查询词典 by-road

This book is attributed to a group of writers:Errors in the use of Auxiliary Verbs by Han Yufang,Adverb才and就by Zhou Qingming,Conjunction和by Han Xiaoping,Particle吗by Cui Fuying,Adverbial Adjuncts by Hu Zhiying,Complements of Frequency by Du Tonghui,Peng Zhiping,Li Xiuqing,Complements of Duration and跟…一样by Li Chengcai,Complex Sentences of Suppositional,Conditional,Causative and Adversative Relation by Dong Yuan,Rhetorical Question by Wang Xiuyun,and the rest by Li Zhu,Cheng Meizhen.

本书能愿动词误用由韩玉芳执笔；副词"才"和"就"由朱庆明执笔；连词"和"误用由韩孝平执笔；助词"吗"误用由崔福英执笔；状语误用由胡志英执笔；动量补语误用由杜同蕙、彭志平、李秀清执笔；时量补语和"跟……一样"误用由李成才执笔；假设、条件、因果和转折关系复句的误用由董原执笔；反诘句误用由王秀云执笔；其余的部分由李珠、程美珍执笔。

I was also helped by James Carville and a brilliant campaign team who kept me and everyone else focused and on message through all the ups and downs; by Stan Greenberg's insightful polling and Frank Greer's effective paid media; by able people who led the campaign at the grass roots; by a Democratic Party united by Ron Brown's skill and the desire to win after a dozen years in the wilderness; by extraordinarily high levels of support from minorities and women, who also elected a Congress with six female senators and forty-seven female members of the House, up from twenty-eight; by the initial disunity and overconfidence among the Republicans; by surprisingly positive press coverage in the general election, in stark contrast to the going-over I got in the primaries; by the extraordinary performance of Al and Tipper Gore in the campaign, and the generational change we all represented; and by the New Democrat philosophy and ideas I had developed in Arkansas and with the DLC.

多亏了詹姆斯。卡维尔和才华出众的竞选班子的帮助，无论是处于顺境还是逆境，他们使我和其他所有人精力集中在我们的政见上；也多亏了斯坦。格林伯格富有洞察力的民意调查和弗兰克。格里尔卓有成效的付费宣传手段；多亏了在基层领导竞选活动的能干的人们；多亏了因罗恩。布朗的领导技巧和12年在野之后希望重新上台的愿望而团结一致的民主党；还有来自少数族裔和妇女非凡的鼎力支持，是他们，还选举出了一个有着6名女参议员和47名女众议员的国会，以前只有28名；也得感谢共和党刚开始的不团结和过分自信；感谢大选中来自媒体的令人惊异的正面报道，与我在初选中遭到的严厉谴责形成鲜明的对比；感谢艾尔和蒂珀的杰出表现，以及我们所有人代表的新老两代人的交替；感谢我在阿肯色州及民主党领袖委员会工作期间形成的人生观和理念。

How then will it be when I think of the powerful good things which you have showered on me while I am not able to thank you enough for any one of them, so may the words of your feather be praised according to your knowledge and the judgments you have made and so much of your grace has encompassed your creatures and so much as your power has encompassed and a multiple that you are due from your creatures, oh Lord, I testify to my good deeds towards you, so add to your goodness to me for the rest of my life, greater, more complete, and even better than in the past with your mercy and you are the most merciful Lord, I implore you and beseech you by calling you the ONLY ONE, by glorifying you, praising you and acclaiming you, calling you the greatest, praising your completeness, by your leadership, by calling you the mightiest, by sanctifying you, by your glory, your mercy, your grace, your wisdom, your gentleness, your high position, you're your respect, your goodness, your exaltedness, your generosity, your perfection, your pride, by your rule, your might, your good acts, your thankfulness, your beauty, your grandeur, your proof, by your forgiveness, by your prophet and those close to you, his family and companions that you bless our Lord Mohammed and all other prophets and messengers and that you do not refuse me you care, nor your good deeds,… nor your beauty, nor your grandeur, nor the benefits of your honor, for you have given me so many gifts that you can never be hindered by miserliness, neither will neglect to thank you for your goodness reduce your generosity nor will your countless, exalted…… beautiful, noble gifts limit your overwhelming generosity, nor will you fear poverty and the river of your good deeds does not reduce your generosity, nor will you fear poverty and then become stingy.

