Like the geek in the pink yeah Well this relationship fodder don't mean to bother nobody But Cupid's automatic musta fired multiple shots at her Because she fall in love too often that's whats the matter At least I talk about it keep a pattern of flattery and She was starin' through the doorframe and eyeing me down like already a bad boyfriend Well she can get her toys outta the drawer then Cause I ain't comin' home I don't need that attention, see I don't care what she might think about me she'll get by without me if she want I could be the one to take her home Baby we could rock the night alone If we never get down it wouldn't be a let down But sugar don't forget what you already know I could be the one to turn you out We could be the talk across the town Don't judge it by the color, confuse it for another You might regret what you let slip away Hey baby look at me go From zero to hero You better take it from a geek like me I can save you from unoriginal dum-dums Who wouldn't care if you com...plete him or not So what I've got a short attention span A coke in my hand Because I'd rather have the afternoon, relax and understand My hip hop and flip-flops well it don't stop with the light rock A shot to mock you kinda puts me in the tight spot The hype is nothing more than hoo-ha so I'm Developing a language and I'm callin' it my own So take a peek into the speaker and you'll see what I mean That on the other side the grass is greener I don't care what you might think about me You'll get by without me if you want I could be the one to take you home Baby we could rock the night alone If we never get down it wouldn't be a let down But sugar don't forget what you already know I could be the one to turn you out We could be the talk across the town Don't judge it by the color, confuse it for another You might regret what you let slip away

怪胎的粉红色那么让怪胎的粉红色就在它刺伤如果你喜欢的方式我thinkin '婴儿在这眨眼我可能有时瘦，但我胖fulla韵我的通行证麦克风和我是一个抓住它以及是不是疯了美味的方式，我基辛'原因婴儿收听此不想错过它，而它的hittin '有时候，你总得适应进入但是，不要任何时候都很快退出事业我一定让你看到的，但我不在乎你们可能认为我你会得到的没有我如果你想我可以是一个带您回家宝贝我们可以岩夜间单独如果我们永远不会下跌不会是一个放下但是，糖不要忘记你已经知道我可以是一个把你我们可以谈论整个镇不要判断它的颜色，它混淆另一你可能会感到遗憾的是你让溜走像怪胎的粉红粉红粉红像怪胎在粉红色的是那么这种关系的饲料并不意味着没有人打扰但丘比特的自动穆斯塔发射多种拍摄她因为她爱上往往是什么问题至少我谈论这件事保持一贯奉承和她starin '通过门框和关注我已经想了一个坏男友那么她可以让她的玩具outta然后抽屉原因我不是comin家里我不需要注意，见我不在乎什么，她可能认为我她会没有我的，如果她想我可以是一个带她回家宝贝我们可以岩夜间单独如果我们永远不会下跌不会是一个放下但是，糖不要忘记你已经知道我可以是一个把你我们可以谈论整个镇不要判断它的颜色，它混淆另一你可能会感到遗憾的是你让溜走喂婴儿看我走从零到英雄您是否可以更好地从一个像我这样的怪胎我可以为您节省来自非独创的达姆， dums 谁不会关心你的COM 。。。他完全或不所以我有一个短期注意跨度焦炭在我的手因为我宁愿有下午，放松和理解我的嘻哈和触发器以及它不停止与轻摇滚一杆嘲笑你有点儿让我在紧张的现场宣传只不过是豪哈所以我开发的一种语言，我callin '但我自己因此，需要看到的发言，你会明白我的意思这在另一边的草绿色我不在乎你们可能认为我你会得到的没有我如果你想我可以是一个带您回家宝贝我们可以岩夜间单独如果我们永远不会下跌不会是一个放下但是，糖不要忘记你已经知道我可以是一个把你我们可以谈论整个镇不要判断它的颜色，它混淆另一你可能会感到遗憾的是你让溜走

By virtue of his achievements, a basic regression equation is deduced showing how the government officials incorruptness promotes the economic development, and an estimation list is worked up to show how the economic development driven by incorruptness. The list proves that if a standard deviation is increased by the degree of incorruptness, the per capita GDP growth rate will increase by 0.3%, the education investment in GDP will increase by 0.23% and the ratio of social welfare expenditure to GDP will increase by 1.56%.

Mauro，借助Mauro的研究成果，归纳出廉洁促进经济发展的基本回归方程，并制作了清廉度促进经济发展的估算表，清廉度每提高一个标准差可使"人均GDP增长率"增加0.3个百分点，"教育投资占GDP的比例"增加0.23个百分点，"社会福利支出占GDP比例"增加1.56个百分点。

The conversion boundary of self-incompatibility was researched by sowing by stage. Sunshine of day and temperature was studied critically by investigating leafage and researching traits indoors. The results showed that the daily minimum temperature was the principal factor of affinity conversion and the affinity conversion boundary was 16.5~18.1℃.HE97 was primarily regard as self-incompatible line in this research. The method was by inbreeding and sib HE97, Half the silk of HE97 and Zheng58. Inbreeding and cross have been done simultaneously. The extending condition of pollen tube was observed by dying the silk after pollinating 4 hours. The individuals with pollen tube were observed having seeds, while the individuals without pollen tube were observed having few seeds or no seeds. So this study regard HE97 as sporophytic self-incompatibility .The planting ration of female parent HE97 and male parent 87-1 was 4:1.The nature cross-pollination was studied by the theory of endosperm xenity, the study show that the setting percentage of HE97 was 625%, in which the seed in self was 70% and the seed in hybrid was 30%. This was consistent with the study of silk anatomy structure, then come to the same conclusion: compatible pollen germination and fertilization can stimulate the self-incompatible pollen germination and fertilization in the same corn cob.

