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The goal of cities which try to be creative is to create conditions which are open enough so urban decision makers can: Rethink potential such as turning waste into a commercial resource; revalue hidden assets such as discovering historic traditions that can be turned into a new product; reconceive and remeasure assets such as understanding that developing social capital also generates wealth; reignite passion for the city by for example developing programmes so people can learn to love their city; rekindle the desire for learning and entrepreneurship by for instance creating learning modules much more in tune with young peoples' desires; reinvest in your talent by not only importing outside talent but fostering local talent; reassess what creativity for your city actually is by being honest about your obstacles and looking at your cultural resources afresh; realign rules and incentives to your new vision rather than seeing your vision as being determined by existing rules; reconfigure, reposition and represent where your city stands and by knitting the threads together to retell your urban story that galvanises citizens to act.

城市发挥创意的目的在於，创造足够开放的情境，让城市决策人士能够做到如下的事项：重新思考各项潜在可能，例如将垃圾转化成商机；重新赋予隐性资源价值，例如将历史传统转化为新的产品；重新构想、衡量资源，例如认知到发展社会资源，同时也能造就财富；重新点燃城市的热情，例如策划活动，让大家学习爱自己的城市；重新挑起学习与创新的欲望，例如设计学习模式，贴近年轻人的脉动；重新投资自己的人才，不要只是依赖外国人才，而是培养在地人才；则新评估自己城市的创意为何，例如诚实面对自己的障碍，并从新鲜的角度来看待自己的文化资源；重新整合规范、动机，运用新的视野，而非被动受限於既定规范；重新装配、定位、呈现自己城市的现状，编织自己的都会故事，重新诉说，驱使市民采取行动。

The thesis consists of six chapters. In the first, the technologies of atomic force microscopy and of the measurement elasticity of biomolecules were introduced. In the second, the validity of VSPFM was confirmed by lift mode atomic force microscopy. In this chapter, the height of DNA was measured by lift mode atomic force microscopy, which demonstrated that the method of height measurement of biomolecules by VSPFM was correct and established the foundation of the method of measurement elasticity of biomolecules by vibrating mode scanning polarization force microscopy. In the third chapter, detailed work has been illustrated on the foundation of the method of measurement elasticity of biomolecules by VSPFM. And the compressive elasticity of DNA was measured. In fourth and fifth chapters, the method was applied in the measurement elasticity of proteins. Two proteins elasticity, fibre-like protein α-synuclein and global protein IgG, were measured by VSPFM, through which the method wound its way to the application of biomolecules. In last chapter, the final part of the thesis was a summary. A conclusion of the thesis and a self-comment on my work as a PhD candidate have been made, and expectation about the further works has been addressed.

本论文共分为六章，第一章，引言部分主要介绍了原子力显微镜技术及生物大分子弹性测量技术；第二章主要是VSPFM方法的正确性论证，介绍抬高模式原理，利用抬高模式原子力显微镜对DNA的高度进行测量，论证振动模式扫描极化力显微镜测量生物大分子的高度的正确性以及准确性，从而为振动模式扫描极化力显微镜测量生物大分子的弹性方法的建立奠定基础；第三章以脱氧核糖核酸为例详细介绍了振动模式极化力显微镜测量生物大分子弹性的方法的建立，对DNA的压弹性进行了初步的测量和分析；第四章和第五章介绍了振动模式扫描极化力显微镜在蛋白质弹性测量中的应用：α-synuclein和IgG分别是纤维状蛋白和球状颗粒蛋白，通过振动模式扫描极化力显微镜测量这两种蛋白质的弹性，摸索振动模式扫描极化力显微镜在蛋白质弹性测量中的应用；第六章对全文进行了总结，在对论文的工作进行归纳和自我评价之后，还对进一步的工作进行了展望。

5S-160GP/9T-160 consists of a main case driven by 3-shaft fixed spindle and a rear mounted auxiliary case driven by planet gears. The main case has 5 forward speeds and 1 reverse speed. The auxiliary case has 2 speeds which double the speed of the main case, so that the transmission has 9 forward speeds, the 4 forward speeds of main case shift by ZF-BK short travel synchronizer, auxiliary case shift by lock ring synchronizer, crawler and reverse shift by join sleeve powered by the engine of 280-380 hp.