当我想起你曾慷慨赐给我的好处，而对于每一个好处，我都无力以尽谢，愿你按你的智慧和你所成就的审判得着赞美。你以丰盛的恩惠环绕你所造的，正如你的力量环绕他们一样，你当从你所造的得到众多的赞美。主啊，我向你证实我对你的善功，所以在我的余生求你比以前更大，更全更好地以怜悯仁慈待我，你是至慈的主。我恳求你，哀求你，称你为至大，赞你的完全，你的带领，称你为至强的主，尊崇你，赞美你的荣耀，怜悯，恩惠，你的智慧，亲切，高位，你的仁慈，尊贵，慷慨，你的完美，统治，力量，善举，你的美丽，高贵，你的证明，你的宽恕。愿你赐福我们的至圣穆罕默德和他的家人，伙伴和所有其他的先知和使者以及接近你的人。你没有拒绝看顾我，也没有停止你为我做的美事，对我的礼待恩惠，因你给我如此之多的礼物，毫不吝啬，我也不会忘记感谢你的仁慈。你巨大的慷慨不会只限于无数尊贵，美丽，极好的礼物，纵然你的美事成川，你的慷慨也不见少，你也不会害怕贫穷，以至吝啬。

Snow is falling from the sky in the middle of July The sun was shining in my eyes again last night It all goes off without a sound, the silence is so loud Something isn't right Footsteps echo down the hall, no one's there at all Dial your number but your voice says "I'm not home" Everything is inside out, don't know what it's about It keeps getting Stranger by the day Stranger by the day It keeps getting stranger by the day Stanger by the day, yeah Going for a walk outside, to see what I can find No reflections in the window as I pass by It feels hotter in the shade, water running from the drain Something going on Coversations with a mime,(?????????alguem sabe) Imagination must be working overtime The world is upside-down, everything is turned around It keeps getting, Stranger by the day Stranger by the day It keeps getting stranger by the day Stanger by the day, yeah By the time I reach your door, I can't take any more I just happened to be in your neighborhood I'm the one who gets surprised, I Don't believe my eyes Your alibi's no good.

雪是从空中向下，在7月中旬阳光明媚，在我的眼睛再次昨晚它一切顺利起飞，没有一个健全，沉默是如此响亮东西是不正确的脚步声回波下来大厅，没有人的存在，在所有拨打你的电话号码，但你的声音说："我没有家"一切都是里面出，不知道这是什么，它的约它总是陌生的，一天陌生的，一天它总是有陌生人一天陌生的，一天，是啊要设立一个走在外面看到有什么我可以了解没有思考，在窗口我擦肩而过它感到热，在阴凉处，水从排水管一些正在进行 coversations与默剧，）想象力必须超时工作世界是倒置式的，一切扭转它总是，陌生的，由天陌生的，由天它总是由陌生人一天 stanger由一天，是啊由那时，我到达你的门，我不能采取任何更多我只是刚巧在你的周边环境我是一个谁得到惊讶，我不相信我的眼睛你的不在犯罪现场的任何好处。

Effects of dry temperature and dry time on properties of heat cured sand and the relationship with binder composition are as follows:sands bonded by binders that are not modified have high strength when dry temperature is high and dry time is long,but their anti-wettability power is poor . Sands bonded by binders that are modified by B but not modified by M can be dried quickly and sand strength is high but anti-wettability power is also poor. Sands bonded by binders that are modified by M but not modified by B can also be dried quickly to obtain maximum strength,then the strength becomes lower when they are dried continually for more time. and sand strength is high but anti-wettability power is also poor. Sands bonded by binders that are modified by M as well as by B at the same time can be dried quickly to obtain maximum strength at low temperature and short time. But the anti-wettability of sand mold is best when they are dried at high temperature and long time.

