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METHODS AK was purified by DEAE ion-exchange cellulose(DEAE-52) and sepharose Cl-4B .The component of the saccharide was determined by GC and TLC.Its MW was measure d by gel filtration.The form of glucosidic bond was detrermined by IR.The conten t of the saccharide was measured by colorimetry.

采用DEAE纤维素(DEAE-52)柱层析和凝胶柱层析分离纯化草乌多糖，气相色谱法和薄层层析法测定其多糖组成，凝胶过滤法测定其相对分子质量，红外光谱测定其苷键类型，比色法测定其糖含量。

In this study, different nano-hydroxyapatite particles,HAP_1(25-60nm), HAP_4(the additives is heparin, 15-50nm), HAP_5(the additives is bovine serum albumin BSA, 20-80nm) were prepared by homogeneous precipitation and used heavy-gauge hydroxyapatite as comparison,we determined amount of heparin, sialic acid ,BSA adsorbed on HAP_1,HAP_2,HAP_3 by Crystal Violet assay, Bialsche method,Bradford method respectively and analyzed the binding mechanism by infrared spectrum;After taking HAP_1,HAP_2,HAP_4,HAP_5 and RBC to co-culture in vitro,we studied RBC hemolysis test and detected RBC hematolysis rate by erythrocyte osmotic fragility test;observing the changes of morphous and locomotion of cell after coacting HAP_1,HAP_2,HAP_4,HAP_5 and RBC by light microscope and inverted phase contrast microscope;observing HAP_1,HAP_2,HAP_4,HAP_5 effecting on Ultrastructure of RBC.

本文用均匀沉淀法制备了HAP_1(25-60nm)，HAP_4(添加剂肝素，15-50nm)，HAP_5(添加剂牛血清白蛋白BSA，20-80nm)等纳米粒子，并用大尺寸的羟基磷灰石HAP_2(470-520nm)，HAP_3(1906nm)作对照，分别利用结晶紫法、Bialsche法、Bradford法研究了肝素、唾液酸、血清白蛋白在HAP_1，HAP_2，HAP_3上的吸附量，用红外光谱分析其中的结合机理；在体外将HAP_1，HAP_2，HAP4_，HAP_5与红细胞共培养，进行了红细胞溶血试验的研究，并借助红细胞渗透脆性试验检测红细胞溶血率；运用普通光学显微镜和倒置相差显微镜观察了HAP_1，HAP_2，HAP_4，HAP_5与红细胞作用后细胞形态及运动的变化：透射电镜观察了HAP_1，HAP_2，HAP_4，HAP_5对红细胞超微结构的影响。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Soil Ca2+ content increased by 0.749mmol/kg, Mg2+ content increased by 0.169mmol/kg, the Na++ K+ content dropped by 9.52mmol?kg, SO42- increased by 0.091mmol?kg, Cl- reduced by 1.727mmol?kg, the HCO3- content in the soil assumed the obvious dropping tendency, it might be explained that the advancement of HCO3- has transformed into CO32- in the oasis, and has formed precipitation with two price calcium magnesium heteropolar ,then fix in the soil layer.

4土壤Ca2+含量增加了0.749mmol/kg，Mg2+含量增加了0.169mmol/kg ，Na++K+含量下降了9.52 mmol∕kg，SO42-增加了0.091 mmol∕kg， Cl-降低了1.727 mmol∕kg，土壤中HCO3-的含量呈明显下降趋势，可解释为在绿洲化进程中HCO3-转化为CO32-，并与二价钙镁离子结合形成沉淀固定于土层中。