5S-160GP/9T-160型变速器由三轴传动的主变速器和后置的行星齿轮传动副变速器组成，主变速器有五个前进档和一个倒档，副变速器有两个档位，可将变速器的档位增加一倍，故变速器具有九前一倒档，主变速器4个前进档采用ZF-BK型短行程同步器换档，副变速器采用锁环式同步器换档，爬档和倒档采用接合套换档，用户可根据需要选装部分功率取力器N71 /1或N71/2， N70。

By K. Friar (1979); The Cretan Glance by MP Levitt (1980); Tormented by Happiness by P. Bien (1982); Kazantzakis: Politics of the Spirit by P. Bien (1988); The Last Temptation of Hollywood by LW Poland (1988); God's Struggler , ed.

克雷伯弗莱尔（ 1979年）； cretan一眼，由国会议员利维（ 1980年）；折磨，由幸福的，由体育bien （ 1982年）； kazantzakis ：政治的精神，由体育bien （ 1988年）；最后的诱惑，由好莱坞大白猪波兰（ 1988年）；上帝的事迹，教育署。

In order, however, that those who ought to attend so very useful a council may not be held back in any way from coming, we hereby grant and concede, with the approval of the said sacred council, to each and every one of those summoned to the council by the said Julius, our predecessor, or who ought to take part, by right or custom, in the meetings of general councils, especially those of the French nation, and to those schismatics and others who are coming to the said Lateran council by common or special right, on account of a declaration or apostolic letter of our predecessors or of the apostolic see (except, of course, those under prohibition), and to the attendants and associates of those who come, of whatever status, rank, condition or nobility they may be, ecclesiastical or secular, for themselves and all their belongings, a free, guaranteed and fully comprehensive safeconduct, for coming by land or sea through the states, territories and places that are subject to the said Roman church, to this Lateran council in Rome, and of residing in the city and freely exchanging views, and of leaving it as often as they wish, with complete, unrestricted and total security and with a true and unchallengeable papal guarantee, notwithstanding any impositions of ecclesiastical or secular censures and penalties which may have been promulgated in general against them, for whatever reasons, by law or by the aforesaid see, under any forms of words or clauses, and which they may in general have incurred.

为了然而，这些谁应该参加这样非常有用的一个委员会可能不会举行任何方式从今后，我们在此授予和承认，并核准说，神圣的理事会，每一个人传唤安理会的朱利叶斯说，我们的前任，或谁应该采取的一部分，由权利或习俗，在会议一般性理事会，尤其是法国的国家，以及那些schismatics和其他谁是未来的说拉特兰安理会通过共同或特殊的权利，考虑到申报或使徒信中我们的前辈或使徒见（当然，除非这些规定禁止），以及人员和同夥的人谁来，无论地位，职级，条件或贵族，他们可能会，教会或世俗的，为自己和所有财物，一个自由，保障和全面的safeconduct ，为今后的陆地或海上的国家，地区和地方，均受说，罗马教会，本拉特兰理事会在罗马，以及居住在城市和自由交换意见，并离开它往往是他们愿意，完全，不受限制和总的安全和真正的和不容挑战教皇的保证，尽管有任何强加的教会或世俗的谴责和惩罚，可能已经颁布了对他们的一般，不论原因，根据法律规定或上述看到，在任何形式的文字或条款，他们可能在一般性支出。

If an election is to be determined by a majority of a single vote, and that can be procured by a party through artifice or corruption, the Government may be the choice of a party for its own ends, not of the nation for the national good. If that solitary suffrage can be obtained by foreign nations by flattery or menaces, by fraud or violence, by terror, intrigue, or venality, the Government may not be the choice of the American people, but of foreign nations.

如果选举需由一人一票的多数票来决定胜负，而一个政党可以通过欺骗和腐蚀来达到目的，那么这个政府就有可能是政党为自身目的而做出的选择，而不是国家为全国利益而做出的选择；如果其他国家有可能通过奉承或胁迫，欺诈或暴力，通过恐怖、阴谋或收买等伎俩控制了这次选举，那么这个政府就可能不是美国人民做出的选择，而是其他国家做出的选择。

Not by rules and observances, not even by much learning, nor by gain of absorption, nor by a life of seclusion, nor by thinking,"I enjoy the bliss of renunciation, which is not experienced by the worldling" should you, O monks, rest content, until the utter destruction of cankers is reached.