烘干温度和烘干时间对热硬砂性能的影响及其与粘结剂改性的关系是：未经改性剂B和改性剂M改性的粘结剂砂，脱水速度较慢，烘干温度越高，时间越长，则硬化强度也越高，但未经改性的粘结剂热硬砂的抗吸湿性较差；经改性剂B而不经改性剂M改性的粘结剂热硬砂，烘干硬化速度较快，强度也高，但在高湿度下的抗吸湿性不好；经改性剂M改性而不经改性剂B改性的粘结剂热硬砂，烘干时强度先随烘干时间升高至最大值后，继续烘干时强度又出现下降；经改性剂B和经改性剂M共同改性的粘结剂热硬砂能在短时间和较低的烘干温度下就可烘干到最高强度，即烘干脱水的速度较快，但试样的抗吸湿性差，若在更高温度和更长时间下烘干，试样的抗吸湿性得到改善，综合考虑烘干强度和抗吸湿性要求，对于φ30×30mm试样，理想的烘干温度是200℃，烘干时间20分钟。

With the advice and unanimous consent of our brothers, cardinals of the holy Roman church, by means of this our constitution which will have permanent validity, we establish, ordain, decree and define, by apostolic authority and the fulness of our power, that if it happens (which may God avert in his mercy and goodness towards all), after God has released us or our successors from the government of the universal church, that by the efforts of the enemy of the human race and following the urge of ambition or greed, the election of the Roman pontiff is made or effected by the person who is elected, or by one or several members of the college of cardinals, giving their votes in a manner that in any way involves simony being committed -- by the gift, promise or receipt of money, goods of any sort, castles, offices, benefices, promises or obligations -- by the person elected or by one or several other persons, in any manner or form whatsoever, even if the election resulted in a majority of two-thirds or in the unanimous choice of all the cardinals, or even in a spontaneous agreement on the part of all, without a scrutiny being made, then not only is this election or choice itself null, and does not bestow on the person elected or chosen in this fashion any right of either spiritual or temporal administration, but also there can be alleged and presented, against the person elected or chosen in this manner, by any one of the cardinals who has taken part in the election, the charge of simony, as a true and unquestionable heresy, so that the one elected is not regarded by anyone as the Roman pontiff.