According to the variation in the components of intermediateacid igneous rocks coming from the lower crust, it is possible to restrain the depth of the source area and the minimum thickness of the crust, and thus to provide important information for the study of the deep process of the continental intraplate mineralizationGlobally, many world level porphyry copper deposits and metallogenic systems formed by hypabyssal hot liquid have their close relationship with the synchronous adakite in spacetime and genesis; domestically, the adakitelike rocks have been identified to be related to the metallogenesis in the main metallogenic areas in ChinaThe recognition of adakitelike rocks having no relationship with the process of subduction makes it possible to construct a metallogenic model of continental intraplate porphyry metal deposits by combining other geological evidences, and this model is totally different from the metallogenic model of porphyry copper deposits with Bsubduction setting constructed by Sillitoe (1972)The existence of adakitelike rocks may be the necessary condition but not the sufficient one for forming the largescale porphyry deposits and the hypabyssal hotliquid deposits, whose metallogenic elements mainly came from the mantleThe metallogenic potential of adakitelike rocks is achieved by the entering of the mantle material, and the metallogenic specialization of adakitelike rocks is decided by the distribution characteristics of the metallogenic elements in the upper mantleAn important reason for the adakitelike magma related to subduction being advantageous to mineralization is that there were abundant high pressure and high temperature liquid coming from the subducted platepieces and the magma of high fO2 coming from the melting of subducted platepiecesHowever, for those adakitelike rocks, whose occurrence has continental plate background but does not relate to the subduction, their metallogenic mechanism is not clear yetBased on the concept of modern mineral exploration and combined with the analysis of integrated geological information, we may better realize the practical prospecting significance of the adakitelike rocks

根据起源于下地壳的中酸性岩浆岩的成分变化，可以约束其源区深度以及地壳最小厚度，为大陆板内成矿作用的深部过程研究提供重要信息。全球范围内，许多世界级斑岩铜矿和浅成热液矿化系统与同期的埃达克质岩存在密切的时空与成因联系，在国内主要成矿区带也识别出与金属成矿作用有关的埃达克岩。与俯冲过程无关的埃达克质岩的识别，使我们有可能结合其他地质证据构建完全不同于Sillitoe(1972)B型俯冲环境的斑岩铜矿成矿模式的大陆板内斑岩型金属矿床成矿模式。对于规模巨大、矿质主要源自地幔的热液矿床的形成，埃达克岩可能是必要条件，但不是充分条件。埃达克岩的成矿潜力通过地幔物质加入而获取，埃达克岩的成矿专属性由上地幔成矿元素分布特征决定。与俯冲有关的埃达克质岩浆之所以有利于成矿，重要的原因是存在大量来自俯冲板片的高压、高温流体以及俯冲板片熔融形成高氧逸度(fO2)的熔体，但产出在大陆板内背景、与俯冲无关的埃达克岩的成矿机制还不清楚。根据现代资源勘查理念，结合综合地质信息分析，埃达克质岩具有实际找矿意义。

Methods: Animals were randomly classified into 5 groups:gaster-poured by physiological solution; gaster-poured by ethanol; gaster-poured by ethanol after hypophrenic vagotomy; gaster-poured by ethanol after big-small splanchnicotomy; gaster-poured by ethanol after hypophrenic vagotomy and big-small splanchni-cotomy.

动物被随机分为5组，每组10只。A组：单纯生理盐水灌胃组；B组：单纯乙醇灌胃组；C组：膈下迷走神经切断乙醇灌胃组；D组：内脏大、小神经切断乙醇灌胃组；E组：膈下迷走神经和内脏大、小神经均切断乙醇灌胃组。

In order to reasonably review the status and progress of ESCR in China, we introduce some methods measuring human rights, for example, HDI developed by UNDP(2000) based on progressive realization, a violations approach by Chapman (1996),a subsistence rights approach by Apodaca（2005）, a three-dimension approach by Landman(2004), and so on, and measure the right to education, health, and labor by right indicators.

