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Prussian blue staining revealed more than 98% of BM-MNCs were labeled by Feridex and BM-MNCs appeared to be unaffected by magnetically labeling. Three dogs injected Feridex- BM-MNCs were visualized at one week by MRI, but none of unlabeled BM-MNCs were seen. All lesions that were detected within one week could not be visualized over four week. The dot injected Feridex-cells which fixated by 4 % methanal also was seen at one week by MRI, but the signal disappeared at two week.

普鲁士蓝染色显示体外Feridex标记BM-MNCs成功率达98%以上，而且细胞活性不受影响；其中3只实验犬标记细胞移植区1周内可用1.5T磁共振仪3DSSFP序列在体扫描到T2低信号影像，4周时该低信号消失；同样条件下经甲醛固定的标记细胞在1周时也可检测到T2低信号，但2周时该低信号消失；未标记细胞移植区没有检测到低信号。

C By the end of the past year, he had read by night by a street lamp a novel by Dickens and learned a lot by heart.

直到去年末，他已经读完了迪更斯的一部小说。他是在夜间在路灯下阅读的。他记住了其中的很多东西。

Finally, the grafting polymerization of textile materials initiated by microwave low temperature plasma was conducted. Knitted cotton fabric was grafted by AMPS first, then knitted cotton and terylene/cotton fabrics were grafted with AMPS/NIPA dualistic monomers respectively. By means of grafting polymerization, the P dualisticintelligent hydrogel was added to textile.The influences of the factors on add-on, such as treatment time and power of plasma, concentrations of crosslinking agent and total monomers, proportion of AMPS, polymerizing temperature and time etc, was studied fully. The optimal conditions of grafting polymerization initiated by microwave low temperature plasma were determined by orthogonal design, variance analysis and mathematical statistics method so as to analyze the main influencing factors. Then swelling/deswelling kinetics, temperature-sensitivity and pH-sensitivity of grafted textile were studied. The performance indexes of hydrostatic pressure, moisture permeability and bursting strength of the knitted cotton and terylene/cotton fabrics before and after grafting were compared and evaluated synthetically.

此后用微波低温等离子体引发纺织材料的接枝聚合反应，分别研究了棉针织物接枝AMPS和棉、涤棉混纺针织物接枝AMPS/NIPA二元单体，使智能凝胶以接枝的方式添加到纺织材料上；详细探讨了微波低温等离子体处理功率和时间、总单体浓度、AMPS单体浓度、交联剂浓度及聚合反应温度和时间等因素对增重率的影响，通过正交设计、方差分析及数理统计对各影响因子进行分析，确定了微波低温等离子体引发接枝聚合的最佳工艺条件；对接枝纺织材料的失水-吸水动力学、温度敏感性、pH敏感性等进行了研究；对接枝前后棉、涤棉混纺针织物的静水压、透湿性和顶破强力等服用性能指标进行了对比和综合评价。

B communication by an establishment of a transmission or retransmission embodying a performance or display of a nondramatic musical work intended to be received by the general public, originated by a radio or television broadcast station licensed as such by the Federal Communications Commission, or, if an audiovisual transmission, by a cable system or satellite carrier, if

b一个设施传播或二次传播从一个得到像联邦通讯委员会这样的机构许可的广播或电视站播放的(如果是视听传输，则通过有线系统或卫星电波来播放)、旨在让大众接收到、含非戏剧音乐作品的表演或展示的行为，如果--。

Communication by an establishment of a tra mi ion or retra mi ion embodying a performance or di lay of a nondramatic musical work intended to be received by the general public, originated by a radio or television broadcast station lice ed as such by the Federal Communicatio Commi ion, or, if an audiovisual tra mi ion, by a cable system or satellite carrier, if

一个设施传播或二次传播从一个得到像联邦通讯委员会这样的机构许可的广播或电视站播放的（如果是视听传输，则通过有线系统或卫星电波来播放）、旨在让大众接收到、含非戏剧音乐作品的表演或展示的行为，如果

West Virginia is bordered by Pennsylvania to the north; by Ohio to the north and west; by Kentucky to the west; by Maryland to the north and east; and by Virginia to the east and south.

西弗吉尼亚州接壤的东北部宾夕法尼亚州；由美国俄亥俄州的北部和西部；由肯塔基州以西的马里兰州的北部和东部地区；和弗吉尼亚州东部和南部。

Gothic novels -- mostly stories of mystery and horror which take place in some haunted or dilapidated Middle Age castles -- were turned out profusely by both male and female writers; works such as The Castle of Otranto (1765) by Horace Walpole, The Mysteries of Udolpho (1794) and The Italian (1797) by Mrs. Ann Radcliffe, The Champion of Virtue, a Gothic Story (1777) by Clara Reeve, and The Monk (1796) by M.G.

哥特小说--神秘的恐怖故事大多发生在一些破旧或中年古堡闹鬼--原来发牢骚的人，男性和女性作家；作品如城堡奥嘉沃波尔(1765)， udolpho谜(794)和意大利(1875年)由夫人安安拉德克利夫，冠军美德一个哥特故事(777)克拉拉白冠、和尚m.g。

In another word, the status does not exist that a painter should learn from nature "purely" without any "prejudice" and paint realistically by facing an object to paint out what he or she has perceived. To obtain such capability, painting learners or students shall use their eyes (teachers ask students in the painting class "where are your eyes and your perceptions") to observe objects and things at the beginning of study and then paint the models faithfully by means of "facade-subdivision"(or "preferring to square not round" as described by Xu Beihong, or decomposing the object into many petty facades as described in the line drawing textbook by P.P. Chistjakov so as to turn around the object bit by bit).

也就是说，不存在这样一种状态，画家为了画出自己的感觉，他们应该"纯洁"地不带"偏见"地去向自然学习，去面对物体直截了当地写生，而为了获得这种能力，在学习绘画的初始阶段，就必须训练学生们用自己的眼睛(在绘画课室里，老师总是质问学生，你的眼睛在哪里，你的感觉在哪里)去观察对象，然后用一种"分面"的方法(也就是徐悲鸿所说的"宁方勿圆"，或者按照据说是契斯恰科夫的素描教程的要求把对象分解成许多小面，以便使物体能够一点一点地转过去的方法)把眼前的模特如实地画下来。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

The Shah Dynasty found then lasted until now. Nepal was invaded by Britain in 1814 and forced to cede a vast expanse of territory to India which was controled by Britain at that time. Its diplomacy was supervised by Britain as well. During the period from 1846 to 1950 Nepal was controled by Rana Family . The fight aganist it intrigued by Nepali Congress brought the dormination of Rana Family to an end, and constitutional monarchy was put into practice. In February of 1959 ,Mahemdra Bir Bikram Shah Dev issued the first constitution of Nepal, the New Constitution was issued in 1962. Birendra , Bir Bikram Shah Dev ascended throne in1972, he dismissed the National Panchayat in the April 16, 1990,and he also issued the third constitution in Nov ember of the same year. Then the multi-party of constitutional monarchy was set up.

建立了沙阿王朝，延续至今。1814年英国入侵，尼泊尔被迫割让大片领土给英属印度，外交受英监督。1846年―1950年为拉纳家族统治时期。1950年11月，尼泊尔大会党等发起反拉纳的斗争，结束了拉纳的统治，并实行君主立宪制。1959年2月马亨德拉颁布尼泊尔第一部宪法。1962年颁布新宪法。1972年比兰德拉国王即位。1990年4月16日，比兰德拉国王解散全国评议会，同年11月颁布第三部宪法，实行君主立宪的多党制。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。