的意见，并一致同意，我们的兄弟，红雀的罗马教会，通过我们的宪法，这将永远有效，我们建立，注定，法令和确定，由使徒权威和丰富我们的权力，如果发生（可能避免上帝在他的仁慈和善良对所有），上帝已发布后我们或我们的继任政府的普世教会，这所作出的努力的敌人，人类的欲望和以下的目标或贪婪，选举是罗马教皇或影响的人谁当选，或由一个或几个成员的枢机主教，使他们的选票的方式，以任何方式涉及西摩尼正在致力於-的礼物，许诺或收到的资金，货物的任何类型，城堡，办事处， benefices ，承诺或义务-选出的人或由一个或几个其他人，以任何方式或形式，即使选举产生了多数三分之二或一致选择所有红雀，甚至在一种自发的协议的一部分，所有，未经审核作出，那麼不仅是本次选举或选择本身无效，并没有赋予人选举或选择以这种方式的任何权利或者精神或时间管理，而且还可以有指控，并提出，对当选者，或选择以这种方式，任何一个红雀谁参加了选举，充电的西摩尼，作为一个真正的和不容置疑的邪说，使选举是一个任何人都不会认为是罗马教皇。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Kw=%B2%DC%EA%D8%CE%C4 Name: Xi Cao, Name: Caoxue Height: 164 cm Weight: 46 kg Date of Birth: November 16 Blood type: O - Countries or regions: China Origin: Hangzhou Education: Central Drama学院表演系2002 Constellation: Scorpio Hobbies: Kandie, reading Features: dancing, singing and playing, horseback riding, swimming, piano Favorite food: sweets Favorite singer: Celine Dion Favorite actress: Georgia Winnie Sipateluo Favorite movie:"Calgary - Cities chocolate" Favorite color: red, black Most want to do: travel Major film and television works: 2001 "on the old" played by Xiao Director: Li Qiyuan cooperation Cast: Ouyangzhenhua Niuben 2001 "Xiaoshiyilang" decorated clove Director: Li Wenyan cooperation Cast: Athena Chu Wu Qilong 2002,"provincial party secretary," played by small-Jan Director: Su Zhou cooperation Cast: Wu Gang Du Yulu 2002 "Woman Soup" played by Huang Pei-yun Director: Hu Xiulan cooperation Cast: Zhang 2002 "Red Mansion Yatou" decorated Sheyue Director: Huang Jianzhong cooperation Cast: Li Qian Fang Qingzhuo 2002 "Gengu surprising reprisal" showcase Director: Su Zhou cooperation Cast: Tao Zeru SU Jin Han Ying quiet Du Yulu 2003 "Zuozhuang" played by Huo Xin Director: Qi Jian cooperation Cast: Wang Qian Xi Meijuan 2003 "six women and pawn shops," played by Narcissus Director: Huang Kemin cooperation Cast: Wu Kuan Hu Jing Yan倩倩罗海琼 2003 "spring and summer autumn and winter," decorated Anping Director: Wang Xiaokang cooperation Cast: A duo Weizi 2003 "definitely" played by Xiaolan Director: Yang Wenjun cooperation Cast: HU Bing, Zhang Ting Huang 2004 "Love in the left and the right situation," decorated Qiaobei Director: Jin Chen cooperation Cast: Tianzhonglinai (Sino-Japanese co-idol drama) 2004 "sidetracks turnouts who" played by small Kivu Director: high Chichi cooperation Cast: Li Xuejian 2005,"Liu Xia Shujian situation changed three" played by show - Director: Chen Haowei cooperation Cast: Lin Zhiying Ho Tian Hu can afternoon Ma Li Zonghan 2005 "walk the Jimaodanzai" decorated Sanfu Director: Following the dream of cooperation Cast: Kou Shixun Pan Hong Wang Zhifei 2005 "Malapoxi" decorated Zhonghui Director: Zeng Nianping cooperation Cast: Gui Yalei He Lin Wu Jun Zengbao Yi Lin Shen 2006 "Five-Star Hotel," played by Yang Yue Director: Liu Xin Gang cooperation Cast: Meng Meng Niu Cheng Chun-ling blend 2006 "sorrow at the Changshou Ge" showcase beautiful Director: Wu Yi Juan cooperation Cast: Huang Lei Jia Jingwen Pan Hong Shen Xiao Hai 2006,"Wu Yue Wang Qian," decorated WU Qiao Niang Director: Hu Kai cooperation Cast: Nicolas Massu Xiu-qing Wang Yanan 2006,"Qi Nvzai of Liaozhai Xianv" played by Lin Fu, Director: National cooperation Cast: Daniel Chan Lin Yu-SUN Xing 2007 "sister" played by Zhu Xiuling Director: Mr consistent cooperation Cast: should children Qinpei Chun-Ning Huang Yi-fei Major theatrical works:"Yu Tangchun" played by actress Su "Zhen Zhushan" played by women on the 2nd Qinger Major advertising works: Yangsheng Tang Nongfuguoyuan "shake a shake" Di rats electric bicycle ads Extensive digital camera image speak Luolai home textiles brand new "Shang" Star Profile: 2003 graduated from the Central Academy of Drama, the Xi Cao, is the first show in 2001 "on the old", that was a adolescent girls.

