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West Virginia is bordered by Pennsylvania to the north; by Ohio to the north and west; by Kentucky to the west; by Maryland to the north and east; and by Virginia to the east and south.

西弗吉尼亚州接壤的东北部宾夕法尼亚州；由美国俄亥俄州的北部和西部；由肯塔基州以西的马里兰州的北部和东部地区；和弗吉尼亚州东部和南部。

In another word, the status does not exist that a painter should learn from nature "purely" without any "prejudice" and paint realistically by facing an object to paint out what he or she has perceived. To obtain such capability, painting learners or students shall use their eyes (teachers ask students in the painting class "where are your eyes and your perceptions") to observe objects and things at the beginning of study and then paint the models faithfully by means of "facade-subdivision"(or "preferring to square not round" as described by Xu Beihong, or decomposing the object into many petty facades as described in the line drawing textbook by P.P. Chistjakov so as to turn around the object bit by bit).

也就是说，不存在这样一种状态，画家为了画出自己的感觉，他们应该"纯洁"地不带"偏见"地去向自然学习，去面对物体直截了当地写生，而为了获得这种能力，在学习绘画的初始阶段，就必须训练学生们用自己的眼睛(在绘画课室里，老师总是质问学生，你的眼睛在哪里，你的感觉在哪里)去观察对象，然后用一种"分面"的方法(也就是徐悲鸿所说的"宁方勿圆"，或者按照据说是契斯恰科夫的素描教程的要求把对象分解成许多小面，以便使物体能够一点一点地转过去的方法)把眼前的模特如实地画下来。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

The Shah Dynasty found then lasted until now. Nepal was invaded by Britain in 1814 and forced to cede a vast expanse of territory to India which was controled by Britain at that time. Its diplomacy was supervised by Britain as well. During the period from 1846 to 1950 Nepal was controled by Rana Family . The fight aganist it intrigued by Nepali Congress brought the dormination of Rana Family to an end, and constitutional monarchy was put into practice. In February of 1959 ,Mahemdra Bir Bikram Shah Dev issued the first constitution of Nepal, the New Constitution was issued in 1962. Birendra , Bir Bikram Shah Dev ascended throne in1972, he dismissed the National Panchayat in the April 16, 1990,and he also issued the third constitution in Nov ember of the same year. Then the multi-party of constitutional monarchy was set up.

建立了沙阿王朝，延续至今。1814年英国入侵，尼泊尔被迫割让大片领土给英属印度，外交受英监督。1846年―1950年为拉纳家族统治时期。1950年11月，尼泊尔大会党等发起反拉纳的斗争，结束了拉纳的统治，并实行君主立宪制。1959年2月马亨德拉颁布尼泊尔第一部宪法。1962年颁布新宪法。1972年比兰德拉国王即位。1990年4月16日，比兰德拉国王解散全国评议会，同年11月颁布第三部宪法，实行君主立宪的多党制。

The outcome measures included muscle spasticity of paretic upper limb by Modified Ashworth Scale, grasp strength of both hands by JAMAR dynamometer, functional performance of paretic upper limb by Action Research Arm Test, daily use and movement quality of paretic upper limb by Motor Activity Log-28, and motor function by Brunnstrom upper limb recovery stage.

疗效评估包含—使用修正式艾许瓦氏等级量表评估患侧上肢肌痉挛程度、使用JAMAR握力计评估双手握力、使用功能研究手臂测试评估患侧上肢功能性表现、使用动作活动日志（Motor Activity Log-28）评估受试者患侧上肢的日常使用量及动作品质、使用布郎斯壮上肢恢复分期（Brunnstrom Upper Limb Recovery Stage）评估患侧上肢的动作功能。

The five paintings include Le Pigeon aux Petits Pois by Picasso, Pastorale by Matisse, The Olive Tree near Estaque by Georges Braque, Woman with a Fan by Amadeo Modligiani, and Still Life with Chandeliers by Fernand Léger.

这五幅画作包括毕加索的《鸽子与豌豆》，马蒂斯的《田园曲》、乔治·布拉克的《埃斯塔克附近的橄榄树》、莫迪格里阿尼《持扇的女人》和莱格尔的《静物·吊灯》。

The evolutionary relationships were investgated by comparing the results from molecular data alone or molecular data combined morphological characters using different analysis methods: maximum parsimony by PAUP(superscript *) 4.0 and Bayesian inference by MrBayes 3.0B4. Base composition and ti/tv of 28S rDNA D2 regions were analyzed by PAUP(superscript *) 4.0. The results showed that GC content of the 28S rDNA D2 gene sequences of subfamily Euphorinae ranges from 40.00% to 49.25%. Transversion frequency of sequence variable sites among species of Euphorinae was higher than that of transition. The subfamily Euphorinae was proved to be a paraphyletic group with close relationship to subfamilies Neoneurinae and Cenocoelinae; only two tribes, Meterorini and Microctonini, were proved to be monophyletic groups; Centistini, Cosmophorini, Euphorini and Dinocampini were paraphyletic groups; the basal position of tribe Meterorini within Euphorinae was partially supported by our phylogenetic trees, and tribe Microctonini was proved to be a group at the top of the trees.