为了准确评价中国的经济、社会和文化权利的现状和变化，我们还引入了Chapman (1996)、UNDP(2000)、 Landman(2004)和Apodaca（2005）等发展的人权度量理论和方法，试图从教育权、健康权和劳动权三种权利的视角建立各自的权利指数，通过指数来度量这些权利的总体变动情况。

The effect of F 1C on joint swelling and the immune respones of AA rats were evaluated by lymphocytotic reaction, flow cytometry, and ELISA. Results F 1C by ig administration to AA rats decreased the primary joint swelling of AA rats, stronger than TW and weaker than CsA, promoted the decreased splenocyte proliferation induced by Con A, and inhibited the augmented thymocyte proliferation induced by Con A in AA rats. The evaluation in vitro showed the F 1C inhibited the augmented thymocyte proliferation from AA rats and promoted the deficient IgG produced by splenocyte from AA rats.

结果 ig给予F1C(30mg／kg)对AA大鼠原发性足肿胀具有明显的治疗作用，该作用较TW强，较CsA弱；F-1C对AA大鼠低下的脾细胞增殖反应具有改善作用；对亢进的胸腺细胞增殖反应具有抑制作用。F-1C体外对AA大鼠低下的脾细胞产生IgG水平具有明显的促进作用，并能抑制亢进的胸腺细胞增殖反应。

Hence too both Tragedy and Comedy are claimed by the Dorians as their discoveries; Comedy by the Megarians--by those in Greece as having arisen when Megara became a democracy, and by the Sicilian Megarians on the ground that the poet Epicharmus was of their country, and a good deal earlier than Chionides and Magnes; even Tragedy also is claimed by certain of the Peloponnesian Dorians.

因此多利安人宣称悲剧和喜剧都是他们的发明；喜剧由西西里的麦加拉人发明，理由是诗人厄皮卡玛斯是他们国家的人，这时希腊已经兴起，麦加拉建立了民主制度，这比奇奥里德斯和麦克赖斯要早很多；甚至一些伯罗奔里萨的多利安人声称悲剧也是他们的发明。

Method］from january 2003 to may 2006,32 patients were corrected with qin si-he's orthotics devices on the ilizarov principle of tension-stress,which involved 15 males and 17 females,the age ranged from 10 to 25 years.among these patients,2 were caused by peroneal nerve injury,l by tumor in the vertebral canal,5 by meningocele,11 were caused by poliomyelitis,13 by congenital talipes equino-varus.in accordance with deformities,external fixator and limitied operative methods were dertermined.the limited release of soft tissue were performed in 7 patients,limited osteotomy in 25 patients.the dynamic muscle balance operation were performed in 9 patients with imbalance of muscle strength.according to the ilizarov technique,the fixative rods were installed.the telescopic rods on the apparatus were rotated one week after the operation,the divices had corrective function in three-dimensional directions.the deformity of talipes equinovarus,internal rotation and drooping of the forefoot were gradually corrected,and the patients could bear weight and walked on the deformed foot.the mean duration of traction were 42 days,then removed the external fixator maintained with plaster for a site time.

方法］2003年1月～2006年5月，根据ilizarov张力应力法则，应用秦泗河改良的外固定矫形器，遵循ilizarov穿针固定的基本原则，共手术治疗马蹄内翻足32例，男15例，女17例；年龄10～25岁，平均17岁。病因：腓总神经损伤2例，腰椎管内肿瘤1例，硬脊膜膨出5例，小儿麻痹后遗症11例，先天性马蹄内翻足13例。术前用足掌的前外缘负重行走者11例，用足的外缘或足背外侧负重者21例。根据马蹄内翻足畸形程度、性质和患者年龄，确定实施有限矫形手术的方法和外固定矫形器治疗。本组7例同期实施有限的软组织松解术，25例同期实施了有限的截骨术和跗骨间关节融合术，9例合并踝关节内外翻肌力明显失衡者，同期行足部肌腱转移的肌力平衡术。然后安装外固定矫形器。术后按作者制定的管理程序，7 d开始旋转相应的螺纹牵拉杆，对器械进行三维空间的缓慢调整，先矫正前足内收和后足内翻，后矫正足下垂畸形，直至达到矫形要求的标准。在矫形的过程中定期进行x线检测，以防止发生踝关节前后移位，治疗期间允许患足负重行走。术后平均牵伸42 d，拆外固定器后患足再上石膏固定适当时间。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