姓名：曹曦文原名：曹雪身高：164cm 体重：46kg 生日：11月16日血型：O型国家或地区：中国大陆籍贯：杭州学历：中央戏剧学院表演系2002 星座：天蝎座爱好：看碟、阅读特长：唱歌、跳舞、打球、骑马、游泳、钢琴喜爱的食物：甜食喜爱的歌手：席琳迪翁喜爱的演员：格温妮斯帕特罗喜爱的电影：《浓情朱古力》喜爱的颜色：红，黑最想做的事：旅游主要影视作品： 2001 年《上有老》饰李笑导演：李启源合作演员：欧阳震华牛犇 2001 年《萧十一郎》饰丁香导演：黎文彦合作演员：吴奇隆朱茵 2002 年《省委书记》饰马小扬导演：苏舟合作演员：杜雨露巫刚 2002 年《女人汤》饰黄培芸导演：胡秀兰合作演员：张延 2002 年《红楼丫头》饰麝月导演：黄健中合作演员：李倩方青卓 2002年《亘古奇冤》饰导演：苏舟合作演员：陶泽如苏瑾韩影宁静杜雨露 2003 年《坐庄》饰霍昕导演：戚健合作演员：奚美娟王茜 2003 年《六女当铺》饰水仙导演：黄克敏合作演员：邬倩倩罗海琼严宽胡静 2003 年《春夏秋冬》饰安平导演：王小康合作演员：巍子阿朵 2003 年《绝对计划》饰小兰导演：杨文军合作演员：胡兵黄奕张庭 2004 年《爱在左，情在右》饰乔贝导演：金琛合作演员：田中丽奈 2004 年《搭错车》饰小伍导演：高希希合作演员：李雪健 2005年《书剑情侠柳三变》饰秀香导演：陈浩威合作演员：林志颖何美钿胡可午马李宗翰 2005 年《行走的鸡毛掸子》饰三伏导演：梦继合作演员：寇世勋潘虹王志飞 2005 年《麻辣婆媳》饰钟慧导演：曾念平合作演员：归亚蕾何琳吴军曾宝仪林申 2006 年《五星大饭店》饰杨悦导演：刘心刚合作演员：张峻宁牛萌萌郑柔美 2006 年《悲伤时唱首歌》饰明媚导演：胡意涓合作演员：黄磊贾静雯潘虹沈晓海 2006 年《吴越钱王》饰吴巧娘导演：胡凯合作演员：王亚楠马苏修庆 2006 年《聊斋奇女子之侠女》饰林月芙导演：李国立合作演员：陈晓东林家宇孙兴 2007 年《香港姊妹》饰朱秀玲导演：麦贯之合作演员：应采儿秦沛张峻宁黄一飞主要舞台剧作品：《玉堂春》饰女主角苏三《珍珠衫》饰女二号晴儿主要广告作品：养生堂农夫果园&摇一摇&迪鼠电动自行车广告广博数码相机形象代言罗莱家用纺织品新品牌&尚&明星简介： 2003年毕业于中央戏剧学院的曹曦文，第一部戏是2001年的《上有老》，那是一个青春期女孩。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Death Metal:"Disposal of the Body" by Cannibal Corpse,"Homage for Satan" by Deicide,"Post Mortal Ejaculation" by Cannibal Corpse,"The Exorcist" by Possessed,"The Optimist" by Skinless,"4:20" by Six Feet Under,"Stabwound" by Necrophagist,"Egypt, the Red Earth" by Necronomicon,"Buried By The Dead" by Bloodbath,"Sick Salvation" by Bloodbath,"Prometherion" by Behemoth,"Scrolls of the Megilloth" by Mortification,"Demon of the Fall" by Opeth.

死亡金属：&该机构处理&的吃人尸体，&为撒旦致意&的弑神者&，射精后红尘&的吃人尸体，&大法师&的着魔，他又说：&去皮乐观&，&16：20：00&的六英尺下&，Stabwound&的Necrophagist，&埃及，红地球&组合成员，他说：&陪葬&的大屠杀，&病态救国&的大屠杀，&Prometherion&的巨兽&的Megilloth卷轴&的禁欲&恶魔的堕落&的Opeth。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。