比丘不应只是因为有戒行、或多闻、或有禅定、或独处、或自知"我得享凡夫享受不到的出离乐"而感到满足，而不灭尽烦恼。

The call developmental process of Psittacula agapornis consists of five instinct phases according to its call character. In the first phase （congenital cal1 phase, CCP, 0~12 day of age）, the juveniles only produce single syllable with short syllable length. The second phase（blank-model-forming phase,BMFP, 13~30 day of age） is important for formation of vocal learning pathways. During this phase, the calls characterized by more and longer syllables. In the third phase （call-model-forming phase,CMFP, 31~45 day of age）, the call development is guided by a model system for vocal learning, vocal learning is engendered so that some calls with multi-syllables with UP1 are produced, principal frequency has increased about by 920 cent, the number of note is double that of second phase. The short-term memories are transformed into long-term memories in the forth phase （call-feedback-learning phase,CFLP, 46~90 day of age）. The call of juveniles is a close match to tutor call which accompanied by double various sounds with BS and UP1, and more resonance components were formed. Second principal frequency has increased about by 970 cent, the number of note increases by 2.6~3.0 times. During the fifth phase （excellent call phase,ECP, after 91 d）, vocal motor pathway is developed and the calls are accompanied with most stable, harmonious vari-tone and complex repertoire.

牡丹鹦鹉的鸣声发育分为5个时期。0~12 d为先天性鸣声期，鸣声特征为以基本音为主音的单音节单音调声，声长短。13~30 d为空白模板形成期，鸣声特征为以基本音为主音的多音节单音调声，声长和音量显著性增加，表明发声学习通路开始形成。31~45 d为鸣声模板形成期，鸣声特征为以第1陪音（UP1）为主音的多音节单变调声，声长和音量显著性增加，主音频提升约920音分，涵盖的律音数增加1倍，表明发声学习模板逐渐形成，发声学习开始。46~90 d为鸣声反馈学习期，鸣声特征为以BS和UP1为主音的双变调声，声长和音量显著性增加，主音频涵盖的律音数增加2.6~3.0倍，第2主音频提升约970音分，表明稳定模板逐渐形成，短期记忆逐渐向长期记忆转化。91 d后为完美鸣声期，鸣声特征为以UP1为主音的复合变音调声，声长、音量、主音频的提升和涵盖的律音数都趋于平稳，鸣声稳定而和谐，表明发声运动通路基本形成。

"That the doctrine of the Reformed churches concerning predestination, and the points annexed to it, by its own genius and necessary tendency, leads off the minds of men from all piety and religion; that it is an opiate administered by the flesh and by the devil, and the stronghold of Satan, where he lies in wait for all; and from which he wounds multitudes, and mortally strikes through many with the darts both of despair and security; that it makes God the author of sin, unjust, tyrannical, hypocritical; that it is nothing more than interpolated Stoicism, Manicheism, Libertinism, Turcism; that it renders men carnally secure, since they are persuaded by it that nothing can hinder the salvation of the elect, let them live as they please; and therefore, that they may safely perpetrate every species of the most atrocious crimes; and that, if the reprobate should even perform truly all the works of the saints, their obedience would not in the least contribute to their salvation; that the same doctrine teaches, that God, by a mere arbitrary act of his will, without the least respect or view to sin, has predestinated the greatest part of the world to eternal damnation; and, has created them for this very purpose; that in the same manner in which the election is the fountain and cause of faith and good works, reprobation is the cause of unbelief and impiety; that many children of the faithful are torn, guiltless, from their mothers' breasts, and tyrannically plunged into hell; so that, neither baptism, nor the prayers of the Church at their baptism, can at all profit by them;" and many other things of the same kind, which the Reformed Churches not only do not acknowledge, but even detest with their whole soul.