结果表明：优茧蜂亚科的28S rDNA D2基因序列片段的GC%含量在40.00%~49.25%之间变动，而对于碱基替代情况来讲，优茧蜂亚科各个成员间序列变异位点上颠换大于转换；不同的分析和算法所产生的系统发育树都表明目前根据形态定义出的优茧蜂亚科Euphorinae不是一个单系群，而是一个与蚁茧蜂亚科Neoneurinae和高腹茧蜂亚科Cenocoelinae混杂在一起的并系群；在优茧蜂亚科内部，悬茧蜂族Meterorini和食甲茧蜂族Microctonini（排除猎户茧蜂属Orionis）为单系群，而宽鞘茧蜂族Centistini、大颚茧蜂族Cosmophorini、优茧蜂族Euphorini、瓢虫茧蜂族Dinocampini为并系群；悬茧蜂族Meterorini在优茧蜂亚科Euphorinae内位于基部位置的观点得到部分的支持，同时食甲茧蜂族Microctonini被判定为相对进化的类群。

Methods The rabbit model with hyperhomocysteinemia was established by L-methionine subcutaneouse injection. The changes of white microthrombus and microvascular diameter in pia mater microvessel were observed by using cranial window method and closed microcirculation detection system, The concentration of Hcy was detected by high performance liquid, The level of von Willebrand factor, endothelin-1 (ET-1) were assayed by ELISA. The content of NO was measured by nitrate reduction method. Meanwhile, the relationship between the changes of microvascular diameter and blood concentration ET-1, NO was analyzed.

采用L-蛋氨酸皮下注射法建立HHcy家兔模型，应用"颅骨窗口法"和微循环闭路检测系统观察其软脑膜微血管中白微栓数量及微血管口径变化；采用柱前衍生反相高效液相色谱－荧光法检测同型半胱氨酸浓度；平行观测血管内皮功能指标血管性血友病因子抗原（vWF，用酶联免疫吸附法）、血浆内皮素－1ET-1，用ELISA）、血清一氧化氮水平；并对HHcy所致ET-1、NO血液浓度变化和微血管口径变化进行相关分析。

The results show that ponceau 4R excited by light at the wavelength of 330-430 nm can generate a strong fluorescence at the 621 nm peak wavelength with its best excitation wavelength being 376 nm, amaranth excited by light at the wavelength of 300-440 nm can generate a strong fluorescence at the 643 nm peak wavelength with its best excitation wavelength being 370 nm, tartrazine excited by light at the wavelength of 280-380 nm can generate a strong fluorescence at the 565 nm peak wavelength with its best excitation wavelength being 315 nm, sunset yellow excited by light with wavelength of 310-410 nm can generate a strong fluorescence at the 592 nm peak wavelength with its best excitation wavelength being 348 nm, and brilliant blue excited by light at the wavelength of 320-390 nm can generate a strong fluorescence at the 456 nm peak wavelength with its best excitation wavelength being 350 nm.

结果表明，胭脂红在波长330～430 nm的光激发下，产生较强荧光，荧光峰值波长为621 nm，最佳激发波长为376 nm；苋菜红在波长300～440 nm的光激发下，产生较强荧光，荧光峰值波长为643 nm，最佳激发波长为370 nm；柠檬黄在波长280～380 nm的光激发下，产生很强荧光，荧光峰值波长为565 nm，最佳激发波长为315 nm；日落黄在波长310～410 nm的光激发下，产生较强荧光，荧光峰值波长为592 nm，最佳激发波长为348 nm；亮蓝在波长320～390 nm的光激发下，产生较强荧光，荧光峰值波长为456 nm，最佳激发波长为350 nm。

I do not know how the operation and nature of the ancient Patria Potestas can be brought so vividly before the mind as by reflecting on the prerogatives attached to the husband by the pure English Common Law, and by recalling the rigorous consistency with which the view of a complete legal subjection on the part of the wife is carried by it, where it is untouched by equity or statutes, through every department of rights, duties, and remedies.

我们只要回想一下纯粹英国普通法所赋与丈夫的各种特权，并回想一下，在普通法还没有经衡平法或制定法修正的部分中，在权利、义务和救济等各方面严格地坚持妻在法律上必须完全从属的见解，我们将对古代"家父权"的运用和性质，获得一个鲜明的印象。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了