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The principal purpose of this study is to set up the isolation methods for diphtheria toxin expressed by genes, to purify the toxin with complete biological activities, and to make researches on its toxicities. In the experiments, the target gene was inserted into the cloning vectors--pET22b, then the vectors were introduced into host cells -BL21(DE3). The expression of the toxin was induced by addition of IPTG. The expression products were purified by His-6 label affinity chromatography. Then the diphtheria toxin expressed was transferred to PVC membrane using semidry blotter to determine the amino acid sequence of N-terminal. The "nicked" diphtheria toxin linked by disulfide bond was gained by protease hydrolysis.

为建立重组基因表达白喉毒素的分离纯化方法，制备出具有生物活性的蛋白质毒素，构建了白喉毒素表达载体pET22b-DT，并转入大肠杆菌BL21(DE3)中诱导表达，以融合表达的His-6作为标签通过亲和层析纯化；表达毒素经蛋白酶酶切后，形成了以二硫键连接的与天然结构相同的带"缺口"的白喉毒素，并测定白喉毒素对豚鼠的急性毒性和细胞毒性。

The seeds of discontent were planted by Adolph Berle and Gardiner Means (1932) in relation to the separation of ownership from control, by Ronald Coase (1937) with reference to the puzzle of vertical integration and the theory of the firm, by Chester Barnard (1938), who featured adaptation of a cooperative kind accomplished through hierarchy (as opposed to adaptation through the market in response to changes in relative prices), by the 'marginalist controversy' of the 1940s, and by Armen Alchian's (1950) appeal to evolutionary arguments to explain observed economic regularities.

不满的种子由此而种下，例如， Adolph Berle 和 Gardiner Means （1932）有关所有权和控制权分离的研究， Ronald Coase （1937）对纵向一体化难题和企业理论的关注， Chester Barnard （1938）对一种通过层级实现的合作式调节方式（相对于通过市场响应相关价格改变而实现的调节方式）的强调，20世纪40年代的"边际主义论战"，以及 Armen Alchian （1950）对用演进观点来解释观察到的经济规律的呼吁。

The main representations for agriculture industrialization to improve our agriculture economy are: first, to solve the contradiction between the small production of our agriculture and big international market; second, to heighten the comparative efficiency of agriculture by specialization of production; third, to promote the process of urbanization by widening the traditional industrial chain. Fourth, to solve the contradiction between specializationproduction and social serves by making foreign trade systems of agriculture goods sound. fifth, to raise diathesis of farmers by spreading agriculture techniques; sixth, to promote standardization and internalization of agriculture by making the standardization system.

农业产业化经营促进我国农业经济发展的主要表现是：第一，解决了我国农业小生产和国际大市场之间的矛盾；第二，实现了专业化生产，提高了农业的比较效益；第三，拓展了传统的产业链，促进了农村城镇化进程；第四，健全了农产品外贸流通体制，解决了专业化生产和社会化服务之间的矛盾；第五，促进了农业科技的推广，提高了农民的素质；第六，健全了农业标准体系的建设，促使农业走向规范化、标准化、国际化。

The stable and reliable operation voltage of the probe can be given by the following methods:(1) 20% light coupling efficiency is obtained by direct coupling of LD-optical fiber.(2) The light power is transmitted by multi-model optical fiber.(3) The largest photo-electric conversion efficiency is achieved by selecting the transformer regulation of step-up transformer.(4) The stable light intensity is achieved by using LD light feedback of driving circuit and refrigeration.

通过下述方法可提供稳定、可靠的探头工作电压：LD—光纤的直接耦合方式，可实现20％左右的光耦合效率；采用多模光纤传输功率；选择升压变压器最佳变比提高光电转换效率；采取LD驱动电路光反馈以及致冷措施使其发光幅值稳定。

The enterprise stock rights owned by asset management companies include: stock rights obtained by asset management companies through debt-to-equity swap, stock rights possessed by asset management companies after the debt enterprise being restructured, and stock rights possessed by asset management companies by other means

资产管理公司拥有的企业股权，包括：资产管理公司对企业实施债转股后取得的股权，资产管理公司对欠债企业进行重组后拥有的股权，资产管理公司以其他方式拥有的股权

The prizes for physics and chemistry shall be awarded by the Swedish Academy of Sciences; that for physiological or medical works by the Caroline Institute in Stockholm; that for literature by the Academy in Stockholm; and that for champions of peace by a committee of five persons to be elected by the Norwegian Storting.

物理学奖和化学奖将有瑞典科学院颁发；生理学和医学奖将由斯德哥尔摩的凯若琳学院颁发；文学奖将由斯德哥尔摩的瑞典文学院颁发；和平奖的患上主将由挪威国会推选出的五人委员会决定。

The prizes for physics and chemistry shall be awarded by the Swedish Academy of Sciences; that for physiology or medical works by the Karolinska Institute in Stockholm; that for literature by the Academy in Stockholm, and that for champions of peace by a committee of five persons to be elected by the Norwegian Storting.

物理学及化学奖将由瑞典科学院授予；生理学或医学奖将由斯德哥尔摩的卡罗琳斯卡医学院授予；文学奖将由斯德哥尔摩的学院授予；而和平战士（注：champions of peace，诺贝尔的原文）奖由挪威国会选出的5 人委员会授予。

The prizes for physics and chemistry shall be awarded by the Swedish Academy of Sciences; that for physiology or medical works by the Karolinska Institute in Stockholm; that for literature by the Academy in Stockholm; and that for champions of peace by a committee of five persons to be elected by the Norwegian Storting.

物理奖和化学奖由斯德哥尔摩瑞典科学院颁发；医学和生理学奖由斯德哥尔摩卡罗琳医学院颁发；文学奖由斯德哥尔摩文学院颁发；和平奖由挪威议会选举产生的5人委员会颁发。

Renewing the decision taken by our predecessor of happy memory, Innocent, we decree that the ordinances made by the heresiarchs Octavian {2 } and Guido {3 }, and also by John of Struma {4 } who followed them, and by those ordained by them, are void; and furthermore that if any have received ecclesiastical dignities or benefices through the foresaid schismatics, they are to be deprived of them.

续约的决定所采取的前身快乐记忆，无辜的，我们的法令条例，由heresiarchs octavian （ 2 ）和 Guido ，同时由约翰的甲状腺肿（ 4 ）谁也随之，由这些受祝圣他们的，均属无效；此外，如果任何已收到教会尊严或benefices通过foresaid schismatics ，他们被剥夺了他们。

In section 1.3, taking the advantage of the classification by the index arrays ,pointed out the space spanned by class A,B,C respect!vivety are subalgebras of NQ. We also show that the subspace spanned by the elements of length 2 in C is an abelian subalgebras of NQ,denoted by H.we find H is contained in the center of subalgebra P of the subspace spanned by the elements in class C.

在1.3节中，应用指标数组我们讨论了各类元素的性质，指出其第A，B，C类元各自张成的子空间均是N_Q的子代数，特别是C类中所有长度为2的元素张成了N_Q的Abel子代数H，且含于C生成的子代数P的中心Z中。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。