&该学说的革新教会联谊会关于宿命，并分附在它，由它自己的天才和必要的倾向，导致起飞的头脑男子从所有虔诚与宗教；这是一个鸦片类由血肉所魔鬼和据点撒旦，在那里他是在等待所有；从他的伤口众多，而致命打击，通过许多与飞镖都绝望与安全；因为它使上帝的作者单，不公正，专横，虚伪的，即它只不过是多插生活俭朴，摩尼教，放纵， turcism ；这使得男性的肉体关系稳固，因为它们是说服它说，没有任何东西可以阻碍救赎的选举，让他们住，因为他们请；因此，他们可以安全地胡作非为，每一个物种的最残暴的罪行，并认为，如果reprobate甚至应该真正履行全部工程的圣人，他们的服从，将不会在至少贡献出他们的救恩，即同一教义教导说，上帝，由一个单纯的任意行为，他的意志，如果没有起码的尊重，或以单，有predestinated最大的是世界的一部分，以永恒damnation ，并且创造了，他们为了这个目的，即在同一何种方式选举是喷泉和事业的信念和良好的工程， reprobation ，是导致不信者和不虔诚；说，许多儿童的教友们被拆除，无罪，从他们的母亲的乳房，并tyrannically陷入地狱，因此，既不洗礼，也没有祷告的教会，在他们的洗礼，能在所有利润由他们来主宰；&和许多其他东西的同类，而改革教会不仅不承认，但即使是讨厌自己的整个灵魂。

The growth inhibiting rate of T24 cell lines were detected by MTT methods, apoptosis of cells were detected by flow cytometry, the mechanism of apoptosis was analyzed by detecting the protein expression of Bcl-2, Bax, Caspase-9, Caspase-3 and cytoplastic protein Cytochrome C. 4 We injected live T24 cells into the subcutaneous space of nude mice and successfully built up the animal model of bladder carcinoma. The effect of CS-PAA-EPI polymer magnetic microspheres targeting chemotherapy was investigated by HE staining, TUNEL ,tumor weight and volume inhibition rate. Results: 1 TEM revealed that the CS-PAA polymer magnetic microspheres were regular spherical shape,the average diameter was 80nm in dry condition. By controlling the pH value of the medium,polymers had positive or negative zeta potential. VSM showed the CS-PAA polymer magnetic microspheres had superparamagnetic. The diameter of CS-PAA-EPI polymer magnetic microspheres were 200nm in solution by DLS examining,the embedding ratio was 20%,the EPI loading rate was 15%, which was higer than reported in other articles. 2 Raw eye observation found that the rat"s bladder of treatment group was brown color,which meaned the aggregation of iron particles, compared with the control group, iron stain found iron particles were assembled in rat"s bladder of the treatment group, the amount of iron particles in liver and spleen were less obviously.

研究结果：1合成的CS-PAA磁性聚合物微球呈球形，大小均一，TEM测定其干态下粒径为80nm左右，磁化曲线证实具有超顺磁性，具有一定的PH敏感性，固载表柔比星后，水溶液性状稳定，无沉淀物，DLS测定直径约200nm左右，测定载药率为15％，较文献报道高，包封率为20％。2肉眼观察试验组大鼠膀胱表面呈褐色，可见大量的Fe粒子聚集，普鲁士兰染色法显示，试验组大鼠膀胱壁内有大量的Fe粒子，分布至膀胱壁全层，与对照组大鼠相比，试验组大鼠的肝、脾内的Fe粒子聚集量明显降低；HPLC测定结果与Fe染色相同；高剂量磁性CS-PAA-EPI生理盐水组及单纯EPI生理盐水组均在给药后14天出现血肌酐和尿素氮的升高，其他组大鼠血生化指标没有明显变化。3MTT法发现，高、中、低剂量磁性CS-PAA-EPI生理盐水组在外加磁场的协同作用下杀伤T24细胞效应明显高于单纯的EPI生理盐水组，FCM发现试验药物组可引起明显的肿瘤细胞凋亡，试验药物治疗组细胞胞浆内出现了由线粒体释放出的细胞色素C，试验组细胞Bcl-2蛋白减少，Bax蛋白变化不明显，Caspase-3、Caspase-9蛋白受到了激活活化。4高、中、低剂量磁性CS-PAA-EPI生理盐水组的瘤重抑制率和瘤体积抑制率均明显高于单纯的EPI生理盐水组(P＜0.01)，其中高剂量组的抑制率最高。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。