通过分期播种，进行了光温敏自交不亲和材料HE97亲和性转换区间的研究，依据田间跟踪调查叶龄及室内考种研究了HE97亲和性转换区间的光温临界值，结果表明：1日最低温度是影响HE97自交不亲和性的主要原因，亲和性转换区间在16.5℃～18.1℃之间。2通过HE97自交和姊妹交以及将HE97与郑58作为双亲，各自的花丝一分为二，分别正反交，授粉后4个小时分别取其花丝进行固定、染色，观察花粉管的伸长情况，根据结实率与花粉管伸长的对应关系，结果发现观察到花粉管的对应植株收获到了种子，而没有观察到花粉管的对应植株结实率极低或没有结实，本研究把HE97初步划为孢子体自交不亲和类型。3以HE97为母本，87-1为父本，按4母：1父行比，通过花粉直感效应研究天然异交率，结果母本HE97结实良好(62%)，其中自交粒占70%，异交粒占30%，结合花丝切片观察表明，亲和花粉的萌发及受精会导致落在同一果穗柱头上不亲和花粉的萌发不再受抑制。

By rose, by satiny bosom, by the fondling hand, by slops, by empties, by popped corks, greeting in going, past eyes and maidenhair, bronze and faint gold in deepseashadow, went Bloom, soft Bloom, I feel so lonely Bloom.

566从玫瑰花、裹在缎衣里的酥胸、爱抚的手、溢出的酒、以及砰的一声崩掉的塞子旁边，布卢姆一面致意一面走过去，经过一双双眼睛，经过海绿色荫影下的褐色和淡金色的处女发。

"That the doctrine of the Reformed churches concerning predestination, and the points annexed to it, by its own genius and necessary tendency, leads off the minds of men from all piety and religion; that it is an opiate administered by the flesh and by the devil, and the stronghold of Satan, where he lies in wait for all; and from which he wounds multitudes, and mortally strikes through many with the darts both of despair and security; that it makes God the author of sin, unjust, tyrannical, hypocritical; that it is nothing more than interpolated Stoicism, Manicheism, Libertinism, Turcism; that it renders men carnally secure, since they are persuaded by it that nothing can hinder the salvation of the elect, let them live as they please; and therefore, that they may safely perpetrate every species of the most atrocious crimes; and that, if the reprobate should even perform truly all the works of the saints, their obedience would not in the least contribute to their salvation; that the same doctrine teaches, that God, by a mere arbitrary act of his will, without the least respect or view to sin, has predestinated the greatest part of the world to eternal damnation; and, has created them for this very purpose; that in the same manner in which the election is the fountain and cause of faith and good works, reprobation is the cause of unbelief and impiety; that many children of the faithful are torn, guiltless, from their mothers' breasts, and tyrannically plunged into hell; so that, neither baptism, nor the prayers of the Church at their baptism, can at all profit by them;" and many other things of the same kind, which the Reformed Churches not only do not acknowledge, but even detest with their whole soul.

"该学说的革新教会联谊会关于宿命，并分附在它，由它自己的天才和必要的倾向，导致起飞的头脑男子从所有虔诚与宗教；这是一个鸦片类由血肉所魔鬼和据点撒旦，在那里他是在等待所有；从他的伤口众多，而致命打击，通过许多与飞镖都绝望与安全；因为它使上帝的作者单，不公正，专横，虚伪的，即它只不过是多插生活俭朴，摩尼教，放纵， turcism ；这使得男性的肉体关系稳固，因为它们是说服它说，没有任何东西可以阻碍救赎的选举，让他们住，因为他们请；因此，他们可以安全地胡作非为，每一个物种的最残暴的罪行，并认为，如果reprobate甚至应该真正履行全部工程的圣人，他们的服从，将不会在至少贡献出他们的救恩，即同一教义教导说，上帝，由一个单纯的任意行为，他的意志，如果没有起码的尊重，或以单，有predestinated最大的是世界的一部分，以永恒damnation ，并且创造了，他们为了这个目的，即在同一何种方式选举是喷泉和事业的信念和良好的工程， reprobation ，是导致不信者和不虔诚；说，许多儿童的教友们被拆除，无罪，从他们的母亲的乳房，并tyrannically陷入地狱，因此，既不洗礼，也没有祷告的教会，在他们的洗礼，能在所有利润由他们来主宰；"和许多其他东西的同类，而改革教会不仅不承认，但即使是讨厌自己的整个灵魂。

Based on the elastic theory of large deflection and by taking into account the variation of the axial force and its effects on bending deformation of the drillstring in vertical section,the vibration of the drillstring induced by fluctuating weight-on-bit is described by a parametrically excited system.A Mathieu equation describing the system is established by Galerkin Method.By extending the solution into a Fourier series,the curves of dynamic bifurcation values and the dynamically unstable regions surrounded by the curves are presented.

基于弹性大变形理论，考虑到轴力沿钻柱轴线的变化及其对钻柱弯曲变形的影响等因素，将受自重作用的铅垂井段钻柱在波动钻压激励下的振动转化为一个参数激励系统，用Galerkin方法首次得到了描述此系统的Mathieu方程，并通过将方程的解展成富里叶级数的方法，得到了该振动系统的动力分岔值曲线及其所包围的动力不稳定区。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。